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A Comparison of Sonication and Microwave-assisted Extraction Method for Speciation of Arsenic in Fish Tissue, DORM-2 (어류중 비소의 종분화 분석을 위한 초음파 추출법과 마이크로파 추출법의 비교)

  • Yoon, Cheol-Ho;Park, Yong-Chul;Hong, Jong-Ki
    • Analytical Science and Technology
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    • v.16 no.2
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    • pp.134-142
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    • 2003
  • Comparison of a microwave-assisted extraction with sonication extraction was performed for arsenic speciation in fish tissue with chromatographic separation and inductively coupled plasma mass spectrometry detection. The detection limits of arsenicals with ultrasonic nebulizerand cross-flow nebulizer were shown to be similar. The arsenicals investigated were arsenobetaine (AsB), arsenite [As(III)], dimethylarsine acid (DMA), monomethylarsonic acid (MMA), arsenate [As(v)], and phenylarsonic acid (PAA). Quantitative extraction of arsenicals from dogfish muscle, DORM-2, standard reference material of NRCC (National Research Council of Canada) was achieved using 50% (v/v) methanol-water in both extraction methods. Extraction efficiency of arsenobetaine in both methods is greater than 82% with RSDs on replicates of less than 5%. The concentrations of AsB determined in extract of microwave assisted extraction and sonication methods were $14.18{\pm}0.42mg\;kg^{-1}$ and $13.54 {\pm}0.84mg\;kg^{-1}$, respectively. And the concentrations of DMA were $0.45{\pm}0.06mg\;kg^{-1}$ and $0.44{\pm}0.06mg\;kg^{-1}$, respectively.

Impact Characteristics of Multi-Density Insoles for DM Shoes (당뇨화 다밀도 안창의 충격특성)

  • 금영광;정임숙;강성재;김영길;김명웅;김영호
    • Journal of Biomedical Engineering Research
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    • v.24 no.1
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    • pp.31-36
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    • 2003
  • Impact characteristics of six DM(Diabetes Mellitus) shoe insole materials (Podian, Plastazote black, Plastazote white, Flexible PU foam, Podialene 200 blue and Podia flex) and three multi-density insoles (AP, OS and PW insoles) were determined in the present study, using a self-designed impact measurement system. The coefficient of restitution, the median frequency and the attenuation index were calculated for each material, based on impact forces and linear accelerations. Podian revealed the superiority in the coefficient of restitution and the attenuation index. The median frequency of the Flexible PU foam was the smallest. Results also showed that the heel region was the most impact-attenuated among other areas in the insole. OS insole showed the better characteristics in the coefficient of restitution and the median frequency. but there was no significant difference in the attenuation index. Similar impact characteristics were found in all areas in PW insole. since it was basically of the same dual-density polyurethane.

Quality Characteristics of Muffin Added Red Yeast Rice Flour (홍국분말을 첨가한 머핀의 품질 특성)

  • Park, Sung-Hee;Lim, Seong-Il
    • Korean Journal of Food Science and Technology
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    • v.39 no.3
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    • pp.272-275
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    • 2007
  • Red yeast rice flour, which is a natural functional material, was used in muffin production at different concentrations ranging from 0 to 10%. The initial pasting temperature was not affected by the addition of red yeast rice flour, whereas peak viscosity and final viscosity decreased with increasing concentrations. The lightness value of the muffins decreased, however, the redness value increased with increasing amounts of red yeast rice flour. The bulk of the muffins was generally reduced by the addition of red yeast rice flour. At the 10% concentration, hardness and viscosity were similar to the control, and there were no changes in springiness or cohesiveness. For the sensory evaluation, parameters such as appearance, color, texture, taste, and overall acceptability increased significantly from the control at the 3% concentration of red yeast rice flour, but flavor decreased with increasing amounts of red yeast rice flour. Based on these results and the sensory evaluation specifically, the optimal amount of red yeast rice flour added to the muffins was the 3% concentration.

Stress Analysis of a Trunnion Ball Valve for Ball Weight Reduction (이축 볼밸브의 볼 경량화를 위한 응력해석)

  • Kim, Hyung-Woo;Cho, Su-gil;Park, Jane;Lee, Jaehwan
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.2
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    • pp.373-380
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    • 2020
  • A valve product can be over-designed or too heavy. Finite element analysis was performed using ANSYS for two and three-dimensional ball valve models, and the ball weight was reduced by optimization within the allowable design criteria. The ball is structurally safe according to the computed stress values, which are within the material's admissible stress. The weight was reduced by about 22%, and the structural safety factor was 1.25. The structural safety of the seat insert and ring, which are used to prevent leakage, was confirmed through finite element analysis. It is shown that the two-dimensional analysis can result in similar values to the three-dimensional analysis for the axisymmetric structure. The redesign of the valve is not included in the results since such changes require a whole new design process, including all valve components.

