• Korean Journal of Animal Reproduction
• /
• v.14 no.2
• /
• pp.107-114
• /
• 1990

These experiments were undertaken as a basic study to develop immunocontraceptive vaccine and to understand the role of zona pellucidae in early fertilization process by identifying the monoclonal and polyclonal antibody to porcine zona pellucidae and polyclonal antibody to mouse zona pellucidae by indirect ELISA and indirect immunofluorescence test. The results obtained in these experiments were summarized as follows : 1. The titer of the antibodies to zona was determined by indirect ELISA using solubilized porcine zona coated plates. Both monoclonal and rabbit polyclonal antibodies showed very high titers ; O.D at 1 : 12,800 dilution of antibodies was still significantly higher than that of non-immunized control serum. Rabbit anti-mouse zona pellucidae sera also reacted with porcine zona pellucidae. 2. By indirect immunofluorescence test strong fluorescences were observed on the egg treated with homologous and heterologous rabbit polyclonal antibodies and FITC lablled 2nd antibodies and found to crossreact strongly with the eggs from the pig and mouse. While weaken fluorescences were observed on the eggs treated with monoclonal antibodies.

• Journal of fish pathology
• /
• v.22 no.3
• /
• pp.219-228
• /
• 2009

The genomic and subgenomic RNAs of fish nodavirus encode the four proteins, protein A, capsid protein, non-structural protein B1 and B2. In this study, we describe the immune response of olive flounder Paralichthys olivaceus immunized with live fish nodavirus or recombinant capsid protein, non-structural protein B1 and B2 expressed in E. coli. Nodavirus-infected flounder produced antibodies to capsid protein, B1 and B2 and nodavirus-neutralizing activities were detected in the serum of the nodavirus-infected flounder. The flounder were immunized against the three recombinant proteins of fish nodavirus and the sera from these immunized fishes were assayed for nodavirus-specific antibody by ELISA and a neutralization test. In the immunized flounder, all three recombinant proteins induced the production of similar levels of antibody, but only the antibody to capsid protein significantly neutralized nodavirus. These results indicate that all three nodaviral proteins are immunogenic in flounder, but only the capsid protein can induce neutralizing antibody against nodavirus.

• Parasites, Hosts and Diseases
• /
• v.46 no.4
• /
• pp.279-283
• /
• 2008

To examine humoral immune responses in the host, we measured serum antibody levels in different strains of mice (ICR, BALB/c, and C3H) experimentally infected with Neodiplostomum seoulense. Specific IgG antibody levels were increased remarkably with little difference among 3 strains of mice infected with N. seoulense from day 7 to 35 post-infection. More target proteins of adult parasites reacted with IgG at the time when the worm recovery decreased compared with other times. More than 20 protein bands, from 14 kDa to 94 kDa in size, were separated from the crude antigen of N. seoulense adults by SDS-PAGE, and among them 26, 30, 35, 43, 54, 67, and 94 kDa proteins were the major antigenic proteins. The results suggest that significant IgG antibody responses occur against N. seoulense in mice and this may be related with expulsion of worms.

• The Korea Journal of Herbology
• /
• v.23 no.1
• /
• pp.23-28
• /
• 2008

Objectives : Socheongryong-Tang(小靑龍湯, SCRT), a herbal remedy, has been widely used to treat respiratory disease such as cough and asthma in Oriental countries. Recent years SCRT was known as anti-allergic agent. However, its therapeutic mechanisms including immunoglobuline such as IgE, IgG1, IgG2a productions are unclear. Methods : We investigated the effects of SCRT on levels of antigen specific total antibody, IgE, IgG1, IgG2a using ELISA method in serum from allergen-induced asthma mice. Results : SCRT decreased level of antigen specific IgE significantly. And SCRT treated mice showed downward tendency of IgG1, a Th1 relative antibody, level. But, SCRT did not affect levels of antigen specific total antibody and IgG2a, a Th1 relative antibody. Conclusions : we demonstrated the strong possibility of SCRT as a complementary or alternative drug to western drug also demonstrated that regulation of Th1/Th2 imbalance may be one of mechanism contributed to treatment for respiratory disease by SCRT.

• Applied Microscopy
• /
• v.28 no.4
• /
• pp.527-538
• /
• 1998

This study was performed to investigate the activity of antibodies in the tissues of Paragonimus westermani at the different developmental stages. Enzyme-linked immunosorbent assay (ELISA) and Immunelectron microscopy (IEM) were applied, using the dog sera infected with metacercariae isolated from Cambaroides similis. These dog sera were obtained from 3rd to 96th week after infection by bleeding. The supernatants of homogenated worms for worm antigen were used. The worm tissues were embedded in Lowicryl HM 20 medium, treated with infected serum and protein A gold complex (particle size; 12 nm) and observed by electron microscope. In the pattern of antibody levels by ELISA test in all developemental worm antigens, the activity of antibody was very weak in the 3rd week, but strong in the worm antibody from 4th to 20th week after infection. Its activity was maintained even till 96th week. The antibody level of the L2th week worm antigen was higher than those of the 20th and 48th week worm antigens. Generally, many gold particles were observed on the secretory granules and the epithelial lamellae. Thus, it was concluded that the antigenic materials in the developmental worm tissues were especially concentrated on the secretory granules in the parenchymal tissues and the epithelial lamellae in the lumen of the caecum.

