• Clinical and Experimental Reproductive Medicine
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• v.14 no.1
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• pp.7-17
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• 1987

It appears that a major determinant of the success of in vitro fertilization is the selection of the optimal follicle containing an oocyte capable of being fertilized and producing a normal pregnancy. However, the hormonal basis of oocyte maturation is not well substantiated by the as yet available informations. It has been suggested that prolactin(PRL) may stimulate the formation of an oocyte maturation inhibitor and thus inhibit the maturation of oocyte. During the hyperstimulated menstrual cycles serum estradiol($E_2$) levels are markedly elevated, and it seems justified to assume that serum prolactin levels may be elevated since estrogens are potent stimulators of prolactin secretion. This study was carried out to ascertain the effect of the elevated serum estradiol levels on the serum prolactin levels in women undergoing ovarian hyperstimulation with either hMG and/or clomiphene citrate. Serum estradiol and prolactin profiles were measured from third menatrual cycle day to ovulation or ovum aspiration day in 11 normal menstruating women and 30 women who underwent an in vitro fertilization procedure with ovarian hyperstimulation by hMG, clomiphene citrate/hMG, clomiphene citrate. Ovum aspiration was performed 36 hours after hCG administration. The day of ovum aspiration or ovulation was designated Day 0. Serum estradiol levels increased progressively during the follicular phase and this rise peaked on Day-1 at a mean concentration of 1,204${\pm}$189.0pg/ml in Group II(hMG), 1,194${\pm}$167.9pg/ml in Group III(clomiphene citrate/hMG), 1,035${\pm}$195.1pg/ml in Group IV(clomiphene citrate) respectively and on Day -2 of 336${\pm}$34.5pg/ml in Group I(normal control). The elevated estradiol levels fen rapidly after ovulation or ovum aspiration. Serum estradiol values of hyperstimulated groups(Group II, III, IV) were significantly higher than that of control group(Group I) from Day -6 to Day +1, but there was no significant difference of estradiol values among the hyperstimulated groups. Serum prolactin levels increased and peaked on Day +1 at a mean concentration of 60.8${\pm}$14.4ng/ml in Group II, 34.2${\pm}$7.0ng/ml in Group III, 30.1${\pm}$5.7ng/ml in Group IV respectively, but no significant elevation was observed in Group I. Levels of estradiol and prolactin can be positively and significantly correlated in the hyperstimulated groups. However, the increase of serum prolactin levels in hMG group was significantly higher than those in clomiphene citrate/hMG or clomiphene citrate group.

• The Korean Journal of Nuclear Medicine
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• v.9 no.2
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• pp.1-11
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• 1975

The serum concentrations of thyrotropin (TSH) were measured by means of radioimmunoassay, in 98 cases of normal controls, 51 cases of hyperthyroidism, 80 cases of primary hypothyroidism and 4 cases of secondary hypothyroidism to evaluate the diagnostic significance in various functional states of the thyroid. The obtained data were analyzed in correlation with other thyroid function test values in various phases of the functional thyroid diseases. The results were as follows: 1) The serum TSH concentration in normal control group was $<1.3{\sim}8.0{\mu}U/ml$. 2) The measurement of serum TSH was more significant in diagnostic accuracy compared with that of serum $T_4(75.0{\pm}12.2%)$. Free $T_4$ Index ($64.2{\pm}15.2%$), serum $T_3(41.0{\pm}21.0%)\;or\;T_3$ resin uptake ($41.1{\pm}15.8%$) in evaluation of primary hypothyroidism. 3) In case of overt hypothyroidism, the serum TSH and $T_4$ were both abnormal, compatible with the clinical diagnosis, while in case of preclinical or mild hypothyroidism, the serum $T_4(41.2{\pm}23.8%)\;or\;50.0{\pm}25.0%)$ was much less reliable than serum TSH. 4) In the treatment of primary hypothyroidism with desiccated thyroid, the administration of 1 grain of the hormone per day was sufficient to suppress the serum concentration of TSH to normal range. It showed that the measurement of serum TSH concentration was a significant criteria in evaluating the efficiency of the treatment of hypothyroidism. 5) The measurement of serum TSH concentration is a very significant method in the early detection of hypothyroidism induced during or after the treatment of the hyperthyroidism with antithyroid drugs or radioactive Iodine ($^{131}I$).

