• Journal of the Korean Applied Science and Technology
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• v.41 no.1
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• pp.27-35
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• 2024

This study selected 21 domestic prior documents published in domestic KCI sites related to snowboarding after the 2018 Pyeongchang Winter Olympics for about five years from 2018 to 2023 and analyzed the characteristics, participation type, research method, and research division, The results are as follows. First, in terms of gender, papers on only men or men and women accounted for the majority, and the ages were distributed in various ways from 20s to 50s. In the case of the number of subjects, papers with less than 50 subjects accounted for the majority. Second, in the form of participation, registered player and ski resort users accounted for the majority, and papers were formed youth player, instructor, and judges in order. Third, Mostly, there are survey and experimental studies for the research methods, and qualitative and integrated studies were analyzed in order. Fourth, sports sociology and biomechanics were mainly used as research divisions, and sports psychology, physiology, sports measurement and evaluation, sports managment, and sports history were analyzed in order.

• Korean Journal of Environment and Ecology
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• v.34 no.1
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• pp.63-71
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• 2020

This study characterized the spatial and seasonal patterns of light pollution in the Deogyusan National Park and examined the potential effects of light pollution on ecosystems in the park using light intensities derived from VIIRS (Visible Infrared Imaging Radiometer Suite) DNB (Day and Night Band) nightlight images collected in January and August 2018. Results showed that the Muju Deogyusan resort had the greatest light intensity than other sources of light pollution in the park, and light intensity of the resort was much higher in January than in August, suggesting that artificial lights in ski slopes and facilities were the major source of light pollution in the park. An analysis of an urban-natural light pollution gradient along a neighboring urban area through the inside of the park indicated that light radiated from a light pollution source permeated for up to 1km into the adjacent area and contaminated the edge area of the park. Of the legally protected species whose distributions were reported in literature, four mammals (Martes flavigula, Mustela nivalis, Prionailurus bengalensis, Pteromys volans aluco), two birds (Falco subbuteo, Falco tinnunculus), and nine amphibians and reptiles (Onychodactylus koreanus, Hynobius leechii, Karsenia koreana, Rana dybowskii, Rana huanrenensis, Elaphe dione, Rhabdophis tigrinus, Gloydius ussuriensis, Gloydius saxatilis) inhabited light-polluted areas. Of those species inhabiting light-polluted areas, nocturnal species, such as Prionailurus bengalensis and Pteromys volans aluco, in particular, were vulnerable to light pollution. These results implied that protecting ecosystems from light pollution in national parks requires managing nighttime light in the parks and surrounding areas and making a plan to manage nighttime light pollution by taking into account ecological characteristics of wild animals in the parks.

• Korean Journal of Food Science and Technology
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• v.36 no.1
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• pp.141-146
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• 2004

Fungal products indirectly mediate anti-tumor effects in vitro and in vivo. To investigate whether Lentinus edodes might possess direct anti-tumor substance, L. edodes was extracted and tested on human papillomavirus (HPV) 16 oncogenes-associated animal tumor cells (TC-1) and in an animal tumor model. Only water extract displayed direct anti-proliferative effects in TC-1 tumor cells in vitro. This inhibition was dose-dependent, and inhibitory concentration ($IC_{50}$) was $800\;{\mu}g/mL$. Fungal extracts also showed growth inhibition to human cervical cancer cells (CaSki and HeLa) similarly to TC-1 tumor cells. When fungal extracts were added at a high dose (1.5 mg/mL), cell growth was inhibited within 6 hr following extract treatment. Cell growth inhibition was blocked by heat treatment, but not by low pH, which is indicative of heat sensitivity of this anti-proliferative substance. Cell growth suppression was mediated by apoptosis, as determined by Annexin V and propidium iodide staining. When challenged with TC-1 cells, direct intratumoral injection of fungal extracts resulted in some positive effect on tumor growth inhibition, as compared to oral delivery. Results suggest that heat labile substance of L. edodes suppresses growth of HPV oncogenes-associated tumor cells through apoptosis.

