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Co-inoculation of Burkholderia cepacia and Alcaligenes aquatilis enhances plant growth of maize (Zea mays) under green house and field condition

  • Received : 2017.04.06
  • Accepted : 2017.06.02
  • Published : 2017.06.30

Abstract

The synergistic effect on phosphate solubilization of single- and co-inoculation of two phosphate solubilizing bacteria, Burkholderia cepacia (C1) and Alcaligenes aquatilis (H6), was assessed in liquid medium and maize plants. Co-inoculation of two strains was found to release the highest content of soluble phosphorus (309.66 ?g/mL) into the medium, followed by single inoculation of B. cepacia (305.49 ?g/mL) and A. aquatilis strain (282.38 ?g/mL). Based on a plant growth promotion bioassay, co-inoculated maize seedlings showed significant increases in shoot height (75%), shoot fresh weight (93.10%), shoot dry weight (84.99%), root maximum length (55.95%), root fresh weight (66.66%), root dry weight (275%), and maximum leaf length (81.53%), compared to the uninoculated control. In a field experiment, co-inoculated maize seedlings showed significant increases in cob length (136.92%), number of grain/cob (46.68%), and grain weight (67.46%) over control. In addition, single inoculation of maize seedlings also showed improved result over control. However, there was no significant difference between single inoculation of either bacterial strains and co-inoculation of these two bacterial strains in terms of phosphate solubilization index, phosphorous release, pH of the media, and plant growth parameters. Thus, single inoculation and co-inoculation of these bacteria could be used as biofertilizer for improving maize growth and yield.

