• Title/Summary/Keyword: seed variability

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Seed Variability Test by 2,3,5-Triphenyltetrazolium Chloride (염화(鹽化)트리페닐테트라졸륨에 의(依)한 종자활력검정(種子活力檢定)에 관(關)한 연구(硏究))

  • Kim, Sam-Sik
    • Journal of Korean Society of Forest Science
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    • v.26 no.1
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    • pp.23-30
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    • 1975
  • To elucidate the dependability of seed variability determination by tetrazolium, 15 tree species were checked. For the comparison of potential germinability for these tree seeds, x-ray photography and routine germination test in a incubator were implemented. Though there were considerable differences of seed variability in percentage between germination test and tetrazolium test, the judgement by tetrazolium test and x-ray test gave similar results. These findings indicated that tetrazolium test is a reliable method if treated and inferred properly. Solution of 0.25 percent tetrazolium could give a complete colour reaction as well as 0.50 percent solution.

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Incidence and variability of Hosta virus X and seed-transmission in Hosta plants

  • Park, M.H.;Lee, J.S.;K.H. Ryu
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.144.2-145
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    • 2003
  • This study was performed to investigate the incidence of Hosta uirus X (HVX), a Potexvirus, from cultivated hosta ornamental plants in Korea and to ascertain seed transmission of the virus from infected parent plant to progeny ones for breeding program of hosta plants. Infection rate of HVX in cultivated hostas was 25.6 % (11 out of 43 collected samples contained HVX) based on Western blot and RT-PCR detection methods. Most of HVX-infected hostas showed visible systemic leaf symptoms (mosaic, mottle, curling, stunting or combinations). Variability of HVX was confirmed by sequences of coat protein gene of individual isolates from different hostas. HVX was seed-transmitted on Hosta 'Blue Cadet'. The virus was detected from seeds, and sprouts and seedlings from the virus-contaminated seed sources. Over 7.5 % of seeds were HVX-contaminated surveyed in this study, Our data suggest that HVX can be transmitted by seed source, and indexing of the virus should be done for breeding program of Hosta.

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Effects of PEG Priming Treatment on Germination and Seedling Growth of Onion Seed(Allium cepa L.)

  • Lee, Sheong-Chun;Ahn, Chan-Young
    • Plant Resources
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    • v.2 no.2
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    • pp.88-95
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    • 1999
  • These experiments were conducted to evaluate the variability of seed germination and seedling growth with different levels of polyethylene glycol(PEG 6,000) solution in onion seed. Average germination percentage of seed primed in PEG solution with 1.00 and 0.75 MPa was higher than control, and that of seed primed in 1.50MPa was lower than unprimed control. Germination percentage(GP) of seed primed for 5 days was highest, and as the primed days become long, the GP was decreased. The GP of airation seed during the primed was higher than that of unairation seed, about 5% , respectively. The GP of washed seed after primed was higher than that of unwashed seed, but that of redried seed after primed was lower than that of the others. The highest GP cultivar was Chunjoogoohyung and the lowest GP cultivar was Seouldego in unredried seed after primed, but Chunjoojoonggo was highest and Jungpoonwhang was the lowest cultivar in redried seed after primed. As the PEG concentration increased, the seedling length(SL) was shortened, and seed primed for 15 days was longer than other treatments. The SL of primed seed was similar to GP. The SL of washed seed after primed was longer than that of others, but that of redried seed after primed was shortest among the others. The SL of Chunjoojoonggo and Nongwoodego was longest and Seouldego was shortest among the cultivars in unredried seed after primed, but that of Chunjoogoohyung and Chunjoojoonggo was longest and Seouldego was the shortest cultivars in redried seed after primed.

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Genitic Variability and Correlation of Quantitative Characters in Local Garlic Cultivars (在來種마늘의 量的形質에 대한 遺傳變異와 相關)

