• Ocean Systems Engineering
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• v.9 no.1
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• pp.43-77
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• 2019

A local-analytic-based Navier-Stokes solver has been employed in conjunction with a compound ocean structure motion analysis program for time-domain simulation of passing ship effects induced by multiple post-Panamax class ships in the exact condition of a real waterway. The exact seabed bathymetry was reproduced to the utmost precision attainable using the NOAA geophysical database for Virginia Beach, NOAA nautical charts for Hampton Roads and Norfolk harbor, and echo sounding data for the navigation channel and waterfront facilities. A parametric study consists of 112 simulation cases with various combinations of ship lanes, ship speeds, ship heading (inbound or outbound), channel depths, drift angles, and passing ship coupling (in head-on or overtaking encounters) were carried out for two waterfront facilities at NAVSTA Norfolk and Craney Island Fuel Terminal. The present paper provides detailed parametric study results at both locations to investigate the site-specific passing ship effects on the motion responses of ships moored at nearby piers.

• Journal of the Korean Society of Safety
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• v.25 no.5
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• pp.62-68
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• 2010

The main purpose of this study was to examine relative effects on safety behaviors between global feedback and specific feedback at a construction site. Participants were 30 full-time workers at a construction site. Five safety related behaviors were identified as critical behaviors. An ABCB within-group design was adopted. After baseline (A), participants received global feedback on their average percentage of all five target safety behaviors (B). In the next phase, participants received specific feedback on the percentages of individual target behaviors (C). In the final phase, the global feedback condition was reintroduced (B). Results showed that both global feedback and specific feedback were effective in increasing the frequencies of safety behaviors and there were no differences in the effectiveness between the two types of feedbacks.

• Proceedings of the Microbiological Society of Korea Conference
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• 2002.10a
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• pp.159-161
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• 2002

Bacteriophage lambda integrase carries out the site-specific recombination of lambda. Integrase contains two DNA binding domains with distinct sequence specificity, namely arm-type binding and core-type binding domains. The amino-terminal arm-binding domain is structurally related to the three-stranded $\beta-sheet$ family of DNA-binding domains. Integrase binding to the high affinity arm-type site by the amino-terminal domain facilitates Int binding to the low affinity core-type site, where the cleavage and strand exchange occurs. The amino-terminal domain of Int also modulates the core-binding and catalysis through intramolecular domain-domain interaction and/or intermolecular interactions between Int monomers. In addition, the amino-terminal domain interacts cooperatively with excisionase during excision. This indicates that amino-terminal domain of Int plays an important role in formation of proper higher-order nucleoprotein structure required for lambda site-specific recombination.

• Journal of the Korean Society of Hazard Mitigation
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• v.7 no.5
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• pp.139-150
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• 2007

As computer technology has been rapidly advanced, geographic information system (GIS) is recently used in many disciplines. In this study, for a model area in Seoul, seismic hazard potential relating to site effects, which are influenced by the subsurface geotechnical conditions, was estimated using the GIS tool. The distribution of pre-existing borehole drilling data in Seoul metropolitan area was examined for the regional estimation of site-specific seismic responses at the model area. Spatial geo-layers across the entire model area were predicted by constructing a GIS-based geotechnical information system (GTIS). A microzonation of site period $(T_G)$ for estimating site-specific seismic responses at the model area was performed within the GTIS. The spatial microzoning map of $T_G$ indicated seismic vulnerability of two- to four-storied buildings in the model area. Furthermore, a site classification map for determining the design ground motion was established based on the $T_G$ within the GTIS. This informed that most of location in the model area was categorized into current site classes C and D. This seismic microzonation framework for the model area could be applicable particularly in the entire Seoul metropolitan area based on the pre-existing borehole data.

• Bulletin of the Korean Chemical Society
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• v.32 no.12
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• pp.4427-4430
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• 2011

• Bulletin of the Korean Chemical Society
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• v.28 no.6
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• pp.907-908
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• 2007

• BMB Reports
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• v.31 no.4
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• pp.399-404
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• 1998

In order to gain further insight on the relationship between structure and function of glutathione S-transferase (GST), the six active-site mutants, R13T, K44T, Q51A, Q64A, S65A, and D98A, of human GST P1-1 were expressed in Escherichia coli and purified to electrophoretic homogeneity by affinity chromatography on immobilized GSH. The active-site mutants showed marked differences in substrate specificity. The substitution of Gln51 with threonine resulted in a drastic decrease in the specific activities to <10% of the wild-type value. The substitution of Arg13 with threonine resulted in more decreased specific activity toward cumene hydroperoxide and in the $I_{50}$ values of S-(2,4-dinitrophenyl) glutathione and benanstatin A. These results suggest that the substitution of Arg13 with threonine changes the conformation of the active site to increase the affinity for the product or electrophilic substrate. Lys44 seems to be in the vicinity of the H-site of hGST P1-1 or may contribute to some extents to the electrophile binding.

• Journal of Microbiology and Biotechnology
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• v.19 no.3
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• pp.217-228
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• 2009

Mobile genetic segments, or transposons, are also referred to as jumping genes as they can shift from one position in the genome to another, thus inducing a chromosomal mutation. According to the target site-specificity of the transposon during a transposition event, the result is either the insertion of a gene of interest at a specific chromosomal site, or the creation of knockout mutants. The former situation includes the integration of conjugative transposons via site-specific recombination, several transposons preferring a target site of a conserved AT-rich sequence, and Tn7 being site-specifically inserted at attTn7, the downstream of the essential glmS gene. The latter situation is exploited for random mutagenesis in many prokaryotes, including IS (insertion sequence) elements, mariner, Mu, Tn3 derivatives (Tn4430 and Tn917), Tn5, modified Tn7, Tn10, Tn552, and Ty1, enabling a variety of genetic manipulations. Randomly inserted transposons have been previously employed for a variety of applications such as genetic footprinting, gene transcriptional and translational fusion, signature-tagged mutagenesis (STM), DNA or cDNA sequencing, transposon site hybridization (TraSH), and scanning linker mutagenesis (SLM). Therefore, transposon-mediated genetic engineering is a valuable discipline for the study of bacterial physiology and pathogenesis in living hosts.

• Journal of the Earthquake Engineering Society of Korea
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• v.4 no.2
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• pp.37-46
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• 2000

본 논문에서는 부지특성을 고려한 설계지반운동의 산정방법을 연구하였으며 그 해석결과를 지반 구조물의 내진설계에 적용하는 방법을 제안하였다 지진응답 해석시 사용되는 설계응답스펙트럼과 설계시간 이력등의 입력운동의 통제점 위치가 지반구조물 내진설동 지층내 암반운동 그리고 노두운동을 사용하는 방법으로 나눌 수 있고 이에 따라 작용 설계지진운동이 변화하므로 지반구조물의 경계조건에 적합한 방법을 사용하여야 한다.

• International conference on construction engineering and project management
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• 2015.10a
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• pp.109-110
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• 2015

This paper presents a computational method that identifies an exact set of optimal overlap rates between critical activities to meet job site specific needs by using rework cost-slope. The procedures to compute the exact solution are provided in peudocode algorithm. The method is coded into Exact Concurrent Construction Scheduling system that allows practitioners to make more informed decision in accordance with the site-specific condition involved in the overlapping of critical activities. Test cases verify the validity of the computational method and the usability of the system.