• Journal of the Korean Wood Science and Technology
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• 제49권4호
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• pp.384-393
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• 2021

Recently, due to environmental and toxicity issues, there has been increasing attention on research regarding natural products that can reduce the use of chemical fertilizers. Wood extracts derived from the biorefining process contain various fertilizer ingredients. HPLC analysis revealed that wood extract contains approximately 5.2% hemicellulosic sugar. The growth of lettuce (Lactuca sativa) upon treatment with wood extract (extract obtained from steam-exploded pine) or water-soluble fertilizers containing different nutrients was analyzed in this study. After two weeks, the growth characteristics of lettuce as affected by wood extract or water-soluble fertilizers were significantly different. The effect of water-soluble fertilizers containing ascorbic acid, magnesium sulfate, citric acid, potassium nitrate, amino acids, or seaweed extract was less desirable than that of wood extracts regarding plant height (18.6 cm), number of leaves (10), leaf length (14.1 cm), shoot fresh wight (9.8 g/plant), root fresh weight (0.8 g/plant) and shoot dry weight (0.6 g/plant). The plant height, number of leaves, leaf length, shoot fresh wight, root fresh weight, shoot dry weight of water-soluble fertilizers containing wood extract were significantly different compared to the control (plant height :13.5 cm, number of leaves : 7, leaf length : 9.4 cm, shoot fresh wight : 5.3 g/plant, root fresh weight : 0.7 g/plant, shoot dry weight : 0.4 g/plant, root dry weight : 0.07 g/plant). From these results, it was concluded that wood extract can be used as a potential water-soluble fertilizer to increase the yield of leafy vegetables.

• Journal of Plant Biotechnology
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• 제37권4호
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• pp.505-510
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• 2010

This study was conducted to refine a micropropagation method of water hyacinth (Eichhornia crassipes) in vitro. When young shoots were cultured on media with various concentrations of BA or TDZ alone, LS medium containing $5.0\;mgl^{-1}$ BA was found favorable for shoot proliferation from young shoots with a mean of 4.2 shoots. Using BA together with IAA, more shoots were obtained on LS medium containing $5.0\;mgl^{-1}$ BA and $1.0\;mgl^{-1}$ IAA with a mean of 5.7 shoots. In liquid medium, number of shoots and fresh weight per explant increased significantly. The best shoot proliferation and increasing of fresh weight were achieved on LS liquid medium containing $5.0\;mgl^{-1}$ BA and $1.0\;mgl^{-1}$ IAA with 6.9 shoots and more than 4,000 mg fresh weight. Of the different concentrations of LS salt, double strength of LS medium provided the highest shoot proliferation with 7.3 shoots, and fresh weight with 5,539 mg per explant. Shoot proliferation on LS medium containing $50\;gl^{-1}$ sucrose had better results with 8.7 shoots and 5,979 mg per explant in fresh weight than other conditions. In conclusion, the optimal level for shoot proliferation and biomass increase of water hyacinth was attained with the application of the double strength of LS medium containing $5.0\;mgl^{-1}$ BA, $1.0\;mgl^{-1}$ IAA and $50\;gl^{-1}$ sucrose.

• 한국환경농학회지
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• 제29권4호
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• pp.408-416
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• 2010

To evaluate the effectiveness of AMF on the growth of horticultural crops, we compared mycorrhizal and non-mycorrhizal plants, perilla (P. frutescens Britt.), that were inoculated with AMF propagules. In the early stages of growth of perilla, compared to the AMF- perilla seedlings, in AMF+ perilla seedlings at 3 weeks after sowing, leaf length and width increased 17% and 29%, leaf area increased 28%, and shoot fresh weight increased 33%, root total length increased 1%, and chlorophyll content increased 3%. Further at 10 weeks after sowing, compared to the AMF- perilla plants, in perilla plants inoculated with AMF at the sowing and transplanting stages, leaf area increased 21% and 19%, shoot length increased 19% and 17%, root fresh weight increased 17% and 20%, and chlorophyll content increased 5.1% and 4.8%, respectively. Moreover, at 14 weeks after sowing, compared to the AMFperilla plants, in perilla plants inoculated with AMF at the sowing and transplanting stages, the number of leaves increased 16% and 20%, root fresh weight increased 16% and 17% significantly. Further, leaf fresh weight increased 9% and 11%, shoot diameter increased 4.5% and 7.3%, and chlorophyll content increased 1.5% and 2.5%, respectively. The levels of many macronutrients and micronutrients were tended to be significantly higher in AMF+ plants than in AMF- plants, supporting the association between AMF and enhanced growth of plants grown from AMF+ seedlings.

