Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Gibberellins enhance plant growth and ginsenoside content in Panax ginseng

  • Received : 2021.09.04
  • Accepted : 2021.09.23
  • Published : 2021.09.30
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Abstract

The roots of Korean ginseng (Panax ginseng) have a long history of usage as a medicinal drug. Ginsenosides, a group of triterpenioid saponins in ginseng, have been reported to show important pharmacological effects. Many studies have attempted to identify the ginsenoside synthesis pathways of P. ginseng and to increase crop productivity. Recent studies have shown that exogenous gibberellin (GA) treatments promote storage root secondary growth by integration of the modulating cambium stem cell homeostasis with a secondary cell wall-related gene network. However, the dynamic regulation of ginsenoside synthesis-related genes and their contents by external signaling cues has been rarely evaluated. In this study, we confirmed that GA treatment not only enhanced the secondary growth of P. ginseng storage roots, but also significantly enriched the terpenoid biosynthesis process in RNA-seq analysis. Consistently, we also found that the expression of most genes involved in the ginsenoside synthesis pathways, including those encoding methylerythritol-4-phosphate (MEP) and mevalonate (MVA), and the saponin content in both leaves and roots was increased by exogenous GA application. These results can be used in future development of biotechnology for ginseng breeding and enhancement of saponin content.

Keywords

Acknowledgement

This research was supported by Chungbuk National University Korea National University Development Project (2021).

