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Rapid Propagation through Tissue Culture of Cudrania tricuspidata, Medicinal Plant

  • Lee, Cheul-Ho;Min, Ji-Yun;Jung, Ha-Na;Kim, Kyu-Sick;Choi, Myung-Suk
    • Korean Journal of Medicinal Crop Science
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    • v.15 no.5
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    • pp.315-318
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    • 2007
  • An effective rapid propagation method was established through in vitro cultures of the medicinal plant, Cudrania tricuspidata. In vitro plantlets were obtained from in vitro germinated seeds. The various levels of cytokinins (BAP, Kinetin and TDZ) were tested on multiple shoot formation from plantlets. BAP (1.0 mg/l) treatment induced highest number of multiple shoots. Single shoot cultures gave higher initial shoot numbers than 5 shoots per culture. Among the various culture media, the shoot elongation was optimal on 2 MS basal medium without growth regulators. The IAA (2.0 mg/l) treatment induced highest number of roots. IBA (2.0 mg/l) treatment more promoted in vitro root growth than other concentrations. Rooted shoots were transferred directly to small pots with an artificial soil and successfully acclimatized.

First Report of an Unrecorded Nematode-Trapping Fungus Species Monacrosporium phymatopagum in Korea

  • Wu, Hai-Yan;Kim, Dong-Geun
    • The Plant Pathology Journal
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    • v.26 no.3
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    • pp.264-266
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    • 2010
  • A fungus that can capture nematodes by means of sessile adhesive knobs was isolated from rotten wood in Uiseong, Korea. It was found to produce single, spindle-shaped, 3-4 septate (commonly 4-septate) conidia, $44.8\;{\mu}m$ (range, $41.6-50.1\;{\mu}m$) long and $13.3\;{\mu}m$ (range, $10.7-15.4\;{\mu}m$) wide. Conidiophores were found to be hyaline, erect, straight, and $202.7-245.7\;{\mu}m$ high. On the basis of these morphological features, the fungus was identified as Monacrosporium phymatopagum. This is the first report of M. phymatopagum in Korea which can be a potential biological control resource of plant parasitic nematode.

Story of Johnsongrass Mosaic (Poty)virus in Australia

  • Oh, Hae-Young
    • Journal of Life Science
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    • v.12 no.2
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    • pp.61-74
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    • 2002
  • One of the major aims in studying plant viruses is to minimise the development of symptoms in infected plants. With the advent of in vitro transcript mediated research on plant viruses, substantial progress has been made. This article describes the biology of a plant specific RNA virus, Johnsongrass mosaic virus (JGMV), important to Australian sorghum and corn agriculture and, in particular, at a molecular level which of the RNA sequences in its genome that make it possible for the virus to move from cell to cell, and eventually spread systemically throughout the entire plant. The JGMV has caused considerable yield losses in maize and sorghum over a number of years in Australia. Incidents where 100% of the crop has been infected are on record. The use of this virus is convenient under laboratory conditions because it can be readily transmitted by mechanical inoculation with infected leaf sap, which obviates the need for maintaining aphid colonies. The JGMV is a single stranded positive sense RNA virus.

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Model Reference Adaptive Control Using Non-Euclidean Gradient Descent

  • Lee, Sang-Heon;Robert Mahony;Kim, Il-Soo
    • Transactions on Control, Automation and Systems Engineering
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    • v.4 no.4
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    • pp.330-340
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    • 2002
  • In this Paper. a non-linear approach to a design of model reference adaptive control is presented. The approach is demonstrated by a case study of a simple single-pole and no zero, linear, discrete-time plant. The essence of the idea is to generate a full non-linear model of the plant dynamics and the parameter adaptation dynamics as a gradient descent algorithm with respect to a Riemannian metric. It is shown how a Riemannian metric can be chosen so that the modelled plant dynamics do in fact match the true plant dynamics. The performance of the proposed scheme is compared to a traditional model reference adaptive control scheme using the classical sensitivity derivatives (Euclidean gradients) for the descent algorithm.

Cumambrin A in Chrysanthemum boreale Makino Preparation, X-ray Crystal Structure and $^{13}C-$ and $^1H$-NMR Study of Cumambrin A

  • Park, Ki-Hun;Jang, Dae-Sik;Choi, Sang Uk;Nam, Sang-Hae;Shiro, Mooto;Yang, Min-Suk
    • Korean Journal of Pharmacognosy
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    • v.27 no.3
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    • pp.207-211
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    • 1996
  • Cumambrin A has been isolated from the dried flowers of Chrysanthemum boreale Makino. The complete $^1H$ and $^{13}C$ NMR assignment of cumambrin A was achieved from two-dimensional $^1H$-$^1H$ COSY and $^{13}C$-$^1H$ COSY spectra with the aid of homonuclear and heteronuclear double resonance experiments. The its structure has been verified by single crystal X-ray diffraction.

