• Proceedings of the Korean Society of Applied Pharmacology
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• 1998.11a
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• pp.176-176
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• 1998

An enhanced formation of nitric oxide (NO) is an important mediator of hypotention, peripheral vasodilation and vascular hyporeactivity to vasoconstrictor agents in endotoxaemia. And tumor necrosis factor (TNF${\alpha}$), as a primary mediator of circulatory shock has been known to induce inducible nitric oxide synthase (i-NOS), leading to excessive production of NO. We isolated two sesquiterpene lactone compounds from Saussurea lappa and their structures were elucidated as dehydrocostus lactone and costunolide. These compounds inhibit the production of both NO and TNF${\alpha}$ by LPS (1 $\mu\textrm{g}$/$m\ell$)-activated Raw 264.7 cells. NO was measured spectropho-tometrically as nitrite by the Griess reagent and TNF${\alpha}$ by ELISA. Dehydrocostus lactone (IC$\sub$50/ : 3.0 ${\mu}$M) and costunolide (IC$\sub$50/ : 4.5 ${\mu}$M) inhibited the production of NO in LPS-activated Raw 264.7 cells by suppressing inducible nitric oxide synthase enzyme expression. These compounds also decreased the TNF${\alpha}$ levels in LPS-activated system in vitro and in vivo.

• The Bulletin of The Korean Astronomical Society
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• v.44 no.2
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• pp.47.2-47.2
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• 2019

Observations indicate that turbulence in molecular clouds of the interstellar medium (ISM) is highly supersonic (M >> 1) and strongly magnetized (β ≈ 0.1), while in the intracluster medium (ICM) it is subsonic (M <~1) and weakly magnetized (β ≈ 100). Here, M is the turbulent Mach number and β is the ratio of the gas to magnetic pressures. Although magnetohydrodynamic (MHD) turbulence in such environments has been previously studied through numerical simulations, some of its properties as well as its consequences are not yet fully described. In this talk, we report a study of MHD turbulence in molecular clouds and the ICM using a newly developed code based the high-order accurate, WENO (Weighted Essentially Non-Oscillatory) scheme. The simulation results using the WENO code are generally in agreement with those presented in the previous studies with, for instance, a TVD code (Porter et al. 2015 &, Park & Ryu 2019), but reveal more detailed structures on small scales. We here present and compare the properties of simulated turbulences with WENO and TVD codes, such as the spatial distribution of density, the density probability distribution functions, and the power spectra of kinetic and magnetic energies. We also describe the populations of MHD shocks and the energy dissipation at the shocks. Finally, we discuss the implications of this study on star formation processes in the ISM and shock dissipation in the ICM.

• The Korean Journal of Physiology and Pharmacology
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• v.28 no.4
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• pp.379-387
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• 2024

Breast cancer (BC) is most commonly diagnosed worldwide. Liquiritigenin is a flavonoid found in various species of the Glycyrrhiza genus, showing anti-tumor activity. This article was to explore the influences of liquiritigenin on the biological behaviors of BC cells and its underlying mechanism. BC cells were treated with liquiritigenin alone or transfected with oe-HSP90 before liquiritigenin treatment. RT-qPCR and Western blotting were employed to examine the levels of HSP90, Snail, E-cadherin, HSC70, and LAMP-2A. Cell viability, proliferation, migration, and invasion were evaluated by performing MTT, colony formation, scratch, and Transwell assays, respectively. Liquiritigenin treatment reduced HSP90 and Snail levels and enhanced E-cadherin expression as well as inhibiting the proliferation, migration, and invasion of BC cells. Moreover, liquiritigenin treatment decreased the expression of HSC70 and LAMP-2A, proteins related to chaperone-mediated autophagy (CMA). HSP90 overexpression promoted the CMA, invasion, and migration of BC cells under liquiritigenin treatment. Liquiritigenin inhibits HSP90-mediated CMA, thereby suppressing BC cell growth.

• Journal of Ginseng Culture
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• v.2
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• pp.39-70
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• 2020

In this paper, through the mutual interpretation and verification of the ancient Korean history books with different origin that have been suspected as false documents, it proves that they could be logically real records and reveal that the substance of the legendary 'medicine for anti-aging and longevity', which also had been mentioned in Chinese old books, is Korean ginseng. Furthermore, with reference to the modern Y chromosomal map of the migratory routes of mankind corresponding to these routes recorded in 「Budoji」, the core history book, the formation of the four ethnic constitution groups (Sasang Constitution) based on the life style of each human group has been estimated. And the cause of Korean ginseng with fever problem for Southeast Asians is their pharmacogenomic constitution problem by protopanaxatriol (PPT) type ginsenosides in ginseng. It was resolved with over production of protopanaxdiol (PPD) type ginsenosides against PPT type in Korean red ginseng as historical or scientific point of view. In addition, by explaining that the processing method to Korean red ginseng could increase red ginseng acidic polysaccharides (RGAP), the RGAP, PPD type ginsenosides, and arginine which is originally abundant in Korean ginseng could increase the expression of the 'heat shock proteins' as a kind of chaperone in the body, this paper presents the theory allowing the scientific interpretation of the efficacy of Korean red ginseng as an 'adaptogen' or 'medicine for anti-aging and longevity'. Lastly, through the consideration of the growing environment of American ginseng and Korean ginseng, the differences are presented.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.243-243
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• 1994

