• KSBB Journal
• /
• 제13권5호
• /
• pp.577-584
• /
• 1998

A sensitive membrane strip assay for plasma lipoprotein cholesterol that can be performed without handling reagents has been investigated. We previously developed an assay system with immobilized enzymes (cholesterol esterase and cholesterol oxidase) on the surfaces of nitrocellulose membrane(1). In such a case, the amount of enzymes present on the membrane was limited by its surface area and, thus, the detection capability was relatively poor (> 50 mg/dL cholesterol). To overcome this problem, we devised a new system with non-immobilized enzymes by placing them within interstitial spaces of a celullose membrane pad in a dry state. Upon contact with sample medium, the enzymes were immediately dissolved and participated in the reactions with cholesterol in a liquid phase. We constructed a user-friendly system consisting of four membrane pads fro sample application, cholesterol decomposition, color development as signal, and medium absorption to invoke a continuous flow (sequential location from the bottom). A sample containing lipoproteins was added into the application pad by capillary action and transferred to the next pad for decomposition. The decomposition pad (namely, enzyme pad) contained a detergent (sodium cholate) for the destruction of lipoprotein particles, the two enzymes for cholesterol decomposition, and a chromogen (3,3'-diaminobenzidine). As a consequence of the enzyme reactions, hydrogen peroxide was produced, and then reacted in the presence of the chromogen with horseradish peroxidase immobilized on the signal generation pad. Finally, a colorimetric signal directly proportional to the cholesterol concentration was produced. The detection limit determined from this system under optimal conditions was at least 2 times lower than of the enzyme-immobilized system.

• 비파괴검사학회지
• /
• 제19권5호
• /
• pp.369-377
• /
• 1999

본 연구는 다중박막(multi-layer) 구조 모델에 대한 초음파신호처리 적용으로 접합경계면의 결함검출에 관한 연구이다. 이를 위해서 먼저 반도체 검사법에 의하여 박리(delamination). 다이 균열(die crack) 기포(void)의 유무를 확인할 수 있었고, 각 접합계면에서의 단위 cm당 결함 오차율을 모집군 25%이하에서 0.003%까지 측정 가능하였다. 또한 초음파 화상처리를 이용하여 결함 판독 프로그램을 위한 각 패키지별 화상을 8단계에서 16단계까지 데이터 베이스화할 수 있었고, 최종 결과 화면에서는 결함정도를 확률로 표현 가능하도록 하였으며 기포의 가능성도 추론해 볼 수 있다. 그리고, 박리검사 프로그램(delamination inspection program)에 의하여 결함의 크기와 결함의 원인을 16단계로 추론하고. S.A.T 장치에 귀환(feedback)시킬 수 있는 매개변수를 찾을 수 있었다. 특히, 반도체 결함추출 알고리즘 개발로 반도체 결함검사자동화의 기틀을 마련하였고, 향후 결함을 세분화하고 다양한 반도체 패키지별로 데이터베이스를 구축한다면, 온라인 상태에서 보다 많은 검사를 수행 할 수 있는 인공지능형 자동검사 시스템 구현이 가능할 수 있도록 하였다.

• 대한전자공학회:학술대회논문집
• /
• 대한전자공학회 2001년도 하계종합학술대회 논문집(4)
• /
• pp.151-154
• /
• 2001

In this Paper, a new harmonic imaging technique is proposed and evaluated experimentally. In the proposed method, a weighted chin signal with a hanning window is transmitted. The RF samples obtained on each array element are individually compressed by correlating with the reference signal defined as the 2nd harmonic (2f0) component of a transmitted chirp signal generated in a square-law system. The proposed method uses the compressed 2f0 component to form an image, for which the crosscorrelation term with f0 component should be suppressed below at least -60dB. After experiment, the 6dB pulse width and peak sidelobe level of the compressed 2f0 component were 0.7us and -60dB, respectively. This result shows that the proposed method can successfully eliminate the f0 component with a single transmit-receive event and therefore is more efficient than the conventional pulse inversion (PI) method in terms of frame rate. We also observed that the 2nd harmonic compont starts to decrease for source pressure higher than 210kPa in water, which implies that SNR of the 2nd harmonic imaging using short pulses cnanot be incresed beyond a certain limit.