References

  1. Baeg IH, So SH (2013) The world ginseng market and the ginseng (Korea). J Ginseng Res 37(1):1-7 https://doi.org/10.5142/jgr.2013.37.1
  2. Benveniste P (2004) Biosynthesis and accumulation of sterols. Annu Rev Plant Biol 55:429-457 https://doi.org/10.1146/annurev.arplant.55.031903.141616
  3. Bergman ME, Davis B, Phillips MA (2019) Medically Useful Plant Terpenoids: Biosynthesis, Occurrence, and Mechanism of Action. Molecules 24(21)
  4. Cheng H, Song S, Xiao L, Soo HM, Cheng Z, Xie D, Peng J (2009) Gibberellin acts through jasmonate to control the expression of MYB21, MYB24, and MYB57 to promote stamen filament growth in Arabidopsis. PLoS Genet 5(3):e1000440 https://doi.org/10.1371/journal.pgen.1000440
  5. Heinrich M, Hettenhausen C, Lange T, Wunsche H, Fang J, Baldwin IT, Wu J (2013) High levels of jasmonic acid antagonize the biosynthesis of gibberellins and inhibit the growth of Nicotiana attenuata stems. Plant J 73(4):591-606 https://doi.org/10.1111/tpj.12058
  6. Hong CP, Kim J, Lee J, Yoo S-i, Bae W, Geem KR, Yu J, Jang I-b, Jo IH, Cho H, Shim D, Ryu H (2021) Gibberellin signaling promotes the secondary growth of storaage roots in Panax ginseng. Int J Mol Sci 22:8694 https://doi.org/10.3390/ijms22168694
  7. Hong J, Kim H, Ryu H (2018) Identification of ABSCISIC ACID (ABA) signaling related genes in Panax ginseng. J Plant Biotechnol (45):306-314
  8. Huang C, Qian ZG, Zhong JJ (2013) Enhancement of ginsenoside biosynthesis in cell cultures of Panax ginseng by N,N'-dicyclohexylcarbodiimide elicitation. J Biotechnol 165(1):30-36 https://doi.org/10.1016/j.jbiotec.2013.02.012
  9. Hwang H, Lee HY, Ryu H, Cho H (2020) Functional Characterization of BRASSINAZOLE-RESISTANT 1 in Panax Ginseng (PgBZR1) and Brassinosteroid Response during Storage Root Formation. Int J Mol Sci 21(24)
  10. Jo IH, Lee J, Hong CE, Lee DJ, Bae W, Park SG, Ahn YJ, Kim YC, Kim JU, Lee JW, Hyun DY, Rhee SK, Hong CP, Bang KH, Ryu H (2017) Isoform Sequencing Provides a More Comprehensive View of the Panax ginseng Transcriptome. Genes (Basel) 8(9)
  11. Kaneko H, Nakanishi K (2004) Proof of the mysterious efficacy of ginseng: basic and clinical trials: clinical effects of medical ginseng, korean red ginseng: specifically, its anti-stress action for prevention of disease. J Pharmacol Sci 95(2):158-162 https://doi.org/10.1254/jphs.FMJ04001X5
  12. Kim NH, Jayakodi M, Lee SC, Choi BS, Jang W, Lee J, Kim HH, Waminal NE, Lakshmanan M, van Nguyen B, Lee YS, Park HS, Koo HJ, Park JY, Perumal S, Joh HJ, Lee H, Kim J, Kim IS, Kim K, Koduru L, Kang KB, Sung SH, Yu Y, Park DS, Choi D, Seo E, Kim S, Kim YC, Hyun DY, Park YI, Kim C, Lee TH, Kim HU, Soh MS, Lee Y, In JG, Kim HS, Kim YM, Yang DC, Wing RA, Lee DY, Paterson AH, Yang TJ (2018) Genome and evolution of the shade-requiring medicinal herb Panax ginseng. Plant Biotechnol J 16(11):1904-1917 https://doi.org/10.1111/pbi.12926
  13. Kim J, Shin WR, Kim YH, Shim D, Ryu H (2021) Functional characterization of gibberellin signaling related genes in Panax ginseng. J Plant Biotech 48(3):148-155 https://doi.org/10.5010/JPB.2021.48.3.148
  14. Lee CH, Kim JH (2014) A review on the medicinal potentials of ginseng and ginsenosides on cardiovascular diseases. J Ginseng Res 38(3):161-166 https://doi.org/10.1016/j.jgr.2014.03.001
  15. Lee DK, Park S, Long NP, Min JE, Kim HM, Yang E, Lee SJ, Lim J, Kwon SW (2020) Research Quality-Based Multivariate Modeling for Comparison of the Pharmacological Effects of Black and Red Ginseng. Nutrients 12(9)
  16. Lee J, Shim D, Moon S, Kim H, Bae W, Kim K, Kim YH, Rhee SK, Hong CP, Hong SY, Lee YJ, Sung J, Ryu H (2018) Genome-wide transcriptomic analysis of BR-deficient Micro-Tom reveals correlations between drought stress tolerance and brassinosteroid signaling in tomato. Plant Physiol Biochem 127:553-560 https://doi.org/10.1016/j.plaphy.2018.04.031
  17. Lee J, Kim H, Park SG, Hwang H, Yoo Si, Bae W, Kim E, Kim J, Lee HY, Heo TY (2021) Brassinosteroid-BZR1/2-WAT1 module determines the high level of auxin signalling in vascular cambium during wood formation. New Phytologist 230(4):1503-1516 https://doi.org/10.1111/nph.17265
  18. Li J, Liu S, Wang J, Li J, Liu D, Li J, Gao W (2016) Fungal elicitors enhance ginsenosides biosynthesis, expression of functional genes as well as signal molecules accumulation in adventitious roots of Panax ginseng C. A. Mey. J Biotechnol 239:106-114 https://doi.org/10.1016/j.jbiotec.2016.10.011
  19. Love MI, Huber W, Anders S (2014) Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome biology 15(12):1-21
  20. Rahimi S, Kim YJ, Sukweenadhi J, Zhang D, Yang DC (2016) PgLOX6 encoding a lipoxygenase contributes to jasmonic acid biosynthesis and ginsenoside production in Panax ginseng. J Exp Bot 67(21):6007-6019 https://doi.org/10.1093/jxb/erw358
  21. Ratan ZA, Haidere MF, Hong YH, Park SH, Lee JO, Lee J, Cho JY (2021) Pharmacological potential of ginseng and its major component ginsenosides. J Ginseng Res 45(2):199-210 https://doi.org/10.1016/j.jgr.2020.02.004
  22. Subramanian A, Tamayo P, Mootha VK, Mukherjee S, Ebert BL, Gillette MA, Paulovich A, Pomeroy SL, Golub TR, Lander ES (2005) Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles. Proceedings of the National Academy of Sciences 102 (43):15545-15550 https://doi.org/10.1073/pnas.0506580102
  23. Wang C, Liu J, Deng J, Wang J, Weng W, Chu H, Meng Q (2020) Advances in the chemistry, pharmacological diversity, and metabolism of 20(R)-ginseng saponins. J Ginseng Res 44 (1):14-23 https://doi.org/10.1016/j.jgr.2019.01.005
  24. Zhang Y, J in T, Ryu G, Gao Y (2021) Effects of screw configuration on chemical properties and ginsenosides content of extruded ginseng. Food Sci Nutr 9(1):251-260 https://doi.org/10.1002/fsn3.1991
  25. Zhao S, Wang L, Liu L, Liang Y, Sun Y, Wu J (2014) Both the mevalonate and the non-mevalonate pathways are involved in ginsenoside biosynthesis. Plant Cell Rep 33(3):393-400 https://doi.org/10.1007/s00299-013-1538-7