• Journal of Microbiology
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• 제34권2호
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• pp.124-130
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• 1996

A survey was conducted to determine the prevalance of infectious pancreatic necrosis virus (IPNV) on fish farms in Korea and the epidemiology of IPNV infection in the farmed rainbow trout. In total, 43 pools of rainbow trout with apparent signs of viral infection from five provinces were obtained and analyzed. Evident cytopathic effects, including karyopycnosis and cell destruction, were observed in CHSE (chinook samlmon embyro)-214 cells infected with the virus isolates. Of these, ten viral isolates were assumed to be IPNV based on biophysical properties. RNA analysis revealed that the isolates contained two-segmented RNA genomes, further indicating that the viral isolates are IPNV. Antigenic comparison of the IPNV isolates identified three distinct serological groups separable by the cross-neutralization test. Of the ten IPNV isolates, six could be classified as strain DRT, two as strain Ab, and two as strain VR299. We were not able to isolate new strain of IPNV or any isolate serologically similar to the standard strain Sp.poraceae and families of the Agaricales, they are genetically more related to the Polyporaceae. These results are consistent with morphological characters observed in those mushrooms. However, it is premature to conclude taxonomic status Ganoderma species in the present study employing small sample size.

• Parasites, Hosts and Diseases
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• 제21권2호
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• pp.265-269
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• 1983

간흡충증 환자 혈청에서 Thin layer immunoassay에 의하여 항체를 정량적으로 측정하여 감수성과 특이성을 알아보고, 효소표식면역 법(ELISA)에 의한 성적과 비교하였다. 냉동 건조한 간흡충 성충의 생리식염수 추출물을 항원으로 사용하였고, TIA는 Elwing등(1976)의 방법으로, 효소표식면 역법은 Veiler등(1974)의 방법에 의해 실시하였다. 1. 간홉충중 환자 60명의 혈청에서 TIA zone 크기의 평균은 4.14mm였고, 3mm 이상을 양성으로 판정하였을 때 감수성은 100%, 특이성은 61.1%였다. 2. 효소표식면역 법에 의하면 ELISA치 0.8이상을 양성으로 판정하였을 때 감수성은 88.3%, 특이성은 87.3%였다. 3. 혈청내 IgG 농도는 TIA zone 크기와 상관이 없었으나 좌소표식면역법에 의한 ELISA치와는 상관이 있어 그 계수는 0.69였다. 4. TIA zone 크기는 효소표식면역 법에 의한 ELISA치와 그 분포에 있어 상관이 없었다.

• 대한수의학회지
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• 제58권2호
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• pp.73-79
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• 2018

Outbreaks of porcine reproductive and respiratory syndrome virus (PRRSV) in vaccinated sow herds from occurrence to stabilization were monitored and analyzed in terms of serology and reproductive performance. Three different conventional pig farms experienced severe reproductive failures with the introduction of a type 1 PRRSV. These farms had adopted mass vaccination of sows using a type 2 PRRSV modified live vaccine (MLV). Therefore, to control the type 1 PRRSV, an alternative vaccination program utilizing both type 1 and type 2 MLV was undertaken. Following whole herd vaccinations with both types of MLV, successful stabilization of PRRS outbreaks was identified based on serological data (no viremia and downward trends in ELISA antibody titers in both sows and suckling piglets) and recovery of reproductive performance. Additionally, through comparison of the reproductive parameters between outbreak and non-outbreak periods, it was identified that PRRSV significantly affected the farrowing rate and the number of suckling piglets per litter at all three pig farms. Comparison of reproductive parameters between periods when the different vaccination strategies were applied revealed that the number of piglets born in total and born dead per litter were significantly increased after the introduction of the type 1 PRRS MLV.

