• Proceedings of the Korean Nuclear Society Conference
• /
• 1996.05b
• /
• pp.637-642
• /
• 1996

This paper presents the analysis results for the core degradation processes and the fission product release of the PHEBUS FPT0 experiment using MELCOR1.8.3. The objective of this study is to assess models associated with the core damage and fission product behavior in MELCOR. The calculation results were much improved through sensitivity studies. Thermal/hydraulic behavior in the core and the circuit was well predicted under the intact core geometry. In non-eutectic model case. the UO$_2$ dissolution model in the MELCOR always showed such a tendency that the resulting dissolved UO$_2$ mass was small at the highly oxidized condition due to the model logic. Total H$_2$ generation mass was underpredicted because the stiffner was not modeled and the liner in the shroud was not allowed to be oxidized in MELCOR. Some difficulties were found in modeling the activation product were solved by manipulating the RN input associated with the initial fission product inventory. These problem were occurred because there are no control rod model in MELCOR. Generally the fission product release ratio showed a similar trend compared with the measured data except the activation product. which have no model to simulate in MELCOR.

• Bulletin of the Society of Naval Architects of Korea
• /
• v.27 no.4
• /
• pp.72-82
• /
• 1990

A simple numerical analysis procedure has been proposed to trace the response of unstiffened offshore tubular members subjected to lateral impacts and eventually to estimate the consequential extent of damage. In the procedure a tubular member is reduced to a spring-mass system having two degrees-of-freedom. one for local denting deformation and the other for that of overall bending. Results of impact tests have been correlated with those of numerical analysis in order to achieve an empirical representation of the strain-rate sensitivity and other dynamic effects upon the spring coefficient for bending deformation. The theoretical estimates of extents of damage correlate reasonably well with those obtained in experiments.

• Plant Biotechnology Reports
• /
• v.2 no.1
• /
• pp.47-58
• /
• 2008

To select agronomically useful transgenic plants, a large number of transgenic events are initially produced, gene transfer confirmed, and advanced to obtain homozygous lines for testing in field trials. Direct in planta assays for identifying the transgene carriers in the segregating progeny are based on the activity of selectable marker gene and are easy, simple and inexpensive. For this purpose, expression of bar gene as measured by tolerance to damage by glufosinate ammonium, the active ingredient in the herbicide BASTA, was investigated. Dose damage curves were generated by leaf paint tests with BASTA on four genotypes of sorghum. Transgenic plants were characterized in terms of sensitivity to the concentration of glufosinate ammonium. In transgenics, symptoms of BASTA swab tests at different growth stages and PCR analysis for cry1B were carried out and correlated. Germination tests could not be employed for large scale evaluation of transgenic progeny because of mortality of tolerant seedlings after transplantation to soil. Based on the above findings, a simple, inexpensive, time-saving, two-step scheme for effective evaluation of transgenics and their progeny containing bar gene as selection marker using BASTA swab tests is described.

• Proceedings of the Zoological Society Korea Conference
• /
• 1998.10b
• /
• pp.113-113
• /
• 1998

