• Title/Summary/Keyword: short columns

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Comparison of Total RNA Isolation Methods for Analysis of Immune-Related microRNAs in Market Milks

  • Oh, Sangnam;Park, Mi Ri;Son, Seok Jun;Kim, Younghoon
    • Food Science of Animal Resources
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    • v.35 no.4
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    • pp.459-465
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    • 2015
  • Bovine milk provides essential nutrients, including immunologically important molecules, as the primary source of nutrition to newborns. Recent studies showed that RNAs from bovine milk contain immune-related microRNAs (miRNA) that regulate various immune systems. To evaluate the biological and immunological activity of miRNAs from milk products, isolation methods need to be established. Six methods for extracting total RNAs from bovine colostrums were adopted to evaluate the isolating efficiency and expression of miRNAs. Total RNA from milk was presented in formulation of small RNAs, rather than ribosomal RNAs. Column-combined phenol isolating methods showed high recovery of total RNAs, especially the commercial columns for biofluid samples, which demonstrated outstanding efficiency for recovering miRNAs. We also evaluated the quantity of five immune-related miRNAs (miR-93, miR-106a, miR-155, miR-181a, miR-451) in milk processed by temperature treatments including low temperature for long time (LTLT, 63℃ for 30 min)-, high temperature for short time (HTST, 75℃ for 15 s)-, and ultra heat treatment (UHT, 120-130℃ for 0.5-4 s). All targeted miRNAs had significantly reduced levels in processed milks compared to colostrum and raw mature milk. Interestingly, the amount of immune-related miRNAs from HTST milk was more resistant than those of LTLT and UHT milks. Our present study examined defined methods of RNA isolation and quantification of immune-specific miRNAs from small volumes of milk for use in further analysis.

Progressive collapse of steel-framed gravity buildings under parametric fires

  • Jiang, Jian;Cai, Wenyu;Li, Guo-Qiang;Chen, Wei;Ye, Jihong
    • Steel and Composite Structures
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    • v.36 no.4
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    • pp.383-398
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    • 2020
  • This paper investigates the progressive collapse behavior of 3D steel-framed gravity buildings under fires with a cooling phase. The effect of fire protections and bracing systems on whether, how, and when a gravity building collapses is studied. It is found that whether a building collapses or not depends on the duration of the heating phase, and it may withstand a "short-hot" fire, but collapses under a mild fire or a "long-cool" fire. The collapse time can be conservatively determined by the time when the temperature of steel columns reaches a critical temperature of 550 ℃. It is also found that the application of a higher level of fire protection may prevent the collapse of a building, but may also lead to its collapse in the cooling phase due to the delayed temperature increment in the heated members. The tensile membrane action in a heated slab can be resisted by a tensile ring around its perimeter or by tensile yielding lines extended to the edge of the frame. It is recommended for practical design that hat bracing systems should be arranged on the whole top floor, and a combination of perimeter and internal vertical bracing systems be used to mitigate the fire-induced collapse of gravity buildings. It is also suggested that beam-to-column connections should be designed to resist high tensile forces (up to yielding force) during the cooling phase of a fire.

Strength and Moment-Curvature Relationship of HCFT Columns under Eccentric Load (편심압축이 작용하는 HCFT기둥에 대한 내력특성과 모멘트-곡률의 곤계)

  • 이승조;박정민;김화중
    • Journal of the Korea Concrete Institute
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    • v.14 no.6
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    • pp.864-873
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    • 2002
  • Recently, CFT Column has reported a lot of study result, because a CFT column has certain superior structural properties as well as good productivity, execution efficiency, and improved rigidity over existing column. However, CFT column still has problems clearing the capacity evaluation between its steel tube member and high-strength concrete materials. Also, high-strength concrete filled steel square tube column(HCFT) examined numerical value explanatorily about transformation performance(M-ø) of when short-column receives equal flexure-moment from axial stress on research for concrete. hnd, with basis assumption, executed development of analysis program of moment-curvature relation for analytic analysis of transformation performance of HCFT section that get by an experiment. This study investigated to properties of structural(capacity, curvature), through a series of experiments for HCFT with key parameters, such as strength of concrete(600kgf/$\textrm{cm}^2$), D/t ratio, slenderness ratio(λ) and concrete kinds under eccentric load. And, I executed comparative analysis with AISC-LRFD, AIJ and Takanori Sato etc. and experiment result that is capacity design formula.

