• 한국초지조사료학회지
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• 제38권2호
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• pp.99-105
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• 2018

This study was carried out to estimate the effect of selected inoculants on chemical compositions and fermentation characteristics of rye silage. Rye was harvested at dough stage and divided into 5 treatments, following: No additives (CON); L. plantarum R48-27 (LP27); L. buchneri R4-26 (LB26); Mixture of LP27 and LB26 at 1:1 ratio (MIX); and L. buchneri (LB). The rye forage was ensiled into 10 L bucket silo for 100 days. The contents of NDF and ADF were lowest (P<0.05) in LB26. The pH in LB26, MIX, and LB were lower (P<0.05) than CON and LP27. Lactate content in LB was higher (P<0.05) than the others, while acetate content in LB26 and LB were higher (P<0.05) than that in CON and LP27. Lactate to acetate ratio was highest (P<0.05) in LB, but lowest in LB26. Lactic acid bacteria (LAB) count in LB was higher (P<0.05) than that in CON, while yeast count in CON was lower than in all silages applied inoculants. In conclusion, silages inoculated with LB26 could improve potentially the aerobic stability caused by increases of acetate and propionate concentrations.

• 전기학회논문지
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• 제67권7호
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• pp.976-980
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• 2018

The traction power system is configured to transmit electricity to the vehicles through mechanical contact between the OCL (Overhead Contact Line) and the pantograph. The system measures the current collection performance of the OCL, or the OCL installation condition is examined through maintenance for commercial operation. Maintenance continues to check the conditions through visual inspection by walking and inspection vehicles. The current collection performance is divided into the percentage of arcing(%), the contact force, and the uplift. The percentage of arcing is composed of a vision based system and used to verify the performance of a new OCL. However, it is not always possible to measure the current collection performance during commercial operation, and maintenance based on human resources can not be replaced. This paper presents the minimum performance condition of video devices in the current collection system of commercial vehicles. In addition, a continuous arcing was measured, and current collection performance was examined on the traction power system at the 250 km/h. It was analyzed with a minimum duration of arc of 1 ms. The frame rate is then shown by comparing the number of frames in the image at the time intervals of the number of the arcing. It is expected that the result of this study can be used for examining the minimum performance of video devices depending on their purpose.

• 분석과학
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• 제11권4호
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• pp.305-310
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• 1998

아민기를 함유한 Eriochrome Black T 유사체 염료에 cynauric chloride를 반응시켜 합성한 반응성 염료를 셀룰로오스 막(cellulose membrane)에 화학적으로 고정시켜서 2가 전이금속을 검출할 수 있는 광센서를 구성하였다. 이 광센서는 용액상 Eriochrome Black T와 마찬가지로 전이금속의 농도에 민감하게 감응한다. 이 광센서는 대표적으로 $Zn^{2+}$와 $Co^{2+}$에 대해서 각각 $6.3{\times}10^{-5}mol/l$와 $2.5{\times}10^{-4}mol/l$의 검출한계를 나타냈다. 이 광센서는 또한 흐름용액의 pH가 계속해서 변하는 조건하에서도 상당 시간 동안 그 감응성이 감소하지 않았다.

• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.217-223
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• 2010

To evaluate the antiallergic effect of fermented Ixeris sonchifolia (IS, family Compositae), we prepared IS kimchi, isolated lactic acid bacteria (LAB) from it, fermented IS with these LAB, and investigated their antiallergic effects. IS kimchi inhibited the passive cutaneous anaphylaxis (PCA) reaction induced by an IgE-antigen complex as well as the scratching behavior induced by compound 48/80 or histamine more potently than IS. When IS was fermented with LAB isolated from IS kimchi, its antiallergic effects was also increased. Of LAB used for fermentation, Lactobacillus brevis more potently increased the antiallergic effects. Its main constituents, chlorogenic acid and luteolin, potently inhibited the PCA reaction induced by the IgE-antigen complex as well as the pruritis induced by compound 48/80 or histamine. These constituents inhibited the expression of pro inflammatory and allergic cytokines, TNF-$\alpha$. and IL-4, and transcription factor NF-${\kappa}B$ activation induced by the IgE-antigen complex in RBL-2H3 cells, as well as the degranulation of RBL-2H3 cells induced by the IgE-antigen complex. Luteolin more potently inhibited these allergic reactions than chlorogenic acid. These findings suggest that the antiallergic effect of IS can be increased by LAB fermentation, and the fermented IS might improve allergic reactions such as pruritus, anaphylaxis, and inflammation.

