• The Plant Pathology Journal
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• 제24권1호
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• pp.31-35
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• 2008

The presence of Chrysanthemum stunt viroid (CSVd) in seed and pollen of diseased chrysanthemum was demonstrated. In seeds infected male parent from crosses in May, CSVd was transmitted to 6.7% of the progeny seedlings, whereas if the female parent was infected, CSVd transmission rate was between 46.9 and 75.7%. A relatively high incidence of 94.4 to 96.0% seed transmission occurred when both parents were infected. In seeds infected male parent from crosses in December, no progeny seedlings were infected with CSVd, whereas if the female parent did, CSVd transmission rate was 1.5%. When both parents were infected, 6.9% seed transmission was occurred. The seed transmission rate depended on the temperature when the crosses were made. CSVd was not detected in the non-infected female parent pollinated with infected pollen but was transmitted to the progenies. This is the first report of seed-borne transmission of CSVd in chrysanthemum.

• 식물병연구
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• 제19권2호
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• pp.114-117
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• 2013

To facilitate their spread, plant viruses have developed several methods for dispersal including insect and seed transmission. While insect transmission requires virus stability against insect digestion, seed-transmitted viruses have to overcome barriers to entry into embryos. Bean pod mottle virus (BPMV) is transmitted through seed at levels typically below 0.1%, but co-infection with Soybean mosaic virus (SMV) enhanced the seed transmission rate of BPMV in one experiment. In contrast, the rate of SMV seed transmission was not affected by BPMV co-infection. In a second preliminary study, the rate of SMV transmission was lower in an isoline of Williams 82 that contained a null mutation for the Kunitz trypsin inhibitor gene than in Williams 82. In this preliminary study, we observed that factors such as protease inhibitor expression and dual infection may affect the frequency of seed transmission of BPMV and SMV.

• The Plant Pathology Journal
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• 제30권2호
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• pp.178-182
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• 2014

Among imported plants, seeds are the items that have many latent pathogens and are difficult to inspect. Also, they are the import and export items whose market is expected to expand. The biggest problem with seeds is viruses. Prune dwarf virus (PDV) is the virus that is commonly inspected in Prunus cerasifera, P. persica, P. armeniaca, P. mandshurica, P. cerasus, P. avium or P. serotina seeds. In this study, two RT-PCR primer sets, which can promptly and specifically diagnose plant quarantine seed-transmitted PDV, were developed; and nested PCR primers, where products amplify 739 and 673 nucleotides (nt), and an nested PCR-product, 305 nt, can be obtained as these products are amplified again, were developed. Also, a modified-positive control plasmid was developed, where the restriction enzyme XhoI, which can identify the contamination of samples from the control, was inserted. The method developed in this study has detected PDV in 18 cases since 2007, and is expected to continuously contribute to the plant quarantine in Korea.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.144.2-145
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• 2003

This study was performed to investigate the incidence of Hosta uirus X (HVX), a Potexvirus, from cultivated hosta ornamental plants in Korea and to ascertain seed transmission of the virus from infected parent plant to progeny ones for breeding program of hosta plants. Infection rate of HVX in cultivated hostas was 25.6 ％ (11 out of 43 collected samples contained HVX) based on Western blot and RT-PCR detection methods. Most of HVX-infected hostas showed visible systemic leaf symptoms (mosaic, mottle, curling, stunting or combinations). Variability of HVX was confirmed by sequences of coat protein gene of individual isolates from different hostas. HVX was seed-transmitted on Hosta 'Blue Cadet'. The virus was detected from seeds, and sprouts and seedlings from the virus-contaminated seed sources. Over 7.5 ％ of seeds were HVX-contaminated surveyed in this study, Our data suggest that HVX can be transmitted by seed source, and indexing of the virus should be done for breeding program of Hosta.

• Mycobiology
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• 제30권1호
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• pp.47-50
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• 2002

Seed-borne fungi of some maize cultivars/lines grown during the months from May to September of 2001, collected from Dongguk University farm, Go Young City, IL Sang Gu, Korea were detected by blotter method. In all six fungi namely Alternaria alternata(Fr.) Keissler, Aspergillus niger Van Tiegh, Fusarium moniliforme Sheldon, Fusarium sp., Penicillium sp. and Ustilago zeae Unger. were found to associated with maize seeds. Prevalence of seed-borne fungi also varied. The highest percentages of seed-borne fungi were recorded with Fusarium moniliforme and the lowest in Penicillium sp. Transmission of all seed-borne pathogens from seeds to seedlings were also detected by test tube seedling symptom test. Among the seed-borne fungi, Alternaria alternata, Fusarium moniliforme and Fusarium sp. produced distinct seed rot and seedling infection symptoms. All the transmitted seed-borne fungi might be caused primary source of infection to the maize crop.

• Animal cells and systems
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• 제9권1호
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• pp.37-40
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• 2005

In okra Macrophomina phaseolina and Furasium verticilloides cause collar-rot, seedling-rot and other severe diseases at fruit maturing stages. These stages were located in all the components of the seeds. The seeds collected from seeds infected with Macrophomina phaseolina and Fusarium verticilloides revealed 100% infection. Such seeds resulted in pre- and post-emergence mortalities. Inoculated seeds also showed pre- and post-emergence death of the seedlings. The fungi seed-transmitted showed disease symptoms at different growth of okra plant. Fusarium verticilloides causes the wilt and Macrophomina phaseolina causes the collar-rot. Until now seed transmission of these fungi have not been studied. Hence, in the present study an attempt has been made to fill this lacunae.

