Proceedings of the Zoological Society Korea Conference (한국동물학회:학술대회논문집)
- 1998.10b
- /
- Pages.113-113
- /
- 1998
Transcriptional Regulation of a DNA Repair Gene in Saccharomyces cerevisiae
- Jang, Yeon-Kyu (Department of Molecular Biology, Seoul National University) ;
- Sancar, Gwen-B. (Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill) ;
- Park, Sang-Dai (Department of Molecular Biology, Seoul National University)
- Published : 1998.10.01
Abstract
In Saccharomyces cerevisiae UV irradiation and a variety of chemical DNA -damaging agents induce the transcription of specific genes, including several involved in DNA repair. One of the best characterized of DNA -damage inducible genes is PHRI, which encodes the apoenzyme for DNA photolyase. Basal-level and damage-induced expression of PHRI require an upstream activation sequence, UASPHRI. Here we report the identification of the UlvIE6 gene of S. cerevisiae as a regulator of UASPHRl activity. Surprisingly, the effect of deletion of UME6 is growth phase dependent. In wild-type cells PHRI is induced in late exponential phase, concomitant with the initiation of glycogen accumulation that precedes the diauxic shift. Deletion of UNIE6 abolishes this induction, decreases the steady-state concentration of photolyase molecules and PHRI mRNA, and increases the UV sensitivity of a rad2 mutant. The results suggest that UM E6 contributes to the regulated expression of a subset of damage-responsive genes in yeast. Furthermore, the upstream repression sequence, URSPHRI, is required for repression and damage-induced expression of PHRl. Here we show identification of YER169W and YDR096W as putative regulators acting through
Keywords