Although active systemic anaphylaxis and passive cutaneous anaphylaxis have been empolyed to study anaphylactic hypersensitivity, it is difficult and time-consuming to induce these reactions in experimental animals. In recent, Jun et al have found a simple method to induced anaphylactic hypersensitivity such as anaphylactic shock(AS) and cutaneous reaction(CR) using compound48/80. Cortex mori (Morus alba L.), the root bark of mulberry tree has been used as an antiphlogistic, diuretic, and expectorant in herbal medicine. The purpose of this study was to determine whether the methanol extract of Cortex mori could inhibit the compound 48/80-induced AS and CR. To induce AS, various doses of compound 48/80 (5, 7.5, 10, 15$\mu\textrm{g}$/gm B.W.) were injected intraperitoneally (i.p.) into ICR mice. The animals were pretreated by three injection(i.p.) of Cortex mori before compound 48/80 administration. Peripheral blood was collected from the right ventricle to estimate the level of serum histamine at 15 minutes after the injctin(i.p.) of various concentration of compound48/80. Mortility rate, mean death time and mesenteric mast cell degranulation rate were evaluated over a 72 hour period. To estimate the effect of Cortex mori on compound 48/80-induced cutaneous reaction, various doses of compound 48/80 with or without Cortex mori were injected intradermally(i.d.) into the shaved flank of Sprague-Dawley rats, and the blue cutaneous patchs induced by Evans'blue injection at the compound 48/80 alone and Cortex mori plus compound 48/80 injection sites were observed. As a Parameter of these reactions, the levels of histamine in the supernatant, calcium uptake and intracellular CAMP of RPMC were measured. supernatant, 1)compound 48/80-induced mortility rate, mean death time, mesenteric mast cell degranulation rate, and serum histamine level in ICR mice were significantly inhibited by pretreatment of Cortex mori, 2) cutaneous reaction inducd by compound48/80 was well developed in Sprague-Dawley rat, but Cortex mori inhibited the compound 48/80-induced blue patch formation remarkably, 3) the compound 48/80-induced degranulation, histamine release and calcium uptake of RPMC pretreated with Cortex mori were significantly inhibited, compared to those of control without Cortex mori pretreatment, and 4)the level of cAMP of RPMC was reduced bythe increased concentration of compound 48/80, pretreatment of Cortex mori not only inhibited the compound 48/80-induced reduction of CAMP but also significantly increased the level of cAMP naturally, from the above results, it is suggested that Cortex mori has an some substances with an ability to inhibits the compound 48/80-induced AS,CR, and mast cell activation.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.3
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• pp.424-435
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• 2020

Objective: The study was conducted to investigate variations in the immunophysiological responses to exercise-induced stress in Jeju and Thoroughbred horses. Methods: Blood samples were collected from the jugular veins of adult Jeju (n = 5) and Thoroughbred (n = 5) horses before and after 30 min of exercise. The hematological, biochemical, and immunological profiles of the blood samples were analyzed. Blood smears were stained and observed under a microscope. The concentration of cell-free (cf) DNA in the plasma was determined using real time polymerase chain reaction (PCR). Peripheral blood mononuclear cells (PBMCs) and polymorphonuclear cells were separated using Polymorphprep, and the expression of various stress-related and chemokine receptor genes was measured using reverse transcriptase (RT) and real-time PCR. Results: After exercise, Jeju and Thoroughbred horses displayed stress responses with significantly increased rectal temperatures, cortisol levels, and muscle catabolism-associated metabolites. Red blood cell indices were significantly higher in Thoroughbred horses than in Jeju horses after exercise. In addition, exercise-induced stress triggered the formation of neutrophil extracellular traps (NETs) and reduced platelet counts in Jeju horses but not in Thoroughbred horses. Heat shock protein 72 and heat shock protein family A (Hsp70) member 6 expression is rapidly modulated in response to exercise-induced stress in the PBMCs of Jeju horses. The expression of CXC chemokine receptor 4 in PBMCs was higher in Thoroughbred horses than in Jeju horses after exercise. Conclusion: In summary, the different immunophysiological responses of Jeju and Thoroughbred horses explain the differences in the physiological and anatomical properties of the two breeds. The physiology of Thoroughbred horses makes them suitable for racing as they are less sensitive to exercise-induced stress compared to that of Jeju horses. This study provides a basis for investigating the link between exercise-induced stresses and the physiological alteration of horses. Hence, our findings show that some of assessed parameters could be used to determine the endurance performance of horses.

