• Korean Journal of Veterinary Research
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• v.38 no.4
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• pp.793-802
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• 1998

Salmonellosis caused by a number of serotypes of Salmonella is an infectious, acute or chronic, zoonotic disease and characterized by enteritis and diarrhea, septicemia in animal. In these studies we investigated the prevalent serotypes of Salmonella causing animal salmonellosis in Korea and the 71 strains of Salmonella spp. were isolated from materials such as mesenteric lymph nodes, fecal samples from slaughtered animal. With the identification test results, the most prevalent serotypes were, in order, S stanley 31 strains (43.7%), S typhimurium 19 strains (26.8%) and S montevideo 11 strains (15.5%), respectively. And we could establish the method for detection of antibodies to broad variety of Salmonella serotypes. Lipopolysaccharide(LPS) antigen extracted from Salmonella was more sensitive and specific than outer membrane protein antigen from that for detection of Salmonella antibody by using an indirect ELISA. The optimal concentration of antigen was 100ng/ml of LPS, the dilutions of conjugate and serum were 1 : 1,000~2,000 and 1 : 200~400, respectively. The mix LPS-ELISA which was used by mixing LPS from S typhimurium (group B), S choleraesuis (group C) and S enteritidis (group D) were more rapid and effective than that used LPS from individual strain for detection of Salmonella serogroup O4, O7 and O9 antibody at the same time. We could obtain the high values of optical density ($0.73{\pm}0.32$) by mix LPS-ELISA on the farm which had occurred salmonellosis, but very low values of $0.17{\pm}0.06$ on the negative farm of salmonellosis. So, the mix LPS-ELISA may be used to monitor the serological surveillance for the presence of infection with a number of serotypes of Salmonella and would be useful for prevention and control of salmonellosis in animal.

• Pediatric Infection and Vaccine
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• v.13 no.2
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• pp.99-105
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• 2006

Purpose : The serotyping results of the Quellung reaction was compared with the newly developed multiplex latex assay and the sensitivity of the Quellung reaction was compared. Methods : We checked the serotypes of 35 samples obtained from patients treated at Yonsei University Medical Center using the multiplex latex bead method and compared the results with the serotypes previously obtained via the Quellung reaction. Results : A decrease in the mean fluorescence was detected in the samples tested with the multiplex assay. Seventeen samples out of the 27 samples agreed to the results of the Quellung assay. We were only able to confirm the concordance of 11 serotypes out of 14 serotypes available. Conclusion : The Quellung reaction is time consuming procedure and prone to errors even with expertise in the procedure, and other alternate methods in serotyping have been investigated to overcome these problems. The newly developed multiplex latex bead assay can test more samples at the same time and has a higher degree of sensitivity. A large scale trial is required to test the sensitivity of the new assay across various serotypes along with efforts to increase the sensitivity of the Quellung assay. The preliminary data suggests that this method may be widely used.

• Korean Journal of Veterinary Service
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• v.31 no.1
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• pp.1-16
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• 2008

Salmonella infections cause the disease in pigs but also some zoonotic Salmonella serotypes can be transmitted to human through swine products, resulting in food poisoning. The objective of this study was to investigate the bacteriological prevalence and detection of invA gene using Salmonella specific polymerase chain reaction (PCR), the epidemiological characteristics related to Salmonella strains cultured from pig samples in Gangwon areas using serotyping, random amplified polymorphic DNA (RAPD) and pulsed field gel electrophoresis (PFGE) methods. During the period of November 2001 through April 2002, 1,174 ileocecal lymph node were collected from the slaughtered pigs raised in 38 farms located in Gangwon province. The samples were submerged in boiling water and macerated in saline and lymph node homogenates were inoculated into Tetrathionate broth with iodine (TTB, Difco, 0.5% iodine was added) for enrichment growth. Then additional tests were performed using several mediums, and suspects were identified by API 20E kit (BioMerieux) and PCR. Of total 1,174 samples from 38 farms, 44 (3.7%) were isolated as Salmonella spp from 13 farms (34.2%). Of 44 isolates, 31 were in Yangyang region, followed by 9 in Goseong, 2 in both Gangneung and Sokcho. However, there was no difference in regional isolation frequency. All isolates have a 521bp amplified product in Salmonella specific PCR with primer invA which encodes in proteins for invasion of epithelial cells. Of 44 recovered serotypes, 23 (52.3%) were S Eingedi, 10 (22.7%) S Schwarzengrund, 9 (20.5%) S Typhimurium, and 2 (4.5%) S Mbandaka. In RAPD analysis, there appeared to be unique bands distinguishing each serotype, although similarities exist between the different serotypes. Four serotypes of 44 Salmonella isolates appeared to fall into 14 different RAPD types. In PFGE analysis, 9 S Typhimurium were tested with XbaI enzyme and SpeI enzyme. The combination of results obtained with two enzymes subdivided the 9 S Typhimurium into 4 PFGE types.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.99-99
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• 2003

• Korean Journal of Microbiology
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• v.23 no.3
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• pp.223-229
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• 1985