• 한국동물학회지
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• 제31권4호
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• pp.251-264
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• 1988

동면 상태에서 혈액성분의 생화학적 변화치를 조사하고자 전남 각지에 서식 또는 광주직할시 근교에서 양식하고 있는 뱀장어, 개구리, 살모사 그리고 다람쥐를 활동기(7월∼8월), 잠입기(10월∼11월), 동진기(12월∼1월) 및 각려기(3월∼4월)에 채집하였다. 이들 동물들의 혈청에서 Total protein, Albumin, Globulin, A/G ratio, Glucose, Calcium, Urea함유량을 조사하여 얻은 성적을 비교 검토하여 다음과 같은 결론을 얻었다. 1 . Total protein의 변화는 각 동물사이에서 상당한 차이를 보이되 잠입기에 다소 증가하였다. 특히 다람쥐에서 Total protein 함유량이 다른 동물들 보다 많았다. Albumin함유량은 잠입기에 상승하였고 동면기에는 약간의 감소를 보이다가 각성기에는 현저한 감소를 보였다. gloulin함유량은 잠입기에 상당히 상승하고 동면기에 약간의 감소를 나타내었으며 각성기 인 봄철에는 많은 증가 현상을 보였다. 2. 혈청 Urea의 함유량은 동면의 각과정에 따라 상당한 차이가 있었다. 즉 잠입기에 약간 상승하고 동면기에는 저하를 보였으며 각성기에는 전 실험동물에서 검출되지 않았다. 3. 혈당의 변화는 잠입기에 대체로 저하하고 동면기에는 심한 저혈당 수준에 이르렀다. 각성기인 봄철에는 다람쥐를 제외하고는 동면기의 혈희량과 유사하였다. 4. 혈청 Calcium함유량의 변화는 활동기에 비하여 잠입기에는 상당한 증가현상을 보였으나동면기에는 전 실험동물이 거의 같은 수준으로 감소를 보이며 각성기에 까지 감소하였다. In order to the biochemical change of the serological components of hibernating animals, eels(hnguillo joponica Temminck et Schlegel), frogs (Rana nigromacurata Hallowell), snakes (Agkistro-don holds breuicaudus Stejneserl, squirrels (Tamias striatus Linnaeus) which were collected around Gwangiu city and the Chunnam area were observed in accordance with the phases of hibernation. The author described the hibernating cycle as active (June-July), prehibematins(October-Novemberl, hibernating (December-January) and posthibemating phase (March-Aprill. The values of total protein, albumin, globulin, hJG ratlo, calcium, glucose and urea were chiefly investigated in their serum. The results are as following: Total protein was shown to be consider-able in eels, frogs, snakes, squirrels and increased in prehibemation. Especially, the mean value was high in sqirrels. Albumin values was increased during prehibemation. But a little decrease was shown during hibernating and an outstanding decrease during posthibemation. The globulin value increased considerably during prehlbemation but decreased a little during hibernation phase. Changes of urea were shown to be considerable according to the stage of each hibem3-tion. A little increase was shown during prehibemation and decrease during hibernating stage. but urea was not found in posthibemation in any experimental animals. The glucose value was generally decreased during prehibematlon. An extremely little increase in glucose was shown during hibernation (hypoglycemia). All hibernating values except those for squirrels in spring posthibemating phase were at almost the same level. The calcium value was shown to be considerable during prehibemation in comparison with active phase. The same level of decrease was represented in all kinds of animals and continued at the stage of posthibemation.

• Journal of Nutrition and Health
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• 제38권3호
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• pp.197-202
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• 2005

Resistin, adiponectin, and leptin are hormones secreted by adipose tissue and are known to play an important role in adipose tissue metabolism. The purpose of this study was to evaluate the levels of adipocyte-derived hormones (resistin, adiponectin, leptin) in obese children aged 10-12 years. The subjects were 102 obese children with obesity index (OI) over $120\%$ and 51 control children with obesity index less than $120\%$ were included for comparison. Anthropometric variables and serological parameters were assessed. Height, weight, OI, body mass index (BMI) were significantly higher in obese group than in control. Obese children showed significantly higher serum concentrations of triglyceride (TG), total cholesterol (TC), and LDL-cho1esterol and significantly lower HDL-cholesterol compared with control children, even though the values were within normal ranges for both groups. Concentrations of resistin and leptin were significantly higher in obese group than in control. Adiponectin and insulin levels were tended to lower in obese group even though the differences were not statistically significant. Resistin had significant positive correlation with OI and TG, and 1eptin with weight, OI, BMI, TG and TC. On the other hand, adiponectin showed significant negative correlations with height, OI and BMI. These finding showed that obese children had higher serum levels of resistin and leptin and lower adiponectin, and also these hormones had correlations with related factors of obesity, suggesting adipocyte-derived hormones has a role in child obesity.

