• Applied Biological Chemistry
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• v.13 no.1
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• pp.65-72
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• 1970

Two peptides (Im pr-M, Im ch-M) derived from Im ${\lambda}-type$ of Bence Jones Protein and one peptide (Ikch-M) from Ik were separated and purified using the Dowex $50{\times}2$ column $(1{\times}20\;cm)$ and Dowex $1{\times}2(0.9{\times}50\;cm)$. The buffer solution was composed of 1% pyridine and IM formic acid in Dowex $1{\times}2$ column. The blocked N-terminal was examined with ninhydrin reaction before and after alkaline hydrolysis, which was fractionated by Dowex $1{\times}2$ column. Pyrro-glutamic acid in N-terminal residue was identified by comparing with the authentic pyrro-glutamic acid through a high voltage electrophoresis (pH 3.5, 3000 V.) after the peptide Im pr-M (PCA. Ser) was cleavaged at the position of serine with cone. (12 N) HCl and the pyrro-glutamic acid was converted to glutamic acid by treating it with N-NaOH for 116 hours at $27^{\circ}C$. The substractive method was applied to find out the sequence of peptides and carboxypeptidase A was employed to release C-terminal residue from the peptide. In present study PCA. Ser in Im Pr-M was isolated from the pronase digested ${\lambda}$-type Bence Jones protein. The yield of the Im Pr-M was 79.6 percent of its theoretical value, based on the molecular weight of Bence Jones Protein. Im ch-M (PCA. Ser Val. Leu) was isolated from the chymotrypsin digested ${\lambda}$-type Bence Jones Protein. The yield of the Im ch-M was 72.2 percent. based on the molecular weight of Bence Jones Protein. Ik ch-M (PCA. Ser. Ala. Leu) was isolated from the chymotrypsin digested ${\lambda}$-type Bence Jones Protein and its yield was 42% based on the molecular weight of Bence Jones Protein.

• Physical Therapy Korea
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• v.19 no.2
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• pp.20-28
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• 2012

The lumbar multifidus muscle, which can be separated into deep fascicles (DM) and superficial fascicles (SM), is important for lumbar segmental stability. However, no previous studies have investigated the effect of lumbar stabilization exercises on the thickness of DM and SM. Thus, the purpose of this study was to assess DM thickness after three different lumbar segmental stabilization exercises. In total, 30 healthy male participants were recruited and randomly assigned to one of three exercise groups: hollowing in the quadruped position (H-Quad), contralateral arm and leg lift (CALL), and bilateral arm and leg lift (BALL). Each lumbar segmental stabilization exercise was conducted over 4 weeks. Ultrasonography was used to compare the DM and SM thickness before and after the 4 weeks of exercise. A mixed-model analysis of variance using Scheffe's post-hoc test was used for statistical analysis. The results showed a significant effect for the measurement time (before vs. after 4 weeks of exercise) in the DM (F=31.26, p<.05) and SM (F=4.56, p<.05). At the end of the 4 weeks, the DM thickness had increased significantly in the H-Quad exercise group, and the SM thickness had increased significantly in the CALL and BALL exercise groups. Also in the BALL exercise group, the SM thickness was greater compared with that in the H-Quad exercise group. These findings suggest that the thickness of the DM and SM were increased by different types of lumbar segmental stability exercise after 4 weeks.

• Journal of Korean Society of Forest Science
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• v.79 no.3
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• pp.330-342
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• 1990

