• Journal of Microbiology and Biotechnology
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• 제11권5호
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• pp.804-811
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• 2001

In many Gram-negative bacteria, autoinducers, such as N-acyl-L-homoserine lactone(acyl-HSL) and its derivative molecules, mediate the cell-density-dependnet expression of certain operons. The current study identified the autoinducers produced by Burkholderia cepacia G4, a trichloroethylene-degrading lagoon isolate, using TLC bioassays with Agrobacterium tumefaciens NT1(pDCI141E33) and Chromobacterium violaceum CVO26, and a GC-MS analysis. The ${R_f}\;and\;{R_t}$ values and mass spectra were compared with those of synthetic compounds. Based on the analyses, it was confirmed that G4 produces N-hexanoyl (C6)-, N-octanoyl (C8)-, N-decanoyl (C10)-, N-dodecanoyl (C12)-HSL, and an unknown active species. The integration of the GC peak areas exhibited a ratio of C8-HSL:C10-HSL:C12-HSL at 3:17:1 with C6-HSL and C10-HSL production at trace and micromolar levels, respectively, in the culture supernatants. Nutants partially defective in producing acyl-HSLs were also partially defective in the biosynthesis of an antibiotic substance. These results indicate that the autoinducer-dependent gene regulation in G4 is dissimilar to the clinical B. cepacia strains isolated from patients with cystic fibrosis.

• 한국발생생물학회지:발생과생식
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• 제16권1호
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• pp.69-75
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• 2012

Hatching occurred in the time dependent manners and strictly controlled. Although, the hatching processes are under the control of muti-embryotrophic factors and the expressed G proteins of cell generate integrated activation, the knowledge which GPCRs are expressed during hatching stage embryos are very limited. In the present study, which G proteins are involved was examined during blastocyst development to the hatching stage. The early-, expanded-, and lobe-stage blastocysts were treated with various $G_{\alpha}$ activators and H series inhibitors, and examined developmental patterns. Pertusis toxin (PTX) improved the hatching rate of the early-stage blastocyst and lobe-formed embryos. Cholera toxin (CTX) suppressed the hatching of the early-stage blastocyst and expanded embryos. The effects of toxins on hatching and embryo development were changed by the H7 and H8. These results mean that PTX mediated GPCRs activation is signaling generator in the nick or pore formation in the ZP. In addition, PTX mediated GPCR activation induces the locomotion of trophectoderm for the escaping. CTX mediate GPCRs activation is the cause of suppression of hatching processes. Based on these data, it is suggested that various GPCRs are expressed in the periimplantation stage embryos and the integration of the multiple signals decoding of various signals in a spatial and temporal manner regulate the hatching process.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제11권8호
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• pp.3904-3917
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• 2017

IP multimedia subsystem (IMS) is an open standardized architecture for delivering multimedia service over IP network in a route-agnostic manner. With the increasing popularity of conversational class service, the delivery of a traffic flow with a certain bandwidth demand over a single network path is either not possible or not cost-effective. Multipath transmission is considered to be a promising solution to provide high-quality delivery service. This paper proposes a software defined service overlay network (SDSON) based multipath transmission framework for IMS, which is complementary to existing network architecture. The framework transforms original two-party session negotiation into three-party session negotiation that supports participants to negotiate multipath transmission capacity and path information by signaling message. Based on existing IETF standards, SIP and SDP are scalable to support these functions. Finally, the proposed framework is fully implemented on open source platform and examined by experiments. Experimental results show that multipath-enabled IMS is an effective way to improve the delivery performance of conversational class service.

• Journal of Plant Biotechnology
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• 제48권3호
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• pp.186-192
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• 2021

The roots of Korean ginseng (Panax ginseng) have a long history of usage as a medicinal drug. Ginsenosides, a group of triterpenioid saponins in ginseng, have been reported to show important pharmacological effects. Many studies have attempted to identify the ginsenoside synthesis pathways of P. ginseng and to increase crop productivity. Recent studies have shown that exogenous gibberellin (GA) treatments promote storage root secondary growth by integration of the modulating cambium stem cell homeostasis with a secondary cell wall-related gene network. However, the dynamic regulation of ginsenoside synthesis-related genes and their contents by external signaling cues has been rarely evaluated. In this study, we confirmed that GA treatment not only enhanced the secondary growth of P. ginseng storage roots, but also significantly enriched the terpenoid biosynthesis process in RNA-seq analysis. Consistently, we also found that the expression of most genes involved in the ginsenoside synthesis pathways, including those encoding methylerythritol-4-phosphate (MEP) and mevalonate (MVA), and the saponin content in both leaves and roots was increased by exogenous GA application. These results can be used in future development of biotechnology for ginseng breeding and enhancement of saponin content.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제15권4호
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• pp.284-288
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• 2009

