• Journal of Microbiology and Biotechnology
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• 제13권6호
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• pp.1018-1020
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• 2003

The complete 1,486 nucleotide sequence of a cryptic plasmid separated from Lactobacillus bifermentans strain A02 isolated from Kimchi has been determined. The plasmid, designated as pA021, encodes a 33,488 Da putative Rep protein. Based on the sequence similarity, the protein shows homology with coding protein of pRS1, a previously reported plasmid of Oenococcus oeni and the replication initiation protein (Rep) of the Staphylococcal pT181 plasmid family.

• 한국주거학회논문집
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• 제13권2호
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• pp.65-71
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• 2002

Recently, the space organization of Korean-Chinese houses is different from that of other territories. The characteristics of eating and dinning space for Korean-Chinese Multi-family Housing are as follows ; ⅰ) The most obvious change in Korean-Chinese urban housing style was seen in kitchen space, which can be classify three types such as traditional type, improved type, and LDK type on the bases of heating and cooking method. ⅱ) Special feature of Korean-Chinese housing in Yanji city was that kitchen fulfilled important functions such as heating, cooking, dinning, sleeping, bathing and washing. Since then, each function was separated and functions of kitchen were cut down after all. iii) The arrangement of kitchen space have changeed according to the change of heating fuel, heating system, equipment, relatcd policy, etx. Multi-function kitchen discovered only in Yanji had lasted until 1980's by equipping heating floor system, which is suitable for sit-down living style. In 1990's, this kitchen style was prohibited legally, and the balcony area of kitchen widened due to the abolition of outdoor storehouse for foodstuff.

• 한국생활과학회지
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• 제12권4호
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• pp.403-416
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• 2003

The recent study was examined the effects of stress related to self-esteem upon the types of deficiency, the genders, the grades. A Questionnaire was distributed to 172 elementary school students in 4th, 5th and 6th grades. The results of this study were as follows : First, children's stresses were related to their self-esteem and their self-esteem was explained mostly by the stress from home environment. Second, children's stress upon the birth order was higher in the first than in the second and the third birth order. Third, their self-esteem was explained mostly by the support from friends, teachers, and academic fields. Forth, children's stress of divorced and separated families as a reason of deficiency was higher than their stress upon the separation by death.

• 가족자원경영과 정책
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• 제5권2호
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• pp.87-98
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• 2001

The study was to examine the effect on the thinking of environmental preservation and behavior of dietary life control on discharging behavior of food wastes in household. The data were obtained from 443 homemakers living in Seoul. The statistical techniques used for this study included descriptive statistics, ANOVA, and Multiple Regression Analysis. As the result of analyzing relative effects of factors on discharging behavior of food wastes, for the degree of separation and discharge of food wastes and general wastes, they further actively separated and discharged them when they lived in public house(apartment, villa, tenement house), they had direct charge of dietary life, they had further serious thinking of environmental preservation, and they lived further diligent lives.

• Computers and Concrete
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• 제14권2호
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• pp.145-161
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• 2014

The article presents the statistical method of grouping the results of the compressive strength of concrete in continuous production. It describes the method of dividing the series of compressive strength results into batches of statistically stable strength parameters at specific time intervals, based on the standardized concept of "concrete family". The article presents the examples of calculations made for two series of concrete strength results, from which sets of decreased strength parameters were separated. When assessing the quality of concrete elements and concrete road surfaces, the principal issue is the control of the compressive strength parameters of concrete. Large quantities of concrete mix manufactured in a continuous way should be subject to continuous control. Standardized approach to assessing the concrete strength proves to be insufficient because it does not allow for the detection of subsets of the decreased strength results, which in turn makes it impossible to make adjustments to the concrete manufacturing process and to identify particular product or area on site with decreased concrete strength. In this article two independent methods of grouping the test results of concrete with statistically stable strength parameters were proposed, involving verification of statistical hypothesis based on statistical tests: Student's t-test and Mann - Whitney - U test.

• 한국미생물·생명공학회지
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• 제31권4호
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• pp.429-434
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• 2003

Microbispora 속에 소속된 미생물 9주(표준 미생물 3종과 토양 분리주 6주를 포함) 에 대하여 16S ribosomal RNA 유전자 염기서열이 결정되었다. 이들은 Streptosporangiaceae과의 대표균주들의 염기서열과 비교하여 Microbispora 속에 소속된 미생물들의 진화적 유연 관계가 조사되었다. 계통분석 결과 Microbispora 속의 모든 표준 종들은 일관성 있게 단계통 단위를 형성하였으며 Streptosporangiaceae과의 다른 속들과 잘 구분되었다. 토양 분리주들은 모두 Microbispora속에 소속된 반면 비공식적으로 기술되어 온 Microbispora griseoalba IMSNU$ 22049^{T}$ (=KCTC $9314^{T}$ )는 Nocardia 속의 미생물들과 가까운 유연 관계를 보여 주었다.