• 대한임상검사과학회지
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• 제40권2호
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• pp.98-105
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• 2008

Colorectal carcinoma from various cancers is fourth ranked occurred to Korean. Due to western dietary life, this cancer has been increased continuously. Therefore, the further study will be needed to find a candidate gene involved in the development and progression of colorectal carcinoma as well as to diagnose and treatment helpfully. The purpose of this study was designed to find a carcinogenesis gene using microsatellite marker on chromosomes 7th and 20th from 30 colon cancer patients. The amplification was investigated in order of D20S97 57% (17/30), D20S101 57% (17/30), D20S119 53% (16/30), D7S483 50% (15/30), D7S495 47% (14/30), D7S498 47% (14/30). The genetic mutation pattern depends on loci of colorectal carcinoma was shown highly amplified with 3.77 from colon cancer than with 2.08 from right colorectal carcinoma (P<0.018). The genetic mutation with lymph nodes was investigated higher with 4.13 at metastasized group than with 1.93 at non-metastasized group (P<0.001). There was no difference at comparison between histological classfication and serological CEA increase as well as on genetic mutated pattern depends on disease stage. It is suggested that the amplification on chromosomes 7q and 20q determines a pivotal role from first stage to metastasis cancer and also functions as an useful marker on diagnosis and treatment of colorectal carcinoma patients as well as follow-up checkup. Recently, the diagnosis and study using genetic analyzer are necessary for efficient application. Fortunately, several university hospitals run this genetic analyzer currently so it is expected that this method makes full use of clinical application.

• 대한의생명과학회지
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• 제10권3호
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• pp.253-257
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• 2004

The mechanisms by which leptospires caused disease are not well understood. A number of putative virulence factors have been suggested, but with few exceptions their role in pathogenesis remains unclear. In these days, many cases of leptospirosis are diagnosed by serological immunoassay. Leptospirosis is characterized by the histopathology of liver, kidney, heart, and lung, but the electrophoresis fractions of serum protein are not compared. We analyzed total protein, albumin, aspartic aminotranferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), creatinine and urea nitrogen (UN) in sera of patients diagnosed leptospirosis. Total protein and albumin were decreased in 18.5％ and 31.2％ of patients, respectively. AST, ALT, ALP, UN and creatinine were increased in 90.4％, 66.9％, 28.0％, 15.9％ and 10.8％ of patients, respectively. We performed cellulose acetate membrane electrophoresis (EP) on sera of patients increased both of AST and ALT, and of patients increased both of creatinine and UN. In patients increased both of AST and ALT, and in patients increased both of AST and ALT, the relative percentage of albumin to total protein in patient serum was dcreased in 89.1 ％ of patients. α₁-globulin, α₂-globulin, β-globulin and γ-globulin were increased in 85.1 ％, 75.2％, 33.6％ and 98.0％ of patients, respectively. In patients increased both of creatinine and UN, the relative percentage of albumin to total protein in patient serum was dcreased in 93.8％ of patients. α₁-globulin, α₂-globulin, β-globulin and γ-globulin were increased in 87.5％, 100％, 31.2％ and 93.8％ of patients, respectively. These data indicate that infection of Leptospira causes severe liver damage to most of leptospirosis patients, but doesn't cause renal damage to most of them. The relative rate of serum protein electrophoresis fractions to total protein are not identical with them of typical hepatitis patient.

• The Plant Pathology Journal
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• 제27권4호
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• pp.372-377
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• 2011