One might have a retrospect on the relationship between the forest and human being from the viewpoint of ecological perspective. It is no doubt that most of the fossil humans should have lived on the forest and the latter provided foods and shelters for humans from their beginning stages, Since the so-called agricultural revolution, humans have extensively started to exploit the forest which had beer, their cradle. The industrial revolution has created another situation against the forest in terms of the quality of ecosystem. These two revolutions have set up the so-called civilization which seems to have been based on the sacrificial oblation of the forest. The cradle for human being has been kept exterminating for the shake of "economic development and miracle." This might be a synoptic history of relationships between the forest and human beings in a sense. designates the behavioral aspects of human being against the forest and people consider the forest only as exploitable resource in this context, and the latter means that people live on the forest and strive to adapt the order of forest ecosystem. The resourcism has developed a strategy of colonialism to exploit the forest and provided a winner's position for the human beings against the forest, This idea and behavioral perspective seems to have started the backfire against the exploiter who is the owner of the civilization. However, there are different philosophies and ideas to view the relationship between the forest and human beings. People within the forest who are mostly considered as "primitives" still keep their idea of the ontology of the forest. There is a theoretical assumption of the "socionatural system" to look into the ecosystem. The forest could be viewed in the above frame of analysis. There are five variables : environment, resource, technology, organization, and ideology. Ideological aspect of the forest can be explained in the context of belief systems. Forest has a meaning of religion and rituals and people within the forest should admire it in anyway of religious reasons. This aspect of the forest cannot be separated from the environmental aspect of the forest. People within the forest acknowledge and practice the above idea. People outside the forest have lost the idea, however, at the cost of acquiring the civilization. They have expelled themselves from the forest and divided the socionatural system of the forest by way of colonialism. The efforts like agroforestry and social forestry would be strategies for recovering the idea of ontology of the forest as well as the sense of community including the forest and human being. People within the forest will be a prospective model for the future socionatural system of the forest for the people outside the forest. At this point, an ecological anthropologist can work with the forest specialists.

• Korean Journal of Plant Taxonomy
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• v.35 no.2
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• pp.81-98
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• 2005

Leaf morphology was examined for Prunus species from Jeju Island. Analyzed were the leaf blade length, petiole length, width of blade, number of veins and angle of the base to the mid-vein as quantitative characters and the distribution of trichome, position of glandsand leaf features qualitative characters. A total of 25 OTU were phenetically analyzed by UPGMA. The resuling phenograms slightly differ from the currently recognized taxonomic system in two points. Prunus mume was clustered with P. padus and P. buergeriana. Prunu spendula and P. yedoensis were separated from the cluster of P. jaamasakura, P. speciosa, P. sargentii and P. jamasakura var. quelpaertensis. Except for the members of subgenus Cerasus, subgenera Padus (P. buergeriana and P. padus), Microcerasus (P. japonica) and Amygdalus were well defined. Some morphological characters of leaves such as the ratio of blade length to width, the length of blade to petiole, number of veins, the distance between the gland and base, the angle of base to mid-vein, and the distribution of trichome were useful as diagnostic features for Prunus from Jeju Island.

• Food Science and Preservation
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• v.15 no.3
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• pp.437-444
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• 2008

Structured lipids(SLs) were synthesized by enzymatic interesterification with DHA-enriched fish oil(containing 27% docosahexaenoic acid) and soybean oil in the hatch-type reactor. The interesterification was performed for 24 hr at $55^{\circ}C$ and TLIM(immobilized lipase from Thermonyces lanuginosa, 10% by weight of total substrates) was mixed with 180 rpm of shaking. The fish oil and soybean oil were interesterifed with several weight ratio(fish oil : soybean oil, 2:8, 3:7, 4:6, 5:5, w:w), Reverse-phase high performance liquid chromatography with an evaporative light-scattering detector separated the triglyceride species of SLs. The products contained the newly synthesized peaks. Especially, one of peaks was distinctively increased with the increasing weight ratio from 2:8 to 5:5 while the peak of trilinolein (LLL) decreased vice versa. The effect of antioxidants such as catechin, BHT(Butylated hydroxytoluene), and their combinations on the oxidative stability in SL were investigated. Oxidative stability was carried out under oven test at $60^{\circ}C$ over 72 hr thereafter SLs were analyzed for total fatty acid content, rancimat, peroxide value, electronic nose and TBARS value. Among all combinations of antioxidant, the highest stability was obtained from 200 ppm of catechin. Besides, total tocopherol ($\alpha$, $\gamma$, and $\delta$-tocopherol), iodine and saponification value were analyzed in which iodine and saponification value of SLs were 151.19 and 182.35.

