• 한국응용곤충학회지
• /
• 제48권1호
• /
• pp.87-94
• /
• 2009

멀구슬나무와 고삼 식물추출물을 활용한 KNI3126의 주요 해충 6종에 대한 살충효과와 천적류에 대한 영향평가를 하였다. 벼멸구에 대한 KNI3126의 살충효과는 약제처리 후 5일째에 95% 이상이었으며, 목화진딧물은 처리 후 3일째에 95% 이상을 나타냈지만, 5일째에는 다소 살충효과가 떨어졌다. 오이총채벌레에서는 68.1%로 일반 살충제처럼 높은 살충효과를 나타내지는 않았다. 배추좀나방의 경우는 95% 이상의 높은 살충율을 나타내어 친환경자재로서 난방제 해충의 밀도 억제가 가능할 것으로 판단되었다. 점박이응애에서는 살포 1일째부터 80% 정도의 살비율을 보여 5일째에는 90% 이상의 높은 살비율을 나타냈다. 포식성 천적인 이리응애류에 대한 영향평가 결과 국제생물방제협회(IOBC)의 생존율 40%이하인 선택독성이 중간정도로 판정되었으며, 기생성 천적의 경우에는 비교적 안전한 것으로 평가되었다. 벼멸구에 대한 기피효과는 약한 것으로 판단되었지만, 점박이응애에 대해서는 높은 것으로 판명되었다. 또한, 안전성을 검토한 결과 급성경구, 급성경피, 어독성에서 저독성으로, 피부자극성과 안점막자극성시험에서는 자극이 없는 것으로 나타났다.

• 한국입자에어로졸학회지
• /
• 제18권2호
• /
• pp.37-50
• /
• 2022

Due to the syndrome coronavirus 2 (SARS-CoV-2) pandemic, the subway passenger cabin should be continuously sterilized. However, a disinfectant such as chlorine is toxic and can lead to different issues to human health. In this paper, we introduced a novel disinfectant based on natural product (Dendropanax morbifera extract). Via ultra-high performance liquid chromatography - mass spectrometer (UHPLC-MS), different compounds from Dendropanax morbifera extract showed antivirus potentials. Antimicrobial experiments confirmed that the air-disinfectant containing Dendropanax morbifera can eliminate harmful microorganisms including Gram (-), Gram (+), and yeast within 5 mins. The as-prepared air-disinfectant also showed high antivirus activity against H1N1, HRV, and EV71. Deodorization test also indicates that the as-prepared air-disinfectant can lower the harmful gas such as ammonia and trimethylamine in the atmosphere. To evaluate the potential of air-disinfectant containing Dendropanax morbifera in practical applications, different safety tests including acute oral toxicity, acute skin irritation, and eye irritation were conducted. Results showed that the as-prepared disinfectant did not negatively affect tested animals during these safety investigations.

• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 1997년도 춘계학술대회
• /
• pp.115-115
• /
• 1997

A new capsaicin analog modified with 4-hydroxyl and alkyl chain of capsaicin was a very potent antiinflammatory analgesic drug and may be clinically useful for those who have rheumatoid arthritis, diabetic neuropathy and cancer. The purpose of this study was to investigate histopathology after short and long term application of poloxamer-based gels, and percutaneous absorption of various topical formulations. Poloxamer-based gel was prepared by cold method using poloxamer 407. The poloxamer gels was applied to dorsal sites of hairless mouse skin during one week or one month for the evaluation of skin irritation. The applied site was then sectioned for histopathologic examination. The topical formulations were also prepared using CMC, HPMC, MC, carbopol and glycerylmono stearate. Skin variation of poloxamer gels was studied using excised hairless mouse, rat, hamster and human penis skin. Franz-type diffusion cells were used far skin penetration of drug against receptor phase filled with about 10$m\ell$ of 0.9% saline solution kept at 32$^{\circ}C$. The concentration of drug was determined by the reverse phased C18, Symmetry HPLC with fluorometeric detector. No skin erythema was observed after dorsal application of poloxamer-based gels for one week or one month. No histopathologic changes was also examined, suggesting no skin toxicity of poloxamer-based gels. The order of flux rate was HPMC > MC ( CMC > poloxamer >> glycerylmono stearate ( carbopol. There was a skin variation of poloxamer gels. The flux rate of poloxamer gels was highest in case of hairless mouse followed by rat, human and hamster skin. The Partial support-Ministry of Science and Engineering (HAN project).