  • 김정선
    • Korean Journal of Plant Resources
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    • v.11 no.3
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    • pp.264-271
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    • 1998
  • This study was conducted to investigate morphological characteristics of Korean local garlic varieties and to define the genetic variability and the correlations amongthe characters associated with yield. The plant height, the leaf sheath diameter, the nubmer of leaves, and bulb weight were significantly increased in the garlic plants from heavier seed bulbs. The proportation of six clove bulbs was more than 60% in Seosan variety and this was not realted with the number of cloves in seed bulb. While more than 70% bulbs in Jeoksung and Danyang varieties cultivated in paddy field were composed of 6 or 7 cloves, majority of the bulbs of Euisung and Danyang varieties cultivated in upland were composed of bulbs with 7 cloves or more. The highest genotypic coefficient of variation (GCV) and phenotypic coefficient of variation (PCV) were observed in bulb weight. GCV and PCV also showed high value in leaf sheath diameter. Thus, there is a greater scope for selection of these characters. The small difference between PCV and GCV values in plant height indicates that the environmental influence would be limited in this character. High heritability was observed of plant height (56.76%) and bulb weight (45.95%). And the weight per bulb (34.24%) exhibited highest genitic advance followed by leaf sheath diameter (18.8%) and plant height (9.61%), and those would be the ideal characters for selelction.

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Variability of Azadirachtin in Azadirachta indica (neem) and Batch Kinetics Studies of Cell Suspension Culture

  • Prakash Gunjan;Emmannuel C.J.S.K.;Srivastava Ashok K.
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.3
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    • pp.198-204
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    • 2005
  • Seeds of neem were collected from different parts of India and analyzed for their azadirachtin content by High Performance Liquid Chromatography (HPLC). In order to assess the effects of genotypic and geographical variation on azadirachtin content in cell cultures, callus development was attempted in the seeds containing high and low concentration of azadirachtin. The concentration of azadirachtin in callus cultures was significantly affected by the explant source. Seed kernels with higher azadirachtin content produced higher azadirachtin content in callus cultures and lower azadirachtin content was seen in callus cultures produced from seed kernels with low azadirachtin content. The protocol for development of elite stock culture of Azadirachta indica was established with the objective of selecting a high azadirachtin-producing cell line. The highest azadirachtin-producing cell line was selected and the effects of different media and illumination conditions on growth and azadirachtin production were studied in shake flask suspension culture. Detailed batch growth kinetics was also established. These studies provided elite starter culture and associated protocols for cultivation of A. indica plant cell culture in the bioreactor.

Germination Characteristics of PEG Priming Seed in Barley (Hordeum vulgare L.) (PEG로 프라이밍 된 보리(Hordeum vulgare L.)종자의 발아특성)

  • 이성춘;박문수;배창휴
    • Korean Journal of Plant Resources
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    • v.15 no.1
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    • pp.18-25
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    • 2002
  • These experiments were conducted to evaluate the variability of seed germination, and seedling growth of PEG priming barley. The average germination percentage(AGP) of PEG priming seed was higher than control, but those were low with extend the treatment period. The AGP of washing seed after PEG priming was higher than unwashed seed, and that of redried seed after priming was lower than control. The germination time of priming seed was short compare to control seed, and that was prolonged with extend the priming period, and that of washing seed after priming was shortening, and that of redried seed after priming was prolonging. The emergence percentage(EP) of priming seed was higher than control, and the emergence time was shortest in 50% field moisture capacity soil. The seedling and root length of priming seed was shorter than control.

Morphological Variations in Tetrapleura tetraptera Taub. (Fabaceae) Fruits and Seed Traits from Lowland Rainforest Zones of Nigeria: A Keystone Non Timber Forest Tree Species in the Tropics

  • Aishat Adeola Olaniyi;Samuel Olalekan Olajuyigbe;Musbau Bayo Olaniyi
    • Journal of Forest and Environmental Science
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    • v.40 no.2
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    • pp.111-117
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    • 2024
  • An evaluation was carried out on variability in morphology of fruits and seeds (number and weight) of Tetrapleura tetraptera (Schumach. and Thonn.) Taub. from different populations across its distribution range in Nigeria. Bulk fruit samples were collected and examined for variations in morphological characters. Differences in morphological character of fruits and seeds among the populations were determined using analysis of variance at 5% level of probability. The relationships among morphological characters were determined using Pearson correlation coefficient (r). Significant variations (p<0.05) existed among T. tetraptera populations for all the evaluated characters: fruit length, fruit width, number of seeds per fruit and seed weight. A positive significant strong correlation (r=0.96) was found between seed weight and number of seeds per fruit, while no correlation existed between fruit length, width and number of seeds. Seed weight was positively correlated with minimum altitude (r=0.97) and maximum altitude (r=0.99) of seed populations. Number of seeds was also significantly correlated with maximum altitude (r=0.965). There was no significant correlation between geo-climatic variables and fruit dimensions (length and width). Observed variations in morphological traits within and across populations of T. tetraptera may be used as proxy to estimate genetic diversity and selection of superior trees for improved productivity.

