• Natural Product Sciences
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• v.25 no.1
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• pp.38-43
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• 2019

Microbial wound infection prolonged the hospitalization and increase the cost for wound management. Silver is commonly used as antimicrobial wound dressing. However, it causes several adverse side effects. Hence, this study was aimed to evaluate the antimicrobial efficiency of Swietenia macrophylla seed extract on clinical wound pathogens. Besides, the bioactive constituents of the seed extract were also determined. S. macrophylla seeds were extracted with methanol by maceration method. The seed extract inhibited 5 test bacteria and 1 yeast on disc diffusion assay. The antibacterial activity was broad spectrum, as the extract inhibited both Gram positive and Gram negative bacteria. On kill curve analysis, the antibacterial activity of the seed extract was concentration-dependent, the increase of extract concentration resulted in more reduction of bacterial growth. The extract also caused 99.9% growth reduction of Bacillus subtilis relative to control. A total of 21 compounds were detected in gas chromatography- mass spectrometry analysis. The predominant compounds present in the extract were oleic acid (18.56%) and linoleic acid (17.72%). In conclusion, the methanolic extract of S. macrophylla seeds exhibited significant antimicrobial activity on clinical wound pathogens. Further investigations should be conducted to purify other bioactive compounds from the seeds of S. macrophylla.

• Korean Journal of Medicinal Crop Science
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• v.13 no.4
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• pp.150-153
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• 2005

Although each part of Trichosanthes kirilowii is frequently used as medicinal herbs, study on the chemical composition is not sufficient. It was found that sarcocarp consists of 70% of carbohydrate, 13% of crude protein, 5% of crude fat, 6% of crude fiber and 6% of crude ash; seed consists of 62.59% of carbohydrate, 12.75% of crude protein, 14.80% of crude fat, 6.50% of crude fiber and 3.36% of crude ash; and root consists of 89.40% of carbohydrate, 4.10% of crude protein, 0.50% of crude fat, 3.50% of crude fiber and 2.50% of crude ash. Sarcocarp and seed contain fifteen kinds of amino acids such as Asp, Ser, Glu, Gly, His, Arg, Thr, Ala, Pro, Try, Cys, Met, Val, Leu, and Phe. Sarcocarp contain large quantities of phenylalanine and glycine and seed does not contain leucine but lysine. The mineral contents in Trichoxanthes kirilowii are 0.55% Ca, 0.91% Mg, 10.29% Na, and 0.17% K.

• Journal of Animal Science and Technology
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• v.55 no.2
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• pp.123-130
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• 2013

Whole cotton seed (WCS) has become one of the major feed ingredients in TMR for dairy cattle in Korea, and WCS for feed use is mostly imported from abroad. Since this genetically modified oil seed is usually fed to the animal in raw state, its germination ability, if last long, often causes concerns about ecological disturbances. In the process of looking for effective conditions to remove germination ability of WCS this study had the objectives to evaluate the nutritional effects of gamma irradiation at doses of 8, 10 and 12 kGy on changes in nutrient contents, anti-nutritional factors, in vitro digestibility and ruminal degradability. No significant differences were found in proximate analysis of nutrients between raw WCS and gamma irradiated one. Glycine and threonine contents significantly increased when the WCS was exposed to gamma ray as compared to untreated WCS (p<0.05). As for fatty acid composition, no significant differences were observed with the irradiation treatment. Free gossypol in WCS was decreased (p<0.05) by gamma irradiation treatment. Of the 3 different levels of gamma irradiation, a dose of 12 kGy was found to be the most effective in reducing free gossypol concentration. Results obtained from in situ experiment indicated that gamma irradiation at a dose of 10 kGy significantly (p<0.05) lowered rumen degradability of both dry matter and crude protein as compared with raw WCS. However, there were no significant differences in rapidly degradable and potentially degradable fractions of crude protein due to 10 kGy gamma irradiation. Overall, this study show that gamma irradiation at a dose of 10 kGy is the optimum condition for removing germination ability of WCS, and could improve nutritive value for the ruminant with respect to the decrease in both ruminal protein degradability and gossypol content of WCS.