• 한국응용과학기술학회지
• /
• 제34권1호
• /
• pp.41-49
• /
• 2017

본 연구는 우엉 뿌리 추출물의 항산화 활성 및 피부에 대한 안전성을 평가하기 위하여 총 폴리페놀 함량, 총 플라보노이드 함량, DPPH radical 소거 활성을 통하여 항산화 활성을 살펴보고, B16F10 melanoma 세포에 대한 세포 독성 및 자외선 A에 대한 피부 세포 보호 효과를 확인하였다. 또한 화장품 소재로서의 활용을 검증하기 위하여 1차 피부 첩포 테스트를 실시하였다. 본 실험 결과 우엉뿌리 추출물의 함량이 증가됨에 따라 높은 폴리페놀과 플라보노이드의 함량이 확인되었으며, DPPH radical 소거 활성을 확인하였다. B16F10세포에 대한 세포 독성을 확인한 결과 B16F10 melanoma 세포에 대해 독성이 낮고, 자외선 A에 대해 80% 이상의 세포 보호 효과를 확인하였다. 또한 1차 피부 첩포 테스트를 통해 우엉 뿌리 추출물이 피부에 자극이 거의 없음을 확인하였다. 이러한 결과를 통하여 우엉뿌리 추출물이 항산화 활성과 자외선에 대한 피부 보호 효과가 뛰어나고 피부 세포에 대한 독성이 낮으며, 피부에 대한 안전성이 확인됨에 따라 화장품 소재로서의 가능성을 확인하였다.

• 한국미생물·생명공학회지
• /
• 제19권4호
• /
• pp.337-342
• /
• 1991

전국 162개 지역에서 수집한 토양에서 분리한 5,000여종의 토양 미생물 탐색을 통해서 항진균 활성이 우수한 한개의 생산균주를 선별하고 형태학적 배양학적, 생리학적인 균주 특성을 조사하고 Bacillus subtilis로 동정하였다. 이 균주가 생산하는 항진균물질은 벼도열병균, 회색곰팡이병균, 무좀균, 캔디다균 등에 효과가 있었으며 벼의 도열병에 대한 포장에서의 활성은 tricyclazole 보다는 낮지만 kasugamycin과 대등한 방제효과를 나타내었다. 한편 국소 시험에서는 자극성이 없는 것으로 나타났으며, 급성경구 독성은 5,000mg/kg 군에서 1마리가 사망하여 $LD_{50}$ 값은 5,000mg/kg 이상으로 추정되어 KRF-001은 저독성내지 무독성 항진균물질로 유추된다.

• 한국식품영양학회지
• /
• 제33권5호
• /
• pp.505-511
• /
• 2020

In this study, hot water and ethanol, acetone, and hexane extracts of the steamed Deodeok were investigated for use as raw materials for natural cosmetics. Hot water and ethanol, acetone and hexane extracts of steamed Deodeok did not indicate cell toxicity up to 300 ㎍/mL concentration in the MTT assay. The tyrosinase inhibition effects of the ethanol extract were higher than the ascorbic acid. The efficacy of the steamed Deodeok's ethanol extract on the elastase inhibition was similar to that of quercetin at higher concentrations. In conclusion, water, ethanol, acetone and hexane extracts of the steamed Deodeok indicated strong possibility as a whitening and anti-wrinkle functional cosmetic ingredient. Among them, the steamed Deodeok's ethanol extract, which showed excellent activity, is highly available because it has high safety compared to other organic solvent extraction methods used in cosmetics, and has minimal irritation to the skin.

• 농약과학회지
• /
• 제14권4호
• /
• pp.490-498
• /
• 2010

살균제 isotianol의 독성을 평가하고 일일섭취허용량을 설정하기 위하여 급성독성시험 등 시험성적서를 검토하였다. Isotianil은 소변과 대변을 통해 빠르게 대사되었다. 급성경구독성은 저독성이었으며 피부자극, 안점막자극성은 없는 것으로 나타났다. 피부감작성은 약한 감작성으로 판단되었으며, 유전독성은 없었다. 랫드와 개 만성독성시험 결과 간의 무게, 혈액학 및 임상생화학적 변화가 관찰되었다. 또한 생식독성과 기형독성에는 영향이 없었다. Isotianil은 12개월 만성독성시험의 최대무작용량이 2.83 mg/kg/bw/day로 가장 민감하였고, 안전계수 100을 적용하여 일일섭취허용량을 0.028 mg/kg/bw/day로 설정하였다.