• International Journal of Industrial Entomology and Biomaterials
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• 제4권2호
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• pp.143-147
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• 2002

Studies on the interrelationship between biological yield and harvest index, and their association with shoot rearing were carried out in 9 promising mulberry genotypes, which indicated significant variations among genotypes. Biological yield had close association with leaf yield components indicating that they are inter-related for higher productivity in mulberry, Biological yield revealed highly significant positive correlation with important leaf yield components. However height of the longest branch showed negative correlation with biological yield. Genotypes with higher biological yield associated with low ratio of stem weight to leaf weight and high Harvest index on dry weight basis are to be selected for shoot rearing. Biological yield recorded on fresh weight basis had less impact on the present study in comparison to dry weight basis.

• 한국산림과학회지
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• 제96권2호
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• pp.178-182
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• 2007

Rauvolfia serpentina (L.) Benth. ex Kurz is a medicinal plant and an endangered tropical rainforest plant species. Since the field cultivation that aims to fulfill the industrial needs is never accomplished, tissue culture appears to be the most feasible way to improve the quality and quantity of R. serpentina. This experiment used two kinds of explants (roots and shoots) to induce optimal root formation in different combinations of auxin and photoperiod. Each explants exhibited different responses on given treatments. Differentiated root could be produced from explants cultured in IBA 20 mg/L with and without light. The highest number of roots, root length and root weight induced from shoot explants were effective on MS medium containing IBA 20 mg/L and incubated under dark condition, while highest total weight (callus and root) from root explants cultured on MS medium supplemented 10 mg/L IBA and 10 mg/L NAA and incubated under day length (11/13 hr). The root induced from shoot explants produced the highest major alkaloid content. The highest content of ajmaline (2.17 ppm fresh weight) and reserpine (1.30 ppm fresh weight) were observed in shoot explants cultured in MS medium containing combination of IBA 10 mg/L and NAA 10 mg/L and incubated under dark condition, yohimbine (1.47 ppm fresh weight) was in the shoot explants cultured in MS medium containing NAA 20 mg/L and incubated under day length, while serpentine was absent.

• 생물환경조절학회지
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• 제17권1호
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• pp.60-65
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• 2008

돌나물의 노지 가을삽식을 위하여, 줄기의 삽수 부위(상부, 중부, 하부) 및 삽식 방법(점파, 조파 산파)에 따른 월동전후 생육특성을 조사하였다. 삽수부위에 따른 삽목후 40일째의 월동전 생육은 상부 삽수에서 초장, 근장, 줄기수, 지경수, 지상부 생체중 및 건물중 등에서 유의하게 높았으며, 월동후 개화전(May 10th) 생육은 개체당 줄기수, $m^2$당 지상부 생체중 및 건물중에서 하부 삽수보다 유의한 증가를 보였다. 삽식 방법에 따른 삽목후 40일째의 월동전 생육은 점파($10{\times}10cm$)가 조파나 산파보다 양호하였고, $m^2$당 지상부 생체중 및 건물중도 점파>산파>조파 순으로 높았다. 월동후 생육(May 10th)은 개체당 줄기수, $m^2$당 생체중 및 건물중에서 점파가 조파보다 유의하게 증가하였다. 돌나물의 가을 노지 삽식은 줄기 상부의 삽수를 이용하는 것이 월동 전후 생육 및 수량성에 유리하였으며, 삽식방법은 점파에서의 수량성이 가장 양호하였으나, 생력화를 위해서는 월동후 생체중에서 점파와 유의한 차이를 보이지 않은 혼파방법이 바람직할 것으로 기대되었다.

• 한국작물학회지
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• 제26권1호
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• pp.115-120
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• 1981

토양수분이 인삼생육에 미치는 영향을 조사하기 위하여 토경Pot(1/2000cm)에 묘삼을 이식하여 토양수분함량별 지상부 및 지하부 생육장황, 근수량과 지상부 형질과의 상호관계를 조사하여 다음과 같은 결과를 얻었다. 1. 인삼생육에 대한 토양의 적정수분 함량은 포장용수량의 65. 5%(절대수분 22.1%였고 생육이 정지되는 토양수분 함량은 포장용수량의 31.5%(절대수분 10.7%)였다. 2. 토양수분 함중이 적은 곳에서는 출아 및 전엽율이 상당히 지연되었으며 엽면적외 감소와 지상부 결주가 많아져 근수량이 현저히 감소되였다. 3. 근수량은 엽면적(r=0.91**) 경직경(r=0.76**) 경장(r=0.71**) 지상부생중(r=0.96**)간에 각각 정의 상기관계가 있었고 지상부 결주율(r=-0.77**) 간에 부의 상관관계가 있었다. 4. 생근중과 수분함량(r=- 0.80**)간에 부의 상관관계가 있어 수분이 충분한 곳에서 자란 인삼은 조직이 치밀함을 나타내었다.

