• Asian Pacific Journal of Cancer Prevention
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• v.13 no.5
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• pp.2121-2127
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• 2012

Objective: Deoxyribonucleoside kinase of Drosophila melanogaster (Dm-dNK) mutants have been reported to exert suicide gene effects in combined gene/chemotherapy of cancer. Here, we aimed to further evaluate the capacity of the mutanted enzyme and its potential for inhibiting cancer cell growth. Methods: We altered the sequence of the last 10 amino acids of Dm-dNK to perform site-directed mutagenesis and constructed active site mutanted Dm-dNK (Dm-dNKmut), RT-PCR and western bloting studies were used to reveal the expression of lentivirus mediated Dm-dNKmut in a breast cancer cell line (Bcap37), a gastric cancer cell line (SGC7901) and a colorectal cancer cell line (CCL187). [3H]-labeled substrates were used for enzyme activity assays, cell cytotoxicity was assessed by MTT assays, cell proliferation using a hemocytometer and apoptosis induction by thenannexin-V-FITC labeled FACS method. In vivo, an animal study was set out in which BALB/C nude mice bearing tumors were treated with lentivirus mediated expression of Dm-dNKmut with the pyrimidine nucleoside analog brivudine (BVDU, (E)-5-(2-bromovinyl)-(2-deoxyuridine). Results: The Dm-dNKmut could be stably expressed in the cancer cell lines and retained its enzymatic activity. Moreover, the cells expressing Dm-dNKmut exhibited increased sensitivity in combination with BVDU, with induction of apoptosis in vitro and in vivo. Conclusion: These findings underlined the importance of BVDU phosphorylated by Dm-dNKmut in transduced cancer cells and the potential role of Dm-dNKmut as a suicide gene, thus providing the basis for future intensive research for cancer therapy.

• Journal of the Korean Magnetic Resonance Society
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• v.19 no.3
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• pp.106-111
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• 2015

Helicobacter pylori (H. pylori) survives in acidic and fluctuating pH conditions of the stomach. The pH effect on H. pylori proteins is important for the advanced understanding of its evolution and viability, although this bacterium has the molecular machinery that neutralizes the acidic condition. HP1492 is known as a conserved NifU-like protein from H. pylori. NifU is a nitrogen fixation protein that mediates the transfer of iron-sulfur (Fe-S) cluster to iron-sulfur proteins like ferredoxin. Commonly, the monomeric reduced state of NifU can be converted to the dimeric oxidized state by intermolecular disulfide bond formation. Because it remains unclear that HP1492 actually behaves as known NifU protein, we first found that this protein can adopt both oxidized and reduced forms using size exclusion chromatography. Circular dichroism experiment showed that HP1492 is relatively well-structured at pH 6.5, compared to other pH conditions. On the basis of the backbone resonance assignment of HP1492, we further characterized the residues that are sensitive to pH using NMR spectroscopy. These residues showing large chemical shift changes could be mapped onto the secondary structure of the protein. Our results could provide the foundation for structural and biophysical studies on a wide spectrum of NifU proteins.

• Journal of Navigation and Port Research
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• v.38 no.6
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• pp.623-627
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• 2014

In recent years, wireless communication technologies in human body have received an increasing attention and the research on an antenna that can be worn also has been actively conducted. In this paper, an wearable antenna that can receive GPS signal frequency is proposed. The antenna was manufactured by using a copper polyester fabric with thickness of 0.08mm as a radiator and a ground plate, and a goatskin with thickness of 0.7mm as dielectric substrate. Cutting edges placed in diagonal direction of square patch in order to obtain a circular polarization characteristic, and the conductive cloth and leather was laminated by using a conductive epoxy. First, goatskin dielectric constant was obtained through the simulation and measurement of resonance frequency of the three square patch antennas with different size. On the basis of the results, an antenna operating in the GPS band was designed and the performance of the antenna was validated by making the experiment. The change of the characteristic of the antenna that is located on the shoulder parts of the clothing and wearing person were measured. And it was confirmed that the reception sensitivity has a similar level as compared to the commercially produced ceramic GPS antenna.