Numerical experimentation for the optimal design for reinforced concrete rectangular combined footings

  • Velazquez-Santilla, Francisco;Luevanos-Rojas, Arnulfo;Lopez-Chavarria, Sandra;Medina-Elizondo, Manuel;Sandoval-Rivas, Ricardo
    • Advances in Computational Design
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    • v.3 no.1
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    • pp.49-69
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    • 2018
  • This paper shows an optimal design for reinforced concrete rectangular combined footings based on a criterion of minimum cost. The classical design method for reinforced concrete rectangular combined footings is: First, a dimension is proposed that should comply with the allowable stresses (Minimum stress should be equal or greater than zero, and maximum stress must be equal or less than the allowable capacity withstand by the soil); subsequently, the effective depth is obtained due to the maximum moment and this effective depth is checked against the bending shear and the punching shear until, it complies with these conditions, and then the steel reinforcement is obtained, but this is not guaranteed that obtained cost is a minimum cost. A numerical experimentation shows the model capability to estimate the minimum cost design of the materials used for a rectangular combined footing that supports two columns under an axial load and moments in two directions at each column in accordance to the building code requirements for structural concrete and commentary (ACI 318S-14). Numerical experimentation is developed by modifying the values of the rectangular combined footing to from "d" (Effective depth), "b" (Short dimension), "a" (Greater dimension), "${\rho}_{P1}$" (Ratio of reinforcement steel under column 1), "${\rho}_{P2}$" (Ratio of reinforcement steel under column 2), "${\rho}_{yLB}$" (Ratio of longitudinal reinforcement steel in the bottom), "${\rho}_{yLT}$" (Ratio of longitudinal reinforcement steel at the top). Results show that the optimal design is more economical and more precise with respect to the classical design. Therefore, the optimal design presented in this paper should be used to obtain the minimum cost design for reinforced concrete rectangular combined footings.

A Study on the Architectural Design Characteristics of the Plan and the Structure in Sudeok Temple's Daeung-Jean (건축설계 측면에서 본 수덕사 대웅전의 평면과 가구 특성에 관한 연구)

  • Kim, Do-Kyoung
    • Journal of architectural history
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    • v.17 no.4
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    • pp.97-112
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    • 2008
  • In this study, I attempted to the architectural design characteristics of Daeung-Jeon in Sudeok Temple. For this purpose, After I set up several assumptions in the basis of the general characteristics of Korean wood architecture, and then, analyzed floor plan, structural formation and section sizes of structure and bracket members in relation to module and unit. As the results, the characteristics of the design process of plan and structure are follows. (1) 1 ja(尺), the unit applied to this building is measured $307.6{\sim}318.3mm$) and the average is 312.9mm (2) It is estimated that the floor plan designed on the basis of the top of columns. By the applied unit, every bay of the front side and the side is each designed by 15 ja and 8.5 ja. (3) The section is composed of piled members which have same section size. As basic module of section size called 'jae(재;材)', it is estimated at width 0.45 ja by height 0.75 ja. And as the secondary module, height between jae and is called 'gyoe(계;)' and it wes designed by three height size of 0.25 ja, 0.27 ja and 0.30 ja, (4) It is estimated that the section plan was designed by the order as follows. Firstly, the horizontal position of purlins wes decided on the basis of the intersection point of long and short rafters, and then the position and the section size of purlins and jangheyo(長舌) wes decided on the basis of the slope of roof and rafters. Secondly, going down from purlins, the members of structure composed of 'jae' and 'gyoe' was repeated. Lastly, for the purpose of linking the structure members located on the center line of adjacent purlins organically, the height of whaban(화반) was controlled.

