• Molecules and Cells
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• v.43 no.11
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• pp.909-920
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• 2020

Cytosolic Ca²⁺ levels ([Ca²⁺]_c) change dynamically in response to inducers, repressors, and physiological conditions, and aberrant [Ca²⁺]_c concentration regulation is associated with cancer, heart failure, and diabetes. Therefore, [Ca²⁺]_c is considered as a good indicator of physiological and pathological cellular responses, and is a crucial biomarker for drug discovery. A genetically encoded calcium indicator (GECI) was recently developed to measure [Ca²⁺]_c in single cells and animal models. GECI have some advantages over chemically synthesized indicators, although they also have some drawbacks such as poor signal-to-noise ratio (SNR), low positive signal, delayed response, artifactual responses due to protein overexpression, and expensive detection equipment. Here, we developed an indicator based on interactions between Ca²⁺-loaded calmodulin and target proteins, and generated an innovative GECI sensor using split nano-luciferase (Nluc) fragments to detect changes in [Ca²⁺]_c. Stimulation-dependent luciferase activities were optimized by combining large and small subunits of Nluc binary technology (NanoBiT, LgBiT:SmBiT) fusion proteins and regulating the receptor expression levels. We constructed the binary [Ca²⁺]_c sensors using a multicistronic expression system in a single vector linked via the internal ribosome entry site (IRES), and examined the detection efficiencies. Promoter optimization studies indicated that promoter-dependent protein expression levels were crucial to optimize SNR and sensitivity. This novel [Ca²⁺]_c assay has high SNR and sensitivity, is easy to use, suitable for high-throughput assays, and may be useful to detect [Ca²⁺]_c in single cells and animal models.

• Korean Journal of Optics and Photonics
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• v.28 no.2
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• pp.75-82
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• 2017

We present a new type of receiver-transmitter optical system that can be adapted to the sensor head of a displacement-measuring interferometer. The interferometer is utilized to control positioning error and repetition accuracy of a wafer, down to the order of 1 nm, in a semiconductor manufacturing process. Currently, according to the tendency of scale-up of wafers, an interferometer is demanded to measure a wider range of displacement. To solve this technical problem, we suggest a new type of receiver-transmitter optical system consisting of a GRIN lens-Collimating lens-Afocal lens system, compared to conventional receiver-transmitter using a single collimating lens. By adapting this new technological optical structure, we can improve coupling efficiency up to about 100 times that of a single conventional collimating lens.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.66 no.7
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• pp.1149-1154
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• 2017

In this paper, we propose a sensorless multi-function controller applicable for deep well water pumps using current detection method. The proposed system overcomes various drawbacks of existing sensored system and additional features like Over current protection function due to overload, Under current protection function for idling at low water level and Relay function for starting single phase motors and acts as a level indicator to detect water lever in real time by the current detection method. A prototype of the multi-function controller system is designed and all of its functions are tested in the laboratory. The application of the proposed controller ensures reduction in the power consumption and maintenance cost in the facilities like water and septic tanks, drainage and waste water system, oil and chemical tanks where deep well pumps are used.

• Proceedings of the KSME Conference
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• 2004.11a
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• pp.696-701
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• 2004

A vehicle cruise control algorithm using an Interacting Multiple Model (IMM)-based Multi-Target Tracking (MTT) method has been presented in this paper. The vehicle cruise control algorithm consists of three parts; track estimator using IMM-Probabilistic Data Association Filter (PDAF), a primary target vehicle determination algorithm and a single-target adaptive cruise control algorithm. Three motion models; uniform motion, lane-change motion and acceleration motion, have been adopted to distinguish large lateral motions from longitudinal motions. The models have been validated using simulated and experimental data. The improvement in the state estimation performance when using three models is verified in target tracking simulations. The performance and safety benefits of a multi-model-based MTT-ACC system is investigated via simulations using real driving radar sensor data. These simulations show system response that is more realistic and reflective of actual human driving behavior.