단백질 상호작용의 예측 및 실험 결과가 대용량으로 배포되면서 바이오 정보 기술 연구자들은 생명체 내의 단백질 상호작용 네트워크를 구성하기 위해 노력하여 왔다. 일반적으로 대용량의 상호작용 데이터들은 많은 오류를 포함한다고 알려져 있으나, 최근 단백질의 물리 화학적 특성 및 구조를 기반으로 한 방법들이 실제 실험과 병행되어 고화질(High resolution)의 결과를 제공하게 되면서, 특정 종에 대한 단백질 상호작용 네트워크가 점차 완성되고 있다. 그러나, 단순 물리적 링크 수준의 단백질 상호작용 네트워크만으로는 특정 병원체의 발병 메커니즘 규명 등과 같은 응용분야의 활용에 한계가 있다. 본 논문에서는 실험을 통하여 보고된 신호 전달 경로(signaling transduction pathway)를 이용하여 단백질 기능 간의 관계를 방향성이 있는 그래프로 표현한 단백질 기능 흐름 모델을 제시한다. 제안하는 모델은 Gene Ontology에서 정의된 molecular function을 정점(vertex)으로 가지고 이들 사이의 관계를 간선(edge)으로 표현함으로써 특정 기능의 전이를 살펴볼 수 있다. 이러한 기능 흐름 모델은 수 만개의 정점(vertex)으로 구성된 단백질 상호작용 네트워크에서 의미 있는 경로를 추출하는 데에 제약 혹은 참조 조건으로 사용될 수 있어 향후 활용도가 클 것으로 기대한다. 평가는 KEGG에서 제공되는 11개의 인간 신호 전달 경로 각각에 대하여 대상 경로를 제외한 나머지로부터 생성된 모델과의 크론바하 알파 계수(Cronbach's alpha)를 측정하였고(${\alpha}=0.67$), 총 1023개의 흐름 중 ${\alpha}=0.6$ 이상의 신뢰도에 대하여 총 765개의 흐름을 가지는 기능 흐름 모델을 최종 구성하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권9호
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• pp.1458-1468
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• 2019

Objective: As one of the most important metabolic organs, the liver plays vital roles in modulating the lipid metabolism. This study was to compare miRNA expression profiles of the Large White liver between two different developmental periods and to identify candidate miRNAs for lipid metabolism. Methods: Eight liver samples were collected from White Large of 70-day fetus (P70) and of 70-day piglets (D70) (with 4 biological repeats at each development period) to construct sRNA libraries. Then the eight prepared sRNA libraries were sequenced using Illumina next-generation sequencing technology on HiSeq 2500 platform. Results: As a result, we obtained 346 known and 187 novel miRNAs. Compared with the D70, 55 down- and 61 up-regulated miRNAs were shown to be significantly differentially expressed (DE). Gene ontology and Kyoto encyclopedia of genes and genomes enrichment analysis indicated that these DE miRNAs were mainly involved in growth, development and diverse metabolic processes. They were predicted to regulate lipid metabolism through adipocytokine signaling pathway, mitogen-activated protein kinase, AMP-activated protein kinase, cyclic adenosine monophosphate, phosphatidylinositol 3 kinase/protein kinase B, and Notch signaling pathway. The four most abundantly expressed miRNAs were miR-122, miR-26a and miR-30a-5p (miR-122 only in P70), which play important roles in lipid metabolism. Integration analysis (details of mRNAs sequencing data were shown in another unpublished paper) revealed that many target genes of the DE miRNAs (miR-181b, miR-145-5p, miR-199a-5p, and miR-98) might be critical regulators in lipid metabolic process, including acyl-CoA synthetase long chain family member 4, ATP-binding casette A4, and stearyl-CoA desaturase. Thus, these miRNAs were the promising candidates for lipid metabolism. Conclusion: Our study provides the main differences in the Large White at miRNA level between two different developmental stages. It supplies a valuable database for the further function and mechanism elucidation of miRNAs in porcine liver development and lipid metabolism.

• Asian-Australasian Journal of Animal Sciences
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• 제21권4호
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• pp.499-509
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• 2008

Foxo1 plays an important role in the integration of hormone-activated signaling pathways with the complex transcriptional cascade that promotes preadipocyte differentiation of clonal cell lines from rodents. We isolated the full-length cDNA of porcine FoxO1 gene using RACE, confirmed by visual Northern blotting. The deduced amino acids indicated 94% and 90% identities with the corresponding human and mice aa. Analysis of the aa sequence, showed that it included a Forkhead domain (aa 167-247), a transmembrane structure domain (aa 90-113), a LXXLL motif (aa 469-473), and 51 Ser, 8 Thr, and 4 Tyr phosphorylation sites, indicating a potential important role for FoxO1 transcriptional activity in vivo. Using the IMpRH panel, we mapped FoxO1 gene to chromosome 11p13. Our data provide basic molecular information useful for the further investigation on the function of FoxO1 gene. Time-course analysis of FoxO1 expressions indicated that levels of mRNA and protein gradually increased from day 0 to 3, and it reached almost maximal level at day 3, then decreased from day 5 to 7 in porcine primary preadipocyte differentiation. After induction by IGF-1, GPDH activity and accumulation of lipid increased, however, expressions of FoxO1 mRNA and protein were inhibited in a dose dependent manner. These results suggest that FoxO1 takes part in porcine preadipocyte differentiation and expressions of FoxO1 were regulated by IGF-1.

