• Journal of Species Research
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• 제12권3호
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• pp.203-211
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• 2023

Five ciliate species of Euplotes were isolated from fresh and coastal water during a sampling survey to identify unrecorded ciliates in South Korea. Their morphology was investigated using live observation, protargol and "wet" silver nitrate staining methods. Brief descriptions and microphotographs of each species and a comparison with related species are provided. Euplotes focardii is characterized by an average size of 65×47 ㎛ after protargol impregnation, 6 dorsal and 3 ventral ridges and dorsal argyrome pattern of double-eurystomus type. Euplotes nobilii shows an average size of 34×20 ㎛ after protargol staining, 6 dorsal and 3 ventral ridges and dorsal argyrome pattern of double-patella type. Euplotes octocarinatus, the only freshwater species described in the present study, is characterized by an average size of 66×46 ㎛ after protargol impregnation, 6 dorsal and 3 ventral ridges and dorsal argyrome pattern of double-patella type. Euplotes petzi has an average size of 43×30 ㎛ after protargol staining, a macronucleus hook-shaped and dorsal argyrome pattern in double-patella type. Euplotes raikovi is characterized by an average size of 40×24 ㎛ after protargol staining, 6 dorsal and 3 ventral ridges and dorsal argyrome pattern of double-patella type.

• Journal of Species Research
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• 제10권3호
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• pp.301-320
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• 2021

During a field survey of indigenous Korean ciliates, we collected 21 unrecorded species from aquatic (freshwater and brackish water [salinity about 1.0‰] samples) and terrestrial(moss and soil samples) habitats. The morphology of these species was studied based on live observation, protargol, silver carbonate and silver nitrate impregnation, and scanning electron microscope. These species belong to seven classes as follows: 1) class Heterotrichea - Blepharisma seculum; 2) class Spirotrichea - Birojimia terricola, Bistichella variabilis, Deviata bacilliformis, Oxytricha paragranulifera, Parabistichella cf. variabilis, Steinia sphagnicola, Stichotricha aculeata, Stylonychia cf. notophora, and Tetmemena bifaria minima; 3) class Armophorea - Metopus es; 4) class Litostomatea - Acropisthium mutabile, Pseudomonilicaryon japonicum, and Rimaleptus alpinus; 5) class Phyllopharyngea - Podophrya bivacuolata; 6) class Nassophorea - Drepanomonas pauciciliata; and 7) class Oligohymenophorea - Dexiostoma campylum, Frontonia atra, Histiobalantium natans viridis, Opisthonecta minima, and Tetrahymena rostrata. Here, we provide a brief diagnosis and remark for each species.

• Journal of Species Research
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• 제11권4호
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• pp.278-286
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• 2022

During a field survey of Korean marine ciliates, we collected Pleuronema marinum from a brackish water sample. It is characterized by the presence of a contractile vacuole in mid-body, rather than the subterminal/ terminal contractile vacuole as in other congeners. The cells were examined in vivo and based on protargol and 'wet' silver nitrate impregnation. In addition, the nuclear 18S rRNA gene was sequenced using a single cell. The Korean population morphologically and molecularly resembles a Chinese population of P. marinum. Historical review of the species concludes that 1) two or more species have been assigned into P. marinum, 2) the position of contractile vacuole (e.g., in mid-body) is a valid character state, and 3) P. marinum is probably a rare species. Here we provide a monographic treatment of P. marinum to clarify the issue and for further studies of relevant species. Considering there are about 40 nominal species and complex nomenclatural acts in the genus Pleuronema, further studies should provide descriptions based on protargol and 'wet' silver impregnation with marker gene(s).

• Journal of Species Research
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• 제5권3호
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• pp.463-467
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• 2016

We collected two unrecorded Colpoda species - C. cucullus $M\ddot{u}ller$, 1773 and C. inflata (Stokes, 1885) Kahl, 1931 from Jeju-do Province (Sarabong Park, $33^{\circ}31^{\prime}N$ $126^{\circ}32^{\prime}E$) in November, 2014. We described these species based on observations of live and impregnated (protargol and silver nitrate impregnation) specimens.

• Animal Systematics, Evolution and Diversity
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• 제26권1호
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• pp.21-27
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• 2010

Two marine euplotid ciliates, i.e. Euplotes cristatus Kahl, 1932 and E. minuta Yocom, 1930, were collected from the public waterfront of Incheon on the Yellow Sea and from the Songjeong Beach, Busan, in the Strait of Korea, respectively. These two species were verified as unrecorded species in Korea. These species were described based on live observation, protargol impregnation, and silver nitrate impregnation. In addition, the small subunit ribosomal DNA (SSU rDNA) sequences of the two species were compared with previously known sequences of the Euplotes species. Euplotes cristatus has an elongated oval form, size in vivo of $60-84{\times}38-68\;{\mu}m$, 35-50 adoral zone of membranelles (AZM), 10 frontoventral cirri (FVC), 5 transverse cirri (TC), 4-5 caudal cirri (CC), 8 dorsal kineties (DK), 10-16 dorsal cilia of middle DK, and silverline system of single-vannus type. Euplotes minuta has a small ovoid form ($44-53{\times}26-35\;{\mu}m$ in vivo), 31-41 AZM, 10 FVC, 5 TC, 4 CC, 9 DK, 10-12 dorsal cilia of middle DK, and silverline system of single-vannus type.