• Clinical and Experimental Pediatrics
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• v.46 no.12
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• pp.1186-1193
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• 2003

Purpose : Fragile sites are points on chromosomes which tend to break non-randomly when exposed to specific chemical agents or conditions of tissue culture. The chromosomal break induced by the antineoplastic drug, 1-${\beta}$-D-arabinofuranosyl-cytosine(Ara-c), was investigated to study the laboratory conditions in which the incidence of chromosomal break could be enhanced. Besides, the fragile sites induced by Ara-C were investigated and compared to the already known locations of the specific chromosomal alterations observed in specific neoplasms. Methods : T-lymphocytes from theree normal males and three females were cultured for 48 hours. Cells from each individual were exposed to the Ara-C for an additional 24 hours. After the caffeine was added during the last six hours culture, the metaphase chromosomes were prepared following the conventional method. A site was considered fragile if it was found to break two or more per 100 chromosomal breaks in more than four of six individuals tested. Results : Ara-C induced 252.1 chromosomal breaks per 100 mitotic cells and this result was significantly higher than that of the control, which induced 25.2 breaks(P<0.05). The incidence of the chromosomal break by Ara-C was higher, if cultured in the MEM-FA, which has no folic acid, than in the RPMI 1640 which contains enough folic acid(P<0.05). The most common break site by Ara-C was 3p14.2(FRA3B). There were 20 fragile sites induced by Ara-C. Among these 20 fragile sites, seven coincided with the locations of the mapped oncogenes, JUN, SKI, REL, N-MYC, FHIT, MET, ETS-1, and FOS. Conclusion : S phase specific chemotherapeutic agent, Ara-C, induced the expression of the chromosomal fragile sites effectively using the T-lymphocyte in vitro. Some of the fragile sites by Ara-C highly coincided with the oncogenes and neoplasm specific chromosome breakpoints. In this regard, the fragile sites reported here could provide the unknown neoplasm related chromosomal alternation points.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.3 no.1
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• pp.110-126
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• 1993

Worker exposures to total chromium, hexavalent chromium (VI), sulfuric acid and alkaline dust at electroplating operations and worker exposures to trichloroethylene (TCE) and methyl chloroform (MCM) at degreasing operations in eleven small industrial plants were evaluated. Appropriate local exhaust ventilation systems for both operations were designed and recommended. Results of the study are summarized as follows ; 1. Out of 134 measurements for airborne hexavalent chromium concentrations, seven were exceeding the Korean occupational health standard of $50{\mu}g/m^3$ and 45 were exceeding the NIOSH standard of $1{\mu}g/m^3$. With an exception of one measurement, concentrations of total chromium were below the Korean standard of $500{\mu}g/m^3$. 2. Worker exposures to chromium were closely related to the existing control methods at the electroplating operations. Local exhaust systems, partial coverage of the tank surface, and antifoaming agents on liquid surface were adopted as control methods. 3. With an exception of one sample, airborne concentrations of sulfuric acid and alkaline dusts were below the applicable occupational heatlth standards. 4. Three plants indicated that airborne concentrations of TCE and MCM were exceeding the Korean standards. Other plants showed lower concentrations than the standards. It should be noted that generally, the activities and workloads on the day of surveys were less than normal. 5. Since the most existing ventilation systems did not satisfy the ACGIH criteria, the ventilation systems should be improved. Some examples for designing appropriate ventilation systems are presented.

• YAKHAK HOEJI
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• v.48 no.6
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• pp.328-334
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• 2004

