• Journal of Microbiology and Biotechnology
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• v.15 no.4
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• pp.903-908
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• 2005

Inoculation of the carrier-based mixed bioinoculants af N-fixer (Azospirillum lipoferum strain Az204/Rhizobium strain BMBS P47) and phosphate-solubilizing bacterium (Bacillus megaterium var phosphaticum strain Pb 1) promoted growth and yield of pearl millet and blackgram under pot-culture conditions. The mixed inoculant of Az204 and Pb 1 enhanced germination, seedling vigor, plant height, and seed weight, and resulted in $6\%$ increase in grain yield of pearl millet. Likewise, the mixed inoculant of BMBS P47 and Pb1 increased growth, nodulation, and yield in blackgram. The rhizosphere soil enzyme activities, including nitrogenase, urease, and phosphatase, in both pearl millet and blackgram were significantly increased by the inoculation of the mixed inoculant, compared to that of the individual inoculants. The results clearly indicate the beneficial effect of co-culturing the N-fixer and P-solubilizer in inoculants production.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.10
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• pp.79-83
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• 1971

To investigate the effects of seed treatments and liming on nodulation and growth of lucerne(Medicago sativa L.) in an upland yellow-brown earth in N.Z. the five levels of seed treatments(Uninoculated, Inoculated, Inoculated plus lime pelleted, Inoculated plus 50/50 phosphate/dolmite pelleted, Inoculated plus ta1c pelleted), and 2 levels of lime applications (0, 25kg/10a) were compared and their interactions were observed, and then the following results were obtained. 1. In oder to obtain the effective nodulation of lucerne on acid soil it is usually necessary to apply lime and inoculate the seed. 2. The coating of inoculated lucerne seed with adequate materials will promote the nodulation. 3. The pelleting of lucerne seed may be a of reducing the need for the heavy liming. 4. The finely grounded lime was the best to use origin upland yellow-brown earth. 5. When the seed were not inoculated or pelleted with inferior material, liming is very effective for the nodulation and growth of lucerne. 6. The pelleting seed will reduce the laborious work and expenditure.

• Research in Plant Disease
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• v.29 no.2
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• pp.184-187
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• 2023

In May 2020, we surveyed disease occurrence on vegetables grown in Seosan area, Korea. During the disease survey, white rot symptoms were observed in Korean wild chive (Allium monanthum) plants growing in fields. The symptoms occurred mainly in the seed bulb-producing fields of the crop. The above ground parts of the diseased plants displayed premature yellowing and dying of older leaves and stunting of the plants. The bulbs and roots of the diseased plants turned black and rotted. The disease occurred in a range of 1-60% in four of the eight fields surveyed. Three isolates of Sclerotium sp. were obtained from the bulb lesions of diseased plants. All isolates were identified as Stromatinia cepivora based on the morphological characteristics and phylogenetic analysis. Pathogenicity of the isolates on Korean wild chive was confirmed by artificial inoculation test. The lesions induced by the inoculation test were similar to those observed in the investigated fields. This is the first report of S. cepivora causing white rot in Korean wild chive.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.26 no.2
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• pp.198-206
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• 1981

A field research was conducted to evaluate the effects of inoculation of Rhizobium meliloti and lime application on the early growth of alfalfa, var. Luna, in an acid soil. Alfalfa seeds were inoculated by lime-peat pelleting method with $A_3$ strain isolated from alfalfa roots. Both inoculated and noninoculated seeds were sown in acid soil (pH 5.5) with three levels of lime application (1, 3, 6 ton/ha) on mid-April. Inoculated seeds produced many effective nodules in early stage and increased nitrogen and chlorophyll contents of leaf, consequently stimulating alfalfa growth. Pseudo nodules were found in noninoculated, while they were seldom formed in inoculated. Lime application increased soil pH, plant height, root length, and shoot and root weight. Liming effect on nodulation was significant at first cut, but no longer after then. Both inoculation and liming increased the forage yield of alfalfa. Inoculation and liming of 6 ton/ha together produced the highest yield. As compared to control plot of nitrogen application, the effect of seed inoculation only could be equal to about 120 kg/ha nitrogen application. The total nitrogen contents of forage were little affected by number or weight of nodules and amounts of lime application.

• Research in Plant Disease
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• v.28 no.2
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• pp.108-111
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• 2022

In December 2020, stem and leaf rot symptoms in small-fruited tomato (Solanum lycopersicum) plants were observed in a farmer's vinyl greenhouse located in Pyeongtaek, Gyeonggi Province, Korea. The incidence of diseased plants in the vinyl greenhouse was 2-6%. Seven single-spore isolates of Phoma sp. were obtained from the diseased stems and leaves. All the isolates were identified as Boeremia linicola based on the cultural, morphological and molecular characteristics. Two isolates of B. linicola were tested for pathogenicity on stems and leaves of small-fruited tomato and large-fruited tomato using artificial inoculation. All the tested isolates caused stem and leaf rot symptoms in the inoculated plants. The symptoms were similar to those observed in plants from the vinyl greenhouse investigated. This is the first report of B. linicola causing stem and leaf rot in tomato.