• 한국대기환경학회지
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• 제9권2호
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• pp.160-167
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• 1993

As the major methanol fueled vehicle exhaust components, formaldehyde & methanol conversion over the existing commercial 3-way catalyst was examined in a labolatory tains different Ag loadings on commercial 3-way catalyst, and german commercial catalysts for methanol engine exhaust manufactured by a commercial manufacturer. Silver catalysts were prepared by the wet impregnation of silver nitrate solution on commercial 3-way catalyst. These catalysts were characterized with BET Surface area and pore size distribution. In general, the formaldehyde(HCHO) conversion of the tested catalysts was similar to that of methanol$(CH_3OH)$. At 100$^\circ$C, which is equivalent to the cold start condition, 5wt% Ag cat. showed the most excellent HCHO and $CH_3OH$ conversion. The order of activity for conversion of HCHO & $CH_3OH$ to carbon dioxide and water vapor was as follows ; 5wt% Ag/3-way cat.>2wt% Ag/3-way cat.>german cat. front(1) > german cat. rear(2) > 10wt% Ag/3-way cat.> commercial 3-wat catalyst. However there was no significant activity difference between those tested catalysts in the hot run condition of 400$^\circ$C. Therefore, it could be concluded that the Ag-modified 3-way catalyst was the most effective and practical catalyst system which could be capable of removal the HCHO and methanol at the special condition of low temperature such as cold start condition.

• 한국작물학회지
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• 제50권spc1호
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• pp.228-230
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• 2005

형광 염료와 레이저 겔스캐너 장비를 이용하여 변성아크릴 아마이드 겔에서 전기 영동된 DNA를 신속하고 간편한 방법으로 기존의 은염색법과 비슷한 감도로 검출하고자 하였다. 변성아크릴아마이드 겔을 형광 염료인 SYBR Green (Molecular Probes)이나 Vistra Green (Amersham Bioscience) 0.01 X 희석액 (pH 8)으로 염색한 후 480nm 레이져, 520nm filer 옵션으로 스캔하여 DNA를 검출하였으며, 검출감도는 기존의 은염색법과 비슷하면서 염색 단계를 한 단계로 줄일 수 있었다.

• Animal Systematics, Evolution and Diversity
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• 제27권1호
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• pp.25-33
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• 2011

Three morphospecies of the genus Uronychia, i.e. U. setigera Calkins, 1902, U. binucleata Young, 1922, and U. multicirrus Song, 1997, were collected from the coastal waters of Gumjin-ri on the East Sea and the public waterfront of Incheon on the Yellow Sea in Korea, respectively. These species are described based on live observation, protargol impregnation, silver nitrate impregnation, and their morphometrics. Diagnostic keys for these species are also provided. In addition, their small subunit ribosomal DNA sequences were compared with previously known sequences of Uronychia species. Diagnostics of three Uronychia species are as follows: U. setigera: $50-80\;{\mu}m$ long in vivo, oval-shaped, 2 macronuclear nodules (Ma), 1 spur on the left margin, 11 adoral membranelles (AM) 1, 4 AM2, 1 buccal cirrus (BC), 4 frontal cirri (FC), 3 left marginal cirri (LMC), 2 ventral cirri (VC), 5 transverse cirri (TC), 3 caudal cirri (CC), 6 dorsal kineties (DK), and approximately 23 cilia in the leftmost kinety. U. binucleata: $70-110\;{\mu}m$ long in vivo, oval to slightly rectangular shaped, 2 Ma, 1 micronucleus (Mi), 2 spurs on the posterior region, 11 AM1, 4 AM2, 1 BC, 4 FC, 3 LMC, 2 VC, 5 TC, 3 CC, 6 DK, and approximately 37 cilia in the leftmost kinety. U. multicirrus: $140-200\;{\mu}m$ long in vivo, oval to slightly rectangular shaped, ca. 7 Ma, 1 Mi, 11 AM1, 4 AM2, 1 BC, 4 FC, 3 LMC, approximately 8 VC, 5 TC, 3 CC, and 6 DK. This study presents the first record of this genus in Korea.

• 환경생물
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• 제33권4호
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• pp.375-382
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• 2015

We discovered three soil ciliates of the class Colpodea-Colpoda henneguyi Fabre-Domergue, 1889; C. lucida Greeff, 1888; and Bursaria truncatella $M{\ddot{u}}ller$, 1773 from Obong-ri, Ayajin-ri and Elwang-ri (Korea), respectively. Colpoda henneguyi had the following features: often wider preorally than postorally, size in vivo $60-80{\mu}m{\times}50-70{\mu}m$; extrusomes indistinct in vivo, cylindroid approximately $1{\mu}m$ long; notches caused by deep diagonal groove; yellowish globules on the cortex of the cell; 10-12 postoral kineties; silverline system aspera-type. Colpoda lucida exhibited the following features: broadly reniform, size in vivo $70-90{\mu}m{\times}50-70{\mu}m$; conspicuous extrusomes, $3.5-5{\mu}m$ long in vivo, cylindroid to fusiform; 13-16 postoral kineties; silverline system cucullus-type. Bursaria truncatella had the following features: bursiform, size in vivo $300-470{\mu}m{\times}120-260{\mu}m$; macronucleus coiled with highly variable shapes, $600-1100{\mu}m{\times}30-40{\mu}m$ long in vivo; micronuclei 16-25 in number, approximately $4{\mu}m$ in diameter; extrusomes cylindroid, $3-4{\mu}m$ long in vivo. This is the first report of colpodean ciliates from Korea, and we describe these species based on observations of live and impregnated (protargol and silver nitrate impregnation) specimens.