In Saccharomyces cerevisiae UV irradiation and a variety of chemical DNA -damaging agents induce the transcription of specific genes, including several involved in DNA repair. One of the best characterized of DNA -damage inducible genes is PHRI, which encodes the apoenzyme for DNA photolyase. Basal-level and damage-induced expression of PHRI require an upstream activation sequence, UASPHRI. Here we report the identification of the UlvIE6 gene of S. cerevisiae as a regulator of UASPHRl activity. Surprisingly, the effect of deletion of UME6 is growth phase dependent. In wild-type cells PHRI is induced in late exponential phase, concomitant with the initiation of glycogen accumulation that precedes the diauxic shift. Deletion of UNIE6 abolishes this induction, decreases the steady-state concentration of photolyase molecules and PHRI mRNA, and increases the UV sensitivity of a rad2 mutant. The results suggest that UM E6 contributes to the regulated expression of a subset of damage-responsive genes in yeast. Furthermore, the upstream repression sequence, URSPHRI, is required for repression and damage-induced expression of PHRl. Here we show identification of YER169W and YDR096W as putative regulators acting through $URS_{PHRI}$. These open reading frames were designated as RPHI (YERl69W) and RPH2 (YDR096W) indicating regulator of PHRI. Simultaneous disruption of both genes showed a synergistic effect, producing a four-fold increase in basal level expression and a similar decrease m the induction ratio following treatment of methyl methanesulfonate(MMS). Mutation of the sequence ($AG_4$) bound by Rphlp rendered the promoter of PHRI insensitive to changes in RPHI or RPH2 status. The data suggest that RPHI and RPH2 act as damage-responsive negative regulators of PHRI. Surprisingly, the sequence bound by Rphlp in vitro is found to be $AG_4$ which is identical to the consensus binding site for the regulators Msn2p and Msn4p involved in stress-induced expression. Deletion of MSN2 and MSN4 has little effect on the induction$.$ ratio following DNA damage. However, all deletions led to a significant decrease in basal-level and induced expression of PHRI. These results imply that MSN2 and MSN4 are positive regulators of P HRI but are not required for DNA damage repression. [Supported by grant from NIH]om NIH]

• The Transactions of The Korean Institute of Electrical Engineers
• /
• v.65 no.5
• /
• pp.836-842
• /
• 2016

When an accident occurs in the nuclear power plant, the faulted information might mislead to the high possibility of aggravating the accident. At the Fukushima accident, the operators misunderstood that there was no core exposure despite in the processing of core damage, because the instrument information of the reactor water level was provided to the operators optimistically other than the actual situation. Thus, this misunderstanding actually caused to much confusions on the rapid countermeasure on the accident, and then resulted in multiplying the accident propagation. It is necessary to be equipped with the function that informs operators the status of instrument integrity in real time. If plant operators verify that the instruments are working properly during accident conditions, they are able to make a decision more safely. In this study, we have performed various tests for the fault detection sensitivity of an data-driven empirical model to review the usability of the model in the accident conditions. The test was performed by using simulation data from the compact nuclear simulator that is numerically simulated to PWR type nuclear power plant. As a result of the test, the proposed model has shown good performance for detecting the specified instrument faults during normal plant conditions. Although the instrument fault detection sensitivity during plant accident conditions is lower than that during normal condition, the data-drive empirical model can be detected an instrument fault during early stage of plant accidents.

• Structural Engineering and Mechanics
• /
• v.49 no.2
• /
• pp.203-223
• /
• 2014

The extensive use of prestressed reinforced concrete (PSC) highway bridges in marine environment drastically increases the sensitivity to both fatigue-and corrosion-induced damage of their critical structural components during their service lives. Within this scenario, an integrated method that is capable of evaluating the fatigue reliability, identifying a condition-based maintenance, and predicting the remaining service life of its critical components is therefore needed. To accomplish this goal, a procedure for fatigue reliability prediction of PSC highway bridges is proposed in the present study. Vehicle-bridge coupling vibration analysis is performed for obtaining the equivalent moment ranges of critical section of bridges under typical fatigue truck models. Three-dimensional nonlinear mathematical models of fatigue trucks are simplified as an eleven-degree-of-freedom system. Road surface roughness is simulated as zero-mean stationary Gaussian random processes using the trigonometric series method. The time-dependent stress-concentration factors of reinforcing bars and prestressing tendons are accounted for more accurate stress ranges determination. The limit state functions are constructed according to the Miner's linear damage rule, the time-dependent S-N curves of prestressing tendons and the site-specific stress cycle prediction. The effectiveness of the methodology framework is demonstrated to a T-type simple supported multi-girder bridge for fatigue reliability evaluation.

