Gourd seeds were irradiated with the doses of 0-20 Gy to investigate the effect of the low dose $\gamma$-radiation on the early growth and physiological activity. The stimulating effects of the low dose y- radiation on the early growth were not noticeably high, but were increased generally at 4-16 Gy irradiation group. The catalase and peroxidase activity of cotyledon from seeds irradiated with $\gamma$- radiation were increased at 8 Gy irradiation group. The photochemical activity of leaf was noticeably high at 4 Gy irradiation group. The photochemical yield of PSII, estimated as Fv/Fm, decreased with increasing illumination time by 50% after 4 hrs in the control and 8 Gy irradiation group, while Fo slightly increased. However, Fv/Em in the 4 Gy irradiation group decreased by 40% of inhibition, indicating that photoinhibition decreased by the low dose $\gamma$- radiation. Changes in the effective quantum yield of PSII, $\varphi_{PSII}$ and 1/Fo- l/Fm, a measure of the rate constant of excitation trapping by the PSII reaction center, showed similar pattern to Fv/Em. NPQ decreased by 70% after photoinhibitory treatment with showing similar pattern between the control and the irradiation group. These results showed the positive effect of low dose $\gamma$- radiation on the seedling growth and the reduction of photoinhibition in the 4 Gy irradiation group.
The effects of climatic factors on organisms lire variable and complex, and it, however, can be interpreted in terms of those on the distribution and those on the population densities. The distribution of an organism may largely be determined by the temperatures, except some temporal organisms which are depended on the air mass movements. Population density of an organism is determined by various climatic factors, such as previous winter temperature, temperature of growing season and rainfall. The start of growing season of the rice plants has been shifted to earlier since last decade in Korea. This may mean that the overall climatic condition during the growing season might be considerably different from those in past years, and such a difference in climatic conditions might have close relation with the recent status of the diseases and insect pests through direct effects on the physiology and population dynamics of the organisms, as well as through on the biotic associations of the pest organisms. The white back planthopper and brown planthopper have become the key insect pests in Korea in recent years. They are migratory and have high reproductive pontentials and more generations than average residential insects. The synchronization of the migrants and physiological condition of the rice plants seems to be the important factors in relation to the recent outbreaks of these insects; the high reproductive rate can be obtained with the growth stage of rice being 30-50 days after transplanting. The modication of the microclimate associated with high plant density and some other introduced new cultural techniques also have some relation with the outbreak. The key diseases of the rice are the blast disease, sheath blight and the bacterial leaf blight. For the rice blast, the seedling blast and leaf blast during the early growing season and the neck blast, have become more serious, the former may be related to hotbed nursery and the later may be related to the high humidity in early August, and synchronization of the heading time which has been shifted to early part from middle or late part of August. In general, for the rice diseases, the development of the new races have been the most serious which are largely resulted from the introduction of the new varieties, but it also seems to be related with the prolonged periods of the favorable condition associated with the shifted growing seasons. In general, the diseases and insect pest problems have become much more variable and complex, and control measures should be based on the thorough knowledge of the ecology of the pest organisms, that is, effects of various environmental factors on the disease cycle; spore release, spore deposition, infection, colonization and sporulation of the disease organisms, and those on the development, reproductive potentials, dispersal, age specific responses of the insects. The well organized real-time pest management systems, such as alfalfa weevil management system developed at the Purdue University in U.S., is the prime importance for the implementation of the pest management principles.
Horticultural traits and genetic relationship were evaluated for 83 melon (Cucumis melo L.) cultivars. Survey of a total of 36 characteristics for seedling, leaf, stem, flower, fruit, and seed and subsequent multiple analysis of variance (MANOVA) were conducted. Principal component analysis (PCA) showed that 8 principle components including fruit weight, fruit length, fruit diameter, cotyledon length, seed diameter, and seed length accounted for 76.3% of the total variance. Cluster analysis of the 83 melon cultivars using average linkage method resulted in 5 clusters at coefficient of 0.7. Cluster I consisted of cultivars with high values for fruit-related traits, Cluster II for soluble solid content, and Cluster V for high ripening rate. Genotyping of the 83 cultivars was conducted using 15 expressed-sequence tagged-simple sequence repeat (EST-SSR) from the Cucurbit Genomics Initiative (ICuGI) database. Analysis of genetic relatedness by UPGMA resulted in 6 clusters. Mantel test indicated that correlation between morphological and genetic distance was very low (r = -0.11).