• 한국군사과학기술학회지
• /
• 제23권3호
• /
• pp.204-212
• /
• 2020

For general target tracking works by receiving a set of measurements from sensor. However, if the SNR(Signal to Noise Ratio) is low due to small RCS(Radar Cross Section), caused by remote small targets, the target's information can be lost during signal processing. TBD(Track Before Detect) is an algorithm that performs target tracking without threshold for detection. That is, all sensor data is sent to the tracking system, which prevents the loss of the target's information by thresholding the signal intensity. On the other hand, using all sensor data inevitably leads to computational problems that can severely limit the application. In this paper, we propose an iterative Joint Integrated Probabilistic Data Association as a practical target tracking technique suitable for a low SNR multi-target environment with real time operation capability, and verify its performance through simulation studies.

• 한국추진공학회지
• /
• 제13권4호
• /
• pp.36-44
• /
• 2009

광학 선형 패터네이터 및 광학 토모그래피 기법을 사용하여 높은 주위 기체 압력 조건에서 분무단면을 측정하였다. 레이저 직선광이 분무 영역을 지나도록 하여 발생하는 Mie 산란 신호 및 직선광의 투과율을 측정하였으며, 이로부터 Beer-Lambert 법칙 및 수학적 단면 재구성 기법을 이용하여 분무 단면의 감쇠 계수 분포를 재구성하였다. 높은 주위 기체 압력 조건에서 광학적으로 밀한 분무가 발생하며, 그 결과 산란 신호의 감쇠 효과가 크게 증가한다. 따라서 미 산란 신호를 이용하는 광학 선형 패터네이터의 경우 감쇠 효과를 완화하는 데 한계를 보인다. 광학 토모그래피의 경우 분무를 통과하는 레이저광의 투과율 정보만을 이용하여 성공적으로 분무 단면을 재구성하였다.

• Journal of Communications and Networks
• /
• 제14권5호
• /
• pp.481-486
• /
• 2012

Accurate and efficient estimation of the number of sources is critical for providing the parameter of targets in problems of array signal processing and blind source separation among other such problems. When conventional estimators work in unfavorable scenarios, e.g., at low signal-to-noise ratio (SNR), with a small number of snapshots, or for sources with a different strength, it is challenging to maintain good performance. In this paper, the detection limit of the minimum description length (MDL) estimator and the signal strength required for reliable detection are first discussed. Though a comparison, we analyze the reason that performances of classical estimators deteriorate completely in unfavorable scenarios. After discussing the limiting distribution of eigenvalues of the sample covariance matrix, we propose a new approach for estimating the number of sources which is based on a sequential hypothesis test. The new estimator performs better in unfavorable scenarios and is consistent in the traditional asymptotic sense. Finally, numerical evaluations indicate that the proposed estimator performs well when compared with other traditional estimators at low SNR and in the finite sample size case, especially when weak signals are superimposed on the strong signals.

• 전기학회논문지
• /
• 제65권8호
• /
• pp.1436-1441
• /
• 2016

Currently, partial discharge diagnosis in the field of prevention applied technology and diagnostic equipment is a possible strong limit to remove the noise generated by external or internal I still have one unreliable diagnosis. This technology is the noise removal from signal the time lag analysis algorithms technique is applied by a fundamental. Increasing the reliability in terms of technology spectrum frequence of analysis method for by applying the acquisition through the position of the frequency content and sources of traffic lights partial discharge of the acquisition of signal analysis to judge whether a way diagnosis the environment of the scene, and conditions. Partial discharge signal and make the discharge while building blocks were found through the Analysis. Spectrum frequence of Analysis and wide discharge part, to be more precise, in line with the various functions, including the analysis technique band. Diagnosis and comes up with advanced technology that can detect the presence of a position. This method is portable single device developed for maintenance and mobility and ease and convenience of getting caught by discharge of the pattern analysis and position detection method suitable for a new diagnosis will suggest.

