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Inhibitory effect on Streptococcus mutans and mechanical properties of the chitosan containing composite resin

  • Kim, Ji-Sun;Shin, Dong-Hoon
    • Restorative Dentistry and Endodontics
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    • v.38 no.1
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    • pp.36-42
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    • 2013
  • Objectives: This study evaluated the antibacterial effect and mechanical properties of composite resins ($L_{CR}$, $M_{CR}$, $H_{CR}$) incorporating chitosan with three different molecular weights (L, Low; M, Medium; H, High). Materials and Methods: Streptococcus (S). mutans 100 mL and each chitosan powder were inoculated in sterilized 10 mL Brain-Heart Infusion (BHI) solution, and was centrifuged for 12 hr. Absorbance of the supernatent was measured at $OD_{660}$ to estimate the antibacterial activities of chitosan. After S. mutans was inoculated in the disc shaped chitosan-containing composite resins, the disc was cleansed with BHI and diluted with serial dilution method. S. mutans was spread on Mitis-salivarius bacitracin agar. After then, colony forming unit (CFU) was measured to verify the inhibitory effect on S. mutans biofilm. To ascertain the effect on the mechanical properties of composite resin, 3-point bending and Vickers hardness tests were done after 1 and 3 wk water storage, respectively. Using 2-way analysis of variance (ANOVA) and Scheffe test, statistical analysis was done with 95% significance level. Results: All chitosan powder showed inhibition effect against S. mutans. CFU number in chitosan-containing composite resins was smaller than that of control resin without chitosan. The chitosan containing composite resins did not show any significant difference in flexural strength and Vickers hardness in comparison with the control resin. However, the composite resin, $M_{CR}$ showed a slightly decreased flexural strength and the maximum load than those of control and the other composite resins $H_{CR}$ and $L_{CR}$. Conclusions: $L_{CR}$ and $H_{CR}$ would be recommended as a feasible antibacterial restorative due to its antibacterial nature and mechanical properties.

Evaluation of Skeletal Stability Following Two-jaw Surgery via Surgery First Orthodontic Treatment in Class III Malocclusion (III급 부정교합에서 선수술 교정치료를 통한 양악 수술 후 안정성)

  • Hwang, Dae-Seok;Kim, Yong-Il;Lee, Jae-Yeol;Lee, Seong-Tak;Kim, Tae-Hoon;Lee, Joo-Min;Ahn, Kyung-Yong
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.33 no.5
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    • pp.407-412
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    • 2011
  • Purpose: The purpose of the present study was to evaluate the postoperative skeletal stability of two-jaw surgery (Le Fort I osteotomy and bilateral sagittal split ramus osteotomy) via surgery first orthodontic treatment (SFOT) in class III malocclusion. Methods: Thirty-two patients who had two-jaw surgery via SFOT were included in this study. Serial lateral cephalograms were obtained before (T0), immediately after (T1), and six months after (T2) surgery. Twelve variables were measured for horizontal and vertical skeletal stability as well as for dental change. All measurements were evaluated statistically by a paired t-test ($P$ <0.05). Results: The mean skeletal changes were $0.1{\pm}2.5$ mm at point A and $-12.0{\pm}7.4$ mm at the pogonion. The mean horizontal relapse was 11.6% at the pogonion, and the mean vertical surgical changes included an upward displacement of $2.1{\pm}7.1$ mm and a forward displacement of $1.4{\pm}4.6$ mm at the pogonion. Upper incisor inclination decreased after surgery and was maintained at T2, and lower incisors were proclined from T1 to T2 by postsurgical orthodontic treatment. Conclusion: Postoperative skeletal stability of two-jaw surgery via surgery first orthodontic treatment in class III malocclusion was clinically acceptable.

Osteomyelitis following Domestic Animal Bites to the Hand: Two Case Reports and Practical Guidelines

  • Lim, Jung Soo;Byun, Jin Hwan;Min, Kyung Hee;Lee, Hye Kyung;Choi, Yun Sun
    • Archives of Plastic Surgery
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    • v.43 no.6
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    • pp.590-594
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    • 2016
  • Recently, the number of cases of animal bite wounds has increased significantly in concordance with an increase in the pet population around the world. The authors report two rare cases of osteomyelitis of the phalanx following cat and dog bites. On initial physical examination, signs of a severe infection were observed. Radiographs of both patients showed the presence of osteomyelitis, and in one of the patients, the diagnosis was confirmed with a bone biopsy. After use of empirical antibiotics, intravenous antibiotic therapy that matched the identified bacterium's sensitivity was initiated, and at the same time, secure dressing including debridement was performed to induce secondary healing. In addition, the patients were closely monitored with serial X-rays, and culture and blood test follow-up. One patient fully recovered without sequelae, but the other patient suffered a loss of distal interphalangeal joint motion. When dealing with bite wounds located on the hand, it is important to visit the hospital as soon as possible and receive the appropriate treatment early. Moreover, to prevent severe complications such as osteomyelitis, it is important to administer antibiotic therapy to which the cultured bacteria are sensitive, along with proper wound management and prophylactic antibiotic treatment.