• The Korean Journal of Nuclear Medicine
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• v.3 no.2
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• pp.1-16
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• 1969

The double tracer study on erythrokinetics was carried out experimentally with radioactive iron ($^{59}Fe$) and chromium ($^{51}Cr$) in rabbits. The 0.1% canthalidin solution and 1% pot. perchlomate solution was given subcutaneously to 20 rabbits respectively. 3 and 6 days after injection, the blood chemistry, urine examination, ferrokinetics and apparent half survival time of RBC were ($^{51}Cr\;T\frac{1}{2}$)determined. Following were the results: 1) Red blood cell hematocrit and hemoglobin values were moderately reduced and B.U.N. and serum creatinine values were slight]y inercased in the canthalidin group, while B.U.N. and serum creatinine values were within normal limits in the pot. perchlomate group. Reticulocyte values were slight]y increased in the canthalidin group, while was normal range in the pot. perchlomate group. 2) Blood chemistry finding was not significant statistically in both experimental groups, but serum iron value was moderately reduced in both group. 3) Plasma volume was unchanged in both group, but red cell volume and whole blood volume were slightly reduced in both groups. 4) Results of ferrokinetics were as follows: i) The plasma iron disappearance rate was delayed in both groups. Plasma iron turnover rate, red cell iron utilization and red cell iron turnover rate were decreased in both groups, and then red cell iron turnover rate was more decreased than plasma iron turnover rate in both groups. Circulating red cell iron was slight]y increased in canthalidin group and red cell iron concentration was within normal range in both groups. ii) P.I.T.R.-R.C.I.T. value was moderately increased in the canthalidin group and slightly increased in the pot. perchlomate group. Reticulocyte index, red cell iron turnover index, plasma iron turnover index and effective erythropoiesis index were whole]y reduced in both groups. iii) The red cell life span was slightly shortened in the canthalidin group while was within normal range in pot. perchlomate group. The pathologic finding of renal biopsy of the canthalidin group shows a selective damage in glomerulus, while shows almost normal range or slight damage in tubules. And that of the pot. perchlomate group shows a selective damage in tubules with slight damage of glomerulus.

• YAKHAK HOEJI
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• v.38 no.6
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• pp.756-762
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• 1994

To investigate the acute toxicity of Elfvingia applanata$(P_{ers})K_{ARST}$, it was extracted with hot water. EA, the aqueous extract from the carpophores of E. applanata, was lyophilized and a dark brownish powder was obtained. The acute toxicity of EA was investigated in ICR mice of both sexes. Five groups of mice were administered orally and intraperitoneally with 375, 750, 1,500, 3,000, 6,000 mg/kg(p.o.) and 312.5, 625, 1,250, 2,500, 5,000 mg/kg (i.p.) of EA Abnormal clinical signs related to EA were not observed and no deaths occurred. $LD_{50}$ values administered orally and intraperitoneally were evaluated to be over 6,000 mg/kg and over 5,000 mg/kg, respectively. Gross finding of necropsy revealed no evidence of specific toxicity related to EA. There were no significant changes in body weights, serum biochemical values and histopathological changes compared with control group.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.9
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• pp.4342-4348
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• 2013

The present study was intended to reveal the relationships between serum lipid levels and various factors of obesity and blood pressure. The study subjects were 1,838 adult women measured at a mass health screening during the period from January through December, 2011. TC, TG, HDL-C, LDL-C, SBP, DBP, degree of obesity, body fat rate were measured and the relation between these obesity and blood pressure measurements to serum lipid levels were studied. As a results, TC, TG, LDL-C, body fat rate and degree of obesity increased linearly with advancing age. TC, TG and LDL-C increased linearly with increasing blood pressure, and these values were higher in hypertension group than that of normal group. TC, TG and SBP increased linearly with increasing degree of obesity, and these values were higher in obesity group than that of normal group. HDL-C decreased linearly with increasing degree of obesity, and these values were lower in obesity group than that of normal group. TC, TG, HDL-C, degree of obesity, body fat rate was positive correlation with each others, but these values negatively correlated to HDL-C. Principal component analysis, showed that subjects could be divided into the group having the hypertensive group(1st principal component), the obesity group(2nd principal component), the hyperlipidemia group(3rd principal component), and HDL-C(4th principal component). In multiple regression analysis, age, TC, TG and body fat rate were affected to HDL-C. Above results suggest that higher the degree of obesity and blood pressure, the higher the serum lipid levels.