• 대한전자공학회논문지TC
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• 제40권10호
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• pp.119-128
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• 2003

인터넷 사용자의 급증, 서비스의 다양화, 전송속도의 고속화, 이용 분야의 확대 등으로 인해 인터넷 트래픽이 매년 급속하게 증가하고 있다. 이러한 수요를 충족시키기 위하여 대용량의 대역폭을 제공하는 WDM 기술과 IP 망을 효율적으로 통합한 MPLS를 기반으로 하는 MPλS 프로토콜이 제안되었다. 본 논문에서는 ns-2 네트워크 시뮬레이터의 기능을 확장하여 MPλS 네트워크 시뮬레이터를 설계하고 구현하였다. 구현한 시뮬레이터는 OXC(Optical Crossconnect) 모듈, 효율적인 파장 할당을 위한 RWA(Routing and Wavelength Assignment) 모듈, 다중 파장 링크 모듈, MPλS 시그널링 및 제어 모듈 등으로 구성된다. 구현한 시뮬레이터 상에서 처리율 및 블로킹 확률 등의 파라미터를 이용하여 MPλS의 성능을 검증하였으며 구현한 시뮬레이터는 시스템 개발 이전 단계에서 제안된 프로토콜의 기능을 검증하는데 유용하게 사용 될 수 있을 것으로 사료된다.

• The Plant Pathology Journal
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• 제29권3호
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• pp.249-259
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• 2013

Burkholderia glumae causes rice grain rot and sheath rot by producing toxoflavin, the expression of which is regulated by quorum sensing (QS). The QS systems of B. glumae rely on N-octanoyl homoserine lactone, synthesized by TofI and its cognate receptor TofR, to activate the genes for toxoflavin biosynthesis and an IclR-type transcriptional regulator gene, qsmR. To understand genome-wide transcriptional profiling of QS signaling, we employed RNAseq of the wild-type B. glumae BGR1 with QS-defective mutant, BGS2 (BGR1 tofI::${\Omega}$) and QS-dependent transcriptional regulator mutant, BGS9 (BGR1 qsmR::${\Omega}$). A comparison of gene expression profiling among the wild-type BGR1 and the two mutants before and after QS onset as well as gene ontology (GO) enrichment analysis from differential expressed genes (DEGs) revealed that genes involved in motility were highly enriched in TofI-dependent DEGs, whereas genes for transport and DNA polymerase were highly enriched in QsmR-dependent DEGs. Further, a combination of pathways with these DEGs and phenotype analysis of mutants pointed to a couple of metabolic processes, which are dependent on QS in B. glumae, that were directly or indirectly related with bacterial motility. The consistency of observed bacterial phenotypes with GOs or metabolic pathways in QS-regulated genes implied that integration RNAseq with GO enrichment or pathways would be useful to study bacterial physiology and phenotypes.

• 한국통신학회논문지
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• 제27권12C호
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• pp.1245-1250
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• 2002

최근 몇 년간 인터넷 사용 환경이 주로 유선 접속에서 점차 무선으로 접속하는 경우가 증가하고 있으며 또한 실시간 응용 데이터의 사용이 늘어나고 있다. 이에 따라 사용자의 이동성을 제공하고 동시에 QoS를 제공하기 위한 무선 네트워크의 연구 및 개발이 필수적이다. IETF는 이미 이러한 이동성 지원을 위한 차세대 프로토콜인 Mobile IPv6와 QoS를 위한 RSVP를 각각 개발하였으며 이것은 앞으로 유선 네트워크뿐만 아니라 무선 네트워크에도 적용되리라 예상된다. 그러나 이 두 가지 메커니즘의 통합과 효율적 연동은 아직까지 제시되지 않았다. 본 논문에서는 우선 빠른 이동성을 지원할 수 있는 무선 네트워크 모델로 최근 IETF에서 제안하고 있는 Mobile IPv6 기반의 셀룰러 IP를 사용하였으며 셀룰러 IP와 기존 RSVP의 적용에 있어서의 문제점으로 이동 호스트의 잦은 핸드오프로 발생하는 RSVP 시그널링 오버헤드 증가 문제와 네트워크 내에서 동일한 응용 데이터에 대한 자원 예약에 있어서의 문제점을 해결하면서 두 가지 프로토콜의 효율적 연동을 위해 IPv6 헤더의 플로우 레이블 필드(flow label field)를 이용하는 개선된 RSVP 메커니즘을 제안하였다.