A virus causing mottle and stunt symptom on Zea mays was observed around Ulleng-do, Korea and identified as Cucumber mosaic virus (CMV-ZM) based upon biological, serological, and molecular characteristics. In host range studies, the CMV-ZM isolate produced local lesions on Datura stramonium, Vigna unguiculata, Cucurbita moschata, Chenopodium amaranticolor, Ch. quinoa, whereas this isolate produced systemic mosaic on Nicotiana tabacum cv. 'Xanthi-nc', Capsicum annuum, Solanum lycopersicum, Solanum melongena, Cucurbita pepo, and Z. mays. In addition, chlorotic local rings on inoculated leaves along with severe mosaic, malformation, and fern leaf symptoms on upper systemic leaves were shown in N. glutinosa plants. Complete nucleotide sequences of each genomic RNA segment was determined and compared to those of the other CMV strains. Comparison of the nucleotide sequence of 1a open reading frame (ORF) revealed approximately 89.2-92.4% sequence identity with each CMV subgroup IA and IB strain, while showing only 78% sequence identity with CMV subgroup II. Nucleotide sequence analysis of RNA2 ORFs revealed 85.3-97.6% sequence identity with subgroup I. In ORFs of RNA3, levels of nucleotide sequence identities were higher than 92-99.2% with CMV subgroup I and lower than 82% with CMV isolates of subgroup II. These results suggest that CMV-ZM isolate is more closely related to subgroup I than subgroup II and therefore, CMV-ZM isolate might be classified into as CMV subgroup I based on biological and molecular analysis.

• 대한수의학회지
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• 제43권3호
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• pp.471-476
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• 2003

Malignant catarrhal fever (MCF) is a systemic disease of ruminants caused by ovine herpesvirus 2 (OvHV-2). OvHV-2 is a gamma herpesvirus, which induces frequent latent infection and often difficult to detect its antigens and even specific nucleic acids because of its low viral copies in the infected tissues. Histopathology, serology and polymerase chain reaction (PCR) were compared for the diagnosis of MCF using 10 bison infected with OvHV-2. Histopathological diagnosis was performed using the criteria which was based upon the pathognomic lesions. Serological diagnosis was conducted using its serum with competitive ELISA for the detection of antibodies of OvHV-2. Also, the nest PCR was performed with peripheral blood leukocytes for the detection of OvHV-2-specific DNAs. Primers 556 and 775 were used for the primary amplification, and primers 556 and 555 were used for the secondary amplification. As the results, positive cases were 6 by histopahology, 9 by serology and 10 by PCR. As comparing with other diagnostic methods, PCR was found to be more sensitive than histopathology and serology. The recent development of molecular diagnostic assays has provided powerful tools for investigating how viruses survive in nature. Development of PCR specific for viruses has dramatically improved the accuracy of diagnosis of viruses in clinically infected animals. Furthermore, amplification of viral genomic material by nest PCR represents the most sensitive method for the detection of viruses and might be detected successfully even though very low viral DNA copies. So, it could be used as the first choice for the detection of viral DNAs with low copies such as the status of latent infection. However, it has also some limitation of application like as false negative results by PCR inhibitors and false positive results by contamination. The results of this study suggest that the use of molecular biological methods like PCR may increase the accuracy for the diagnosis of infectious diseases. However, in diagnostic laboratory, it is recommended that PCR assay must be conducted with other diagnostic methods for more reliable diagnosis.

• Clinical and Experimental Pediatrics
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• 제54권10호
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• pp.409-413
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• 2011

Purpose: Few studies have been conducted on the recent status of infectious mononucleosis (IM) in Korean children. The aim of this study was to evaluate the recent trend in the clinical manifestations of Epstein-Barr virus (EBV)-associated IM as well as the clinical differences according to age. Methods: A retrospective study was performed on 81 children hospitalized with EBV-associated IM who fulfilled the serological criteria for the diagnosis of EBV infection (viral capsid antigen immunoglobulin M positive). The patients were divided into 3 age groups: <5 years, 5 to 9 years, and ${\geq}10$ years. We evaluated the recent trend in clinical manifestations and the differences in clinical and laboratory findings among the 3 age groups. Results: Thirty (37%) children were under 5 years of age, 38 (46.9%) were 5 to 9 years of age, and 13 (16%) were 10 years of age or older. The differences in the symptoms and signs among the 3 age groups were not statistically significant, except for headache. The mean duration of fever was 7.7 days (range, 0 to 18 days). A comparison of liver enzyme elevation among the age groups showed an association with advancing age (26.6%, 63.1%, and 76.9%, respectively, P=0.04) Conclusion: This study showed that EBV-associated IM in Korean children continues to occur mostly in children under 10 years of age. In children with EBV-associated IM, the incidence of headache and liver enzyme elevation, the duration of fever, and the proportion of females to males were all positively associated with advancing age.