• Journal of Power System Engineering
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• v.3 no.4
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• pp.69-78
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• 1999

In the single-input and single-output system, the parameter of plant is scalar polynomial, but in the multiple input and multiple output, it accompanies, being matrix polynomial, the consideration of observable controlability index or problems non-commutation in matrix polynomial as well as degree, and it is more complex to deal with. Therefore, it is thought that a full research on the single-input and single-output system is not sufficient. This paper proposes that problems of minimum variance self-tuning regulator by using numerical calculation example of multivariable system and pole assignment self-tuning regulator.

• Computers and Concrete
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• v.19 no.2
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• pp.211-216
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• 2017

Crack on concrete surface allows more rapid penetration of chlorides. Crack width and depth are dominant parameters for chloride behavior, however their effects on chloride penetration are difficult to quantify. In the present work, the previous anisotropic (1-D) model on chloride diffusion in concrete with single crack is improved considering crack shape and roughness. In the previous model, parallel-piped shape was adopted for crack shape in steady-state condition. The previous model with single crack is improved considering wedge shape of crack profile and roughness. For verifying the proposed model, concrete samples for nuclear power plant are prepared and various crack widths are induced 0.0 to 1.2 mm. The chloride diffusion coefficients in steady-state condition are evaluated and compared with simulation results. The proposed model which can handle crack shape and roughness factor is evaluated to decrease chloride diffusion and can provide more reasonable results due to reduced area of crack profile. The roughness effect on diffusion is evaluated to be 10-20% of reduction in chloride diffusion.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.3 no.1
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• pp.10-19
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• 2002

The purpose of this paper is to describe the design of a general multipurpose batch process or plant in terms of a series of mathematical programing models, and to develop approach solution methodologies. The proposed model for a single period is based on the formulation (MINLP; Mixed Integer Nonlinear Programming) of Papageorgaki and Reklaitis [1], but was linearized (MILP; Mixed Integer Linear Programming) so as to obtain an exact and practical solution, and to allow treatment of uncertainties to be considered in expanding the plant. As a solution strategy a modified Benders' Decomposition was introduced and was tested on three example problems. The optimizing solver, OSL code provided by the IBM Corporation, was used for solving the problems. The solution method was successful in that it showed remarkable reduction in the computing times as compared with the direct solution method.

• The Plant Pathology Journal
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• v.26 no.4
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• pp.409-416
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• 2010

Fusarium species, a large group of plant pathogens, potentially pose quarantine concerns worldwide. Here, we focus on the development of a method for detecting four Fusarium species in quarantined plants in Korea: F. solani f. sp. cucurbitae, F. stilboides, F. redolens, and F. semitectum var. majus. Species-specific primers were designed from the nucleotide sequences of either the translation elongation factor-1 alpha (TEF1) gene or RNA polymerase II subunit (RPB2) gene. Two different primer sets derived from TEF1, all specific to F. solani f. sp. cucurbitae, were able to differentiate the two races (1 and 2) of this species. A set of nested primers for each race was designed to confirm the PCR results. Similarly, two primer sets derived from RPB2 successfully amplified specific fragments from five F. stilboides isolates grouped within a single phylogenetic clade. A specific TEF1 primer set amplified a DNA fragment from only four of the 12 F. redolens strains examined, which were grouped within a single phylogenetic clade. All of the F. semitectum var. majus isolates could be specifically detected with a single RPB2 primer set. The specificity of the primer sets developed here was confirmed using a total of 130 Fusarium isolates.

• The Plant Pathology Journal
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• v.32 no.6
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• pp.500-507
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• 2016

Single-molecule real-time (SMRT) sequencing allows identification of methylated DNA bases and methylation patterns/motifs at the genome level. Using SMRT sequencing, diverse bacterial methylomes including those of Helicobacter pylori, Lactobacillus spp., and Escherichia coli have been determined, and previously unreported DNA methylation motifs have been identified. However, the methylomes of Xanthomonas species, which belong to the most important plant pathogenic bacterial genus, have not been documented. Here, we report the methylomes of Xanthomonas axonopodis pv. glycines (Xag) strain 8ra and X. campestris pv. vesicatoria (Xcv) strain 85-10. We identified $N^6$-methyladenine (6mA) and $N^4$-methylcytosine (4mC) modification in both genomes. In addition, we assigned putative DNA methylation motifs including previously unreported methylation motifs via REBASE and MotifMaker, and compared methylation patterns in both species. Although Xag and Xcv belong to the same genus, their methylation patterns were dramatically different. The number of 4mC DNA bases in Xag (66,682) was significantly higher (29 fold) than in Xcv (2,321). In contrast, the number of 6mA DNA bases (4,147) in Xag was comparable to the number in Xcv (5,491). Strikingly, there were no common or shared motifs in the 10 most frequently methylated motifs of both strains, indicating they possess unique species- or strain-specific methylation motifs. Among the 20 most frequent motifs from both strains, for 9 motifs at least 1% of the methylated bases were located in putative promoter regions. Methylome analysis by SMRT sequencing technology is the first step toward understanding the biology and functions of DNA methylation in this genus.

