• Journal of the korean veterinary medical association
• /
• v.20 no.6
• /
• pp.371-376
• /
• 1984

This study was conducted to determine the normal values for chemical components of blood serum were determined or normal 10 males and 10 females of Korean Native Ogolgyes, l82 days. The measured mean values, standard deviation and ranges of blood glucose,

• Bulletin of the Korean Chemical Society
• /
• v.25 no.6
• /
• pp.791-795
• /
• 2004

The binding of cethyl trimethylammonium bromide, (CTAB) with human serum albumin (HSA) has been investigated at 5 mM phosphate buffer pH 7.0, 27 $^{\circ}C$ and various ionic strength using ion selective membrane electrodes. This method is faster and much more accurate than equilibrium dialysis technique, so provides sufficient and accurate data for binding data analysis. A novel and simple method was introduced for resolution and characterization of binding sets on basis of binding capacity concept. The values of Hill binding parameters were estimated for each set and used for calculation of intrinsic binding affinity. The results interpreted on basis of nature of forces which interfered in the interaction and represent the existence of three and two binding sets for binding of CTAB at $10^{-4}$ and $10^{-3}$ M of NaBr, respectively.

• Korean Journal of Veterinary Research
• /
• v.35 no.2
• /
• pp.399-404
• /
• 1995

In order to clarify the significance of serum CPK for the diagnosis of the cardiac disease in ruminant, the fluctuation of serum total CPK activities and CPK isoenzyme fractions was examined before and after operation in Korean native goats with artificially induced cardiac injury. In the change of serum total CPK activities, those of experimental group were changed with higher values than those of control group and significant increase was found on 2-week after operation(P<0.05). In the fluctuation of serum CPK isoenzyme fractions, significant increase of both CK1 and CK3 fractions was observed on 2-week after operation in experimental group(P<0.05), but significant difference was not found in CK2 fraction between experimental and control groups. From these findings, it is considered that taking note of increase of not only serum total CPK activities but also CK1 and CK3 fractions is important for the diagnosis of traumatic cardiac disease in ruminant.

• Korean Journal of Veterinary Pathology
• /
• v.3 no.2
• /
• pp.87-91
• /
• 1999

The present study was undertaken to compare the variation on serum biochemical values by storage in the rats. Sera were prepared from 30 Sprague-Dawley rats of each sex. 5 aliquots from each serum were placed in a -80$^{\circ}C$ freezer with the exception of I aliquots which was analyzed immediately. The analysis was performed on the following months; 1, 2, 3, 6, and 12 months after freezing. The parameters measured) were aspartate aminotransferase(AST), alanine aminotransferase(ALT), alkaline phosphatase(ALP), blood urea nitrogen(BUN) creatinine(CRE), glucose(GLU), total cholesterol(TCHO), triglyceride (TG), total protein(TP), albumin(ALB), total bilirubin(TBIL), calcium(Ca$\^$＋＋/), inorganic phosphorus(IP), creatine kinase (CK), phospholipid(PL), albumin-globulin ratio(A/G), sodium(Na$\^$＋/), potassium(K$\^$＋/), and chloride(Cl$\^$－/) The statistical analysis with Repeated Measures ANOVA, did not show statistical significance in the parameters of AST, ALT, BUN, TG, CK, A/G, Na$\^$＋/ of 1 month freezed sera, in those of AST, TG, CK, K$\^$＋/) of 2 month freezed sera, in those of AST, ALT, BUN, CRE, TCHO, TP, TBIL, CK, PL, Na$\^$＋/), K$\^$＋/), Ct on month fteezed sera, in those of Cl$\^$－/ of 6 month fteezed sera, and in those of ALT, TG, ALB of 12 month freezed sera in male SD rats. On the other hand, it did not show statistical significance in the parameters of AST, ALT, ALP, BUN, GLU, TCHO, TG, TBIL, CK, PL, A/G, Na$\^$＋/ of 1 month freezed sera, in those of AST, TCHO of 2 month freezed sera, in those of AST, BUN, CRE, TCHO, TP, TBIL, CK, PL of 3 month freezed sera, in those of TCHO, IP, PL of 6 month freezed sera, and in those of ALB of 12 month freezed sera in female SD rats. On the basis of the results, although there are some statistical variations in the biochemical values of the sera, it is suggested that if sera are analysed at the same time before 12 months storage in a －80 $^{\circ}C$ freezer, the storing time does not affect the biochemical evaluation of the sera in SD rats.