A Case of Pulmonary Endometriosis Treated by Resection (폐절제술로 치료한 폐 자궁내막증 1예)

  • Park, Sang-Mi;Shin, Eun Jung;Kang, Kyung Mi;Kim, Min Kuk;Cho, Deog Gon;Song, So Hyang;Kim, Chi Hong
    • Tuberculosis and Respiratory Diseases
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    • v.61 no.4
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    • pp.394-397
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    • 2006
  • Periodic hemoptysis occurring at the time of menstruation (catamenial hemoptysis) is a rare disorder and generally signifies indicates the presence of thoracic endometriosis. A diagnosis of catamenial hemoptysis is usually established based on the clinical history and the exlusion of other causes of the recurrent hemoptysis. Serial computed tomograms of the chest during and in the interval between menstruations periods is a useful confirmatory test. We report the case of a 22 year-old women who suffered from catamenial hemoptysis for 10 months and was treated successfully with a wedge resection of the lesion by video-assisted thoracoscopic surgery(VATS). There was no evidence of recurrence. In conclusion. VATS is a good choice as a for single focus for catamenial hemoptysis.

Time course of the denervation in early stage of Bell's palsy.: Identification by electrophysiologic study (초기 벨마비에서 나타나는 탈신경의 시간경과에 따른 변화: 전기생리학적 검사를 통한 확인)

  • Bae, Jong-Seok;Uhm, Keun-Yong;Kim, Byoung-Joon;Kwon, Ki-Han
    • Annals of Clinical Neurophysiology
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    • v.6 no.1
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    • pp.26-30
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    • 2004
  • Background: Electrophysiologic study accurately predicts the degree of degenerated motor axons but cannot give precise information on the type of injury that occurred in Bell's palsy. Because of these limitation for prognostic prediction in Bell's palsy, we evaluated divergence of electrophysiological time course for the purpose of presuming the type of injury in Bell's palsy. Methods: We did bilateral facial nerve conduction studies in 103 Bell's palsy patients, who visited to Han-Gang sacred heart hospital from 1998 to 2001. We compared the CMAP amplitude of disease site with that of normal site and suggested that decremental CMAP amplitude ratio (percentage) as a degree of denervation of affected facial nerve. Then we demonstrated the time course of denervation percentage. After defining normal range of CMAP amplitude difference from normal control group, we also evaluated if distinct time course of early minimal denervation is present. Results: Our results show that time course of the denervation in early stage of Bell's palsy reflect various injury type such as axonotmesis, neurotmesis or other unidentified type. We cannot identify the distinct time course of early minimal denervation. Conclusions: The time course as well as the maximal value of denervation are the best prognostic guidelines in Bell' s palsy. So repeated serial electrophysiologic test are inevitable to assess prognosis. As an another topic, early minimal denervation for prognostic prediction deserve to be evaluated as a future work up for prognostic prediction.

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Simulation for the Fuel Economy and the Emission of Diesel Hybrid Electric Vehicle (디젤 하이브리드 전기 자동차의 연료경제성 및 배출가스에 관한 시뮬레이션)

  • Han, Sung-Bin;Chang, Yong-Hoon;Suh, Buhm-Joo;Chung, Yon-Jong
    • Journal of Energy Engineering
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    • v.18 no.1
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    • pp.31-36
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    • 2009
  • There are several types of environment friendly vehicle being developed by auto manufactures. HEV (Hybrid Electric Vehicle) is most applicable one among them in actuality. HEV has two power sources, one is an internal combustion engine, the other one is an electric device. The HEV is developed for reducing fuel consumption and emissions. We selected the diesel engine as a main power source of HEV. The tests were carried out under different driving cycles which was CBDBUS (Central Business Driving Bus Schedule) and HWFET (Highway Fuel Economy Test). This research presents a simulation for the fuel economy and the emission of heavy diesel hybrid vehicle according to the SHEV (Serial Hybrid Electric Vehicle), PHEV (Parallel Hybrid Electric Vehicle), Plug-in SHEV and plug-in PHEV.

