Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Development of a Panel of Multiplex Real-Time Polymerase Chain Reaction Assays for Simultaneous Detection of Canine Enteric Bacterial Pathogens

개의 장내 병원균의 동시 검출을 위한 다중 실시간 중합효소연쇄반응분석 패널개발

  • Jang, Hye-Jin (Veterinary Laboratory Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Han, Jae-Ik (Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University) ;
  • Kang, Hyo-Min (Veterinary Laboratory Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Na, Ki-Jeong (Veterinary Laboratory Medicine, College of Veterinary Medicine, Chungbuk National University)
  • Accepted : 2015.04.23
  • Published : 2015.04.30
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Abstract

A major cause of diarrhea in a dog is an infection with bacteria which include Salmonella spp., Campylobacter (C.) spp., and Clostridium (Cl.) spp.. It is fastidious to identify these bacteria by the culture. The purpose of this experiment is to devise the method for detecting Cl. perfringens, C. jejuni, C. coli, and Salmonella spp. with rapid and high sensitivity. The fecal samples collected from 71 normal and 66 diarrheic dog feces were used to compare the prevalence of the enteric pathogens and to develop a multiplex real-time polymerase chain reaction (PCR) assay for clinical use. Detection of Cl. perfringens, C. coli, and C. jejuni in diarrhea feces was higher than normal feces. A developed multiplex real-time PCR is useful for determining the presence and quantity of pathogen-specific or other unique sequences with in a fecal sample.

개에서 설사를 일으키는 주요 원인이 되는 장내 병원균으로 Salmonella spp., Shigella spp., Campylobacter spp., Clostridium spp.가 있다. 이들 세균은 배양으로 검출이 어렵다. 본 실험에서는 Salmonella spp., C. coli, C. jejuni, 그리고 Cl. perfringens를 신속하고 민감하게 검출할 수 있는 방법을 고안하였다. 정상견 71마리와 설사증상이 있는 66마리에서 수집한 분변 시료에서 장내 병원균의 유병률을 알아보고자 하였다. 장내 병원균은 실시간 중합효소 연쇄 반응 분석을 이용하여 검출하였다. 설사변에서 Salmonella spp., C. coli, C. jejuni, Cl. Perfringens는 정상변보다 검출률이 높았다. 개발한 다중실시간 중합효소연쇄반응은 분변시료의 병원균 존재 및 양 또는 기타 고유 서열을 확인하는데 유용하였다.

Keywords

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