• Journal of Advanced Marine Engineering and Technology
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• v.33 no.5
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• pp.672-678
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• 2009

Acoustic Emission (AE) technique is a non-destructive testing method and widely used for the early detection of faults in rotating machines in these days, because the sensitivity of AE transducers is higher than normal accelerometers. So it can detect low energy vibration signals. The faults in the rotating machines are generally occurred at bearings and gearboxes which are the principal parts of the machines. It was studied to detect the bearing faults by envelop analysis in several decade years. And the researches showed that AE had a possibility of the application in condition monitoring system(CMS) using the envelope analysis for the rolling bearing. And peak ratio (PR) was developed for expression of the bearing condition in condition monitoring system using AE. Noise level is needed to reduce to take exact PR value because the PR is calculated from total root mean square (RMS) and the harmonics peak levels of the defect frequencies of the bearing. Therefore, in this paper, the discrete wavelet transform (DWT) was added in the envelope analysis to reduce the noise level in the AE signals. And then, the PR was calculated and compared with general envelope analysis result and the result of envelope analysis added the DWT. In the experiment result about inner fault of bearing, defect frequency was difficult to find about only envelop analysis. But it's easy to find defect frequency after wavelet transform. Therefore, Envelop analysis added wavelet transform was useful method for early detection of default in signal process.

• Journal of KIISE:Computing Practices and Letters
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• v.9 no.5
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• pp.521-529
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• 2003

In this paper we present an improved method by using demographic information for overcoming the similarity miss-calculation from the sparsity problem in collaborative filtering recommendation systems. The similarity between a pair of users is only determined by the ratings given to co-rated items, so items that have not been rated by both users are ignored. To solve this problem, we add virtual neighbor's rating using demographic information of neighbors for improving prediction accuracy. It is one kind of extentions of traditional collaborative filtering methods using the peason correlation coefficient. We used the Grouplens movie rating data in experiment and we have compared the proposed method with the collaborative filtering methods by the mean absolute error and receive operating characteristic values. The results show that the proposed method is more efficient than the collaborative filtering methods using the pearson correlation coefficient about 9% in MAE and 13% in sensitivity of ROC.

• Journal of KIISE
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• v.44 no.7
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• pp.692-702
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• 2017

Face detection using the LBP based feature descriptor has issues in that it can not represent spatial information between facial shape and facial components such as eyes, nose and mouth. To address these issues, in previous research, a facial image was divided into a number of square sub-regions. However, since the sub-regions are divided into different numbers and sizes, the division criteria of the sub-region suitable for the database used in the experiment is ambiguous, the dimension of the LBP histogram increases in proportion to the number of sub-regions and as the number of sub-regions increases, the sensitivity to facial orientation rotation increases significantly. In this paper, we present a novel facial region segmentation method that can solve in-plane rotation issues associated with LBP based feature descriptors and the number of dimensions of feature descriptors. As a result, the proposed method showed detection accuracy of 99.0278% from a single facial image rotated in orientation.

• Atmosphere
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• v.30 no.1
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• pp.17-30
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• 2020

KMA performed the special observation program to provide information about severe weather and to monitor typhoon PRAPIROON using the ship which called the Gisang 1 from 29 June 2018 to 4 July 2018 (UTC). For this period, upper-air was observed 21 times with 6 hour intervals using rawinsonde in the Gisang 1. We investigated the impact of upper-air observation data from the Gisang 1 on the performance of the operational convective scale model (we called LDAPS). We conducted two experiments that used all observation data including upper-air observation data from the Gisang 1 (OPER) and without it (EXPR). For a typhoon PRAPIROON case, track forecast error of OPER was lower than EXPR until forecast 24 hours. The intensity forecast error of OPER for minimum sea level pressure was lower than EXPR until forecast 12 hours. The intensity forecast error of OPER for maximum wind speed was mostly lower than EXPR until forecast 30 hours. OPER showed good performance for typhoon forecast compared with EXPR at the early lead time. Two precipitation cases occurred in the south of the Korean peninsula due to the impact of Changma on 1 July and typhoon on 3 July. The location of main precipitation band predicted from OPER was closer to observations. As assimilating upper-air data observed in the Gisang 1 to model, it showed positive results in typhoon and precipitation cases.

