• Title/Summary/Keyword: selective detection

Search Result 582, Processing Time 0.023 seconds

Novel Detection Protocol for Erwinia amylovora in Orchard Soil after Removal of Infected Trees

  • Sujin Song;Byeori Kim;Kwang-Pyo Kim;Eunjung Roh
    • The Plant Pathology Journal
    • /
    • v.40 no.3
    • /
    • pp.282-289
    • /
    • 2024
  • Fire blight is a bacterial disease caused by Erwinia amylovora. In Korea, fire blight was first reported in 2015 in an orchard. If the infection is confirmed, all trees in the orchard must be removed and the orchard must remain closed for 3 years. Since 2020, if the number of trees infected with fire blight is less than 5% of the total trees in the orchard, only the infected tree and adjacent trees are removed in Korea. Three years after removal, the trees can be replanted after confirming that the orchard soil is free from E. amylovora. In this study, a protocol was established for detecting E. amylovora in soil via selective enrichment, using tryptic soy broth with 0.05% bile salts and 50 ㎍/ml cycloheximide, and real-time polymerase chain reaction. This protocol resulted in a 1,000-times improved detection limit for E. amylovora in soil samples compared to that in unenriched samples. Soil monitoring was performed for orchards where fire blight-infected trees had been removed 3-27 months prior; the selected orchards were monitored every 3 months. Monitoring confirmed that E. amylovora was not present in the soil at any site in any of the orchards. A new detection protocol facilitates the monitoring of E. amylovora in soil and could help permit the replanting of trees in orchards. Also monitoring results provide evidence that trees can be planted earlier.

Comparison of Real-Time PCR and Culture Methods for Detection of Campylobacter jejuni in Various Foods (다양한 식품에서 Campylobacter jejuni 검출을 위한 real-time PCR과 배지배양법의 비교검증)

  • Chon, Jung-Whan;Hyeon, Ji-Yeon;Hwang, In-Gyun;Kwak, Hyo-Sun;Han, Jeong-A;Kim, Moo-Sang;Kim, Jong-Hyun;Song, Kwang-Young;Seo, Kun-Ho
    • Korean Journal of Food Science and Technology
    • /
    • v.43 no.1
    • /
    • pp.119-123
    • /
    • 2011
  • In this study, performances of culture methods using two selective media and real-time PCR were evaluated for detection of Campylobacter jejuni (C. jejuni) in various food samples. Sausage, ground beef, and radish sprouts inoculated with C. jejuni were enriched in Hunt broth and then streaked onto modified cefoperazone charcoal deoxycholate agar and Preston agar, followed by incubation under microaerobic conditions. The enriched Hunt broth (1 mL) was used in real-time PCR assay. No statistical differences were observed in sensitivity among the two selective media and real-time PCR for sausage and ground beef. However, the number of positives by real-time PCR in radish sprouts was much higher than the two selective media (p<0.05). It appears that real-time PCR could be used as an effective screening tool to detect C. jejuni, particularly in foods with a high number of background microflora such as fresh vegetables.

A Robust Continuous Object Tracking Protocol Using Chained Selective Wakeup Strategy in Wireless Sensor Networks (무선 센서 네트워크에서 연결된 선택적 활성화 기법을 사용하는 강건한 연속 객체 추적 프로토콜)

  • Hong, Hyungseop;Kim, Sang-Ha
    • The Journal of Korean Institute of Communications and Information Sciences
    • /
    • v.38B no.1
    • /
    • pp.72-79
    • /
    • 2013
  • In wireless sensor networks, the selective wakeup scheme is one of the energy saving mechanisms, that is used for an object detecting or tracking. Recently, many protocols are proposed using the selective wakeup scheme for the continuous objects tracking such as forest fires and poison gas. They predict the future shape of continuous objects and activate only sensors in the predicted boundary area of the objects. It works correctly in a uniformly deployed wireless sensor networks. However, it cannot be directly applied to a randomly deployed sensor networks with voids. When the predicted area is in the void area, the activation message cannot reach and the predicted area cannot be activated at the right time. It leads to many detection errors for continuous object. Moreover, if a sensor is once foiled in a activation control then the next activation control might be continuously failed. The detection errors can be result in serious harm to people. In this paper, we propose a chaining selective wakeup scheme for robust continuous object tracking in wireless sensor networks. In our protocol, we collect the information of a void area during the network configuration time; if the next boundary area is in the void area, we activate the chained area surrounding the void area with activation control message.