• Bulletin of the Korean Chemical Society
• /
• v.33 no.7
• /
• pp.2156-2162
• /
• 2012

Many literatures focus on the biological relevance and the identification of biomarkers for disease activity assessment while less attention has been paid to the development of standard procedures for sample preparation and storage based on liquid chromatography technique. The influencing factors including protein precipitation, storage temperature, storage time, and reconstitution by ultra pure water were analyzed employing HPLC-DAD. The effects were investigated from five participants over three months by principal components analysis (PCA) and the values of percent changes (PC). The samples with protein precipitation might slow the rate of bacterial enzymatic conversion. After protein precipitation, the average PC of urine samples ($0.136{\pm}0.013$, n = 5) is relatively less than that of the serum samples ($0.173{\pm}0.026$, n = 5) for three months. Minimal effects on metabolic profiles of serum and urine (PC < 0.15) are reasonable for metabolomic studies after protein precipitation and storage at $-20^{\circ}C$ for two months.

• Korean Journal of Veterinary Research
• /
• v.5 no.1
• /
• pp.97-123
• /
• 1965

Observations were made on the blood picture of total 196 heads of healthy Korean cattles, including 98 males and females in the purpose of determination of blood chemical values and their sex differences and seasonal variations during one year period from December, 1963 to November, 1964. The blood sampling were scheduled by random in four different seasons and the sample size of both sex included in each season were designated to be same size. The ranges, averages or mean values of the blood glucose, total serum protein, serum globulin, serum albumin, total non-protein nitrogen, blood urea nitrogn, total serum cholesterol, serum inorganic phosphorus and serum calcium were determined in this studies and their respective standard deviation, standard error of means, sex differences and seasonal variations were as follows. 1. The blood glucose values for the male ranged from 32.8 to 70.0 mg/100cc. with a mean of $49.781{\pm}0.823mg/100cc$; for the female the range was 32.0 to 64.0mg/100cc. with a mean of $47.235{\pm}0.782mg/100cc$. Sex difference showed significant at 5% level and seasonal variation was highly significant at 1% level. 2. The total serum protein values for the male ranged from 5.61 to 8.83 gm/100cc with a. mean of $7.366{\pm}0.062gm/100cc$; for the female ranged from 5.53 to 8. 43 gm/100cc. with a mean of $6.832{\pm}0.063gm/100cc$. Sex difference and seasonal variation was not significant. 3. The serum globulin values for the male ranged from 2.97 to 4.78 gm/100cc. with a mean of $3.961{\pm}0.039gm/100cc$.; for the female ranged from 2.87 to 4.41 gm/100cc. with a mean of $3.699{\pm}0.037gm/100cc$. Sex difference showed highly significant at 1% level and seasonal variation was not significant. 4. The serum albumin values for the male ranged from 2.58 to 4.21 gm/100cc. with a mean of $3.405{\pm}0.029gm/100cc$.; for the female ranged from 2.39 to 4.10 gm/100cc. with a mean of $3.204{\pm}0.031gm/100cc$. Sex difference showed highly significant at 1% level and seasonal variation was not significant. 5. The total non-protein nitrogan values for the male ranged from 19.1 to 44.8 gm/100cc. with a mean of $31.166{\pm}0.582mg/100cc$.; for the female the range was 15.2 to 50.5 mg/100cc. with a mean of $28.89.6{\pm}0.673mg/100cc$. Sex difference showed significant at 5% level and seasonal variation was highly significant at 1 % level. 6. The blood urea nitrogen values for the male ranged from 6.4 to 28.3 mg/100cc. with a mean of $13.371{\pm}0.466mg/100cc$.; for the female the range, was 6.0 to 26.9 mg/100cc. with a mean of $13.631{\pm}0.321mg/100cc$. Sex difference was not significant and seasonal variation showed highly significant at 1 % level. 7. The total serum cholesterol values for the male ranged from 60.0 to 238.6 mg/100cc. with a mean of $140.897{\pm}2.826mg/100cc$.; for the female ranged from 50.0 to 243.0 mg/100cc. with a mean of $124.840{\pm}3.553mg/100cc$. Sex difference and seasonal variation showed highly significant at 1% level. 8. The serum inorganic phosphorus values for the male ranged from 3.5 to 7.8 mg/100cc. with a mean of $5.426{\pm}0.096mg/100cc$.; for the female ranged from 3.1 to 8.8 mg/100cc. with a mean of $5.570{\pm}0.128mg/100cc$. Sex difference and seasonal variation showed no significant. 9. The serum calcium values for the male ranged from 7.8 to 12.8 mg/100cc. with a mean of $10.761{\pm}0.102mg/100cc$.; for the female ranged from 8.0 to 13.0 mg/100cc. with a mean of 10. $756{\pm}0.097mg/100cc$. Sex difference was not significant and seasonal variation showed highly significant at 1% level. 10. The age of test group ranged from 2 years to 6 years in both sex and the averageage were, $4.45{\pm}0.114$ years in male and $4.50{\pm}0116$ years in female. Sex difference and seasonal variation of age were not found to be significant.