• 폴리머
• /
• 제33권2호
• /
• pp.111-117
• /
• 2009

본 연구를 통해 역이온 영동(reverse iontophoresis)을 이용한 무채혈 혈당 측정시스템의 patch sensor에 사용되는 효소 고정형 수화젤의 생체적합성 여부를 확인하고자 하였다. 아크릴레이트 계열의 단량체를 사용하여 일정한 unit의 효소가 고정된 수화젤을 합성하였다. 합성된 수화젤의 물성분석을 위해 FT-IR spectrometer를 이용하여 구조분석을 하고 DSC를 이용하여 열적 안정성을 확인하였다. 또한, UV-Vis spoctrophotometer를 이용하여 표준품 대비 50% 이상의 효소 활성도를 확인하였다. 표면의 효소 고정화 확인을 위해 SEM을 이용하여 확인한 결과 효소가 고정화되어 있음을 확인하였다. 수화젤은 환자의 피부에 직접적으로 접촉됨으로써 접촉 시 무해성을 평가하기 위하여, ISO-10993에 의하여 세포독성(cytotoxicity), 피내반응(intracutaneous reactivity), 피부자극(skin irritation) 및 감작성(maximization sensitization) 시험을 실시하였고 이를 통해 생체적합성이 우수하다는 것을 확인하였다.

• Toxicological Research
• /
• 제16권4호
• /
• pp.302-309
• /
• 2000

The purpose of this study is to investigate toxic effects of iso-butylalcohol (iBA) in Sprague-Dawley (SD) rats under the exposure of 6 hours a day, 5 days a week for 13 weeks by inhalation, and to evaluate the occupational safety of iBA in comparison with the permissible exposure level (PEL) stipulated by the Occupational Safety and Health Administration (OSHA). iBA did not induce any abnormal changes from the aspects of clinical signs, feed consumption, ophthalmic test, urinalysis, hematology and blood chemistry during and at the terminal of the inhalation toxicity tests. We did not find any abnormal findings in the gross and microscopic observations due to the inhalation of iBA. There was no alteration in relative organ weights by the inhalation of iBA. No observed adverse effect level (NOAEL) of iBA was considered to be more than 3,000 ppm in rats under the inhalation of 6 hours a day, 5 days a week for 13 weeks. Fifty ppm of iBA, the PEL regulated by OSHA, is too conservative for working places. As iBA showed no abnormal observations in all the experimental parameters at any concentration under this experimental condition, we suggest that 150 ppm is safe enough for the PEL of iBA in the working areas, even taking into onsideration that OSHA lowered the PEL to 50 ppm for fear of the probable risk of its skin irritation.

• 대한화장품학회:학술대회논문집
• /
• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
• /
• pp.294-302
• /
• 2003

Unstable cosmetic active ingredients could be degraded rapidly by chemical and photochemical process. Particularly, some of active ingredients like retinol are known to cause skin irritation when applied on the skin excessively. Therefore, it has become a very important issue to encapsulate cosmetic actives for the stabilization and skin protection. This study was performed in order to prepare a chitosan microcapsule containing liposoluble cosmetic actives and to investigate the stabilization effect of actives when chitosan microcapsule was applied in cosmetic formulation. Chitosan, deacetylated form of chitin, has been of interest in the industrial applications due to its biocompatibility, biodegradability, non-toxicity, antimicrobial activity and also used as a wall material of capsule. Retinol was used as a core material and was stabilized by a wall of chitosan and antioxidants. The chitosan microcapsule containing retinol(CMR) was prepared by using coacervation method and W$_1$/O/W$_2$ emulsification techniques. The CMR has 0.5~10.0 ${\mu}{\textrm}{m}$ size distribution and a long-term stability of more than an year inside the cosmetic formulation(O/W). Remaining retinol percentages at 45$^{\circ}C$ after 8 weeks in the CMR dispersion were 15.6%(pH 4.0), 59.8%(pH 6.0) and 65.0%(pH 6.0 with antioxidant) respectively. Retinol stability when added CMR inside a ONV emulsion was better than that of ONV emulsion added non-capsulated retinol. As a result, remaining retinol at 45$^{\circ}C$ after 8 weeks in O/W emulsion added non-capsulated retinol and O/W emulsion containing CMR was 12.7%, 70.5% respectively. It appeared that chitosan treated microcapsule may be used for a potential encapsulation method of unstable active ingredients.