• The Korean Journal of Community Living Science
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• v.15 no.1
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• pp.77-83
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• 2004

The aim of this study was to investigate the effects of L-carnitine on the components of serum and liver and the effects on the anti-oxidant system. For this purpose, five experimental groups were setup. For fat source, perilla oil enough with unsaturated fatty acid and beeftallow enough with saturated fatty acid were supplemented together with L-carnitine to the rats. Five experimental groups kept eight Sprague-Dawley rats respectively, They were co group supplemented with basic diet or AIN-93, PO group supplemented with perilla oil, POC group supplemented with perilla oil and L-carnitine, BT group supplemented with beeftallow, and BTC group supplemented with beeftallow and L- carnitine. The results are. 1) Weight gain, food intake and FER were not different significantly among the experimental groups. 2) Significant difference was observed in serum total lipid(P<0.05), serum triglyceride(P<0.05), serum total cholesterol (P<0.05)and serum LDL cholesterol(P<0.05). Serum total lipid and serum triglyceride were significantly low in the groups supplemented with L-carnitine. Serum total cholesterol showed difference with the supplementation of L-carnitine in BTC only. LDL cholesterol showed no significant difference with the supplementation of L-carnatine, but total values of LDL-cholesterol were high in groups supplemented with beeftallow. 3) Total cholesterol in liver was low in POC group with the supplementation of L-carnitine however, there was no difference in BTC group with the supplementation of L-carnitine. In summary, dietary L-carnitine did not influence the weight gain, food intake and food efficiency ratio among the experimental groups, but had an effect of lowering the serum total lipid and triglyceride significantly in both groups which were supplemented with L-carnitine. The effect of lowering of sew total cholesterol with the supplementation of L-carnitine in beeftallow group(BTC) only. The effect of lowering of liver total cholesterol with the supplementation of L-carnitine in perilla oil group(POC) only.

• Journal of Nutrition and Health
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• v.35 no.2
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• pp.223-228
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• 2002

The purpose of this study was to investigate the relationship between iron deficiency without anemia and physical performance in healthy women aged 20-21 yrs. Ten subjects with normal iron stores (serum ferritin $\geq$ 12$\mu\textrm{g}$/L: iron-sufficient group) and 11 subjects with iron depletion without anemia (serum ferritin < 12 $\mu\textrm{g}$/L and serum hemoglobin > 120 g/L: iron-depleted group) were chosen from a group of 50 women and were given physical-performance tests, including determinations of maximum oxygen consumption (VO$_2$ max) and ventilatory threshold. Iron status assessment included determination of hemoglobin, hematocrit, seam ferritin, total iron-binding rapacity, serum iron and transferrin saturation values. Dietary iron intake was assessed based on seven-day food intake records written by the subjects. Physical activity level was estimated by frequency questionnaires and two-week physical activity records were compiled daily by the subjects. Blood ferritin concentration was significantly lower in the iron-depleted group than in the iron-sufficient group (p < 0.05). However, other variables showing iron status was not different between the groups. There were no significant differences in body size, body composition and physical activity levels between the groups. Daily dietary iron, total protein and animal protein intakes of the iron-sufficient group were significantly higher than those of the iron depleted group. However, no differences were found in the amount of dietary vitamin C and fiber between the groups. The values for VO$_2$max and VO$_2$max corrected with weight or fat-free mass were not different between the groups. However, the ventilatory threshold was significantly higher in the iron sufficient group than in the iron-depleted group. The lower ventilatory threshold in the iron-depleted group suggests that iron depletion without anemia could diminish aerobic physical performance in young women. In addition, a significant correlation of physical performance to serum fferritin level was shown only in the iron depleted group.