To select a suitable indigenous plant for the phytoremediation of TNT contaminated soil, eight representative species of native grasses were tested to identify TNT toxicity thresholds. The threshold was determined based on various factors including cumulative seed germination, root and shoot length, fresh biomass, and the amount of water uptake under various TNT concentrations. Phytotoxic effect of TNT on plants was increased with the increase in TNT concentration but the degree was varied between grass species. Concentrations up to 60-80mgTNT/liter did not affect germination of Abutilion avicennae, Echinochioa crusgalli var. frumentacea, and Aeschynomene indica. Phytotoxicity threshold inhibition (50%) of Abutilion avicennae, schinochioa crusgalli var. frumentacea, Aeschynomene indica were 5-40mgTNT/liter for root length, 50-73mg TNT/Liter for shoot length and 68-99mgTNT/Liter for fresh biomass during 14 days of seedling exposure. Root and shoot growth as well as fresh biomass decreased as TNT concentration increased. In addition, the amount of water uptake decreased with increasing TNT concentration in Abutilion avicennae and Aeschynomene indica. Comparison of toxicity thresholds for the tested grass species showed that sensitivity of plants to TNT was in the order of root length > shoot length > fresh biomass > germination rate. From these results, we concluded that Abutilion avicennae and Aeschynomene indica had tolerance to TNT among the species tested.
Rearing condition and species of microalgae on growth and survival of the sunray surf clam, Mactra chinensis larvae were investigated for artificial seedling production. The larvae of M. chinensis on higher temperature showed high growth and low survival and was grown over $230{\mu}m$ in shell length 14 days after hatching, but low growth as $151.1{\mu}m$ at $18^{\circ}C$. The larvae of M. chinensis on salinity showed highest daily growth and survival as $11.3{\mu}m$ and 65.8% at 30 psu, respectively. The optimum of water temperature and salinity for the larval rearing were $23^{\circ}C$ and 30-35 psu over 25 psu at least. The density of larval rearing was below 10 per 1 ml in rearing seawater for elevating the development rate from D-shaped to settled (metamorphosing) stage. The larvae fed the mixed diet of Isochrysis galbana, Pavlova lutheri, Isochrysis sp. (green), Chlorella ellipsoidea showed highest growth and survival. The larvae fed the single diet of I. galbana, Isochrysis sp. (green) showed high growth and survival. But the larvae fed the single diet of P. lutheri and C. ellipsoidea showed low growth and survival. The optimum diet of larvae of M. chinensis was over two species of microalgae included I. galbana, Isochrysis sp. (green) for elevating the high growth and survival.
M1 plants which were produced from seed soaking in chemical mutagen, EMS or NaN$_3$, appeared wide morphorogical variations such as dwarf, albino, twisted leaf, white streaked leaf, and purpled stem. In mutants of reproductive organs, there were monoecious plants such as female-flower plant and male-flower plant, multiple spikes, and steriled plants among M1 plants. Also, barren stalk was increased significantly in M1 plants. Ear bagging at ear initiation stage prevented seed set on cob in normal plants. In spite of ear bagging, M1 plants which had cobs with seed set was 3.9-11.2% of stalks developed from seeds soaking with mutagens, but only three or four kernels could be matured on a cob. Ear bagging after mutagen injection into initiating ear produced 5.1-10% in cobs with seed set, but only 1.7-6.3 kernels could be matured. Cobs removed silk at four hours after artificial pollination increased the rate of cobs with seed set to 27%. Microscopic observation confirmed that ontogeny of kernels matured from ear bagging and mutagen treatment would be both adventitious and diplosporous apomictic reproduction. Chromosome set of M2 seedling was found to be diploid type in chromosomal counting of root tip. As M$_2$ plants showed an uniform appearence within each lines and their CV of plant height were ranged 4-6% in each lines, we concluded that they were apomictic progeny. But we could not find any marker traits combined with apomixis.
Journal of the Korean Society of Environmental Restoration Technology
/
v.20
no.6
/
pp.117-131
/
2017
This study was conducted to draw vegetation landscape elements in the ancient literature, investigate plant community structure, understand vegetation structure, and suggest reasonal conservation management methods. As a result of analyzing ancient literature, geomorphological landscapes in Guryong valley were canyon landscape and valley surrounded in Mt. Chiak. Plant landscape factors were as followed. Rhododendron spp. spread around valley. Also, Pinus densiflora communities were distributed in Guryong valley around. Especially, the entrance zones of Guryong valley were described as covered with Pinus densiflora and Whangchangkumpyo(黃腸禁標). Therefore, it was estimated that entry of Guryong valley was covered with Pinus densiflora community landscape. As for current vegetation result, the main vegetation was divided into mixed deciduous broad-leaved trees community and Pinus densiflora community. As a result of analysis by TWINSPAN for community classification, five communities(Deciduous broadleaved trees, Pinus densiflora, Pinus densiflora-Abies holophylla, Abies holophylla, and Pinus koraiensis community) were classified. To maintain historic plant landscape and conserve crucial resources, Pinus densiflora community was classified as concentrated conservation management area($105,472m^2$). To maintain Pinus densiflora landscape which has high historical and cultural value of Guryong valley, it was considered that active density control of lower layer vegetation would be necessary. Accordingly, to conserve P. densiflora landscape, Whangchangkumpyo(?腸禁標), that area was separated into Pinus densiflora lower layer forest management area($84,029m^2$) and Pinus densiflora seedling conservation management area($21,443m^2$). In understory of Pinus densiflora lower layer flora, the target tree species for elimination and management were Quercus serrata and Quercus mongolica. They were $4{\sim}6trees/100m^2$ and their average diameter was 7.1cm. To preserve Pinus densiflora seedlings, areas with Sasa borealis, the ground vegetation of Pinus densiflora community, rate of 80% or more should be selected as priority management areas and concentrated elimination and management of Sasa borealis should be implemented. Likewise, traditional Pinus densiflora forest is a historically cultural heritage to preserve with sustainable interest and survey. Efficient management method through systematic monitoring system should be made.