Understanding the Flow Properties by a Numerical Modeling in the South Sea of Korea (수치모델을 이용한 한국 남해의 유동특성 이해)

  • Bae, Sang-Wan;Kim, Dong-Sun
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.18 no.4
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    • pp.295-307
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    • 2012
  • In order to understand the flow properties of the South Sea of Korea, tidal currents, wind-driven currents, density-driven currents and residual flows were investigated by using 3-dimensional numerical model(POM). In offshore regions, tide-induced residual current tends to flow eastward during the spring tide and westward during the neap tide. Total residual flow is irregular due to the bottom topography in the coastal area. The density-driven currents in the coastal area showed to be relatively weak, with little seasonal differences. The special tendency was apparent in the open sea. That is, the flow in the offshore regions showed results similar to that of the Tsushima current. The wind-driven currents in the coastal area showed to be much stronger than in offshore regions. Vertically, the flow of the surface layer was much stronger than that of the bottom layer. Through these results, material transport and diffusion in the south coast, as a basis for predicting the spread of use is expected to be available.

Differentiated Human Embryonic Stem Cells Enhance the In vitro and In vivo Developmental Potential of Mouse Preimplantation Embryos

  • Kim, Eun-Young;Lee, Keum-Sil;Park, Se-Pill
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.9
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    • pp.1152-1158
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    • 2010
  • In differentiating human embryonic stem (d-hES) cells there are a number of types of cells which may secrete various nutrients and helpful materials for pre-implantation embryonic development. This study examined whether the d-hES could function as a feeder cell in vitro to support mouse embryonic development. By RT-PCR analysis, the d-hES cells revealed high expression of three germ-layered differentiation markers while having markedly reduced expression of stem cell markers. Also, in d-hES cells, LIF expression in embryo implantation-related material was confirmed at a similar level to undifferentiated ES cells. When mouse 2PN embryos were cultured in control M16 medium, co-culture control CR1aa medium or co-cultured with d-hES cells, their blastocyst development rate at embryonic day 4 (83.9%) were significantly better in the d-hES cell group than in the CR1aa group (66.0%), while not better than in the M16 group (90.7%)(p<0.05). However, at embryonic days 5 and 6, embryo hatching and hatched-out rates of the dhES cell group (53.6 and 48.2%, respectively) were superior to those of the M16 group (40.7 and 40.7%, respectively). At embryonic day 4, blastocysts of the d-hES cell group were transferred into pseudo-pregnant recipients, and pregnancy rate (75.0%) was very high compared to the other groups (M16, 57.1%; CR1aa, 37.5%). In addition, embryo implantation (55.9%) and live fetus rate (38.2%) of the d-hES cell group were also better than those of the other groups (M16, 36.7 and 18.3%, respectively; CR1aa, 23.2 and 8.7%, respectively). These results demonstrated that d-hES cells can be used as a feeder cell for enhancing in vitro and in vivo developmental potential of mouse pre-implantation embryos.

Simultaneous Determination of Polycyclic Aromatic Hydrocarbons and Their Nitro-derivatives in Airborne Particulates by Using Two-dimensional High-performance Liquid Chromatography with On-line Reduction and Fluorescence Detection

  • Boongla, Yaowatat;Orakij, Walaiporn;Nagaoka, Yuuki;Tang, Ning;Hayakawa, Kazuichi;Toriba, Akira
    • Asian Journal of Atmospheric Environment
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    • v.11 no.4
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    • pp.283-299
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    • 2017
  • An analytical method using high-performance liquid chromatography (HPLC) with fluorescence (FL) detection was developed for simultaneously analyzing 10 polycyclic aromatic hydrocarbons (PAHs) and 18 nitro-derivatives of PAHs (NPAHs). The two-dimensional HPLC system consists of an on-line clean-up and reduction for NPAHs in the 1st dimension, and separation of the PAHs and the reduced NPAHs and their FL detection in the 2nd dimension after column-switching. To identify an ideal clean-up column for removing sample matrix that may interfere with detection of the analytes, the characteristics of 8 reversed-phase columns were evaluated. The nitrophenylethyl (NPE)-bonded silica column was selected because of its shorter elution band and larger retention factors of the analytes due to strong dipole-dipole interactions. The amino-substituted PAHs (reduced NPAHs), PAHs and deuterated internal standards were separated on polymeric octadecyl-bonded silica (ODS) columns and by dual-channel detection within 120 min including clean-up and reduction steps. The limits of detection were 0.1-9.2 pg per injection for PAHs and 0.1-140 pg per injection for NPAHs. For validation, the method was applied to analyze crude extracts of fine particulate matter ($PM_{2.5}$) samples and achieved good analytical precision and accuracy. Moreover, the standard reference material (SRM1649b, urban dust) was analyzed by this method and the observed concentrations of PAHs and NPAHs were similar to those in previous reports. Thus, the method developed here-in has the potential to become a standard HPLC-based method, especially for NPAHs.