• 대한전자공학회논문지TE
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• 제39권2호
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• pp.51-59
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• 2002

본 논문에서는 음성 통화에서의 안전한 전송과 수신을 위하여 SEED 알고리즘을 이용한 음성 암호 시스템 설계를 하였다. 음성영역의 신호는 CODEC에 의해 디지털 신호로 변환된다. 그리고 개선된 SEED 알고리즘을 적용한 DSP는 이 신호를 암호화한다. CODEC은 암호화된 신호를 아날로그 음성신호로 변환한다. 이 음성 신호는 중간에 도청이나 감청을 한다고 하더라도 암호화되어있기 때문에 안전하게 전송할 수 있다. 수신자는 수신된 음성신호를 복호화 SEED 알고리즘을 이용하여 송신자의 원음성을 들을 수 있다. 본 논문에서는 16라운드인 SEED 알고리즘의 라운드 수를 32라운드로 설계하여 truncated differential 확률을 $2^{-143.1}$에서 $2^{-286.6}$이상으로 개선하였다.

• Mycobiology
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• 제39권3호
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• pp.206-218
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• 2011

The seed-borne mycoflora of sorghum and foxtail millet collected from different growing areas in South Korea were isolated and taxonomically identified using dry inspection, standard blotter and the agar plate method. We investigated the in vitro and in vitro germination rates of disinfected and non-disinfected seeds of sorghum and foxtail millet using sterilized and unsterilized soil. The percent recovery of seed-borne mycoflora from the seed components of sorghum and foxtail millet seeds was determined and an infection experiment using the dominant species was evaluated for seedling emergence and mortality. A higher number of seed-borne fungi was observed in sorghum compared to that of foxtail millet. Eighteen fungal genera with 34 fungal species were identified from the seeds of sorghum and 13 genera with 22 species were identified from the seeds of foxtail millet. Five dominant species such as Alternaria alternata, Aspergillus flavus, Curvularia lunata, Fusarium moniliforme and Phoma sp. were recorded as seed-borne mycoflora in sorghum and 4 dominant species (Alternaria alternata, Aspergillus flavus, Curvularia lunata, Fusarium moniliforme) were observed in foxtail millet. The in vitro and in vitro germination rates were higher using disinfected seeds and sterilized soil. More seed-borne fungi were recovered from the pericarp compared to the endosperm and seed embryo. The percent recovery of seed-borne fungi ranged from 2.22% to 60.0%, and Alternaria alternata, Curvularia lunata and 4 species of Fusarium were isolated from the endosperm and embryo of sorghum and foxtail millet. Inoculation of the dominant seed-borne fungi showed considerable mortality of seedlings. All the transmitted seed-borne fungi might well be a primary source of infection of sorghum and foxtail millet crops.

• The Plant Pathology Journal
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• 제22권1호
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• pp.63-67
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• 2006

Apple scar skin, one of the most destructive diseases affecting apple, is caused by Apple scar skin viroid (ASSV d). Fruit dappling appeared on several cultivars in Korea and has been distributed to major cultivated areas since 2001. ASSVd was identified from infected fruits by using nucleic acid sequence-based amplification with electrochemiluminescence (NASBA-ECL). NASBA-ECL method was faster and hundredfold more sensitive than reverse transcription-polymerase chain reaction (RT-PCR) for ASSVd detection in apple leaves/ stems. ASSVd was rapidly transmitted to the entire tree in the second year after artificial inoculation. The ASSVd could be transmitted efficiently by using contaminated pruning scissors to both lignified stems (60 to $70\%$) and green shoots (20 to $40\%$) of apple tree and young plants. Dipping of contaminated scissors in $2\%$ sodium hypochlorite solution effectively prevented viroid transmission. In the ASSV d-infected fruits, the viroid was easily detected from fruit skin, seed coat, and embryo. Moreover, embryo and endosperm separately excised from the ASSVd-infected seeds were ASSVd positive in NASBA-ECL assay. Seedlings germinated from ASSVd-positive seeds showed $7.7\%$ infection rate., which indicated that ASSVd is seed-borne.

• The Plant Pathology Journal
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• 제38권4호
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• pp.313-322
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• 2022

Seed-borne pathogens in crops reduce the seed germination rate and hamper seedling growth, leading to significant yield loss. Due to the growing concerns about environmental damage and the development of resistance to agrochemicals among pathogen populations, there is a strong demand for eco-friendly alternatives to synthetic chemicals in agriculture. It has been well established during the last few decades that plant seeds harbor diverse microbes, some of which are vertically transmitted and important for plant health and productivity. In this study, we isolated culturable endophytic bacteria and fungi from soybean seeds and evaluated their antagonistic activities against common bacterial and fungal seed-borne pathogens of soybean. A total of 87 bacterial isolates and 66 fungal isolates were obtained. Sequencing of 16S rDNA and internal transcribed spacer amplicon showed that these isolates correspond to 30 and 15 different species of bacteria and fungi, respectively. Our antibacterial and antifungal activity assay showed that four fungal species and nine bacterial species have the potential to suppress the growth of at least one seed-borne pathogen tested in the study. Among them, Pseudomonas koreensis appears to have strong antagonistic activities across all the pathogens. Our collection of soybean seed endophytes would be a valuable resource not only for studying biology and ecology of seed endophytes but also for practical deployment of seed endophytes toward crop protection.