• Bulletin of the Korean Chemical Society
• /
• v.34 no.6
• /
• pp.1698-1702
• /
• 2013

Glucose is a common medical analyte measuring in human serum or blood samples. The development of a primary method is necessary for the establishment of traceability in measurements. We have developed an isotope dilution liquid chromatography tandem mass spectrometry as a primary method for the measurement of glucose in human serum. Glucose and glucose-$^{13}C_6$ in sample were ionized in ESI negative mode and monitored at mass transfers of m/z 179/89 and 185/92 in MRM, respectively. Glucose was separated on $NH_2P$-50 2D column, and the mobile phase was 20 mM $NH_4OAc$ in 30% acetonitrile/70% water. Verification of this method was performed by the comparison with NIST SRMs. Our results agreed well with the SRM values. We have developed two levels of glucose serum certified reference material using this method and distributed them to the clinical laboratories in Korea as samples for proficiency testings. The expended uncertainty was about 1.2% on 95% confidence level. In proficiency testings, the results obtained from the clinical laboratories showed about 3.6% and 3.9% RSD to the certified values. Primary method can provide the traceability to the field laboratories through proficiency testings or certified reference materials.

• Asian-Australasian Journal of Animal Sciences
• /
• v.19 no.9
• /
• pp.1298-1304
• /
• 2006

The aim of this study was to provide basic haematological information to allow improved nutritional management for velvet production in spotted deer (Cervus nippon) by investigating biochemical changes in blood values during the antler growth period. Blood samples, obtained from the jugular vein of twenty-five deer, were taken every 10 days from casting (day 0) to harvesting (day 50) of velvet antler. Negligible changes were found in the concentrations of total protein, albumin, and creatinine during the antler growth period, but there were significant changes in the concentrations of urea (p<0.05) and uric acid (p<0.01). The concentration of triglyceride was significantly higher (p<0.05) during the antler growth period compared to casting time, while serum high-density lipoprotein concentrations were low and remained unchanged during the antler growth period. Serum glucose concentration increased (p<0.05) significantly and was slightly changeable during antler growth. The serum concentrations of Ca and P did not fluctuate during antler growth, while those of Na, K and Cl showed slight differences between the time of casting and the rest of the antler growth period. No significant changes in concentrations of AST, ALT, amylase, CK, GGT and LDH were detected during the antler growth period. However, the concentration of ALK-P increased during antler growth reaching its peak on day 50 after casting. We found a significant difference in the concentration of ALK-P between the time of casting and the rest of the antler growth period (p<0.01). Consequently, antler growth was associated with mild changes in measured serum biochemical values with the exception of ALK-P activity in spotted deer.