• Research in Plant Disease
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• v.9 no.1
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• pp.39-41
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• 2003

Bacterial soft rot by Erwinia carotovora subsp. carotovora is one of the diseases causing the biggest damages in Chinese cabbage cultivation. This study was conducted to evaluate disease resistance of Chinese cabbage cultivars and breeding lines to E. carotovora subsp. carotovora by new inoculation method, mineral oil inoculation method, inoculating 10 ml of the mixture (4:1, v/v) of bacterial suspension and mineral oil on the central bases of Chinease cabbage seedling. Total 43 Chinese cabbage cultivars and lines obtained from 3 domestic seed companies and universities were screened for disease resistance using the above mentioned inoculation method. This screening test showed that Chinese cabbage C3-26, C3-28, C3-29 and C29-51-51-53 lines were resistant, Gangta, Gumchonyealgali, Mini, DB50, Jibu, Pyungchng, Sanchon and Yellow King No.2 cultivars were susceptible, and the others were moderate resistant.

• Korean Journal Plant Pathology
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• v.14 no.5
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• pp.484-490
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• 1998

Microorganisms associated with seed-rhizome rot of gingers preserved in three underground storage caves were identified with respect to rot types. Rot patterns were grouped into 4 different types : yellow soft rot, brown rot, localized ring rot, and water-soaked rot. Water-soaked rot was highest in frequency with 40% and ring rot the least with 14%. Causal pathogens differed with rot type, yellow soft rot by Erwinia carotovora and Pseudomonas aeruginosa, brown rot by Fusarium solani and Pseudomonas aeruginosa, localized ring rot by F. solani, and water-soaked rot by Pythium spinosum and P. ultimum. Pythium myriotylum, the causal pathogen of ginger rhizome rot which occurs severely in fields was rarely detected from storage seed-rhizomes suggesting its minor involvement with storage rot. Pathogenic Pythium isolates were frequently obtained from both rhizome surface and inner tissues of rotten rhizomes. Detection frequency of Pythium isolates in inner tissues decreased as increasing distance from rhizome surface. In wound-inoculation tests, above pathogens caused a varying degree of rot on healthy rhizomes at 15$^{\circ}C$, 2$0^{\circ}C$ and 3$0^{\circ}C$ with increasing severity at higher temperatures.

• The Plant Pathology Journal
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• v.33 no.4
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• pp.424-428
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• 2017

Tomato yellow leaf curl virus (TYLCV) infection of the common bean (Phaseolus vulgaris) has been reported, but soybean (Glycine max) has not previously been identified as a TYLCV host. Five cultivars of white soybean were agro-inoculated using an infectious TYLCV clone. At 30 days post-inoculation, they showed infection rates of 25% to 100%. Typical TYLCV symptoms were not observed in any inoculated plants. To examine whether TYLCV was transmitted in soybean seeds, DNA was isolated from bundles of five randomly selected seeds from TYLCV-inoculated soybean plants and amplified with a TYLCV-specific primer set. With the exception of one bundle, all bundles of seeds were verified to be TYLCV-infected. Virus dissemination was also confirmed in three of the 14 bunches. Viral replication was also identified in seeds and seedlings. This is the first report demonstrating that soybean is a TYLCV host, and that TYLCV is a seed-transmissible virus in white soybean.

• Korean Journal Plant Pathology
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• v.2 no.3
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• pp.185-192
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• 1986

Upon germination, a conidium was septated in accordance with number of germ tubes. The percentages of ppressorial formation were not different between the resistant cultivars (Kumchang NO. 2 and Hongilpum) and the susceptible cultivars (Haneulcho and Saegochu). Appressorial form was various such as round, elliptic or star-shapped. The maximum number of appressoria was shown at 12 hours after inoculation. By 24 hours fter inoculation, hypersensitive tiny brown lesions were observed on the leaves and fruits of the resistant cultivars. Epidermal cells under cuticle layer of the resistant Kumchang NO. 2 fruit showed severe plasmolysis, while on the susceptible cultivars, the lession was largely extended to following incubation. Subcuticular infection hyphae were profusely colonized in the disintegrated tissues. Acervuli and setae on the stromatic structure ere formed at 96 hours. The infected seed coat was not only severely collapsed, but also infection hyphae were observed on the disintegrated seed coat, resulting severe plasmolysis of nucellus and embryo.

• Korean Journal Plant Pathology
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• v.13 no.4
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• pp.244-247
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• 1997

The experiment was conducted to obtain a basic information on the enviroumental factors favoring the disease development and chemical control of clubroot in chinese cabbage. The inoculation by insertion of infested soil was the most effective for the disease development as compared to the other inoculation methods such as pouring the spore suspension into soil and dipping roots into the spore suspension. On the environmental factors favoring the clubroot development, optimum slil pH and soil temperature were pH 5-6 and $20{\sim}30^{\circ}C$, respectvely. The combination of soil treatment and seed coating treatment was more effective than each single treatment in the chemical control of clubroot by 0.5% of fluazinam dust.