• The Journal of Korean Obstetrics and Gynecology
• /
• v.36 no.1
• /
• pp.1-22
• /
• 2023

Objectives: This study was performed to analyze randomized controlled trial, which studied the effect of herbal medicine treatment on Antiphospholipid antibody positive recurrent abortion. Methods: We searched for randomized controlled trial of last 20 years based on Antiphospholipid antibody positive recurrent abortion and herbal medicine. The paper search was conducted through 7 online databases on July 16, 2022. Results: 9 studies were selected after selection and exclusion criteria. 5 studies compared combined treatment of herbal and western medicine, with western medicine alone. 4 studies compared herbal medicine alone with western medicine. Comparing with control group, the treatment group showed much improvement on conversion rate of anti-phospholipid antibodies, serum hCG and progesterone levels, pregnancy duration or fertility rates, and various symptoms. Conclusions: In this study, we found out benefit of herbal medicine with Antiphospholipid antibody positive recurrent abortion. For reliable evidence, further research is needed to establish safety of herbal medicines, standardize symptom criteria and specify the treatment course.

• Journal of agricultural medicine and community health
• /
• v.12 no.1
• /
• pp.117-123
• /
• 1987

Detection of IgG antibody in clonorchiasis has been accomplished through various serodiagnostic procedure including complement fixation test, gel diffusion test, indirect fluorescent antibody test, indirect hemagglutination test etc. In this report enzyme immunoassay (ELISA) and indirect hemagglutination test (IHA) were used to determine IgG serum antibody levels before and after therapy with praziquantel. Briefly, sera from 62 cases of confirmed human clonorchiasis were examined before and after treatment with praziquantel. Among 62 cases treated 25 cases were categorized as completely cured groups by formalin-ether and careful examination of 4 cellophane thick smered slides at 18 months after treatment. The sera of 25 cases of cured groups were examined again by ELISA and IHA, and com-pared to the previous data. The results obtained were as follows; 1) Sensitivity of IHA test was 83.6% when cut-off titer of 1:8 was applied. No sera obtained from 10 normal healthy control showed positive reaction. 2) Twenty cases (80.0%) out of 25 cured one showed negative results by IHA at 18 months after treatment. 3) Although 5 cases showed positive titer even 18 months after treatment 3 cases of them showed decreased antibody titer. However 2 cases did not show any response. 4) Even though almost all cases showed de- creased ELISA value, only 11 cases (44.0%) out of 25 patients showed negative results by ELISA at 18 months after treatment. In conclusion, it is suggested that, while IgG ELISA for detecting long persisting antibody was more sensitive than IHA, IHA results more conclusively indicated effective treatment in clonorchiasis by negative conversion than did the results of ELISA.

• Journal of the korean veterinary medical association
• /
• v.25 no.5
• /
• pp.282-291
• /
• 1989

Incidence of infectious bronchitis virus(IBV) infection in vaccinated breeder chickens was investigated by hemagglutination inhibition(HI)test for IBV using Mass 41 antigen. In the breeder chickens with the reduced egg production. chalky deposit. wrinkled

• Proceedings of the PSK Conference
• /
• 2001.04a
• /
• pp.198.1-198.1
• /
• 2001

• Korean Journal of Veterinary Research
• /
• v.26 no.2
• /
• pp.265-276
• /
• 1986

The bovine serum factor influencing the growth of swine testicle (ST) cell and the END effect of hog cholera SN test was studied. Throughout the experimental studies. following results were obtained and summarized. 1. Bovine whole serum of 16(76.2%) and 4(19.0%) samples out of 21 have shown a positive ST cell growth and the END effect, respectively. However, all of 21(100%) and 8(38.1%) samples out of 21 serum supernatant fractions, prepared from the bovine whole serum, have shown positive ST cell growth and END effect, respectively. 2. In the SDS-polyacrylamide gel electrophoretic analysis of the bovine whole serum and the supernatant fractions, ST cell growth inhibiting factor was proved present in globulin fraction and in whole gel plate as a diffusible component. 3. The END ineffective component present in the whole serum and its supernatant fraction was proved to be BVDV neutralizing antibody. 4. The difference of osmolarity, optical density, pH, degree of precipitant formation following heat cold treatment, A/G ratio as we11 as electrophoretic pattern and NDV SN index of the samples were not correlated to the degree of 57 cell growth and to the END effectiveness.