Studies on the Antifungal Antibiotics Produced by a Streptomyces sp. (Part 4) The Occurrence of Tetraene Substance and Its Physiological Properties (Streptomyces sp. 가 생산하는 항진균성 항생물질에 관한 연구 (제4보) Tetraene계 항진균성 항생물질의 생성및 그의 성장)

  • Ko, Young-Hee;Bae, Moo
    • Microbiology and Biotechnology Letters
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    • v.10 no.3
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    • pp.211-215
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    • 1982
  • Streptomyces griseorubiginosus var. soyoensis previously identified, produced two kinds of antifungal antibiotics, trans-cinnamamide and another new substance. The latter was identified to be a new substance of tetraene family by establishment of UV, IR, NMR, mass spectra and chemical reactions and rotatively named as Tetraene KM-A. Through an antimicrobial activity test using serial agar dilution method, Tetraene KM-A showed strong growth inhibitory activity against fungi and yeasts, but not against procaryotes tested. The inhibitory action of Tetraene KM-A on fungi was remarkably ineffective when some of sterols were added to the cultural media. $LD_{50}$ of the Tetrene KM-A to mice and rats by intravenous injection were 84.3 and 90.4 mg/kg respectively. $LD_{50}$ to mice by oral feeding was 1503mg/kg.

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Development of Power Management System for Efficient Energy Usage of Small Generator (소형 발전기의 에너지 절약을 위한 전력관리 시스템 개발)

  • Jeon, Min-Ho;Oh, Chang-Hyun
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.16 no.12
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    • pp.2601-2606
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    • 2012
  • In this paper, an electricity management system, which saves energy by utilizing electricity consumption of load from an environment that uses at least two compact generators, is proposed and developed. A hardware is constructed by using TMS320C6713 DSP chip made by TI that is capable of high speed hardware floating point processing while serial communication is used for communication with a monitoring PC. Manual control is made possible from the monitoring PC and automatic on/off is enabled in the generator by using data collected by CT/PT sensor from the DSP mainboard. Test results confirm that the electricity management system proposed in this study functions without abnormality. The application of an algorithm that saves energy by using electricity consumption of load also allows for a longer supply of electricity compared to continuously using two compact generators.

Effects of Oral Administration of Ligigeopoongsan on Neuropathic Pain and c-Fos Protein Expression in Rats (이기거풍산(理氣祛風散)이 백서(白鼠)의 신경병리성(神經病理性) 동통(疼痛) 억제(抑制) 및 c-Fos 단백(蛋白) 발현(發顯)에 미치는 영향(影響))

  • Lee, Hyek-Je;Kim, Jong-Han;Park, Su-Yeon;Choi, Jung-Hwa
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.18 no.1
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    • pp.50-60
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    • 2005
  • Objective : We have studied to know effects of Ligigeopoongsan(LGS, 理氣祛風散) on mechanical allodynia, cold allodynia and c-Fos protein expression in the model of neuropathic pain of rats. Methods : The model of neuropathic pain was made by injured tibial nerve and sural never while common peroneal never was maintained. After 2weeks, we performed behavioral test for 7 days to try out mechanical allodynia using von frey filament and cold allodynia using acetone, which are calculated by counting withdrawal response on foot. Rat brains removed and sliced on 8th days. Serial sections were immunohistochemically reacted with polyclonal c-Fos antibody. The numbers of c-Fos protein immunoreactive neurons in the central gray were examined using scion image program. Results : 1. Mechanical allodynia in LGS-2, LGS-3 groups were significantly diminished compared with the control group. 2. Cold allodynia in LGS-3 group was significantly diminished compared with the control group. 3. c-Fos protein expression on the central gray LGS-2, LGS-3 groups were significantly lower than that of control group. conclusions : We have noticed that LGS(理氣祛風散) diminished mechanical and cold allodynia in the model of neuropathic pain compared with the control group. c-Fos protein expression in the central gray of that group was also decreased compared with the control group. Pain control group were LGS was accumulated time goes by. This study can be used as a basic resource on a study and a treatment of pain.

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A Study on the Water Quality Relationship between Continuous Dam Discharge and Downstream in North Han River (북한강에 연속된 댐 구간 방류수와 하류 하천간 수질 관계 분석 연구)

  • Kim, Ji Won;Lee, Hye Won;Lee, Yong Seok;Choi, Jung Hyun
    • Journal of Korean Society on Water Environment
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    • v.36 no.2
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    • pp.89-97
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    • 2020
  • North Han River is a very unique type of water system, where Hwacheon, Chuncheon, Soyanggang, Euiam and Cheongpyeong Dams are located consecutively. These dams are operated differently in the amount of discharge and release schedule according to their structure and purpose of use. They have different water quality characteristics depending on external pollutant inflow and internal mixing condition. Therefore, this study investigated the relationship between the upper dam and down stream river with respect to water quality indicators, such as water temperature, electrical conductivity, BOD, COD, TN and TP of the North Han River. The similarities and correlations representing the relationship were analyzed by Pearson's correlation r and t-test. The data was taken from the Ministry of Environment's water quality monitoring from 1999 to 2018. The results show that water temperature and electrical conductivity of the dam and river are similar and correlated. However, it turned out that there was no similarities and correlations in BOD, COD, TN and TP that are significantly affected by subaqueous reaction mechanism. The results of this study present the impact of the dam on the water quality of North Han River, which can be used as useful data for management of water quality.