• KSBB Journal
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• v.31 no.4
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• pp.300-311
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• 2016

Recently gene delivery has been designed newly using bioactive biomaterial and applied in the various field by many researchers. In this study, we proposed a new gene delivery system which has the capability of targeting effect in the specific tissue and remarkably enhanced transfection efficiency. We investigated $^1H-NMR$ spectroscopy, particle size analyzer and gel retardation to confirm the correct preparation of gene delivery. Also, we identified the hemo-compatibility of gene delivery by hemolysis assay, non-cytotoxicity by MTT test and transfection efficiency. The uptake mechanism of the gene carrier was confirmed using inhibitor agent such as sodium azide, indomethacin, quercetin, colchicine, and chloropromazine. As a results, it was identified that gene carrier prepared by in this study entered in the cell by the microtubule-dependent, energy-dependent and clathrin-mediated endocytosis pathway.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.130-131
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• 2013

Nanostructured oxides are widely used in heterogeneous catalysis where their catalytic properties are closely associated with the size and morphology at nanometer level. The effect of particle size has been well decumented in the past two decades, but the shape of the nanoparticles has rarely been concerned. Here we illustrate that the redox and acidic-basic properties of oxides are largely dependent on their shapes by taking $Co_3O_4$, $Fe_2O_3$, $CeO_2$ and $La_2O_3$ nanorods as typical examples. The catalytic activities of these rod-shaped oxides are mainly governed by the nature of the exposed crystal planes. For instance, the predominant presence of {110} planes which are rich in active $Co^{3+}$ on $Co_3O_4$ nanorods led to a much higher activity for CO oxidation than the nanoparticles that mainly exposed the {111} planes. The simultaneous exposure of iron and oxygen ions on the surface of $Fe_2O_3$ nanorods have significantly enhanced the adsorption and activation of NO and thereby promoted the efficiency of DeNOx process. Moreover, the exposed surface planes of these rod-shaped oxides mediated the reaction performance of the integrated metal-oxide catalysts. Au/$CeO_2$ catalysts exhibited outstanding stability under water-gas shift conditions owing to the strong bonding of gold particle on the $CeO_2$ nanorods where the formed gold-ceria interface was resistant towards sintering. Cu nanoparticles dispersed on $La_2O_3$ nanorods efficiently catalyzed transfer dehydrogenation of primary aliphatic alcohols based on the uniue role of the exposed {110} planes on the support. Morphology control at nanometer level allows preferential exposure of the catalytically active sites, providing a new stragegy for the design of highly efficient nanostructured catalysts.

• Environmental Analysis Health and Toxicology
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• v.16 no.2
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• pp.97-102
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• 2001

The flavin-containing monooxygenases(FMOs) (EC1.14.13.8) are NADPH0dependent flavoenzymes that catalyze oxidation of soft nucleophilic heteroatom centers in a range of structurally diverse compounds, including foods, drugs, pesticides, and other xenobiotics. In humans, FMO3 is quantitatively a major human liver monooxygenase. In the present study, the baculovirus expression vector system was used to overexpress human FMO3 in sect cells for stalytic studies. Microsomes isolated from Spodoptera frugiperda(Sf)9 cells infected with human FMO3 recombinant baculovirus catalyzed the NADPH-and O$_2$-dependent oxidation of methimazole, thiourea, and phenylthiourea. However there was no detectable activity with 1, 3-diphenylthiourea or larger thiocarbamides. Microsomes from control Sf9 cells were devoid of methimazole or thiourea S-oxygenase activity. 1, 3-diphenylthiourea is apparently completely excluded from the catalytic site, these amines drugs are probably approaching the upper size limits of xenobiotics accepted by human FMO3. The substrate specificity of this iosform in humans appears considerably more restriceted than that of pig, guinea pig, rat or rabbit FMO3.