• Steel and Composite Structures
• /
• v.29 no.6
• /
• pp.703-716
• /
• 2018

Rapid detection of damages in civil engineering structures, in order to assess their possible disorders and as a result produce competent decision making, are crucial to ensure their health and ultimately enhance the level of public safety. In traditional intelligent health monitoring methods, the features are manually extracted depending on prior knowledge and diagnostic expertise. Inspired by the idea of unsupervised feature learning that uses artificial intelligence techniques to learn features from raw data, a two-stage learning method is proposed here for intelligent health monitoring of civil engineering structures. In the first stage, $Nystr{\ddot{o}}m$ method is used for automatic feature extraction from structural vibration signals. In the second stage, Moving Kernel Principal Component Analysis (MKPCA) is employed to classify the health conditions based on the extracted features. In this paper, KPCA has been implemented in a new form as Moving KPCA for effectively segmenting large data and for determining the changes, as data are continuously collected. Numerical results revealed that the proposed health monitoring system has a satisfactory performance for detecting the damage scenarios of a three-story frame aluminum structure. Furthermore, the enhanced version of KPCA methods exhibited a significant improvement in sensitivity, accuracy, and effectiveness over conventional methods.

• Structural Monitoring and Maintenance
• /
• v.6 no.2
• /
• pp.125-145
• /
• 2019

Bridge scour is one of the predominant causes of bridge failure. Current climate deterioration leads to increase of flooding frequency and severity and thus poses a higher risk of bridge scour failure than before. Recent studies have explored extensively the vibration-based scour monitoring technique by analyzing the structural modal properties before and after damage. However, the state-of-art of this area lacks a systematic approach with sufficient robustness and credibility for practical decision making. This paper attempts to develop a data-driven methodology for bridge scour monitoring using support vector machines. This study extracts features from the bridge dynamic responses based on a generic sensitivity study on the bridge's modal properties and selects the features that are significantly contributive to bridge scour detection. Results indicate that the proposed data-driven method can quantify the bridge scour damage with satisfactory accuracy for most cases. This paper provides an alternative methodology for bridge scour evaluation using the machine learning method. It has the potential to be practically applied for bridge safety assessment in case that scour happens.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
• /
• 2006.05a
• /
• pp.326-331
• /
• 2006

The durability of recent motors is longer than the past one because there are the rapid technique development of the automobile industry and the vehicle maintenance of users. And then the importance of the durability test due to vibration is increased from day to day. So full vehicle and parts companies accomplish the durability test using various methods. The most public test method among them is the reliable field test but it bring on higher cost and period of the development process. The durability test using MAST(multi axis simulation table) is a solution in order to improve the development process of automobiles. Generally its excitation source uses the optimized road profiles that are obtained by the road test of belgian road, country road, cobbleston road and so on instead of a real field but the interrelations and influences accordingly vehicle damage are considered by a field test between specific roads and real fields in the first place. Therefore this study, in order to accomplish a basic research for the durability test using the MAST, performed on the real field driving test at various domestic roads and the results which are analyzed by PSD(power spectrum density) are compared with relative sensitivity among the roads. Consequently they can present a basic material for generation of road profiles which is applied to the durability test using MAST.

• Environmental Mutagens and Carcinogens
• /
• v.9 no.1
• /
• pp.13-22
• /
• 1989

The effect of novobiocin (NOV), and inhibitor of topoisomerase II, on ethyl methanesulfonate (EMS)-or bleomycin (BLM)-induced DNA repair synthesis was examined during the cell cycle of Chinese hamster ovary (CHO)-K1 cells. Three assays were employed in this study: cell survival, alkaline elution and unscheduled DNA synthesis. EMS was effective at killing CHO cells in G1 phase, wheras BLM preferentially killed cells in G2 and S phases. EMS induced the much more amount of DNA damage in G1 phase, while BLM induced in G2 phase than the other phases. The both of pre- and post-treatment with BOV inhibitied EMS- or BLM-induced DNA repair synthesis in G1 and G2 phases, and pretreatment with NOV inhibited more effectively than the post-treated group. These results suggested that CHO cells exhibited a differential sensitivity to cell lethality and DNA damage in relation to cell cycle according to used chemical agents, and that DNA topoisomerase II participated in an initial stage of DNA repair.