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Seismic analysis of half-through steel truss arch bridge considering superstructure

  • Li, Ruiqi;Yuan, Xinzhe;Yuan, Wancheng;Dang, Xinzhi;Shen, Guoyu
    • Structural Engineering and Mechanics
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    • v.59 no.3
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    • pp.387-401
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    • 2016
  • This paper takes a half-through steel truss arch bridge as an example. A seismic analysis is conducted with nonlinear finite element method. Contrast models are established to discuss the effect of simplified method for main girder on the accuracy of the result. The influence of seismic wave direction and wave-passage on seismic behaviors are analysed as well as the superstructure and arch ring interaction which is mostly related with the supported bearings and wind resistant springs. In the end, the application of cable-sliding aseismic devices is discussed to put forward a layout principle. The main conclusions include: (1) The seismic response isn't too distinctive with the simplified method of main girder. Generally speaking, the grillage method is recommended. (2) Under seismic input from different directions, arch foot is usually the mostly dangerous section. (3) Vertical wave input and horizontal wave-passage greatly influence the seismic responses of arch ring, significantly increasing that of midspan. (4) The superstructure interaction has an obvious impact on the seismic performance. Half-through arch bridges with long spandrel columns fixed has a less response than those with short ones fixed. And a large stiffness of wind resistant spring makes the the seismic responses of arch ring larger. (5) A good isolation effectiveness for half-through arch bridge can be achieved by a reasonable arrangement of CSFABs.

The Study on the Jointing Method of Wooden Members at Unified Silla Architecture (통일신라건축 목조결구기법에 관한 연구)

  • Hwang, Se-Ok;Hur, Bum-Pal
    • Journal of architectural history
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    • v.18 no.1
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    • pp.7-29
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    • 2009
  • In understranding the essence of the Korea traditional Architecture, it is important to consider the jointing methods of architectural members, architectural technologies, etc. Especially the purpose of this study is understanding on the Jointing Method of Wooden Members in the period of Unified Silla Architecture. It's conclusion is summarized as follows. 1. A section of column has very close to do with the foundation stone. The structures of foundation stone and column are generally concluded by butt joint, arrow-head joint, housed joint by Grang-e method. Judu is structured by arrow-head joint And, in general, beam is structured by Sagaematchum Chumcha and sagaljudu of Don direction. At the head of Pyungju and the body of Goju, Changbang is structed by Jangbumachum with arrow-head joint or by jumukchang-machum. Also, it is surmised that Gyisoseum and Anssolim methods had been applied to columns from former ages. The example can be found at Bagjae Mireuksaji stone pagoda. Bagjae Mireuksaji stone pagoda taking wooden-pagoda form adopts Gyisoseum and Anssolim methods. We can also find such a sort of methods from other stone constructions like Budo, etc. 2. Injahwaban is structured by short Changbumachum with arrowed-head joint at upper members, and by Anjangmachum at the lower part. This sort of Gongpo style can be seen in the mural painting of tomb of Koguryo and in Buplyungsa, Buplyunsa, Bupkisa-located in Japan, which are influenced by Bakjae or Unified Silla. It is considered that at the end of the late United Silla, Injawhaban had been replaced with Chumcha and Soro on the Pyungbang under influence of Dapo style from China.