• Journal of Aquaculture
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• v.11 no.1
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• pp.59-66
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• 1998

The effectiveness of lidocaine HCI (lidocaine HCI/sodium bicarbonate mixture) was tested as an anaesthetic for Rhynchocypris oxycephalus and R. steindachneri at three different temperatures of $10^{\circ}C.$, $15^{\circ}C.$ and $20^{\circ}C.$. Based on the exposure and recovery time, effective doses of lidocaine HCI were proven to be 300ppm ($20^{\circ}C.$), 400ppm ($15^{\circ}C.$), and 600ppm($10^{\circ}C.$) for R. oxcephaus, and 400ppm ($20^{\circ}C.$), 500ppm ($15^{\circ}C.$), and 600ppm ($10^{\circ}C.$) for R. steindachneri respectively. Anaesthetic dose and temperature-dependent relationship in exposure and recovery time were obseved for these two Rhynchocypris spops. There were size-related increases of exposure time on R. steindachneri in each dose of lidocaine HCI. However, dost-dependent increase of recovery time wea found in only the large size group of Rhynchocypris spp.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.44 no.12
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• pp.1027-1034
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• 2016

In this research, experimental study about size effect of solid particles on erosion resistance is presented. A high-density polyethylene particle with a mm-sized diameter is accelerated using a two-stage light gas gun up to Mach number of approximately 3.0. An accelerated particle impacts aluminum alloy such as Al1050 and Al6061 T6, and infrared windows such as ZnS and sapphire specimens. For the aluminum alloy, craters that form on the surface of the specimens are measured to characterize the erosion resistance of the material. For the infrared windows, repetitive tests are conducted until a linear or circumferential crack is found to create damage threshold curves that define a material's erosive resistance. From the comparison of test data for various sizes of high-density polyethylene particles, it is found that erosion resistance of material is linearly dependent on the size of particles.

• Journal of the Korean Geotechnical Society
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• v.27 no.6
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• pp.49-61
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• 2011

This paper presents the applicability of rheological models for describing fine-laden debris flows and analyzes the flow characteristics as a function of grain size. Two types of soil samples were used: (1) clayey soils - Mediterranean Sea clays and (2) silty soils - iron ore tailings from Newfoundland, Canada. Clayey soil samples show a typical shear thinning behavior but silty soil samples exhibit the transition from shear thinning to the Bingham fluid as shear rate is increased. It may be due to the fact that the determination of yield stress and plastic viscosity is strongly dependent upon interstructrual interaction and strength evolution between soil particles. So grain size effect produces different flow curves. For modeling debris flows that are mainly composed of fine-grained sediments (<0.075 mm), we need the yield stress and plastic viscosity to mimic the flow patterns like shape of deposition, thickness, length of debris flow, and so on. These values correlate with the liquidity index. Thus one can estimate the debris flow mobility if one can measure the physical properties.

• Particle and aerosol research
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• v.4 no.1
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• pp.9-20
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• 2008

The effect of light intensity on the ozone formation and the aerosol number concentration during the photochemical reactions of ambient air was investigated in an indoor smog chamber. The smog chamber consists of a housing, 64 blacklights, and a $2.5-m^3$ reaction bag made of Teflon film. The bag was filled with the unfiltered ambient air in Seoul from January 10 to March 18, 2002. In this work, the photolysis rate of $NO_2$, $k_1$ was used as an index of light intensity. Three levels of light intensity were controlled by changing the number of blacklights turned on among 64 blacklights: $0.29min^{-1}$ (50%), $0.44min^{-1}$ (75%), $0.57min^{-1}$ (100%). The ozone concentration increased rapidly within 10 minutes after irradiation irrespective of light intensity, thereafter it increased linearly during the irradiation. The ozone production rate seems to be dependent on both the light intensity and the quality of ambient air introduced into the reaction bag. The change in aerosol number concentration also depended on both the light intensity and the ambient air quality, especially aerosol size distribution. Based on the initial ambient aerosol size distributions, the photochemical potential for aerosol formation and growth is classified into two cases. One is the case showing aerosol formation and growth processes, and the other is the case showing no apparent change in particle size distribution.

