• Title/Summary/Keyword: serological test

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The Occurrance of Velogenic Viscerotropic Newcastle Disease Virus in an Adult Peacock (성숙 공작(Pavo cristatus)에서 발생한 내장 친화형 뉴캣슬병 바이러스 강독주)

  • 조경오;박남용;강문일;고홍범;이근우
    • Journal of Veterinary Clinics
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    • v.18 no.2
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    • pp.152-155
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    • 2001
  • A two-year-old male peacock (Pavo cristatus) showed acute watery green diarrhea, followed by neurological signs including torticollis and muscular tremor. By the hemagglutination inhibition test for detecting the antibody against the Newcastle disease virus (NDV), the peacock serum inhibited the agglutination of chicken red blood cells. Grossly distinctive hemorrhagic lesions were found in the mucosa of proventiculus and intestine and lung. The spleen revealed multiple variable sized necrotic foci. Histologically, the mucosa of gastrointestinal track had hemorrhagic lesions and some of them underwent ulceration. The spleen exhibited multiple variable sized necrotic foci in which fibrin exudation was marked. Central nervous system had mild non-suppulative menin-goencephalitis consisting of vasculitis, perivascular hemorrhage, gliosis and meningitis. The cells particularly in the cerebellum were degenerative to necrotic. Some of these nerve cells revealed characteristic peripheral chromatolysis. From the present serological and pathological findings, it is suggested that NDV causing death of peacock was velogenic viscerotropic strain. This is the first report of the occurrence of velogenic viscerotropic NDV in an adult peacock in Korea.

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Isolation and Biochemical Properties of Pasteurella multocida from the pneumonic lungs of swine in Cheju (제주지방 돼지의 폐병변으로부터 Pasteurella multocida 분리 및 생화학적 특성)

  • 김옥녀;이두식;문호규;김우택;서문현;배종희;임윤규;조길재
    • Korean Journal of Veterinary Service
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    • v.18 no.2
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    • pp.124-132
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    • 1995
  • The present study was conducted to investigate the incidence of Pasteurella multocida infection in cheju swine from March 1994 to December 1994 isolated organisms were identified by the biochemical properties, cellulose serological type and antibiotic susceptibilities. Pasteurella multocida was isolated from the Lungs of 96 pigs with pneumonia(51 %) among 188 slaughtered pigs. The majority of p multocida isolates were identical to those of the standard strains. On the classification of the capsular type of the isolated p multocida it consist of the 88 isolates of type A(91.6%) 2 isolates of type D (2%) and un classified 6 types(6.2%). The majority of the 96 isolates of p multocida highly susceptible to the antibiotics including ampicillin(Am), cephalotin(Ce), erythromycin(Em), gentamycin(Gm), kanamycin(Km), lincomycin(Lm), neomycin(Nm), penicillin(Pc), streptomycin(Sm), solfametoxazol/trimethoprim(Sxt) and tetracycline(Te)

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A case of Idiopathic Bilateral Brachial Neuritis Involving the Bilateral Phrenic Nerves (양측 횡격막신경을 침범한 원인불명의 양측 상완신경염 1예)

  • Kwak, Jae-Hyuk;Lee, Dong-Kuck;Kwon, Oh-Dae
    • Annals of Clinical Neurophysiology
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    • v.7 no.1
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    • pp.28-30
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    • 2005
  • Bilateral brachial neuritis is clinically uncommon and accidentally involvement of bilateral phrenic nerves is rarely reported. We experienced a 26 year old man who developed subacute onset of asymmetric bilateral shoulder and arm weakness. The weakness slowly aggravated and finally suffered from dyspnea due to bilateral phrenic nerve palsy. Cervical spine MRI and CSF study showed no abnormality. Viral markers and other serological test showed no specific finding. Electromyographic study showed bilateral brachial axonal polyneuropathy with cervical and upper thoracic polyradiculopathy. And bilateral phrenic nerve conduction study showed no resopnse. He showed no improvement for 10 months after treatment and managed with continuous artificial ventilation. We report a case of idiopathic bilateral brachial neuritis accidentally involving bilateral phrenic nerves.

