• 제목/요약/키워드: selective media

검색결과 333건 처리시간 0.019초

다양한 식품에서 Campylobacter jejuni 검출을 위한 real-time PCR과 배지배양법의 비교검증 (Comparison of Real-Time PCR and Culture Methods for Detection of Campylobacter jejuni in Various Foods)

  • 천정환;현지연;황인균;곽효선;한정아;김무상;김종현;송광영;서건호
    • 한국식품과학회지
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    • 제43권1호
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    • pp.119-123
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    • 2011
  • 본 연구에서는 두 종류의 선택배지를 활용한 배지배양법과 realtime PCR의 C. jejuni 검출능력을 비교하였다. 소시지, 쇠고기 분쇄육, 무순에 C. jejuni를 접종하고 Hunt broth로 증균배양 하였으며, mCCD agar와 Preston agar에 배양액을 획선도말하여 미호기적으로 배양하였다. 동시에 증균배양액에서 1 mL을 채취하여 realtime PCR을 실시하였다. 실험결과, real-time PCR은 쇠고기 분쇄육과 소세지에서 두 가지 선택배지와 비교하여 동일한 검출력을 보였으나 무순에서는 훨씬 더 많은 양성을 검출하였다(p<0.05). 두 배지간의 비교에서는 Preston agar와 mCCD agar는 통계학적 유의차가 없는 민감도를 보였다(p>0.05). 결론적으로 real-time PCR은 표준검출법인 배지배양법과 비교하여 동등하거나 우수한 민감도를 지닌 신속검출기법인 것으로 사료되며, 배지배양법에 앞서 선별검사로 사용할 경우 시간, 비용, 노동력 절감에 있어서 매우 유효한 방법이 될 것으로 판단된다.

Spectrophotometric Determination of Nitrite Based on Its Reaction with p-Nitroaniline in the Presence of Diphenylamine in Micellar Media

  • Afkhami, Abbas;Masahi, Shokofeh;Bahram, Morteza
    • Bulletin of the Korean Chemical Society
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    • 제25권7호
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    • pp.1009-1011
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    • 2004
  • In the present work a very simple, sensitive and selective spectrophotometric method for the determination of nitrite in micellar media is described. The method is based on the color reaction of nitrite with p-nitroaniline in the presence of diphenylamine in acid media. In order to remove the extraction step, Triton X-100, a non-ionic surfactant was used as micellar media. The optimum reaction conditions such as acid concentration, reagents concentration and effect of time have been studied and the analytical characteristics of the method such as limit of detection, linear range and molar absorptivity have been obtained. The interference of some anions and cations was also tested. The method was applied to the determination of nitrite in real samples.

다수 배지의 사용에 따른 방선균의 중복 분리빈도 (Reisolation Frequency of Soil Actinomycetes on Multiple Isolation Media)

  • 김창진;이강현;아끼라 시마즈;유익동
    • 한국미생물·생명공학회지
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    • 제22권3호
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    • pp.329-331
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    • 1994
  • For effective screening research, to isolate many different strains not duplicated would be essential. Three kinds of media were used for selective isolation of actinomycetes to test the reisolation frequency. Sixty species were isolated on Bennet's agar, 47 were on glycerol-asparagine agar and 77 were on humic acid-vitamin agar from 10 domestic soil samples. All of these isolates were identified to the genus level based on morphological characters and examined the sameness on each medium. The reisolation frequency between two different media was 10% or so(8.3~16.7%) and among three different media was 25% or so(22.1~27.7%).

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An Improved Selective Isolation of Rare Actinomycetes from Forest Soil

  • Seong, Chi-Nam;Park, Ji-Heok;Baik, Keun-Shik
    • Journal of Microbiology
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    • 제39권1호
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    • pp.17-23
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    • 2001
  • Various pretreatment procedures and selective media were applied to assess the optimal conditions for the isolation of rare actinomycetes from soil. Pretreatment of wet-heating for 15 min at 70$^{\circ}C$ and phenol treatment of soil suspension were the most effective methods for the isolation of these microorganisms. Hair hydrolysate vitamin agar (HHVA) was the most suitable medium for the recovery of rare actinomycetes. Thirty-five rare actinomycete strains were chosen using selective isolation approaches, then morphological and chemical properties of the isolates were determined. The isolates belonged to one of the following genus, Micromonospora, Microbispora, Actinoplanes and Streptosporangium.

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Zymomonas mobilis Plasmid Vector의 숙주세포 내에서의 안정성에 관한 연구 (Stabilities of Plasmid Vectors in Zymomonas mobilis)

  • 이상기;박은숙;황덕주;박무영
    • 한국미생물·생명공학회지
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    • 제15권5호
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    • pp.328-333
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    • 1987
  • 고효율 에타놀 생성 균주인 Zymomonas mobilis의 plasmid vector가 숙주세포 내에서 안정하게 유지되는지의 여부를 회분 및 연속배양 방법을 통하여 조사하였다. Z. mobilis 숙주세포는 전이된 plasmid의 크기와 배지의 조성에 따라 증식속도에 영향을 받음을 알 수 있었고 작은 크기의 plasmid를 비 선택성 (non-selective) 배지에서 배양한 경우 더 빠른 성장을 보였다. 또한 사용한 4종류의 plasmid vector 모두 숙주세포 내에서 30세대 이상에 걸쳐 93% 이상의 높은 안정성을 나타내면서 독립적으로 증식함이 확인되었다.