• 농업과학연구
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• 제44권2호
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• pp.196-210
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• 2017

The synergistic effect on phosphate solubilization of single- and co-inoculation of two phosphate solubilizing bacteria, Burkholderia cepacia (C1) and Alcaligenes aquatilis (H6), was assessed in liquid medium and maize plants. Co-inoculation of two strains was found to release the highest content of soluble phosphorus (309.66 ?g/mL) into the medium, followed by single inoculation of B. cepacia (305.49 ?g/mL) and A. aquatilis strain (282.38 ?g/mL). Based on a plant growth promotion bioassay, co-inoculated maize seedlings showed significant increases in shoot height (75%), shoot fresh weight (93.10%), shoot dry weight (84.99%), root maximum length (55.95%), root fresh weight (66.66%), root dry weight (275%), and maximum leaf length (81.53%), compared to the uninoculated control. In a field experiment, co-inoculated maize seedlings showed significant increases in cob length (136.92%), number of grain/cob (46.68%), and grain weight (67.46%) over control. In addition, single inoculation of maize seedlings also showed improved result over control. However, there was no significant difference between single inoculation of either bacterial strains and co-inoculation of these two bacterial strains in terms of phosphate solubilization index, phosphorous release, pH of the media, and plant growth parameters. Thus, single inoculation and co-inoculation of these bacteria could be used as biofertilizer for improving maize growth and yield.

• 식물조직배양학회지
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• 제25권1호
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• pp.63-68
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• 1998

정단 및 마디조직을 이용한 지황의 기내증식방안을 마련하기 위하여 일련의 시험을 실시하였다. 재생된 신초의 정단을 재배양하였을 경우 BA 5.0 mg/L에서 7.8개의 신초가 형성되었으며 투명화된 묘의 발생은 Gelrite 보다는 Bacto-agar 첨가구에서 감소하였다. 광도와 한천의 농도가 증가할수록 신초형성수 및 생장은 억제되었고 생체중 : 건물중의 비율도 감소하였다. 광도가 2000 lx일 때는 Bacto-agar 0.6-1.2%, 3,000 lx에서는 한천 0.4-0.6%에서 건전한 신초를 10개 이상 생산할 수 있었다. 활성탄소 0.1-0.3%첨가는 신초의 생장촉진과 뿌리형성에 촉진적으로 작용하였으며 투명화 발생률도 감소시켰으나 신초형성수는 현저히 감소시켰다. MS배지내 첨가되는 다량원소의 농도를 0.8-1.0배 하였을때 신초형성수 및 생장이 촉진되었다. 기관형성에 미치는 오옥신의 종류 및 농도별 효과를 조사한 결과 NAA나 IBA 보다는 IAA 0.3mg/L 와 BA 5.0mg/L를 혼합처리 하였을 때 증식률이 16배에 달하였다.

• 생물환경조절학회지
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• 제16권3호
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• pp.240-246
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• 2007

돌나물의 수확후 저장온도가 저장성에 미치는 영향을 분석하기 위하여 저장온도에 따른 생체중, 색상차, 엽록소 함량, 수분함량, 외관상 변화 등을 조사하였다. 생체중은 저장온도에 따라 8.7-25.3%의 감소를 보였으며, 저장온도가 높을수록 생체중 감소율도 높았다. 계통에 따른 생체중 감소는 포항지방종이 가장 컷으며, 완주 지방종의 변화가 비교적 적었다. 외관상 품질은 $20^{\circ}C$에서 4일경, $10^{\circ}C$에서 6일경, $5^{\circ}C$에서 8일경부터 하위 잎에 변색이 나타나기 시작하였으며, 시간이 지남에 따라 서서히 부패하기 시작하였다 SPAD 값으로 비교한 엽록소 함량은 $20^{\circ}C$와 $10^{\circ}C$에서는 4일과 6일 후부터 급격한 감소를 보였으나, $5^{\circ}C$에서는 변화가 적었다. 잎의 색도 변화에서 명도를 나타내는 L값과 녹색-적색을 나타내는 a값은 저장온도가 높을수록 저장일 수에 따라 유의하게 증가하였다. 저장 후 10일째에 변색이나 부패한 돌나물의 비율은 저장온도가 높을수록 증가하였으며, 이는 수확시의 수분함량과 유의한 상관 관계를 보였다(P=0.01). 계통간에는 완주 지방종이 비교적 저장성이 높았으며, 군산, 완도, 포항 순으로 부패율이 높았다. 따라서 돌나물을 스치로폼 접시에 랩포장 할 경우, $5^{\circ}C$에서는 수확 후 8일까지 상품성을 유지한 상태로 저장이 가능하였다.