• 대한치과기공학회지
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• 제40권1호
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• pp.33-40
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• 2018

Purpose: The purpose of this study was to investigate the influence of mechanical properties of various ultra-dental stone by setting methods. Methods: 240 cylinder specimens($10mm{\times}20mm$) were prepared from three ultra-dental stones(Gemma, Die keen and Fuji rock; n = 80) in accordance with the manufacturers' recommendations. Half of the specimens of each stone(n = 40) were dried in open air within a room temperature; the other half(n = 40) underwent in a silicone rubber mold in open air for 30 minutes and then were dried in a microwave oven for 10 minutes to 600W. Compressive strength(CS), compressive modulus(CM) and diametral tensile strength(DTS) conducted until fracture using Instron 5966 at each of the following periods: 1 and 24 hours from mixing. One-way analysis of variance and Scheffe's post hoc test were performed for statistical comparisons at a significance level of P<.05. Results: The CS and CM values in all dental stone indicated highest after 24h(54.25 MPa < ) than the values for specimens dried in microwave method. The DTS values revealed the highest microwave method. However, in 24h, FJ(Fu-ji rock) and GM(Gemma) had lower mechanical properties than air. Conclusion : Within the limitations of this study, CS did not influence by microwave method but DTS affected according to the setting.

• Bulletin of the Korean Chemical Society
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• 제29권2호
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• pp.417-421
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• 2008

The efficient synthesis of four mesitylene-based receptors 1-4 and their potentiometric response characteristics to alkali metal, alkaline earth metal, and transition metal ions, under various pH conditions are outlined. Receptor 1-based electrode exhibited more sensitive response to Ag+ ion (49 mV/decade of range from 10-6 to 10-2 M) than the 2-based electrode (47 mV/decade of range from 3 ´ 10-5 to 10-2 M), while the 3- and 4-based ones revealed sub-Nernstian below 40 mV/pAg+. All electrodes showed substantial responses to Ag+ ion under acidic condition, but there was almost nil-response to other transition metal ions (Fe2+, Co2+, Zn2+, Ni2+, Pb2+, Cd2+, Cu2+ and Hg2+). The association constant of receptor 1 toward Ag+ ion, measured by 1H NMR titration, showed the largest value (200 M-1) among the tested receptors. The results were interpreted with semi empirically-modeled structures.

• 한국축산식품학회지
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• 제18권2호
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• pp.115-124
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• 1998

This study was carried out investigate hygienic conditions of domestic packing plant for export and the shelf-life by Japanese standards. vacuum packaged chilled port loins provided by five packers(A, B, C, D and E) were transported to Japan in the same condition, Four packers(A, B, C adn d) produced the samples as hygienic as possible and one packer produced loins in the traditional hygienic condition, In TTC(2,3,5-triphenyl tetrazolium chloride) test all samples showed the negative reaction from the date of the quarantine(14 days after slaughtering) to 50 days The numbers of total plate count(TPC) of A, B, C and D packers that were below 108CFU/g until 50 days were accepted by Japanese Standards but TPC of E packer was over 107CFU/g at 30 days. VBN values increased sig-nificantly(P<0.01) in samples of A,B and D packers in the samples of C packer and E packer at 5% and 0.1% level respectively. The pH values of the vacuum packaged chilled pork for export at 14 days after slaughtering were 5.43∼6.00 In sensory evaluation, A, B, C and D packers which improved hygi-enic condition produced the products with good color and appearance. low drip until 40 days storage at $0^{\circ}C$ But as the storage time increase off-flavor occured at 40days in A and D packer and at 50days in B and C packer. In conclusion according to the above results of microbiological physicochemical and sensory evalution the edible periods of Korean vacuum package chilled porks may be estimated to 40∼50 days for the packers who improved hygienic condition and the shelf lives were calculated as 32∼40 days from edible period by a factor of 0.8(safety coefficient) while that of the pork from E packer which was produced in the traditional hygienic condition was estimated to 30 days after slaughtering at $0^{\circ}C$ Therefore the shelf-life of the pork of E packer would be only 24 days.