• Proceedings of the KIEE Conference
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• 2011.07a
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• pp.2053-2054
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• 2011

Cardiopulmonary resuscitation(CPR) is performed by artificial ventilation and thoracic compression for the patient under emergent situation to maintain at least the minimum level of respiration and blood circulation for life survival. Good quality CPR requires monitoring respiration. We developed a system for continuous monitoring respirational signal while CPR, using respirational airflow sensor for CPR. Signal extraction circuit obtains pressure signal while CPR. Obtained signal would be performed analog-digital conversion and changed to airflow value by characteristic formula. Single inspiration and expiration were considered a period. Detected valid data were displayed LCD.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1993.04b
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• pp.218-222
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• 1993

A sliding mode controller associated with the moving sliding surface is formulate for the tip position control of a single-link flexible manipulator. After establishing the plant model which characterizes a noncollocated control system, a discontinuous control law is then constructed by restricting that velocity state variables are not available from direct sensor measurements. Using the proposed control law favorable system responses are accomplished through shortening the reaching phase without increasing maximum control torque. Furthermore, a low sensitiveness to extraneous disturbance is obtained. Computer simulations are undertaken in order to demonstrate these superior control performance characteristics to be accrued from the proposed methodology.

• International Journal of Aeronautical and Space Sciences
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• v.8 no.1
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• pp.105-114
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• 2007

This article presents the details of a designed control moment gyroscope (CMG) with a constant speed momentum wheel and one-axis-gimbal, and its experimental results performed at Korea Aerospace Research Institute. The CMG which is able to produce a torque of lOO mNm per each, is mounted in a pyramid configuration with four SGCMGs. Each CMG test and a single axis maneuver test with four-CMG cluster configuration are performed to confirm their performance on a ground-test facilities consisted of three major parts: a vibration isolation system, a dynamic force plate (Kistler sensor), and a DSP board. These facilities provide the accurate data of three axial and torques from the control moment gyro. Details of the CMG experimental results are presented with discussion of the experimental errors. The experimental data are compared with theoretical results and both results are used to verify their performance specifications.

• Transactions on Control, Automation and Systems Engineering
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• v.4 no.4
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• pp.270-276
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• 2002

An integrated robust fault diagnosis and fault accommodation strategy for a class of linear stochastic systems subjected to unknown disturbances is presented under the assumption that only a single fault may occur at a given time. The strategy is based on the fault isolation and estimation using a bank of robust two-stage Kalman filters and introduction of the additive compensation input for cancelling out the fault's effect on the system. Each filter is set up such that the residual is decoupled from unknown disturbances and fault with the influence vector designed in the filter. Simulation results for the simplified longitudinal flight control system with parameter uncertainties, process and sensor noises demonstrate the effectiveness of the present approach.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1996.11a
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• pp.1067-1072
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• 1996

Sensitivity analysis of the cylindrical zigzag cams under acceleration and centrifugal forces is performed. A Lagrangian method is used to determine the mechanism constant of zigzag track, And the equation of motion for cylindrical zigzag cam under rectangular pulse is derived by the governing equations of a single spring mass system. The ratio of the drive force tn resisting force is derived by angular acceleration, centrifugal force and setback force on the operation of the munition. The theoretical sensitivity curves for 3 models are analyzed. And experiments for 3 models are conducted to check safe and functional zone. Zigzag cam types can be satisfied all major design requirements for switch system of munition.

• Journal of the Optical Society of Korea
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• v.19 no.5
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• pp.531-536
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• 2015

This paper presents a new graphical method for selecting a pair of optical glass and housing materials to simultaneously achromatize and athermalize a multilens system composed of many elements. To take into account the lens spacing and housing, we quantify the lens power, chromatic power, and thermal power by weighting the ratio of the paraxial ray height at each lens to them. In addition, we introduce the equivalent single lens and the expanded athermal glass map including a housing material. Even though a lens system is composed of many elements, we can simply identify a pair of glass and housing materials that satisfies the athermal and achromatic conditions. Applying this method to design a black box camera lens equipped with a 1/4-inch image sensor having a pixel width of $2{\mu}m$, the chromatic and thermal defocusings are reduced to less than the depth of focus, over the specified ranges in temperature and frequency.