• Korean Journal of Animal Reproduction
• /
• v.24 no.2
• /
• pp.163-170
• /
• 2000

This study was conducted to examine the effects of recombinant Bovine Somatotropin (rBST), vitamin E(Vit. E) and selenium (Se) administration on the blood chemical values and hormone concentrations of serum in Hanwoo sires. Hanwoo sires were randomly assigned to five groups; 1) control, 2) rBST,0.09mg/kg body weight (BW), 3) Vit E, 1,500IU/kg BW, 4) Se 0.1mg/kg BW, 5) Vit E, 1,500IU plus Se 0.1mg/kg BW. rBST, Vit. E and Se for each experimental group were given 6 times at 15 days interval by intermuscular injection. Blood samples were collected ten times for experimental periods and separated the serum by centrifugation and stored into deep freezer. The concentration of albumin was the highest in Se group than those of any other groups (P＜0.05) and Vit. E plus Se group was significantly higher than in the control and rBST groups (P＜0.05). The concentrations of blood urea nitrogen (BUN) and creatinine were significantly higher in rBST group than any other groups (P＜0.05). The concentration of total protein in rBST, Se and Vit. E plus Se groups were significantly higher than in control group (P＜0.05). The concentrations of calcium, cholesterol,, glucose, inorganic phosphorus and triglycerides in serum were not difference in all experimental groups (P＞0.05). The concentration of estradiol was slightly higher in Se and Vit. E plus Se groups than those of any other groups, but not significantly difference among the experimental groups (P＜0.05). The concentration of testosterone was not affected by the administration of rBST, Vit. E and Se.

• Journal of Nutrition and Health
• /
• v.36 no.3
• /
• pp.262-269
• /
• 2003

The effect of dietary supplementation of the two oriental medicinal prescriptions, Sypjeondaebotang or Jahyulyanggeuntang, on iron bioavailability was evaluated in rats which were depleted of iron by being fed an irondeficient diet for 4 weeks. Seventy two iron-depleted rats were randomly divided into 3 groups (n = 24) , and fld one of the following experimental diets for 4 (n = 8), 12 (n = 8), and 25 days (n = 8) : Control diet (CD), Sypjeondaebotang-supplemented diet (SD), Jahyulyanggeuntang -supplemented diet (JD). The CD contained 20 mg Fe/kg diet as FeSO4, and the SD or JD was identical except that the respective oriental medicinal prescription extract was included at the level of 4 g/kg diet. Animals fed the SD and JD for 25 days consumed significantly less food (p＜0.001), but showed no change in food efficiency ratio compared to those that were fed the CD. Serum iron concentration and transferrin saturation values were significantly higher in rats fed the SD for 25 days compared to those that were fed the CD for the same period (p < 0.05). The values of serum UIBC (p < 0.05) and TIBC (p > 0.05) were lower in rats fed the SD compared to those fed the CD. Dietary supplementation of Sypjeondaebotang during the period of iron repletion significantly increased blood levels of hemoglobin (p < 0.05) and hematocrit (p < 0.01) measured at day 12, and increased mean corpuscular volume (p < 0.05) measured at day 25, compared to the values for the CD rats. Regression analyses of hemoglobin-repletion bioassay data from rats fed the SD and JD showed the relative biological values of 123% and 99%, respectively, calculated against the slope for the CD rats. Apparent iron absorption and retention values were also significantly lower (p < 0.05) in rats fed the SD for 25 days than those for the CD rats. Based on the results from diverse biochemical indices of iron status and the chemical balance study, the effect of Jahyulyanggeuntang on iron bioavailability appears to be less prominent than that for Sypjeondaebotang. Taken together, these results indicate that Sypjeondaebotang has a positive effect in restoring iron depletion by increasing the iron bioavailability in rats.