Cervical cancer is one of the leading causes of female death. Viral oncoproteins E6 and E7 are selectively retained and expressed in carcinoma cells infected with HPV (Human pa pilloma virus) type 16 and cooperated in immotalization and transformation of primary keratinocyte. E6 and E7 oncoproteins interfere the functions of tumor suppressor proteins p53 and retinoblasoma protein (pRb), respectively. Among a lots of natural products, Mentha arvensis Linne var.piperascens have inhibitory effects on bindings between E6 oncoprotein and tumor suppressor p53, E3 ubiqutin- protein ligase (E6AP). HPV oncoprotein inhibitors from Mentha piperita L. were isolated by solvent partition and column chromatography (Silica gel, RP-18) and inhibitory compounds were finally purified by HPLC using an ELISA screening system based on binding between E6 and E6AP. The aim of this study is to identify the structure of inhibitory compounds and to investigate whether these compounds have inhibitory effects on functions of E6 oncoprotein. We investigated whether caffeic acid methyl ester (CAM) extracted from Mentha piperita L. could inhibit the function of E6 oncoprotein. CAM inhibited the in vitro binding of E6 and E6AP which are essential for the binding and degradation of the tumor suppressor p53 and also inhibited the proliferation of human cervical cancer cell lines (SiHa and CaSKi) in a dose response manner. These results suggest that CAM inhibited the function of E6 oncoprotein, suggesting that it can be used as a potential drug for the treatment of cervical cancers infected with HPV.

• Korean Journal of Environment and Ecology
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• v.7 no.1
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• pp.22-28
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• 1993

Recently, by fast industralization and development without environmental concern, our environment deterioration have being continued rapidly. Therefore, many species is placed on crisis of extinction or was already extincted by habitat destruction. In Minjujisan area, there is a plan to construct the Sport and Leisure Complex by which convert the forest conservancy district to the district of development promotion and district of sighseeing and leisure by Yongdong District. To investigate the avifauna impacts by habitat destruction, bird censuses was carried out before the Sport and Leisure Complex be construct in Minjujisan and Sokgisan area during 4-5 May, 1992 and 16-21 June, 1992. Total 229 birds of 41 species in 23 family of 9 orders were recorded during censuses. Sitta europaea amurensis was the most abundant species and Parus ater amurensis, Phylloscopus borealis xanthodryas, Parus major wladiwostokensis, Parus palustris hellmayri, and Turdus pallidus were dominant species. Accipiter nisus nisosimilis, Accipiter soloenis, and Otus scops stictonotus were found out to the natural monuments in this suvey. Halcyon pileate was observed to a rare bird in this surveyed area. Species diversity of this census was 3.381 which showed slightly higher degree than other surveyed area. The area of Minjujisan, Sokgipong, and Samdopong which have little pollution agent around there and kept away from mankind were approved the heavy avifauna area which have so many bird species. Biogeographically, the Taebaek Mountains was connected with the Sobaek Mountains through this area, therefore it was significant that many animal species could be move to each mountains ranges. The construction plan of the Sport and Leisure Complex in Minjujisan area should be restrain with concern about environmentally destruction by the Muju Resort in Dokyusan National Park which connected to that area have master plan to development the ski slopes for the 1997 Universiad with permission by the Ministry of Environment.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.4
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• pp.1325-1331
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• 2012

Tumor formation and growth is dictated by a very small number of tumor cells, called cancer stem cells, which are capable of self-renewal. The genesis of cancer stem cells and their resistance to conventional chemotherapy and radiotherapy via mechanisms such as multidrug resistance, quiescence, enhanced DNA repair abilities and anti-apoptotic mechanisms, make it imperative to develop methods to identify and use these cells as diagnostic or therapeutic targets. Aldehyde dehydrogenase 1 (ALDH1) is used as a cancer stem cell marker. In this study, we evaluated ALDH1 expression in CaSki, HeLa and SiHa cervical cancer cells using the Aldefluor method to isolate ALDH1-positive cells. We showed that higher ALDH1 expression correlated with significantly higher rates of cell proliferation, microsphere formation and migration. We also could demonstrate that SiHa-ALDH1-positive cells were significantly more tumorigenic compared to SiHa-ALDH1-negative cells. Similarly, SiHa cells overexpressing ALDH1 were significantly more tumorigenic and showed higher rates of cell proliferation and migration compared to SiHa cells where ALDH1 expression was knocked down using a lentivirus vector. Our data suggested that ALDH1 is a marker of cervical cancer stem cells and expand our understanding of its functional role.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.10
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• pp.1525-1530
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• 2005