• Korean Journal of Community Nutrition
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• v.15 no.1
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• pp.61-72
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• 2010

The purpose of this study was to evaluate the effect of a weight control program on anthropometric values, serum lipid profiles and nutrient intakes. The subjects of this study were 38 obese children (boy : 17, girl : 21) with obesity index over 120%. The weight control program for obese children included nutritional education (50 min) and exercise (50 min) for 10 weeks. The nutrition intakes of the children were surveyed before and after the weight control program by 24hr recall test. The BMI, R$\ddot{o}$hrer Index, Obesity Index, WHR (Waist - Hip ratio) and body fat(%) were significantly decreased after completion of the weight control program. The total cholesterol and HDL-cholesterol of subjects were significantly increased after the weight control program. Distribution of serum lipid profiles was slightly changed. The energy intakes were significantly decreased (p < 0.001). from 1760.8 kcal to 1435.2 kcal. In addition, the intakes of P, Zn, retinol, vitamin $B_1$, vitamin $B_2$, vitamin E and niacin were significantly decreased. while intakes of vitamin C and folate were increased. Calcium and folic acid were upper 25% of subjects, under EAR(Estimated Average Requirements) intake before the weight control program. The distribution of energy intakes was significantly changed into positive status; fat percentage was decreased 26.3% to 22.1% (p < 0.01). Carbohydrate was increased 58.6% to 61.2% (p < 0.05). Meal distribution of energy intakes was changed; calorie percentage from lunch significantly increased from 32.2% to 38.3%. Calorie percentage from snack significantly decreased from 17.7% to 13.5%. In conclusion, weight control program for 10 weeks is effective in obesity index and nutrient intakes although serum lipid values were a little changed.

• Journal of Nutrition and Health
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• v.29 no.3
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• pp.307-320
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• 1996

The enforcement and its effect of weight control program including nutrition education, exercise and behavioral modification therapy during 20 weeks were studied in primary school obese children. The sample consisted of treatment group(n=19) and control group (n=23). To evaluate the effects of weight control program, treatment group took pretest and posttest for nutrition knowledge, attitude and food habits. Nutrient intakes, serum lipid levels and anthropometric values were also measured. After weight control program, body weight of treatment group significantly decreased from 47.0$\pm$9.0kg to 45.9$\pm$8.5kg(p<0.001), but than of control group increased from 47.8$\pm$8.1kg to 49.0$\pm$8.7kg (p$\leq$0.05). Obesity index showed decline of 8.6% for treatment group and gain of 0.7% for control group. After self-control period, body weight and relate index in both groups were increased. For the nutrition knowledge test results, the posttest mean scores of treatment group showed the significant increase(p<0.001). For the attitude toward nutrition and food habit results, the posttest mean scores increased significantly (p<0.05, p<0.01). There was a decline of 28% in serum triglycerides, whereas serum total cholesterol increased 11% of baseline. These findings suggest that well-combined weight control program for obese children may result in a substantial loss of body weight during 14 weeks. In addition, these reuslts indicate that weight redution through weight control program can be a viable approach to help normalize serum lipids in obese children. Finally, these data suggest that it may be important to identify that affect weight loss and maintenance, and to examine how to manipulate these factors in weight control program.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.9
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• pp.3867-3870
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• 2015

Background: To explore whether combined detection of serum tumor markers (CEA, CA72-4, CA19-9 and TSGF) improve the sensitivity and accuracy in the diagnosis of gastric cancer (GC). Materials and Methods: An automatic chemiluminescence immune analyzer with matched kits were used to determine the levels of serum CEA, CA72-4, CA19-9 and TSGF in 45 patients with gastric cancer (GC group), 40 patients with gastric benign diseases (GBD group) hospitalized in the same period and 30 healthy people undergoing a physical examination. The values of those 4 tumor markers in the diagnosis of gastric cancer was analyzed. Results: The levels of serum CEA, CA72-4, CA19-9 and TSGF of the GC group were higher than those of the GBD group and healthy examined people and the differences were significant (P<0.001). The area under receiver operating characteristic (ROC) curves for single detection of CEA, CA72-4, CA19-9 and TSGF in the diagnosis of GC was 0.833, 0.805, 0.810 and 0.839, respectively. The optimal cutoff values for these 4 indices were 2.36 ng/mL, 3.06 U/mL, 5.72 U/mL and 60.7 U/mL, respectively. With combined detection of tumor markers, the diagnostic power of those 4 indices was best, with an area under the ROC curve of 0.913 (95%CI 0.866~0.985), a sensitivity of 88.9% and a diagnostic accuracy of 90.4%. Conclusions: Combined detection of serum CEA, CA72-4, CA19-9 and TSGF increases the sensitivity and accuracy in diagnosis of GC, so it can be regarded as the important means for early diagnosis.