• Economic and Environmental Geology
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• v.56 no.6
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• pp.629-646
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• 2023

Host rock of Cheonjeon-ri petroglyph is shale belonging to the Daegu Formation of Cretaceous Gyeongsang Supergroup. The rocks were hornfelsified by thermal alteration, and shows high density and hardness. The petroglyph forms weathered zone with certain depth, and has difference in mineral and chemical composition from the unweathered zone. As the physical deterioration evaluations, most of cracks on the surface appear parallel to the bedding, and are concentrated in the upper part with relatively low density. Breakout parts are occurred in the upper and lower parts of the petroglyph, accounting for 6.0% of the total area and occurs to have been created by the wedging action of cracks crossing. The first exfoliation parts occupying the surface were 23.8% of the total area, the second exfoliations covered with 9.3%, and the exfoliation parts with three or more times were calculated as 3.4%. It is interpreted that this is not due to natural weathering, and the thermal shock caused by the cremation custom here in the past. As the ultrasonic properties, the petroglyph indicates highly strength in the horizontal direction parallel to bedding, and the area with little physical damage recorded mean of 4,684 m/s, but the area with severe cracks and exfoliations showed difference from 2,597 to 3,382 m/s on average. Physical deterioration to the Cheonjeon-ri petroglyph occurred to influence by repeated weathering, which caused the rock surface to become more severe than the inside and the binding force of minerals to weaken. Therefore, it can be understood that when greater stress occurs in the weathered zone than in the unweathered zone, the relatively weathered surface loses its support and exfoliation occurs.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.1
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• pp.60.1-60.1
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• 2015

High energy photons and mechanical energy produced by the process of star formation result in copious FIR molecular and atomic lines, which are important coolants of the system. Photons thermally or mechanically induced could dissociate water in the dense envelope to change relative abundances among the species of O, OH, and H2O. Here we analyze OH emission lines toward embedded young stellar objects (YSOs) observed as part of the Herschel open time key program, 'Dust, Ice, and Gas In Time (DIGIT)' in order to study the physical conditions of associated gas and the energy budget loaded on the OH line emission. According to our analysis of the Herschel/PACS spectra, OH emission peaks at the central spaxel in most of sources, but several sources show spatially extended emission structures. In the extended emission sources, the distribution of OH emission is correlated with that of [OI] emission and extended along the outflow directions. Considering the diversity of source properties, ratios between detected OH lines are relatively constant among sources. In addition, each OH line has strong correlation with bolometric luminosity. In order to determine the physical conditions of YSOs, we adopt several methods for the analysis of the OH lines: rotational diagram, non-LTE LVG analysis, and a 2-D PDR code. From the simple LVG analysis, we find that the thermal solution with the dense ( > $10^7cm^{-3}$) and warm ( ~ 100 K) OH gas reproduces the ratios of detected OH lines. However, our self-consistent PDR 2-D model, which can deal with the IR-pumping effect from the central protostar as well as the warm dust in situ, cannot fit the observational results, suggesting that an irradiated shock model is necessary for a better interpretation.

• Journal of the Korean Society of Propulsion Engineers
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• v.16 no.5
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• pp.47-57
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• 2012

A modified ignition and growth(I&G) model which is necessary to simulate the combustion phenomena of energetic materials and an analytical model determining the unknown parameters of the reaction rate equation are proposed. The modified I&G model sustains important physical implications with overcoming some problems of previous rate equations. This rate model consists of ignition term which represents the formation of the hotspot due to void collapse and growth term which means the shock to detonation transition phenomena. Also, the theoretical model is used to investigate the combustion characteristics of certain energetic materials before running Hydrocode by pre-determination of unknown parameter, $b,\;G,\;x,\;I$. The analytical model provides efficient and highly accurate results rather than previous method which simulated the unconfined-rate-stick via the numerical means.

• YAKHAK HOEJI
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• v.51 no.4
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• pp.264-269
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• 2007

Alzheimer’s disease (AD) is characterized pathologically by the presence of intracellular neurofibrillary tangles and deposition of ${\beta}-amyloid $ (A ${\beta}$) peptides, which are generated by processing of amyloid precursor protein (APP). It is urgent to develop effective therapies for the treatment of AD, since our society rapidly accelerate aging. A${\beta}$ peptides have been believed to be neurotoxic and now are also considered to have effects on the mechanism of memory formation. In this study, effects of radicicol on the metabolism of APP were analyzed. Radicicol inhibited the secretion of A${\beta}$ from the Neuro2a cell line (APPswe cell) expressing APPswe. Beta-site APP cleaving enzyme (BACE) fluorescence resonance energy transfer (FRET) assay revealed that it inhibited BACE activity in a dose dependently manner. Immunoblotting study showed that it inhibited intracellular heat shock protein (HSP)90 and it increased the secretion of HSP90 from the APPswe cells. We suggest that radicicol inhibits APP metabolism and Ap generation by the means of HSP90 inhibitory mechanism and partially BACE inhibitory mechanism. This is the first report that radicicol inhibits the secretion of A${\beta}$ peptides from neuroblastoma cells.