• 전기화학회지
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• 제7권4호
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• pp.206-210
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• 2004

금속산화물 $(TiO_2,\;RuO_2,\;PbO_2,\;Ni(OH)_2)$과 Prussian blue (PB)를 각각 탄소반죽에 혼합한 후 스크린 프린팅 기법으로 6종의 탄소반죽 전극들을 제작하였다. 제작된 탄소반죽 전극들로 전자혀 시스템을 제작하여 다양한 음료수와 식품에 대한 감응을 0.1M carbonate buffer, pH 9.6완충 용액에 묽힌 후 대시간전류법의 방법으로 측정하였다 얻어진 자료를 주성분 분석법 (principal component analysis; PCA)으로 처리한 후 식음료의 맛을 평가할 수 있도록 2차원 좌표계에 표시하였으며, 그 결과 본 실험에서 제작한 시스템 및 분석법은 다양한 식음료의 종류를 뚜렷이 구분해 낼 수 있음을 확인하였다.

• Bulletin of the Korean Chemical Society
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• 제30권4호
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• pp.897-901
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• 2009

Heme oxygenase-1 (HO-1) is an anti-inflammatory and anti-apoptotic protein and has been applied to various gene therapy researches. However, constitutive expression of HO-1 may induce deleterious side effects. In this research, hypoxia inducible HO-1 expression plasmid, pEpo-SV-HO-1, was constructed with the erythropoietin (epo) enhancer and simian virus 40 (SV40) promoter to avoid these unwanted side effects. Dexamethasone conjugated polyethylenimine (PEI-Dexa) was used as a gene carrier. It was previously reported that dexamethasone protected cardiomyocytes from apoptosis under hypoxia. In this research, PEI-Dexa reduced the caspase-3 level in hypoxic H9C2 cardiomyocytes as a derivative of dexamethasone, suggesting that PEI-Dexa is an anti-apoptotic reagent as well as a gene carrier. pEpo-SV-HO-1 was transfected to H9C2 cardiomyocytes using PEI-Dexa and the cells were incubated under normoxia or hypoxia. HO-1 expression was induced in the pEpo-SV-HO-1 transfected cells under hypoxia. In addition, cell viability under hypoxia was higher in the pEpo-SV-HO-1 transfected cells than the pEpo-SV-Luc transfected cells. Also, caspase-3 level was reduced in the pEpo-SV-HO-1 transfected cells under hypoxia. In addition to the anti-apoptotic effect of PEI-Dexa, hypoxia inducible HO-1 expression by pEpo-SVHO- 1 may be helpful to protect cardiomyocytes under hypoxia. Therefore, pEpo-SV-HO-1/PEI-Dexa complex may be useful for ischemic heart disease gene therapy.

• 폴리머
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• 제37권1호
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• pp.94-99
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• 2013

폴리디아세틸렌(polydiacetylene, PDA)은 자기조립된 디아세틸렌(diacetylene) 단량체의 광중합에 의해 만들어진다. 디아세틸렌 단량체들이 조직적으로 배열되면 254 nm의 자외선 노광에 의해 1,4-첨가 중합이 일어나 고분자 주사슬에 이중결합과 삼중결합이 교대로 존재하는 폴리디아세틸렌이 만들어진다. 폴리디아세틸렌 수용액은 일반적으로 약 640 nm에서 최대흡수파장을 지니는 청색을 띠게 되며 여기에 온도나 pH의 변화, 다른 물질의 결합 등 외부 자극에 의해 약 550 nm의 최대 흡수 파장을 띠는 적색으로 색 전이가 일어나게 된다. 본 연구에서, 우리는 고체상 펩티드 합성을 이용하여 PCDA(10,12-pentacosadyinoic acid) 리포좀의 표면에 양이온성 올리고펩티드를 도입하였다. 또한 다양한 몰 비율로 리포좀 수용액을 제조하여 동물 세포에 트랜스펙션한 결과, 향상된 유전자 전달 효율과 낮은 독성을 보이는 것을 확인하였고, PCDA의 특성을 이용하여 세포에 처리 후 세포 관련 비표지 형광을 관찰하였다.