• Korean Journal of Digital Imaging in Medicine
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• v.12 no.2
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• pp.81-87
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• 2010

The setting parameters of ultrasound scanner give influences to change of image. Sonographers have used a Matlab program to make Low Contrast Sensitivity(LCS) value and compared original images in order to evaluate the use of the supersonic diagnosis machinery. We confirmed the change of image in Grayscale values using Photoshop program. Experiment equipment of our research used A Medison Accuvix V10, A Multi-Tissue Ultrasound Phantom(040 GSE) of CHRIS Company, A Adobe Photoshop CS4 Program, A Convex Probe, A USB memory stick, A Probe Fixation Equipment. The method used Gain, Dynamic Range(DR) of the setting parameters of ultrasound scanner and researched Gain and DR was set to 10 dB. We changed the different settings to see the changes of images using Grayscale values of a Photoshop program about tissue images of a phantom. This study evaluated DR and Gain whether it is an image controller to get the optimum contrast to produce an image to see the how effect on the images. We did not use Gateway in supersonic diagnosis machinery. We can easily open to open the files through Photoshop program before we get Digital Imaging and Communications in Medicine(DICOM) files use USB memory stick in supersonic diagnosis machinery. When we diagnosed the lesion of the patient with ultrasound, the contrast and the Gray scale value of image are very important. In this research, we determined the optimum setting parameters that provided useful information to diagnose disease and evaluated the change of improved images.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.34 no.4
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• pp.391-402
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• 2016

Among the various remote sensing sensors compared to the electro-optical sensors, SAR (Synthetic Aperture Radar) is very suitable for assessing damaged areas induced by disaster events owing to its all-weather day and night acquisition capability and sensitivity to geometric variables. The conventional CD (Change Detection) method that uses two-date data is typically used for mapping damage over extensive areas in a short time, but because data from only two dates are used, the information used in the conventional CD is limited. In this paper, we propose a novel CD method that is extended to use data consisting of two pre-disaster SAR data and one post-disaster SAR data. The proposed CD method detects changes by using a similarity weight image derived from the neighborhood information of a pixel in the data from the three dates. We conducted an experiment using three single polarization ALOS PALSAR (Advanced Land Observing Satellite/Phased Array Type L-Band) data collected over Miyagi, Japan which was seriously damaged by the 2011 east Japan tsunami. The results demonstrated that the mapping accuracy for damaged areas can be improved by about 26% with an increase of the g-mean compared to the conventional CD method. These improved results prove the performance of our proposed CD method and show that the proposed CD method is more suitable than the conventional CD method for detecting damaged areas induced by disaster.

• Journal of Veterinary Clinics
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• v.32 no.2
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• pp.154-157
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• 2015

A major cause of diarrhea in a dog is an infection with bacteria which include Salmonella spp., Campylobacter (C.) spp., and Clostridium (Cl.) spp.. It is fastidious to identify these bacteria by the culture. The purpose of this experiment is to devise the method for detecting Cl. perfringens, C. jejuni, C. coli, and Salmonella spp. with rapid and high sensitivity. The fecal samples collected from 71 normal and 66 diarrheic dog feces were used to compare the prevalence of the enteric pathogens and to develop a multiplex real-time polymerase chain reaction (PCR) assay for clinical use. Detection of Cl. perfringens, C. coli, and C. jejuni in diarrhea feces was higher than normal feces. A developed multiplex real-time PCR is useful for determining the presence and quantity of pathogen-specific or other unique sequences with in a fecal sample.