• 제어로봇시스템학회:학술대회논문집
• /
• 1990.10a
• /
• pp.406-410
• /
• 1990

This paper presents an approximate analysis of the serial production lines using decomposition technique. A serial production line consists of a series of unreliable machines separated by finite buffers. The serial production line is evaluated by approximation method, the results of which are compared with those examined by the discrete time event simulation, based on this approximation method, a gradient technique is proposed, which improves the efficiency of an operation of production line through the re-allocation of buffers.

• 제어로봇시스템학회:학술대회논문집
• /
• 1995.10a
• /
• pp.259-262
• /
• 1995

In this paper, a model of an asymptotically reliable serial production line with quailty control devices is introduced and analyzed. By an asymptotic technique and Taylor series expansion, its average production rate is approximated in a closed form. The results are applied to a case study of a surface mount system.

• 제어로봇시스템학회:학술대회논문집
• /
• 1996.10a
• /
• pp.350-353
• /
• 1996

In this paper, based on the performance analysis of serial production lines with quality inspection machines, we develope an buffer size optimization method to maximize the production rate. The total sum of buffer sizes are given and a constant, and under this constraint, using the linear approximation method, we suggest a closed form solution for the optimization problem with an acceptable error. Also, we show that the upstream and downstream buffers of the worst performance machine have a significant effect on the production rate. Finally, the suggested methods are validated by simulations.

• Journal of the Korean Institute of Telematics and Electronics B
• /
• v.30B no.8
• /
• pp.23-32
• /
• 1993

This paper presents a decomposition method to evaluate the performance measures of the serial production lines with unreliable machines and finite buffers. This method is to decompose the transfer line into a set of two machine lines for analysis. Bases on this approximation method, we consider nonhomogeneous lines. In such lines, the machines may take the different lengths of time performing operations on parts. A new transformation is employed in order to replace the line by a homogeneous line. The approximate transformation enables one to determine parameters of performance such as production rate and average buffer levels, and it also shows better application for a large class of systems.

• 제어로봇시스템학회:학술대회논문집
• /
• 1990.10a
• /
• pp.519-522
• /
• 1990

This paper formulates a problem of analysis and design of serial production lines, closed with respect to the number of carriers available in the system for parts transportation between operations. For two machines - two buffers systems, the paper gives an asymptotic solution and shows that optimization of the system with respect to the number of carriers available and the capacity of the feedback buffer may lead to substantial improvements of system's performance.

• Journal of the Korea Society for Simulation
• /
• v.19 no.2
• /
• pp.107-118
• /
• 2010

Automotive manufacturing is a complex task that requires the production and assembly of thousands of different components or parts. The engine and the transmission are the major components that constitute a power train system. Although manufacturing processes of an engine are similar, the layouts of the manufacturing lines are different from factory to factory. It is due to the different design concept that how to combine the serial and parallel structures. In this paper, three engine lines of different factories are introduced, and the simulation technology is used to make the performance analysis for different design concepts.

• 제어로봇시스템학회:학술대회논문집
• /
• 1992.10b
• /
• pp.458-462
• /
• 1992

This paper presents a decomposition method to evaluate the performance measures of transfer line with unreliable machine and finite buffers. The proposed method is to decompose the transfer fine into a set of two machine lines for analysis. Nonhomogeneous lines are considered. In such fines, the machines may take the different lengths of time performing operations on parts. A simple transformation is employed in order to replace the line by a homogeneous line. The approximate transformation enables one to determine parameters of performance such as production rate and average buffer levels, and it also shows better applications for a large class of systems.

• Journal of Microbiology and Biotechnology
• /
• v.28 no.6
• /
• pp.997-1006
• /
• 2018

As shown during the 2009 pandemic H1N1 (A(H1N1)pdm09) outbreak, egg-based influenza vaccine production technology is insufficient to meet global demands during an influenza pandemic. Therefore, there is a need to adapt cell culture-derived vaccine technology using suspended cell lines for more rapid and larger-scale vaccine production. In this study, we attempted to generate a high-growth influenza vaccine strain in MDCK cells using an A/Puerto/8/1934 (H1N1) vaccine seed strain. Following 48 serial passages with four rounds of virus plaque purification in MDCK cells, we were able to select several MDCK-adapted plaques that could grow over $10^8PFU/ml$. Genetic characterization revealed that these viruses mainly had amino acid substitutions in internal genes and exhibited higher polymerase activities. By using a series of Rg viruses, we demonstrated the essential residues of each gene and identified a set of high-growth strains in MDCK cells ($PB1_{D153N}$, $M1_{A137T}$, and $NS1_{N176S}$). In addition, we confirmed that in the context of the high-growth A/PR/8/34 backbone, A/California/7/2009 (H1N1), A/Perth/16/2009 (H3N2), and A/environment/Korea/deltaW150/2006 (H5N1) also showed significantly enhanced growth properties (more than $10^7PFU/ml$) in both attached- and suspended-MDCK cells compared with each representative virus and the original PR8 vaccine strain. Taken together, this study demonstrates the feasibility of a cell culture-derived approach to produce seed viruses for influenza vaccines that are cheap and can be grown promptly and vigorously as a substitute for egg-based vaccines. Thus, our results suggest that MDCK cell-based vaccine production is a feasible option for producing large-scale vaccines in case of pandemic outbreaks.