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The Distribution of Plant Communities on Water Table along the Bukhan Riverside in Chun-seong (춘성지구 북한강유역의 지하수위에 따른 식물군락의 운적적 변화)

  • 이성규
    • Journal of Plant Biology
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    • v.16 no.3_4
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    • pp.1-6
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    • 1973
  • The distribution of plant communities along the Bukhan Riverside in Chun-seong was studied by means of the methods fo Curtis and Pattern. The communities were arranged in a single-dimensioned ordination on the basis of the importance values of the persistent species. Leading dominant species, form the 'low' level to the 'high' levels of the ordination gradient, were Phalaris arundinacea, Salix gilgiana, Artemisia feddei, Zoysia japonica, Pennisetum japonicum, and Arundinella hirta. These species from a continuum from the river to the dry grassland depending upon available soil-moisture. Standing crops in terms of dry weight and the nitrogen content of the above-ground parts of these species showed a good relationship with gradients of available soil-moisture.

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A Study on Reasonable Improvement of Legal Requirement for Fire Proofing for Steel Structures in a Chemical Plant (화학플랜트에서 철골구조물 내화기준의 합리적인 개선에 관한 연구)

  • Hwang, Soon-Yong;Char, Soon-Chul;Kang, Kyung-Sik
    • Journal of the Korea Safety Management & Science
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    • v.9 no.1
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    • pp.51-63
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    • 2007
  • It is noted that the disaster such as fire, explosion, collapse has been rapidly increased caused by strength deterioration of steel structures at petrochemical plant during fire. In this regard, it is stressed out that the legal requirement for fire proofing for steel structures at petrochemical plant should be carefully reviewed since the current legal requirement such as Industrial Safety & Health Law, Architectural Law has a conflict and different way of approach. In addition, it is our point of view that the present law should be revised to consolidate into single law including engineering design criteria to reflect unreasonable legal requirement. It is further our point of view that the performance certificate for fire proofing like UL-1709, basis of maintenance should be appropriately and reasonably provided in line with global practice.

Agrobacterium-mediated Transformation of Rice 'Ilmibyeo' using HPT Selection Maker Gene

  • Guo, Jia;Cho, Joon-Hyeong;Jo, Hye-Jeong;Seong, Eun-Soo;Wang, Myeong-Hyeon
    • Korean Journal of Plant Resources
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    • v.20 no.3
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    • pp.242-246
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    • 2007
  • This study was conducted to produce the transgenic plant of rice. We obtained Agrobacterium AGL1 harbaring pCambial 300 vector with HPT gene. We carried out PCR analysis of 22 ea putative transgenic rice to investigate transformed lines. The 3 ea transgenic lines were detected insertion of HPT gene. Transgenic lines selected from PCR analysis were performed by Southern blot. From Southern blot, we obtained that two transgenic lines detected single band. We are going to study the method improving of cotransformation as well as transformation efficiency in rice.

Root Characters and Freguency of Multi-stem Ginseng (다경인삼의 근형질특성과 경수발현빈도)

  • 최광태;안상득;신희석;천성룡
    • Journal of Ginseng Research
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    • v.8 no.2
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    • pp.82-90
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    • 1984
  • The Present study was undertaken to obtain the basic information on the development of multistem varieties of ginseng. The root weight per plant of multi-stem ginseng was hi燥or than that of single stem ginseng, and it was found that the greater variance due to the growing area was clear in the frequency of multistem plant. The broad heritability estimate for the number of steams was lower with the increase of the age of ginseng. The number of stems per plant was positively correlated with the number of branch roots, number of dormancy buds, and root weight. However, the root diameter was negatively correlated with the number of stems is per plant. Based on path analysis, the number of branch roots and dormancy buds showed the maximum indirect positive effects on the number of stems.

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Selection of RAPD Markers for Phytophthora infestans and PCR Detection of Phytophthora infestans from Potatoes

  • Kim, Kyung-Su;Lee, Youn-Su
    • Journal of Microbiology
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    • v.39 no.2
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    • pp.126-132
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    • 2001
  • For rapid and secure differentiation of P. infestans from other Phytophthora species, two fragments obtained from randomly amplified polymorphic DNA (RAPD) profiles were selected as markers. Also, primers for in polymerase chain reaction (PCR) to detect P. infestans specifically were developed by analyzing the sequences of ITSII regions in rDNA of Phytophthora species. The primers, PISP-1 and ITS3 amplified a single. Fragment 450 bp of about in P. infestans, but not in other fungal or bacterial isolates. Annealing temperatures and template DNA quantities were varied for the optimization of PCR conditions. From the result of the PCR detection study, species-specific primers were selected under annealing temperatures ranging from 55$^{\circ}C$ to 61$^{\circ}C$, and template DNA levels ranging from 10 pg to 100 ng.

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