Keywords

References

  1. Afzal A, Bano A. 2008. Rhizobium and phosphate solubilizing bacteria improve the yield and phosphorus uptake in wheat (Triticumaestivum). International Journal of Agriculture and Biology10:85-88.
  2. Ali B, Sabri A, Hasnain S. 2010. Rhizobacterial potential to alter auxin content and growth of Vignaradiata (L.) World Journal of Microbiology and Biotechnology 26:1379-1384. https://doi.org/10.1007/s11274-010-0310-1
  3. Boronin АM. 1998. Rhizosphere bacteria of the genus Pseudomonas enabling plant growth and development. Sorovsky Educational Magazine 10:25-31.
  4. Chen YP, Rekha PD, Arun AB, Shen FT, Lai WA, Young CC. 2006. Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Applied Soil Ecology34:33-41. https://doi.org/10.1016/j.apsoil.2005.12.002
  5. Chen Z, Ma S, Liu LL. 2008. Studies on phosphorus solubilizing activity of a strain of phospho bacteria isolated from chestnut type soil in China. Bioresource Technology 99:6702-6707. https://doi.org/10.1016/j.biortech.2007.03.064
  6. Chung H, Park M, Madhaiyan M, Seshadri S, Song J, Cho H, Sa T. 2005. Isolation and characterization of phosphate solubilizing bacteria from the rhizosphere of crop plants of Korea. Soil Biology and Biochemistry 37:1970-1974. https://doi.org/10.1016/j.soilbio.2005.02.025
  7. Das S, Lyla PS, Khan SA. 2007. Biogeochemical processes in the continental slope of Bay of Bengal: I. Bacterial solubilization of inorganic phosphate. Revista de Biología Tropical 55:1-9.
  8. Edi-Premono, Moawad MA,Vleck PLG. 1996. Effect of phosphate solubilizing Pseudomonas putida on the growth of maize and its survival in the rhizosphere. Indonesian Journal of Crop Sciences 11:13-23.
  9. Gaur AC. 1990.Phosphate solubilizing microorganisms as biofertilizers. Omega Scientific Publishers, New Delhi, India.
  10. Goenadi DH, Sisweto I, Sugiarto Y. 2000. Bioactivation of poorly soluble phosphate rocks with a phosphorussolubilizing fungus. Soil Science Society of America Journal 64:927-932.
  11. Halder AK, Chakrabartty PK. 1993. Solubilization of inorganic phosphate by Rhizobium. Folia Microbiologica 38:325-330. https://doi.org/10.1007/BF02898602
  12. Hamid AAA, Hamdan S, Ariffin SHZ, Huyop F. 2010. Molecular prediction of dehalogenase producing microorganism using 16S rDNA analysis of 2,2-dichloropropionate (dalapon) degrading bacterium isolated from volcanic soil. Journal of Biological Science 10:190-199. https://doi.org/10.3923/jbs.2010.190.199
  13. Hariprasad P, Niranjana SR. 2009. Isolation and characterization of phosphate solubilizing rhizobacteria to improve plant health of tomato. Plant and Soil 316:13-24. https://doi.org/10.1007/s11104-008-9754-6
  14. Harman GE.1992. Development and benefits of rhizosphere competent fungi for biological control of plant pathogens. Journal of Plant Nutrition 15:835-843. https://doi.org/10.1080/01904169209364366
  15. Hou YH, Wang QF, Shen JH, Miao JL, Li GY. 2008. Molecular identification of a halotolerant bacterium NJ82 from Antarctic sea ice and preliminary study on its salt tolerance. Microbiology 35:486-490.
  16. Jones DL. 1998. Organic acids in the rhizosphere-A critical review. Plant and Soil 205:25-44. https://doi.org/10.1023/A:1004356007312
  17. Khalid A, Arshad M, Zahir ZA. 2004. Screening plant growth promoting rhizobacteria for improving growth and yield of wheat. Journal of Applied Microbiology 96:473-480. https://doi.org/10.1046/j.1365-2672.2003.02161.x
  18. Khalimi K, Suprapta DN, Nitta Y. 2012. Effect of Pantoea agglomerans on growth promotion and yield of rice. Agricultural Science Research Journals 2:240-249.
  19. Kimura M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16:111-120. https://doi.org/10.1007/BF01731581
  20. Linu MS, Stephen J, Jisha MS. 2009.Phosphate solubilizing Gluconacetobacter sp., Burkholdia sp. and their potential interaction with cowpea (Vignaunguiculata (L.). Intern. Journal of Agricultural Research 4:79-87.
  21. Maliha R, Samina K, Najma A, Sadia A, Farooq L. 2004. Organic acids production and phosphate solubilization by phosphate solubilizing microorganisms under in vitro conditions. Pakistan Journal of Biological Sciences 7:187-196. https://doi.org/10.3923/pjbs.2004.187.196
  22. Paul D, Sinha SN. 2013a. Isolation of phosphate solubilizing bacteria and total heterotrophic bacteria from river water and study of phosphatase activity of phosphate solubilizing bacteria. Advances in Applied Science Research 4:409-412.
  23. Paul D, Sinha SN. 2013b. Phosphate solubilization potential and phosphatase activity of some bacterial strains isolated from thermal power plant effluent exposed water of river. Ganga CIB Tech Journal of Microbiology 2:1-7.
  24. Perez E, Sulbarn M, Maria Ball MM, Yarzabal LA. 2007. Isolation and characterization of mineral phosphatesolubilizing bacteria naturally colonizing a limonitic crust in the south-eastern Venezuelan region. Soil Biology & Biochemistry 39:2905-2914. https://doi.org/10.1016/j.soilbio.2007.06.017
  25. Pikovskaya RI. 1948. Mobilization of phosphorous in soil in the connection with vital activity of some microbial species. Mikorobiologiya 17:362-370.
  26. Podile AR, Kishore GK. 2006. Plant growth-promoting rhizobacteria. In Plant-associated bacteriaedited by Gnanamanickam SS. pp. 195-230. Springer, Netherlands.
  27. Pundarikakshudu R. 1989. Studies of the phosphate dynamics in a vertisol in relation to the yield and nutrient uptake of rainfed cotton. Experimental Agriculture 25:39-45. https://doi.org/10.1017/S0014479700016422
  28. Rangarajan S, Saleena LM, Vasudevan P, Nair S. 2003. Biological suppression of rice disease by Pseudomonas sp. under saline soil condition. Plant Soil 251:73-82. https://doi.org/10.1023/A:1022950811520
  29. Rodriguez H, Fraga R. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances 17:319-339. https://doi.org/10.1016/S0734-9750(99)00014-2
  30. Sharma SB, Sayyed RZ, Trivedi MH, Gobi TA. 2013. Phosphate solubilizing microbes: Sustainable approach for managing phosphorus deficiency in agricultural soils. SpringerPlus 2:587. https://doi.org/10.1186/2193-1801-2-587
  31. Subba Rao NS.1982. Phosphate solubilization by soil microorganisms. Advances in Agricultural Microbiology. pp.1-149. Oxford & IBH Publishing Co.
  32. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30:2725-2729. https://doi.org/10.1093/molbev/mst197
  33. Tao GC, Tian SJ, Cai MY, Xie GH. 2008. Phosphate-solubilizing and -mineralizing abilities of bacteria isolated from soils. Pedosphere18:515-523. https://doi.org/10.1016/S1002-0160(08)60042-9
  34. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. 1997. The CLUSTAL_X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25:4876-4882. https://doi.org/10.1093/nar/25.24.4876
  35. Vessey K. 2003. Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil 255:571-586. https://doi.org/10.1023/A:1026037216893
  36. Von der Weid I, Paiva E, Nobrega A, van Elsas JD, Seldin L. 2000. Diversity of Paenibacillus polymyxa strains isolated from the rhizosphere of maize planted in Cerrado soil. Research in Microbiology 151:369-381. https://doi.org/10.1016/S0923-2508(00)00160-1
  37. Whitelaw MA. 2000. Growth promotion of plants inoculated with phosphate solubilizing fungi. Advances in Agronomy 69:99-151.
  38. Xiang WL, Liang HZ, Liu S, Luo F, Tang J, Li MY, Che ZM. 2011. Isolation and performance evaluation of halotolerant phosphate solubilizing bacteria from the rhizospheric soils of historic Dagong Brine Well in China. World Journal of Microbiology and Biotechnology 27:2629-2637. https://doi.org/10.1007/s11274-011-0736-0
  39. Yu X, Liu X, Zhu TH, Liu GH, Mao C. 2011. Isolation and characterization of phosphate-solubilizing bacteria from walnut and their effect on growth and phosphorus mobilization. Biology and Fertility of Soils 47:437-446. https://doi.org/10.1007/s00374-011-0548-2
  40. Zaidi A, Khan MS, Ahemad M, Oves M, Wani PA. 2009. Recent advances in plant growth promotion by phosphatesolubilizing microbes. pp. 23-50. Springer-Verlag.
  41. Zhang Z, Schwartz S, Wagner L, Miller W. 2000. A greedy algorithm for aligning DNA sequences. Journal of Computational Biology 7:203-214. https://doi.org/10.1089/10665270050081478

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