A pilot study on the radio flux variability of dwarf galaxies

  • Hwang, Ji-Hye;Woo, Jon-Hak;Jung, Taehyun;Chung, Aeree;Trippe, Sascha;Baek, Junhyun;Lee, Taeseok;Park, Dawoo
    • The Bulletin of The Korean Astronomical Society
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    • v.39 no.2
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    • pp.71.1-71.1
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    • 2014
  • The black hole occupation fraction in dwarf galaxies can provide an important clue for understanding the black hole seed formation. As a pilot feasibility study, we performed a KVN radio monitoring campaign over 8 months for 4 dwarf galaxies. Two galaxies (IC10 and NGC1569) are detected at 22 GHz, respectively with 39 mJy, 83 mJy. The measured flux (rms) variability is 13% and 8%, respectively for IC10 and NGC1569, while the mean flux uncertainty is 25% and 12%. Thus, the detection of the radio flux variability is at best marginal. Detecting flux variability of faint sources (i.e., 22 GHz flux < 200 mJy) seems challenging with the KVN single dishes. Combining with the 1.4 GHz flux measurements from the NVSS, we find that these two galaxies have a steep spectrum, supporting that the radio sources are AGNs. Instead of a monitoring, single-epoch multi-band observations can be effective for identifying radio AGNs by providing the constraint of the radio continuum slope.

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Performance of Heritabilities, Genetic Correlations and Path Coefficients of Some Agronomic Traits at Different Cultural Environment in Sesame

  • Shim, Kang-Bo;Kang, Chul-Whan;Lee, Sung-Woo;Kim, Dong-Hee;Lee, Bong-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.4
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    • pp.245-250
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    • 2000
  • This study was conducted to analyze the responses of some agronomic traits to the different cultural environments and relations among the agronomic traits for selecting sesame varieties with higher cultural stabilities. The indexes for stability parameters measured were coefficient of variability, heritabilities, genetic correlations and path coefficients of agronomic traits according to locations and years in Korea. The heritabilities of agronomic traits showed different by locations and years. Number of seeds per capsule and 1000 seeds weight showed higher heritabilities, but stem length and seed weight per plant showed relatively lower heritabilities. Average heritabilities of some agronomic traits in 1998 were comparatively higher than those of 1999. Of six areas, Jinju area showed biggest coefficient of yield variability in 1998-1999. Iksan and Taegu areas showed higher heritabilities in 1998, but Iksan and Jinju areas showed lower heritabilities in 1999. Genetic correlations were slightly higher than corresponding phenotypic correlations. Stem length showed positive genetic correlation with the number of capsules per plant, and seed weight per plant and the number of capsule per plant showed positive genetic correlation with seed weight per plant. On the analysis of path coefficients, stem length and number of capsules effected highly on grain yield. Great regional variations were observed on the effects of agronomic traits on grain yield. Higher direct effects of stem length on grain yield were observed at Suwon, Chungwon, Taegu, Jinju and Naju areas, but in Iksan area was observed higher direct effect of the number of capsules per plant on grain yield in 1998. In 1999, higher direct effect of stem length on grain yield was observed at Chungwon and Suwon areas. Iksan and Taegu areas were also observed higher direct effect of the number of capsule per plant on grain yield.

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Effects of Seed Size and Temperature on Hypocotyl Elongation in Mungbean (녹두품종별 종실크기 및 온도처리에 따른 하배축 신장성)

  • 이성춘;김동철;임태곤;송동석
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.6
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    • pp.634-639
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    • 1996
  • This experiment was conducted to evaluate the variability of hypocotyl elongation of mungbean varieties. With four mungbean cultivars, which were classified as 4234-697 and Keumsungnogdu(long), Nampyungnogdu(Medium), and Seonhwanogdu(short), hypocotyl elong-ation was measured 4 to 6 days after seeding in paper towel at different temperatures (15, 20, 25, 30, and 35$^{\circ}C$). Hypocotyl elongation of mungbean seed stored at 5$\pm$1$^{\circ}C$ for 6 months was compared with of seed stored at room temperature. As the temperature rises, the hypocotyl is longer. The hypocotyl elongation started immediately at high temperature, and longest at the range of 30 to 35$^{\circ}C$. The hypocotyl elongation became longer at the 5$^{\circ}C$ storage plot than at the room temperature plot. Correlation coefficient (r) between 100 seed weight and hypocotyllength are not significant.

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