• Research in Plant Disease
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• v.14 no.2
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• pp.102-106
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• 2008

The single application of Bacillus subtilis QST713(BS QST713) mixed application with fungicides and alternate application were treated to examine the control of alternaria leaf blight of Panax ginseng. Control value of alternaria leaf blight by single application of BS QST713 at 10 days interval was 83.3%, and those of single application at 10 days interval was $80.4{\sim}83.7%$ by azoxystrobin, chlorothalonil copper sulfate basic, copper sulfate basic, kresoxim-methyl, difenoconazole, mancozeb. When mixture of BS QST713 and fungicides were applied at 14 days interval, the control value of alternaria leaf blight were 83.6% by BS QST713 and mancozeb, and 82.6% by BS QST713 and azoxystrobin. However, mixture of BS QST713 with difenoconazole, kresoxim-methyl, copper sulfate basic and chlorothalonil copper sulfate basic exhibited the disease control values from 61.1% to 76.4%, which showed slightly lower control efficacy. In Daejeon, the alternate application of BS QST713 followed by copper sulfate basic with 14 days interval was 85.9% in control value, which showed the best control efficacy. The alternate application with other tested fungicides slightly decreased to $55.5{\sim}78.2%$ in control value. However, the alternate application of BS QST713 followed by fungicides showed very high control efficacy, which were approximately 90% in Muju, Jeonbuk Province. Consequently, the single, mixed or alternate application of BS QST713 and fungicides could be recommended as a control method to reduce the amount of fungicides.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.71-71
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• 2019

The genus Cenchrus (Poaceae), containing ca. 23 species, is distributed throughout Australia, Africa, Indian sub-continent, and America. In Korea, Cenchrus longispinus (Hack.) Fernald, especially introduced to Daecheong Island in 1999, is one of the most hazardous invasive plant which causes serious environmental threats, biodiversity damages and physically negative impact on humans and animals. Based on the next-generation sequencing (NGS) technology, we characterized the chloroplast (cp) genome sequences of C. longispinus which contains a large single copy (LSC; 80,223 bp), a small single copy (SSC; 12,449 bp), separated by a pair of inverted repeats (IRs; 22,236 bp). Additionally, we analyzed the cp genome sequences of Cenchrus echinatus L. which contains a large single copy (LSC; 80,220 bp), a small single copy (SSC; 12,439 bp), separated by a pair of inverted repeats (IRs; 22,236 bp). These cp genomes consist of 75 unique genes, 4 rRNA coding genes, 33 tRNA coding genes and 21 duplicated in the IR regions, of which the gene content and organization are similar to the other Poaceae cp genomes. We selected 40 potential regions in cp genomes of two Cenchrus species and one Korean Pennisetum species to develop new single nucleotide polymorphism (SNP) markers for identifying C. longispinus based on amplification-refractory mutation system (ARMS) technique. The markers, inferred from SNP in matK and ndhF genes, show effectiveness to recognize C. longispinus from C. echinatus and Korean native species Pennisetum alopecuroides (L.) Spreng.

• Journal of Plant Biotechnology
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• v.44 no.2
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• pp.135-141
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• 2017

Cudrania tricuspidata Bureau is a widely used medicinal perennial woody plant. For conservation and germplasm utilization of the plant, it is imperative to obtaining information regarding the genetic diversity of the plant populations. Although C. tricuspidata is an important medicinal plant registered in South Korea, no molecular markers are currently available to distinguish Korean-specific ecotypes from other ecotypes of different countries. In this study, we developed single nucleotide polymorphism (SNP) markers derived from chloroplast genomic sequences to identify distinct Korean-specific ecotypes of C. tricuspidata via the amplification refractory mutation system (ARMS)-PCR analyses. Molecular authentication of twelve C. tricuspidata ecotypes from different regions was performed, using DNA sequences in the trnL-F chloroplast intergenic region. The SNP markers developed in this study are useful for rapidly identifying specific C. tricuspidata ecotypes from different regions.

• Journal of Plant Biotechnology
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• v.48 no.1
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• pp.26-33
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• 2021

Angelica is a widely used medicinal and perennial plant. Information on the genetic diversity of Angelica populations is essential for their conservation and germ plasmic utilization. Although Angelica is an important medicinal plant species registered in South Korea, no molecular markers are currently available to distinguish it from other similar species from different countries. This developed single nucleotide polymorphism (SNP) markers derived from nuclear ribosomal DNA internal transcribed spacer regions genomic sequences to identify distinct Korean-specific Angelica species via amplification refractory mutation system (ARMS)-PCR curve analyses. We performed molecular authentication of different kinds of Korean-specific Angelica species such as A. gigas Nakai and A. gigas Jiri using DNA sequences in the ITS intergenic region. The SNP markers developed in this study are useful for rapidly identifying specific Angelica species from different countr.

• Proceedings of the KIEE Conference
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• 2004.11c
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• pp.644-646
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• 2004

The closed loop identification(CLID) is a very useful method for on-line applications since it can identify the plant in the closed-loop system composed of the plant and the controller. There are some literatures on CLID, but they and mainly focused on SISO(Single-Input/Single-Output) problem. In this paper, a CLID method is proposed for MIMO(Multi-Input/Multi-Output) systems. The CLID method is applied to a MIMO benchmark plant, TRMS(Twin-Rotor MIMO System). To illustrate the performance of the closed-loop system identification., unit step responses in the TRMS are represented and compared with the open-loop identification via some simulation.