The purpose of this study is to investigate anti-tumor activities, general composition, elemental composition and mineral contents of fermented liquid stem, root and fruit of Opuntia humifusa. In the general composition, the energy, crude protein, crude lipid and crude carbohydrate contents of fermented liquid stem were 86.21 Kcal, 0.92$\%$, 0.12$\%$, and 20.34$\%$, respectively. Fermented liquid fruit showed 65.32 Kcal, 1.04$\%$, 0.08$\%$, and 15.15$\%$. In mineral analysis, fermented liquid stem and fruit showed 1,800 and 388 mg of calcium per 100g. The ferrous concentrations of fermented liquid stem and fruit were 21 and 10 mg per 100 g, respectively. Methanol, ethanol and water extracts of nonfermented liquid stem and fruit did not inhibit the proliferation in human cervical cancer cells (Caski, SiHa and HaCaT), but the fermented liquid fruit showed the inhibition of Proliferation with dose-response manner in Caski and SiHa cells, but not HaCaT. Therefore, it suggests that fermented cactus may be used as one of potential adjuvant for the treatment of cervical carcinomas.

• The Academic Congress of Korean Shoulder and Elbow Society
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• 1995.05a
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• pp.7-7
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• 1995

최근에 스키가 동계스포츠로 각광받으면서 스키인구가 폭발적으로 증가함에 따라 스키손상도 많이 발생하게 되어 스키손상은 스포츠외상에서 중요한 비중을 차지하게 되었다. 스키인구의 증가에 따라 스키손상에 대한 정확한 분석과 이에 대한 예방대책이 절실히 요구되며 이에 경희대학교 의과대학 정형외과학 교실에서는 1982년 12월부터 1995년 3월까지( 1986년과 1987년을 제외 ) 11년간 용평스키장내에 동계스포츠클리닉을 개설하여 상기 기간 중 내장한 총 스키인구 3,093,667명중 스키손상으로 동계스포츠클리닉에 내원한 7,172명의 환자$(0.23\%)$에 대하여 스키손상의 년도별 발생빈도, 스키손상의 특징 스키골절과 탈구의 발생빈도 및 특징 등에 대하여 11년 간의 변화추세를 분석하였다. 11년 간의 스키장의 내장객수는 1983년을 기준 (83,100)으로 하였을 때 1995년에 $977\%로 국부 큰 증가를 보였으며, 스키인구 1000명당 손상율은 1983년의 3.4에서 1995년의 1.4(평균 2.3)로 감소하는 추세를 보였다. 스키손상의 형태는 단순열상 및 타박상등을 제외하고 슬관절 인대손상이 $22.6\%로 가장 많이 발생하였으며, 조사기간동안 수치상의 의미 있는 변화는 없었다. 스키골절 및 탈구는 스키인구 1000명당 평균 0.35였으며, 1983년의 0.90으로부터 점차 감소하여 1995년에는 0.21에 이르렀다. 연령별 스키골절 및 탈구는 20세 이하에서 평균 $41.6\%로 전체적으로 성인에서 더 많이 발생하였으며, 이는 처음 3년 간 20세 이하 골절 및 탈구가 $70.5\%로 높은 발생빈도를 보였으나 1995년도의 20세 이하 골절 및 탈구가$30.3\%까지 감소하게되어 년도가 증가함에 따라 청장년 층에 비하여 소아골절 및 탈구가 전체적으로 감소하는 경향을 보였다. 스키골절의 부위별 발생빈도는 1990년 이전까지 하지골절 및 탈구가 많았으나 이후 점차 상지의 골절 탈구가 증가하였다 하지에서 가장 많은 골절은 경골 골절이었으며, 경골골절은 회전력에 의한 나선형골절이 $76.5\%로 가장 많았고 년도에 따른 변화는 보이지 않았다. 스키손상의 발생빈도는 초기에 비하여 점차 감소하는 경향을 보였으며, 손상의 특성도 부위별, 연령별로 다양한 변화를 나타내었다.