A Selective Attention Based Target Detection System in Noisy Images (잡영 영상에서의 선택적 주의 기반 목표물 탐지 시스템)

  • 최경주;이일병
    • Proceedings of the Korean Information Science Society Conference
    • /
    • 2002.04b
    • /
    • pp.622-624
    • /
    • 2002
  • 본 논문에서는 선택적 주의에 기반한 잡영 영상에서의 목표물 탐지 방법에 대해 기술한다. 특히 제안하는 방법은 목표물에 대한 아무런 지식을 사용하지 않고, 단지 입력되는 영상의 상향식 단서만을 사용하여 목표물을 탐지해냄으로써 여러 다양한 분야에 일반적으로 사용될 수 있다. 제안하는 시스템에서는 몇 가지 기본 특징들이 입력된 영상에서 바로 추출되며, 이러한 특징들이 서로 통합되어 가는 과정에서 목표물 탐지에 유용하지 않은 정보는 자연스럽게 걸러지며, 유용한 정보는 추가되고 부각되어진다. 간단한 영상부터 복잡한 자연영상에 이르는 다양한 잡영 영상을 대상으로 실험하여 제안하는 시스템의 성능을 평가하였다.

  • PDF

A Study on TCM for Digital Land Mobile Communication (디지탈 육상이동통신을 위한 TCM에 관한 연구)

  • 방성일;진연강
    • Journal of the Korean Institute of Telematics and Electronics A
    • /
    • v.31A no.2
    • /
    • pp.1-8
    • /
    • 1994
  • In this paper, a model of trellis-coded M-ary PSK (TCM/M-PSK) for digital land mobile communication is realized. Bit error rate(BER) of TCM/M-PSK is calculated theoretically in time selective fading environment due to Doppler spread in the presence of AWGN. cochannel interference. These analyses are employed the receiver with post detection diversity and the direct sequence to improve the BER performance of TCM-MPSK system. It is shown that the CNR required by the diversity reception and the direct sequece(process gain=20dB) TCM-4PSK system is reduced to about 25 dB.

  • PDF

A Study on Threshold-based Denoising by UDWT (UDWT을 이용한 경계법에 기초한 노이즈 제거에 관한 연구)

  • 배상범;김남호;류지구
    • Proceedings of the Korea Institute of Convergence Signal Processing
    • /
    • 2001.06a
    • /
    • pp.77-80
    • /
    • 2001
  • This paper presents a new threshold-based denoising method by using undecimated discrete wavelet transform (UDWT). It proved excellency of the UDWT compared with orthogonal wavelet transform (OWT), spatia1ly selective noise filtration (SSNF) and NSSNF added new parameter. Methods using the spatial correlation are effectual at edge detection and image enhancement, whereas algorithm is complex and needs more computation However, UDWT is effective at denoising and needs less computation and simple algorithm.

  • PDF

SACK TCP with Probing Device

  • Liang, Bing;Hong, Choong Seon
    • Annual Conference of KIPS
    • /
    • 2004.05a
    • /
    • pp.1355-1358
    • /
    • 2004
  • This paper describes a modification to the SACK (Selective Acknowledgement) Transmission Control Protocol's (TCP), called SACK TCP with Probing Device, SACK works in conjunction with Probing Device, for improving SACK TCP performance when more than half a window of data lost that is typical in handoff as well as unreliable media. It shows that by slightly modifying the congestion control mechanism of the SACK TCP, it can be made to better performance to multiple packets lost from one window of data.

  • PDF

Gas Chromatography/Mass Spectrometry and Gas Chromatography/Tandem Mass Spectrometry of some s-Triazine Pesticides

  • Kim, Yoo-Joong;Kim, Myung-Soo
    • Bulletin of the Korean Chemical Society
    • /
    • v.10 no.2
    • /
    • pp.196-200
    • /
    • 1989
  • Some s-triazine herbicides, namely simazine, atrazine, and propazine present as trace components in a complex mixture were analyzed by GC/MS and GC/MS/MS methods. Even though monitoring the molecular ions was the best in terms of sensitivity, adequate analysis could not be done when interfering species were present. When doubly charged ions which appeared at characteristic m/z values were monitored, chromatograms were rather free from interference. More importantly, selected reaction monitoring was found to provide a selective means of detection with general applicability.

A Study of the characteristics of NOx measurement and analysis methods of the SCR system for ships (선박용 SCR 시스템 NOx 측정 및 분석 방식의 특성 연구)

  • Kim, Sung-Yoon;Lee, Young-Ho;Kim, Min;Park, Sam-Sik
    • Journal of Advanced Marine Engineering and Technology
    • /
    • v.39 no.4
    • /
    • pp.387-392
    • /
    • 2015
  • A method for measuring and analyzing the $NO_x$ in ships is described in $NO_x$ Technical Code 2008. The analysis device, as required by the Code, has been to use a Chemi-luminescence detection method or Heated Chemi-luminescence detection. on the other hand, selective catalytic reduction using $NH_3$ as a reducing agent has an interference effect on the analyzer, and causes measurement error. In this study, the Chemi-luminescence detection method was examined according to how it affects the concentration of $O_2$, CO, $SO_2$, $NH_3$. Fourier transform infrared spectrometry analysis equipment and measurement methods were compared. In order to confirm the effect of the physical interference of the measuring device, it was confirmed by decomposing a measuring device. Consequently, white precipitate and moisture were generated inside the chemiluminescence detection system and I found that affecting interference. The influence of interference highlights the need to consider the minimized $NO_x$ measurement method.