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Non-destructive Inspection of Construction Joints of Concrete Structures Using the Radar and the Infrared Thermography Method (레이더법과 적외선법을 이용한 콘크리트 시공 이음부 공극의 비파괴검사)

  • Park, Seok-Kyun
    • Journal of the Korea Concrete Institute
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    • v.15 no.3
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    • pp.425-432
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    • 2003
  • The joint treatment of concrete is one of the technical problems in concrete constructions. Joints created with concrete constructions result in serious weakness in the aspects of both structural and water-barrier function. The radar and the infrared thermography method have been used for the non-destructive inspection of several construction joints of concrete structures in this study. The advantages and limitations of these methods are investigated for non-destructive inspection on construction joints of concrete columns. It can be shown that the detecting precision of construction joints using these methods is improved if radar analysis is carried out with a simulation analysis. In case of the infrared thermography method, the shape of construction joints can be also detected when heating is performed before testing. As the result, it has been verified that the construction joints, difficult to be detected by visual inspection, could be inspected effectively in broad areas at short period of time when these two methods are applied.

Investigation of three-dimensional deformation mechanisms of box culvert due to adjacent deep basement excavation in clays

  • Bu, Fanmin;Yu, Wenrui;Chen, Li;Wu, Erlu
    • Geomechanics and Engineering
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    • v.30 no.6
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    • pp.565-577
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    • 2022
  • In this study, a series of three-dimensional numerical parametric study was conducted to investigate deformation mechanisms of an existing box culvert due to an adjacent multi-propped basement excavation in clays. Field measurements from an excavation case history are first used to calibrate a baseline Hardening Soil Small Strain (HS-small) model, which is subsequently adopted for parametric study. Results indicate that the basement-box culvert interaction along the basement centerline can be considered as a plane strain condition when the length of excavation (L) reaches 14 He (i.e., final excavation depth). If a plane strain condition (i.e., L/He=12.0) is assumed for analyzing the basement-box culvert interaction of a short excavation (i.e., L/He=2.0), the maximum settlement and horizontal movement of the box culvert are overestimated significantly by up to 15.7 and 5.1 times, respectively. It is also found that the deformation of box culvert can be greatly affected by the basement excavation if the distance between the box culvert and retaining wall is less than 1.5 He. The induced deformation in the box culvert can be dramatically reduced by improving the ground inside the excavation or implementing other precautionary measures. For example, by adding jet grouting columns within the basement and installing an isolation wall behind the retaining structures, the maximum settlements of box culvert are shown to reduce by 37.2% and 13.4%, respectively.

Case Study on Economical Fabrication and Erection of Steel Structure and Reduction in Field Erection Time (경제적 철골제작$\cdot$설치 및 공기단축 사례분석연구)

  • Ahn Jae-Bong;Choi Yoon ki
    • Korean Journal of Construction Engineering and Management
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    • v.5 no.5 s.21
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    • pp.183-192
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    • 2004
  • Even in Korea the number of steel structure buildings that allow internal space and easy change of their layouts in accordance with the purpose of buildings and box-type steel bridges constructed with thick plates with thickness in a rage just from a few $\beta$AE to \$100\beta$AE is increasing these days and therefore, domestic fabrication and processing technology of members for steel structures is being improved at a pace faster than in the past to meet the growing requirements of consumers for high reliability on quality control on the related steel structures. However, most domestic fabricators os steel structures who are turning out their steel products in accordance with the designs prepared by engineering companies in their respective works for the sake of cost cut more than anything else, hesitating to introduce any advanced new technology into themselves. In the case of the steel structure design application for small and mid-size buildings in particular, it is quite meaningful not only for those who are involved in steel structure business, but also for the people working at construction work fields to review the result of the study on the connections of steel structure members deigned to obtain superb quality of steel structures within short period for steel fabrication and erection at fields in economical ways, as there is a glowing tendency seeking standardization of connection of steel structure members as well as whole structure together with the development on design of construction system of buildings including their exterior and interior decoration materials, manufacture of the related members and fabrication technique structure. This paper has been prepared with the aim to review the peculiar characteristics of buildings constructed with the main frames of steel structures and actual cases of the change made ing the connections between steel structure columns and between columns and girder members in order to reduce the work period necessary for fabrication and erection of steel structures at the maximum as well as the some examples of steel structures fabricated through automatic welding by robots for box-type columns in addition to the description of the problems found in the course of fabricating those steel structures, suggesting possible counter-measures to solve them.