• Journal of Korea Port Economic Association
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• v.30 no.4
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• pp.219-254
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• 2014

Due to the recent development of globalization and supply chain management, growth of international trade has led to increasing cargo volume around the world. Since maritime logistics environments have changed, increased container ship size, improvement of harbor equipment, global port operation and rapid technological development have had an significant effects on shipping and port industry, which is contributing to competitiveness of port. Since a larger volume of port throughputs are generally regarded as an indicator of the more competitive port, inefficient port operation could reduce its competitiveness. On the other hand, high efficient ports could increase their competitive power while increasing cargo volume. This study aims at comparing competitiveness of the ports in the Northeast Asia by investigating changes of container throughputs and evaluating efficiency performance of ports. Shift-Share analysis and Data Envelopment Analysis(DEA) have been conducted with 21 Northeast Asian ports and then separated them into 4 groups for comparative analysis to identify competitive position of each port. The results of this study show that Incheon and Gwangyang port have been decreased container cargo volume, while volume of Busan port would increase by means of active marketing, various route development and incentive policy for the port.

• Journal of the Korean Society of Clothing and Textiles
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• v.21 no.4
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• pp.769-781
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• 1997

The purpose of this research is to show how to use the patternist's CAD system in order to make design patterns efficiantly. Using the PAD System Technologies company's PAD System Module, which is very officiant in designing patterns, the basic pattern or design pattern can be designed and applyed to the Sample Module, thus, then it could manufacture, retouch or choose a model, making it posible to go to the next level which is seam patterns for industrial use. By understanding the change of the design that is chosen through the design pattern making and Sample Module process', this study is presentated a plan on how to utilize the patternist's working adaption ability by the CAD system, and also considered the problems in using it. Ability of Design Confrontation of CAD System: 1) The result of an operation can be saved and retouched. On a saved list the patterns can be chosen and changed, thus, making it posible to complete a design very quickly. 2) When sewing every designed pattern in Sample Module, the model can be operated merely by selecting each sewing position, thus creating the same effect of a designer's muslin sample process in a much lesser time. Point at issue in the utilization CAD system: 1) When combining two darts into one place the amounts of darts that are already these have to be erasers, and then, the two darts points are moved that place, thus, containing them, which is very conplicated. 2) When making a gathering, because system can only be recognized in a opend line, the pattern should be separated in order to put a gathering inside a pattern. 3) In order to readjust the amounte of dart. The dart is erased first and then made new dart again. 4) When making tucks in a Sample Module, the form that gets narrow towards the pivot point can not be recognized so the sewing lines of the tucks is adjusted into parallel lines. 5) The Sample Module 3D operation can not attach the bodice to the shirts, instead they become fixed into each other. In the case of shoulder line tucks the amounts of pleats can not be expanded draperary naturally, thus, making it hard to showing the silhouette accurately. If these problems are solved, especially if the silhouette of a model can be created to have natural drapery in the 3D Module, models can be shown on the screen in possible to compare and examine even a change of a detail, by designing this 3D Module Production sample process will be more effective, thus, making the CAD system more useful in sewing design process.

• Journal of Microbiology and Biotechnology
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• v.16 no.10
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• pp.1561-1569
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• 2006