• 한국식품영양학회지
• /
• 제10권2호
• /
• pp.180-185
• /
• 1997

찹쌀 20%, 엿기름 4%를 가하여 7시간 동안 당화시켜 제조한 식혜는 말통스 10.1%, 한계덱스트린 7.3%, 말토트링스 1.3%, 글루코오스 0.18%, 밥알 1.75%를 나타냈다. 알코올 침전, Biogel P-2의 겔 크로마토그래피로 식혜의 한계덱스트린을 정제하여 1H-NMR 분석한 결과 한계덱스트린은 $\alpha$-1,4-글루코시드 결합과 $\alpha$-1,6-글루코시드 결합이 5:1로 이루어졌고, pullulanase 처리한 결과 말토오스와 말토헥사오스까지의 분포를 나타내어 멥쌀식혜에서 얻은 한계덱스트린가 구조가 같은 결과이다. 밥알의 당함량은 26.4%, 단백질 함량은 41.6%를 나타냈다. 참쌀식혜의 한계덱스트린과 밥알에 30unit/ml의 $\alpha$-아밀라아제, 글\ulcorner아밀라아제, $\alpha$-글루코시다아제, $\beta$-아밀라아제를 작용시킨 결과 글루코아밀라아제 외에는 일부밖에 가수분해하지 at하였다. 인체의 효소인 $\alpha$-아밀라아제와 $\alpha$-글루코시다아제를 함께 작용시킨 결과 한계덱스트린의 가수분해율은 18%, 밥알의 가수분해율은 26%를 나타냈다.

• Bulletin of the Korean Chemical Society
• /
• 제32권6호
• /
• pp.2003-2007
• /
• 2011

We report a highly sensitive surface-enhanced Raman scattering (SERS) platform for the selective trace analysis of mercury (II) ions in drinkable water using aptamer-conjugated silver nanoparticles. Here, an aptamer designed to specifically bind to $Hg^{2+}$ ions in aqueous solution was labelled with a TAMRA moiety at the 5' end and used as a Raman reporter. Polyamine spermine tetrahydrochloride (spermine) was used to promote surface adsorption of the aptamer probes onto the silver nanoparticles. When $Hg^{2+}$ ions are added to the system, binding of $Hg^{2+}$ with T-T pairs results in a conformational rearrangement of the aptamer to form a hairpin structure. As a result of the reduced of electrostatic repulsion between silver nanoparticles, aggregation of silver nanoparticles occurs, and the SERS signal is significantly increased upon the addition of $Hg^{2+}$ ions. Under optimized assay conditions, the concentration limit of detection was estimated to be 5 nM, and this satisfies a limit of detection below the EPA defined limit of 10 nM in drinkable water.

• 한국식품영양학회지
• /
• 제10권3호
• /
• pp.360-364
• /
• 1997

멥쌀식혜에 Saccharomyces cerevisae를 가해 28$^{\circ}C$에서 10일간 발효시켜서 식혜주를 제조하였다. TLC 및 HPLC 분석 결과 발효에 따라 글루코오스는 하루, 말토오스는 4일, 말토트리오스는 6일까지만 존재하였다. 그러나, 분자량이 큰 말토올리고당과 한계덱스트린은 발효되지 않았다. 에탄올은 6.5%를 나타냈다. 식혜주의 아미노산 함량은 2.9$\mu$mol/ml, 단백질 함량은 457$\mu\textrm{g}$/ml를 나타냈다. pH는 3.67, 산도는 3.1ml를 나타냈다. 한계덱스트린은 1H-NMR로 분석 결과 식혜에 존재하는 것과 구조상 변화가 없었다. $\alpha$-1,4-결합에 대한 $\alpha$-1,6-결합의 비율도 5.6:1을 나타냈다. 관능검사 결과, 맛은 와인과 비슷하였다.