• Toxicological Research
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• 제18권2호
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• pp.183-190
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• 2002

The mechanism by which catechin-mediated cytotoxicity against tumor cells remains to be elusive. To elucidate the mechanical mights of anti-tumor effects, (-)epigallocatechin-gallate (EGCG) of catechin was applied to human prostate cancer DU 145 cells. Cell viability was measured by crystal violet staining. Cell lysates were wed to measure the catalytic activity of caspases by using fluorogenic peptide: Ac-DEVD-AMC for caspase-3 protease, Z-IETD-AFC for caspase-8 protease, Ac-LEHD-AFC for caspase-9 protease as substrates. The equal amounts of protein from cell lysate was separated on SDS-PAGE and analyzed by western blotting with anti-Fas antibody, anti-FasL antibody, anti-BCL2 antibody and anti-Bax antibody. (-)EGCG induced the death of DUl45 cells, which was revealed as apoptosis shown by DNA fragmentation. (-)EGCG induced the activation of caspase family cysteine proteases including caspase-3, -8 and -9 proteases in DU145 cells. Also, (-)EGCG increased the expression of Fas and Fas ligand (FasL) protein in DU145 colls. The expression level of BCL2 was decreased in (-)EGCG treated DU145 cells, whereas Bax protein was increased in a time-dependent manner. We suggest that (-)EGCG-induced apoptosis of DU145 cells is mediated by signaling pathway involving caspase family cysteine protease, mitochondrial BCL2-family protein and Fas/FasL.

• 한국주거학회논문집
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• 제17권2호
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• pp.77-85
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• 2006

This study aims to propose the comprehensive evaluation of performance multi-family housing. Most of the existing studies for the reproduction have evaluated by the safety of structure. Therefore those pass over the functional factors which is important for the evaluation of residential qualities. This study represents that the reproduction renovation should not be secured the safety of structure but established the quality of lift. And questionnaire asking expert's opinion also supplemented. As a result, the item for examination and the weight for evaluation are suggested in functional deterioration. The results of this study are summarized as follows: 1. Existent redevelopment criteria are not appropriate to decide the environmental comfort, the vital function of housing and the residential management finance. 2. The future criteria have to evaluate the existing environmental comfort, be reconstructed to put first general deterioration. 3. Evaluation elements was extracted throughout the document research, evaluation elements analysis system was separated in residential area, suburb of dwellings, community, and housing complexity environmental assesment, 4. Each category of influence ratio was extracted to assess total deterioration throughout the expert question survey. 5. Each category analysed (1) $0{\sim}30%$: function is good (unnecessary to improve) (2) $31{\sim}40%$: average (necessary of partial improvement) (3) $41{\sim}59%$: bad (necessary of total improvement) (4) $60{\sim}100%$: the worst (unable to improve throughout the repair)

• 대한가정학회지
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• 제50권2호
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• pp.11-23
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• 2012

This qualitative study attempts to analyze the separation experiences of wild geese families from a social exchange perspective. The participants of this study were 16 mothers who lived apart from their partners for the purpose of educating their offspring. To collect data, in-depth interviews were conducted with the mothers about the decision-making process and the separation experiences. The results showed that there was a unique educational factor in Korea that affected wild geese families' decision on family separation. Geese families had to face psychological inconvenience caused by negative eyes from society. In addition, the fathers suffered loneliness and had difficulties in doing household chores, and the mothers experienced an overload of child parenting. On the other hand, emotional relationship between the husband and wife was enhanced, and the friendly relationship between a mother and her children was built. The biggest reward from the wild geese families turned out to be the educational achievement of their children.

• 건축역사연구
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• 제12권3호
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• pp.131-148
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• 2003

This study has main purpose to understand the traditional house, not by outside researcher's but by inside dweller's view. The house in Geochang, is descendent house of Jeong-On who was faithful scholar at Chosen Dynasty. In order to understand, I restore firstly the house form and space at traditional era, 60 years ago, and every dwellers not only family members but also servants and guests, by interviewing old matriarch. One of the main rules of the house disposition is Ancester worshipping life. Worshipping floor of Anchae, main building, is located unusually in front of Shrine for Jeong-On, in order to connect all activities directly. Sarangchae, men's building, opened to the funeral mourners, is separated by a wall because women have to serve foods and wail at the backside. Space for everyday life is separated by man/woman, insider/outsider, master/subordinate. Agricultural production is also key factor of the space allocation: big storage buildings for rice crop needed to tenant farming. Both activities of ancestor worship ceremony and guest greeting need large kitchen annex area for food preparation and serving space of rear veranda floor. A number of guests visit the house: guest-greeting activity is taken place from the family pavilion, Neungheo-Jeong and Saranchae by the social position. This study show that architectural space and form of the house reflects exactly dwellers life.