Denaturing gradient gel electrophoresis (DGGE) is one of the most frequently used methods for analysis of soil microbial community structure. Unbiased PCR amplification of target DNA templates is crucial for efficient detection of multiple microbial populations mixed in soil. In this study, DGGE profiles were compared using different pairs of primers targeting different hypervariable regions of thirteen representative soil bacteria and clones. The primer set (1070f-1392r) for the E. coli numbering 1,071-1,391 region could not resolve all the 16S rDNA fragments of the representative bacteria and clones, and moreover, yielded spurious bands in DGGE profiles. For the E. coli numbering 353-514 region, various forward primers were designed to investigate the efficiency of PCR amplification. A degenerate forward primer (F357IW) often yielded multiple bands for a certain single 16S rDNA fragment in DGGE analysis, whereas nondegenerate primers (338f, F338T2, F338I2) differentially amplified each of the fragments in the mixture according to the position and the number of primer-template mismatches. A forward primer (F352T) designed to have one internal mismatch commonly with all the thirteen 16S rDNA fragments efficiently produced and separated all the target DNA bands with similar intensities in the DGGE profiles. This primer set F352T-519r consistently yielded the best DGGE banding profiles when tested with various soil samples. Touchdown PCR intensified the uneven amplification, and lowering the annealing temperature had no significant effect on the DGGE profiles. These results showed that PCR amplification bias could be much improved by properly designing primers for use in fingerprinting soil bacterial communities with the DGGE technique.

• Journal of Korean society of Dental Hygiene
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• v.5 no.2
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• pp.279-296
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• 2005

The purpose of this study was to examine what types of experiences dental hygienists underwent with patients who were unapproachable in an effort to find out the latter group's needs and expectations, explore how to treat them of different personality type, and provide better dental services in response to their needs. To attain the purpose, Q-methodology was employed, which made a subjective and systematic assessment of human subjectivity. There were three types of subjectivity among dental hygienists in conjunction with their experiences with patients who were hard to please. Each group whose subjectivity was different also had a different preference for patients, which was not exclusive to one another but unique. Type 1 was "avoiding patients who showed off". Dental hygienists of this type found it unpleasant to treat patients who boasted of their background, position or relations with the head of hospital and wanted to be given special treatment. They avoided those patients, since giving special treatment to specific patients was likely to do damage to others. They believed that better medical services could be provided through mutual concern and good manners between medical personnels and patients. Dental hygienists of type 2 considered it hard to treat patients who were picky and looked at treatment or its outcome negatively. Those who had to be separated from others on account of possible cross-infection or who called for special decontamination methods of dental instruments were also difficult to deal with. Dental hygienists of this type could be said to "avoid picky patients", as they preferred to fare with patients by offering good, faithful treatment rather than by giving special treatment. Dental hygienists of type 3 believed that smooth and successful treatment hinged on mutual trust, confidence and collaboration between medical personnels and patients. According to them, patients who choose a specific hospital or a particular medical team at their own option have to cooperate if necessary, listen carefully to medical personnels and treat them without any hostility or bias. Therefore, they could be said to "avoid patients who were not cooperative".

• Journal of the Korean Institute of Gas
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• v.7 no.4 s.21
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• pp.44-52
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• 2003

In this work dynamic heat transfer in a CPFS (cable penetration fire stop) system built in the firewall of nuclear power plants is three-dimensionally investigated to develop a test-simulator that can be used to verify effectiveness of the sealant. Dynamic heat transfer in the fire stop system is formulated in a parabolic PDE (partial differential equation) subjected to a set of initial and boundary conditions. First, the PDE model is divided into two parts; one corresponding to heat transfer in the axial direction and the other corresponding to heat transfer on the vertical planes. The first PDE is converted to a series of ODEs (ordinary differential equations) at finite discrete axial points for applying the numerical method of SOR (successive over-relaxation) to the problem. The ODEs are solved by using an ODE solver In such manner, the axial heat flux can be calculated at least at the finite discrete points. After that, all the planes are separated into finite elements, where the time and spatial functions are assumed to be of orthogonal collocation state at each element. The initial condition of each finite element can be obtained from the above solution. The heat fluxes on the vertical planes are calculated by the Galerkin FEM (finite element method). The CPFS system was modeled, simulated, and analyzed here. The simulation results were illustrated in three-dimensional graphics. Through simulation, it was shown clearly that the temperature distribution was influenced very much by the number, position, and temperature of the cable stream, and that dynamic heat transfer through the cable stream was one of the most dominant factors, and that the feature of heat conduction could be understood as an unsteady-state process.