A Study on the Mechanical Properties of Al2O3 Cutting Tools by DLP-based 3D Printing (DLP 기반 3D 프린팅으로 제조된 Al2O3 절삭공구의 기계적 물성 연구)

  • Lee, Hyun-Been;Lee, Hye-Ji;Kim, Kyung-Ho;Kim, Kyung-Min;Ryu, Sung-Soo;Han, Yoonsoo
    • Journal of Powder Materials
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    • v.26 no.6
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    • pp.508-514
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    • 2019
  • In the development of advanced ceramic tools, material improvements and design freedom are critical in improving tool performance. However, in the die press molding method, many factors limit tool design and make it difficult to develop innovative advanced tools. Ceramic 3D printing facilitates the production of prototype samples for advanced tool development and the creation of complex tooling products. Furthermore, it is possible to respond to mass production requirements by reflecting the needs of the tool industry, which can be characterized by small quantities of various products. However, many problems remain in ensuring the reliability of ceramic tools for industrial use. In this study, alumina inserts, a representative ceramic tool, was manufactured using the digital light process (DLP), a 3D printing method. Alumina inserts prepared by 3D printing are pressurelessly sintered under the same conditions as coupon-type specimens prepared by press molding. After sintering, a hot isostatic pressing (HIP) treatment is performed to investigate the effects of relative density and microstructure changes on hardness and fracture toughness. Alumina inserts prepared by 3D printing show lower relative densities than coupon specimens prepared by powder molding but indicate similar hardness and higher fracture toughness values.

Use of CGF and CCW as Medium Components for Commercial Production of Plug Seedlings of Seogun Tomato (토마토 공정묘의 상업적 생산을 위한 배지구성물질로서 발포유리와 탄화 밤나무 칩의 이용)

  • 황승재;정병룡
    • Journal of Bio-Environment Control
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    • v.13 no.2
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    • pp.81-89
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    • 2004
  • This study was conducted in a commercial plug glasshouse in Sacheon to examine the possibility of producing tomato plug seedlings using various growing media containing cellular glass foam (CGF)and carbonized chestnut woodchips (CCW) as medium components. Plug seedlings of 'Seogun' tomato were grown in media containing 50% CCW+50% peatmoss, 33% CGF+67% peatmoss, and 50% peatmoss+50% granular rockwool. A commercial plug medium (Tosilee) was used as the control. All seeds were sown in 200 cell plug trays on November 28, 2001. Seedling growth was measured at 31 days after sowing. Each treatment showed a similar growth result as compared to the control. Plant height, root grade, fresh weight, and air space and bulk density of the medium were significantly greater in the 33% CGF+67% peatmoss treatment than those in the other media. However, growth was slightly suppressed in the 50% CCW+50% peatmoss. pH and EC of the media were the highest in the control treatment, although no toxicity symptoms had been observed. The results suggest that perlite can be replaced with a new material such as CGF in the commercial scale production of plug seedlings of 'Seogun' tomato.

Soil physio-chemical properties of Mt. NamSan on Kyungju in Korea (경주 남산의 토양 이화학적 특성)

  • Hur, Tae-Chul;Joo, Sung-Hyun
    • Current Research on Agriculture and Life Sciences
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    • v.23
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    • pp.53-60
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    • 2005
  • This study was carried out in order to produce useful material for the forest multiple use and forest protection management by soil physio-chemical analysis of studied area in Mt. Namsan. The result of soil physio-chemical analysis and statical analysis represented as following. In side of physical properties of forest soil in Mt. Namsan, Soil depth was average 31.4cm and available soil depth was average 20.0cm. Soil type was Brown forest soil that representative soil type in Korea. Soil texture was sandy loom(SL) except valley area. In side of chemical properties, the range of soil acidity was 4.29 ~ 5.19 (average 4.76), organic matter content was 3.17% that compared the lowest value to organic matter content of Korea forest soil. Available phosphorus was 3.64ppm that was lower than others forest soil. Exchangeable cation content was similar to the Korea brown forest soil. Cation exchange capacity(CEC) was $8.22cmol_c/kg$ in Mt. Narnsan.

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