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A Novel Selective Frame Discard Method for 3D Video over IP Networks

  • Chung, Young-Uk
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • 제4권6호
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    • pp.1209-1221
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    • 2010
  • Three dimensional (3D) video is expected to be an important application for broadcast and IP streaming services. One of the main limitations for the transmission of 3D video over IP networks is network bandwidth mismatch due to the large size of 3D data, which causes fatal decoding errors and mosaic-like damage. This paper presents a novel selective frame discard method to address the problem. The main idea of the proposed method is the symmetrical discard of the two dimensional (2D) video frame and the depth map frame. Also, the frames to be discarded are selected after additional consideration of the playback deadline, the network bandwidth, and the inter-frame dependency relationship within a group of pictures (GOP). It enables the efficient utilization of the network bandwidth and high quality 3D IPTV service. The simulation results demonstrate that the proposed method enhances the media quality of 3D video streaming even in the case of bad network conditions.

페이딩 매질에서 대역확산 주파수도약 통신시스템의 전송특성 (Transmission Characteristics of FFH-SS Communication System in Fading Media)

  • 김원후;전계석
    • 한국음향학회지
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    • 제6권1호
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    • pp.5-14
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    • 1987
  • 이 논문은 시간 및 주파수 선택성페이딩이 동시에 존재하는 채널에서 대역확산 주파수 도약통신시스템의 전송특성을 제시한다. 수신기는 승적합산기를 포함한 binary noncoherent matched-filter fast FH-SS시스템이다. 이 시스템에서 SNR, 채널 선택도, 전력비 등의 변동에 따른 오차확률은 데이터율을 고려하지 않고 다이버시티 개념을 사용하여 구하였으며, 오차확율에 대한 데이터를 분석한 결과 FH-SS 시스템은 시간 선택성 페이딩 채널에서 전송특성이 우수함을 나타내었다.

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Online Selective-Sample Learning of Hidden Markov Models for Sequence Classification

  • Kim, Minyoung
    • International Journal of Fuzzy Logic and Intelligent Systems
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    • 제15권3호
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    • pp.145-152
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    • 2015
  • We consider an online selective-sample learning problem for sequence classification, where the goal is to learn a predictive model using a stream of data samples whose class labels can be selectively queried by the algorithm. Given that there is a limit to the total number of queries permitted, the key issue is choosing the most informative and salient samples for their class labels to be queried. Recently, several aggressive selective-sample algorithms have been proposed under a linear model for static (non-sequential) binary classification. We extend the idea to hidden Markov models for multi-class sequence classification by introducing reasonable measures for the novelty and prediction confidence of the incoming sample with respect to the current model, on which the query decision is based. For several sequence classification datasets/tasks in online learning setups, we demonstrate the effectiveness of the proposed approach.

Comparative Study of Virtual Methods Used for Library Service Delivery among Librarians in Southern Nigeria Private Universities

  • Titilayo Comfort Ilesanmi
    • International Journal of Knowledge Content Development & Technology
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    • 제14권1호
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    • pp.33-52
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    • 2024
  • This study examined the virtual methods of service delivery being deployed by librarians in private universities in Nigeria. The objective of the study was to analyse the virtual methods of service delivery among librarians and determine the predominant method used among the librarians. Survey research design was adopted for the study. Total enumeration technique was adopted for the study. A population of 181 librarians from 45 private universities in Southern Nigeria were involved. Data were gathered, analysed and presented using frequency counts, percentages, mean and standard deviation. The study found that librarians used different virtual methods of service delivery. Library website 157 (90%) and e-mail 132 (76.3%) were the prominent electronic media used by librarians while Facebook 59 (34%) and Whatsapp 57 (32.9%) were the predominant social media platforms applied for service delivery by librarians. Electronic media were more used for latest publications arrival, as well as library education and document delivery services while social media were more used for reference services and selective dissemination of information. In conclusion, virtual methods of service delivery were deployed by librarians in private universities for service delivery. In comparing the virtual methods, librarians used more of electronic media than social media to deliver services to their users. Therefore, electronic media were predominantly used than social media, many of the virtual methods were found to be underutilised while recommendations were proffered.

Analyzing Public Opinion with Social Media Data during Election Periods: A Selective Literature Review

  • Kwak, Jin-ah;Cho, Sung Kyum
    • Asian Journal for Public Opinion Research
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    • 제5권4호
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    • pp.285-301
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    • 2018
  • There have been many studies that applied a data-driven analysis method to social media data, and some have even argued that this method can replace traditional polls. However, some other studies show contradictory results. There seems to be no consensus as to the methodology of data collection and analysis. But as social media-based election research continues and the data collection and analysis methodology keep developing, we need to review the key points of the controversy and to identify ways to go forward. Although some previous studies have reviewed the strengths and weaknesses of the social media-based election studies, they focused on predictive performance and did not adequately address other studies that utilized social media to address other issues related with public opinion during elections, such as public agenda or information diffusion. This paper tries to find out what information we can get by utilizing social media data and what limitations social media data has. Also, we review the various attempts to overcome these limitations. Finally, we suggest how we can best utilize social media data in understanding public opinion during elections.