• Korean Journal of Social Welfare
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• v.69 no.1
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• pp.223-254
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• 2017

This study was carried out to examine whether the moderated mediating effect of SNS addiction resulting from marital dissatisfaction and loneliness is influenced by gender or size and composition of social capital. 714 married men and women in their 20's to 40's were participated in this study. The data were collected by online research center with quota sampling based on demographical proportion. To analyze this research model, the moderated mediating effect utilizing structural equation was used. The results of this study were summarized as follows. First, it is verified that the loneliness moderated between marital dissatisfaction and SNS addiction. Since the relationship between independent and dependent variable is insignificant, comparison of competition model was able to determine if mediating effect was fully or partially shown. As a result, the complete mediated model was ultimately chosen because its various goodness-of-fit index including $x^2$ performed fairly well. Second, the mediating effect which moderates social capital size was presented in the mediated model between the marital dissatisfaction, loneliness and SNS addiction. Specifically, It implies that group who recognizes their social capital big recognizes the route and effect size more than that who considers their social capital small. And the moderated mediating effect depending on different type of social capital among the marital dissatisfaction and loneliness and between SNS addiction was substantiated. It implies that disequilibration of SNS social capital in face-to-face sphere and in online acted as a danger fact with respect to the marital dissatisfaction, loneliness and SNS addiction. The implications of these findings were discussed, and directions for future studies were also proposed.

• Korean Journal of Environmental Biology
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• v.27 no.4
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• pp.334-340
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• 2009

In other to know the feeding preference over the degree of ripeness and size of fruits in birds, we gave them to three bird species in breeding condition, counted and analyzed the number of fruits by correspondence ordination technique. Chrysolophus pictus did not show a distinct preference in respect to degree of maturity or size of fruits of among three tree species. Columba rupestris preferred un-ripen fruits over ripen ones of Prunus yedoensis or Elaeagnus multiflora, but did not choose selectively in case of fruits of Malus sieboldii. Pavo muticus showed the reverse favorable pattern of Columba rupestris. Total feeding amounts eaten by birds were dependent on the initial feeding amounts of fruits. These results indicated that in feeding activity some birds chose differently in terms of species or maturity of fruits, but not in fruit size.

• Journal of Pharmaceutical Investigation
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• v.26 no.4
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• pp.245-256
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• 1996

Solid lipid nanoparticles (SLN) have been developed as a new drug delivery system. Although many particulate drug carriers, such as microsphere, liposome, niosome, emulsion, etc. have been introduced, they have some disadvantage; low efficiency of incorporation and stability, lack of reproducibility, and so on. Meanwhile, SLN as a new drug delivery system is known to entrap rugs with a high efficiency and a good reproducibility. Moreover, small size SLN can circulate in blood for a prolonged time. Although many preparation methods were introduced, microfluidization method is recommended to be the most useful. This study was attempted to prepare and evaluate ketoprofen-incorporated SLNs (keto-SLN), which were prepared by two methods, ultrasonication and microfluidization. Keto-SLN was evaluated by measurement of particle size and zeta potential, efficacy of entrapment, sedimentation volume, in virto release pattern. The mean particle size was about $0.1\;{\mu}m$, and the size was dependent on the type and the amount of emulsifier. Zeta potential was negative, $-9{\sim}-13mV$ and entrapment efficacy was very high and stability was good for at least 60 days in the respect of particle size and sedimentation volume ratio. Analgesic effect was also determined as well as pharmacokinetic parameters. The former was comparable to that of that of ketoprofen loaded suspension (keto-sus) and the latter revealed that consistent with the delayed release of keto-SLN. $T_{max}$ was longer than keto-sus. Therefore, keto-SLN was favourable dosage forms in the field of drug delivery system such as anti-cancer, analgesics and anti-inflammatory agents.