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Recurred Sparganosis 1 Year after Surgical Removal of a Sparganum in a Korean Woman

  • Lee, Young-Il;Seo, Min;Park, Hyun-Woo
    • Parasites, Hosts and Diseases
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    • v.52 no.1
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    • pp.75-78
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    • 2014
  • Sparganosis, an infection due to the plerocercoid of Spirometra erinacei, are found worldwide but the majority of cases occur in East Asia including Korea. This report is on a recurred case of sparganosis in the subcutaneous tissue of the right lower leg 1 year after a surgical removal of a worm from a similar region. At admission, ultrasonography (USG) of the lesion strongly suggested sparganosis, and a worm was successfully removed which turned out to be a sparganum with scolex. Since sparganum has a variable life span, and may develop into a life-threatening severe case, a patient once diagnosed as sparganosis should be properly followed-up for a certain period of time. Although imaging modalities were useful for the diagnosis of sparganosis as seen in this case, serological test such as ELISA should also be accompanied so as to support the preoperative diagnosis.

Turnip Mosaic Virus Isolated from Rorippa islandica Borb (속속이풀(Rorippa islandica Borb.)에서 분리한 순무 모자이크 바이러스)

  • 최준근;최국선;최장경;유병주;정태성
    • Korean Journal Plant Pathology
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    • v.10 no.2
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    • pp.136-139
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    • 1994
  • Turnip mosaic virus (TuMV) was isolated from Rorippa islandica showing mild mosaic symptom in growing field of Chinese cabbage and radish. Identification of the virus was based on host range, transmission by aphids, electron micrograph, serological reaction and hybridization detection. The virus systemically infected on Chenopodium quinoa, Nicotiana clevelandii, N. glutinosa, Brassica rapa, B. campestris subsp. pekinensis and Raphanus sativus, whereas showed local infection on C. amaranticolor, Gomphrena globosa and Tetragonia tetragonoides. The virus was transmitted by aphid (Myzus persicae). The virus particle was filamentous with 720$\times$12 nm in length, and reacted positively with an antiserum of TuMV in agar gel double duffusion test. In slot-blot hybridization using the digoxigenin(DIG)-labeled RNA probe, TuMV-RNA could be detected in sap of R. islandica infected with the virus. This is the first report of a natural infection of that virus on R. islandica.

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Studies on the Streptococcus suis isolated from slaughtered pigs (도축돈에서 분리된 Strptococcus suis에 대한 연구)

  • 윤선종;고홍범
    • Korean Journal of Veterinary Service
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    • v.20 no.3
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    • pp.281-288
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    • 1997
  • Streptococcus suis has been identified as a major cause of contagious disease in pigs Ithas been isolated worldwide from pigs suffering from meningitis, bronchopneumonia, polyserositis, polyarthritis and septicemia. Two hundred and fifty-five lung samples of slaughter pigs with gross lung lesions were collected from Jan. to Dec. 1995 in southern Chonnam area. Isolation of S suis were tested by biochemical, serological methods and antimicrobial susceptibility. The results were summerrized as follows ; 1. S suis was Isolated from 30 of 254 pneumonic lungs, 14 Isolates were isolated only, 12 were isolated with p. multocida and 4 were isolated with unidentified Gram positive cocci. 2. In biochemical characteristics studies, all isolates were not grown in 6.5% NaCl medium, and most isolates utilized L-leucine-2-naphtylamide and trehalose, didn't utilize sorbitol, ribose and L-arabinose. 3. In slide agglutination test, 6(20%) Isolates were classified as serotype 2, 4(13.3%) isolates were as serotype l/2, 16, 2 and 2(6.6%) were as serotype 1, 4, 13. 4. S suis isolates showed marked susceptibility to amoxicillin, oxacillin, cephalothin and cepoferazone and high resistance to kanamycin, streptomycin, tetracycline and erythromycin.