We have previously isolated seven strains of spiral form bacteria, which have shown several similar characteristics to Leptospira from patient with epidemic pulmonary hemorrhagic fever and matural paddy water. Further studies have performed to characterize them. All of the isolated bacteria were aerobic, gram negative and spiral form and their growth were completely inhibited at temperature below $13^{\circ}C\;and\;50^{\circ}C$. In sensitivity test to drugs, the bacteria were resistant to the bile salts, 5-Fu and Amphotericine B, but sensitive to the 8-Azaguanine. The isolated bacteria as well as 5 serotypes of Leptospira interrogans reacted the patient sera and it was also shown that antisera from rabbit immunized with the isolated bacteria reacted with 5 serotypes of Leptospira interrogans. These results suggest that the isolated bacteria may belong to the genus Leptospira.

• The Korean Journal of Food And Nutrition
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• v.11 no.4
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• pp.416-419
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• 1998

Identification of escherchia coli K1 polysaccharide antigen isolated from urine specimens of urinary tract infections in children were performed from of 1992 to 1993 in Kyoto, Japan. The serotypes of E. coli were categorized that O1:H7, O2:H6, O2:H7, O16:H6, O18:H7, O18:H￣, and O135:H44 among 14 strains isolated from urine specimens of urinary tract infections in children by the serological test. And, one strain (O18:H￣, isolation rate: 7.1%) of E. coli K1 polysaccharide antigen among 14 strains were isolated from urine specimens of urinary tract infections in children by the bacteriophage test.

• Korean Journal of Veterinary Service
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• v.17 no.3
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• pp.181-189
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• 1994

Sixty-three Salmonella Cultures were isolated from 600 msenteric lymphnodes and 600 rectal contents of apprently healthy pigs slaughtered at abattoris in Hapchun, changnyong, Keochang. The result observed were summaried as follows ; 1. Fifty-nine out of sixty-three cultures were serotyped and Salmonella enteritis(20 cultures) 2. Was found to be the most predominant serotpye. The remainder comprised of 15 serotypes were ; S. typhimuriu, S. derby, S. wagenia, S. infantis, S. thompson, S. tennessee, S. mission, S. anatum, S. give, S. nuwlands, S. newingtion, S. newhaw, S. neumuenstes, S. senftenberg, S. gbana 3. The antibiotic susceptibility of 9 were ; Baytril, Streptomycin, Cephalothin, Ampicillin, Tetracycline, Sulfadimethoxine, Rifampicin, Gentamicin, Kanamycln.

• The Journal of the Korean Society for Microbiology
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• v.21 no.1
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• pp.127-131
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• 1986

The mating types and serotypes of 10 clinical isolates of Cryptococcus neoformans have been investigated. Seven isolates were serotype A and three were serotype D and thus they fell in C. neoformans var. neoformans. Mating types of six isolates were found $\alpha$ and two were $\alpha$ but another two isolates were untypable. Enzyme linked immuno-sorbent assay(ELISA) using rabbit hyper-immune serum to cryptococcal polysaccharides was well adapted to the analysis of capsular polysaccharides in sera of the patients with cryptococcal meningitis.

• Bulletin of the Korean Chemical Society
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• v.29 no.7
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• pp.1315-1319
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• 2008

A trisaccharide, 6d-Altro-Hepp$\alpha$ (1$\rightarrow$3) GlcNAc$\beta$ (1$\rightarrow$3) Gal$\alpha$ (1$\rightarrow$$OCH_2CH_2N_3$, as an O-antigenic repeating unit of Campylobacter jejuni serotypes O:23 and O:36, was synthesized. Coupling of the 6d-altro-Hepp$\alpha$ (1$\rightarrow$3) GlcNAc$\beta$ (1$\rightarrow$SEt donor with Gal$\alpha$ (1${\rightarrow}OCH_2CH_2Cl$ acceptor in the presence of NIS-TfOH promoter afforded the trisaccharide having the $\beta$ (1$\rightarrow$3) Gal linkage. $\beta$ -Stereospecificity and the desired regioselectivity for the 3-OH Gal are obtained. Subsequent hydrogenation, acetylation, azide displacement, hydrazinolysis, Nacetylation, and finally deacetylation furnished the title trisaccharide hapten for further glycoconjugation.

• Korean Journal of Veterinary Research
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• v.60 no.4
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• pp.225-228
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• 2020

Apx toxins are a virulent factor of Actinobacillus pleuropneumoniae (App). At least four genes, apxC, apxA, apxB, and apxD, are involved in the release of Apx toxins from App. apxA encodes Apx toxins, whereas apxB and apxD encode exporters. Some serotypes of App such as serotype 2 retain apxIBD, apxIICA, and apxIIICABD. Although the specificity of the ApxIB/ApxID exporter to ApxII has been established in those serotypes, that to ApxIII is under-studied. We constructed an apxIB- and apxID-lacking mutant strain of the App serotype 2 to study whether the ApxIB/ApxID exporter is capable of secreting both ApxII and ApxIII toxins.