Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Variability of Azadirachtin in Azadirachta indica (neem) and Batch Kinetics Studies of Cell Suspension Culture

  • Prakash Gunjan (Plant Cell Culture Laboratory, Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi) ;
  • Emmannuel C.J.S.K. (Arid Forest Research Institute) ;
  • Srivastava Ashok K. (Plant Cell Culture Laboratory, Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi)
  • Published : 2005.06.01
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Abstract

Seeds of neem were collected from different parts of India and analyzed for their azadirachtin content by High Performance Liquid Chromatography (HPLC). In order to assess the effects of genotypic and geographical variation on azadirachtin content in cell cultures, callus development was attempted in the seeds containing high and low concentration of azadirachtin. The concentration of azadirachtin in callus cultures was significantly affected by the explant source. Seed kernels with higher azadirachtin content produced higher azadirachtin content in callus cultures and lower azadirachtin content was seen in callus cultures produced from seed kernels with low azadirachtin content. The protocol for development of elite stock culture of Azadirachta indica was established with the objective of selecting a high azadirachtin-producing cell line. The highest azadirachtin-producing cell line was selected and the effects of different media and illumination conditions on growth and azadirachtin production were studied in shake flask suspension culture. Detailed batch growth kinetics was also established. These studies provided elite starter culture and associated protocols for cultivation of A. indica plant cell culture in the bioreactor.

Keywords

References

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