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Survey on Antibodies Against Bovine Leukemia Virus in Chung Buk Province (충청북도 소 백혈병 항체 조사 연구)

  • 최해연;정운선;유기조;오홍세
    • Korean Journal of Veterinary Service
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    • v.15 no.1
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    • pp.51-57
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    • 1992
  • A serological survey was carried out for the detection of antibody of Bovine Leukemia Virus (BLV) in nothern parts of Chung Buk area. The results were summarized as followed. 1. The overall positive rate was revealed as high as 15% with 48 positive cases out of 319 heads examined. 2. According to age, cattle of 4 to 7 ages showed relatively higher positive rate of 15% than other ages. 3. Seasonal differences of positive rate were not recognized. 4. BLV antibody titer of scales of cattles that from 5 to 15 heads farm were the highest. 5. With the result of blood test that of BLV positive cattle, the number of WBC was slightly Increased, but other records were normal.

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Biological and serological characteristics of Pseudomonas aeruginosa from clinical specimens (임상검체로부터 분리한 녹농균의 생물학적 및 혈청학적 특성)

  • 임은경;김영희;김영부;오양효
    • Journal of Life Science
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    • v.10 no.2
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    • pp.157-163
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    • 2000
  • One hundred eight strains of Pseudomonas aeruginosa isolated from the patients (sputum, urine, burn skin, stool and blood) of Pusan National University hospital were tested for exonezyme production, antimicrobial susceptibility and serotyping. The results obtained were as follow: In exonezyme production test, 50 strains (46.30%) produced both protease and elastase. Thirty three strains (30.55%) did not produce any exoenzyme, 18 strains (16.67%) produced only protease and 7(6.48%) stains only produced elastase. As the result of antimicrobial susceptibility by the disc diffusion method, most strains were resistant to sulfamethoxazole (96.30%). But the resistant rate against gentamicin and ticarcillin were 47.23% and 46.30% respectively. The resistant rate to other antibiotics were less than 40%. All strains could be serologically typed. Most strains were identified as type Ⅲ: among them, 51 strains were belonged to serotype E. The correlation of serotype and exoenzyme production was not found.

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Seroprevalence of Antigens to Bovine Viral Diarrhea Virus in Korean Calves of the Shown Healthy, Digestive and Respiratory Symptom (한우송아지에서 ELISA를 이용한 소 바이러스성 설사병 바이러스 항원 검출)

  • Chon, Seung-Ki;Park, Jin-Ho;Kim, Nam-Soo
    • Journal of Veterinary Clinics
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    • v.24 no.2
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    • pp.150-153
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    • 2007
  • The aim of this study was to investigate the prevalence of bovine viral diarrhea virus (BVDV) infection in Chonbuk province. Blood samples were taken from 92 korean calves to determined their serological status against BVDV, Capture enzyme-linked immunosorbent assay (ELISA) was used to test for antigen. The number of seropositive calves ranged from 3.3% to 12.9%. Antigens against BVDV were detected in 3.3% of healthy calves, 6.4% of digestive symptom calves, 12.9% of respiratory symptom calves, respectively. Sex and age of calves had no significant differences on the prevalence of BVDV. The results indicate that transmission of BVDV may have become exposed as a result of contact with acute infected or persistently infected cattle.

Prevalence of Leptospiral Antibodies in Sows (모돈에서의 Leptospira속균에 대한 혈중항체조사)

  • 강신석;곽학구;김홍기
    • Korean Journal of Veterinary Service
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    • v.16 no.1
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    • pp.65-69
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    • 1993
  • A serological survey was conducted to detect the type of Leptospirosis in sows. Antigen(live antigen) tested were L.icterohemorrhagiae, L.pomonal, L.canicola, L.Hardjo, L.australis, L.autumnalis, L.grippotyphosa, L.pyrogens, L.bataviae, L.hebdomadis. The survey was performed from J one 1992 to December 1992 by microscopic-agglutination test. The results were as follows 1, Among the serum samples of 92 heads of the sows, 6 heads of the sow(6.5%) were positive. 2. Among the positive samples of 6 heads, L.icterihemorrhagiae were 4 heads(4.3%) and L. pomona 2 heads (2.2%), respectively. 3. Antibody titers of positive sera were ranging from 1:200 to 1:1600. 4. The seroprevalence of leptospira in Chechon city, Chechon county, Danyang county that Chechon county(3.3%) was higher than that of other districts(1% -2.2%).

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