Kim, Young-Guk;Lee, Seoung-Tack;Chang, Young-Hee;Im, Dae-Joon;Yu, Hong-Seob;Kim, Choong-Guk
Korean Journal of Medicinal Crop Science
/
v.2
no.2
/
pp.105-109
/
1994
This experiment was conducted to know the labor saving effect and reducing production cost by agricultural mechanization in the cultivation of Bupleurum falcatum. Labor reducing effects of the drilling seeder by hand and the machine attached to two wheel tiller were 97%, but emergency rate was highest in the former. Dry root yield per plant was increased by low amount of seed sowing but that yield per unit area was increased at much seeding amount in the seeder attached to the tiller. The drilling seeder by hand was showed highest standing ratio of seedling and produced yield to 84.1kg of root yield per 10a. Labor saving effect was the best at the multipurposes mechanized harvester and labor saving and famer's income ratio were increased to 69% and 50% respectively. Labor time and cost were reduced to 74% and 69% respectively by mechanization of sowing and harvest cultivation practice on Bupleurum falcatum.
As seed production program is growing prosperously in various fishes in southern Korea, disease problems in larval and juvenile stages have emerged as a new research object. The following results were obtained from investigation about environmental factors related to mass mortalities of young Kinki red seabream, Pagrus major in process of artificial seedling production. Total length of red seabream larvae hatched was 2.93mm, and became 18.83~20.12mm at day 40. The first noticeable mortality of red seabream larvae (7.98~9.37mm) occurred in 25~30 day-old fish with the survival rates of 59.8~60.3%. Thereafter the mortality of larvae decreased, survival rate was 20.5~25.45% in day 40. After 20~30 days, the quality of pond water was bellow II class. During the experimental period COD, $PO_4$-P, $NO_2$-N, $NO_3$-N and $NH_$-N increased up to 3, 7, 34, 6 and 8 times, respectively, compared to initial ones. The number of viable bacteria in pond water and seabream larvae were $6.3{\times}10^6$~$2.3{\times}10^7$ cfu/ml, 4.3~$7.4{\times}10^6$ cfu/g in day 25, respectively. Among the isolated bacteria from the diseased red seabream in day 25, Vibrio spp. was considered to be the causative organism. External symptoms of this disease were floated, spined near the surface and inflated abdomen. When the isolated strain of the Vibrio was bathed to seabream larvae, $LD_50$ of seabream larvae was over $10^6$ cfu/ml of Vibrio spp.
Kim, Jae-Sung;Kim, Jin-Kyu;Back, Myung-Hwa;Kim, Dong-Hee
Journal of Radiation Protection and Research
/
v.24
no.3
/
pp.123-129
/
1999
Tomato (Lycopericum esculentum $M_{ILL}$ cv. Seokwang and cv. Housemomotaro) seeds were irradiated with the doses of $1{\sim}20$ Gy from $^{60}Co$$\gamma$-ray source to investigate the effect of the low dose $\gamma$-ray radiation on the early growth and resistance to subsequent high dose of radiation. Germination rate of seeds irradiated with low dose $\gamma$-ray was enhanced in Seokwang cultivar but not in Housemomotaro cultivar. Seedling height increased in 4 Gy and 8 Gy irradiation group of both cultivars. Plant height of Seokwang cultivar was depressed in low dose irradiation group but fresh weight was increased in 2 Gy and 4 Gy irradiation group. In Housemomotaro cultivar, plant height increased in 12 Gy and 20 Gy irradiation group and fresh weight increased in 4 Gy and 20 Gy irradiation group. Growth inhibition of tomato plants by high dose radiation was noticeably reduced by pre-irradiation of low dose radiation. Resistance to subsequent high dose of radiation was enhanced in 2 Gy and 8 Gy Irradiation group of Seokwang cultivar and in 2 Gy and 12 Gy irradiation group of Housemomotaro cultivar.
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