• Journal of the Korean Applied Science and Technology
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• v.3 no.2
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• pp.17-22
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• 1986

Compositional and quantitative changes of 4-desmethylsterols in soybean (Glycine max. cv. Gangrim) seed and pod at six stages of development from flowering were investigated. The content of 4-desmethyl-, 4-monomethyl- and 4,4-dimethylsterols in developing soybean seed and pod was found to vary between 0.16-0.35, 0.04-0.12 and 0.04-0.06%, respectively. The predominating 4-desmethylsterols were sitosterol, campesterol and stigmasterol. The proportion of sitosterol in seed decreased to 57.0% with maturation, while in pod increased up to 88.7%. ${\triangle}^{7,22,25}$ Stigmastatrienol estimated 7.5% was detected in the fully mature seed.

• Asian-Australasian Journal of Animal Sciences
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• v.6 no.1
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• pp.123-126
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• 1993

Hand dibbling in rows produced higher (p < 0.05) fresh and dry matter (DM), fodder yield, higher plant density and cob yield than broadcasted. However, the varying seed rates (40, 50 and 55 kg/ha) did not affect (p < 0.05) the fresh and DM yield and cob yield except for 30 kg/ha seed rate which produced the lowest (p < 0.01). However, the overall average fresh fodder yield and the fresh and DM yield of cob were 24.97, 10.04 and 6.90 t/ha respectively. The overall average plant height (cm), cob/plant (nos.) plant/ha, (nos.) cob/ha (nos.) and weight (g) of one cob were 178, 1.11, 664141, 70104 and 145 respectively for both the sowing methods and the four seed rates. Crude protein contents of maize plant and cob by-products (8.12% and 7.34% respectively) indicated promising cattle feeds along with human food.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.4
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• pp.211-217
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• 1992

This experiment was conducted over a 3-year period (1990-1992) to evaluate the production and quality of orchardgrass (Potomac)-red clover (Kenland) mixture when orchardgrass + red clover mixture was sown at seed rates of 20+0. 18+2, 16+4, 14+6 and 12+8 kg/ha. Total herbage dry matter, crude protein and digestible organic matter production and organic matter intake were markedly affected by increasing rcd clover seed rate, those increased with increasing red clovcr seed rate due to the cnhancernent of red clover percentage of total herbagc in all 3-year. but significant differences occured between the orchardgrass alone and orchardgrass-red clover mixture with a seed rate of 4,6 and 8 kg/ha red clover (P<0.01). But on the other hand increasing red clover seed rate increased red clover percentage in total herbage, the mean red clover percentage of total herbage at the last cutting ranged from 54 to 65% in first year. 58 to 60% in second year, and 62 to 69% in third year, particularly it was observed that red clover was sown at seed rates of 6 and 8 kg/ha for red clover sown dominated strongly as the cutting times were progressed. Considering the maintenance of good botanical composition balance and the enhancement of production and quality of herbage, the optimized seed rate for orchardgrass-red clover mixtures, 16 kg/ha orchardgrass and 4 kg/ha red clover seems to be considered adequate.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.10 no.6
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• pp.99-109
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• 2007

The main objectives of this comparative study were 1) to compare the floristic similarity of species composition between the extant vegetation and seedlings from soil seed bank and 2) to quantify the potential contribution of soil seed bank to revegetation of forest in a constructed area, which is called "ecological impact mitigation" in conjunction with the power plant extension. Forest topsoil of seven plots was collected from the surface soil after measurements were taken on the ground vegetation in each plot. A greenhouse experiment was conducted and monitored to analyze the germination potential of soil seed bank. The forest topsoil was spread on plastic trays ($0.7m^2{\times}7$) filled with a 5cm layer of sterilized potting mix. The results of monitoring for 2 years in a greenhouse were as follows : 1) seedlings of soil seed bank per 4.9$m^2$ were 1,269 with 36 species (1st year) and 2,615 with 25 species (2nd year). 2) 38${\pm}$8% of the flora species were germinated from soil seed bank. It can be concluded that the use of soil seed bank would be effective to promote establishment of diverse species and vegetation. However, it behooves to continue monitoring on succession of vegetation and pursue revegetation with other methods for ecological restoration. Finally, adequate topsoil deposit and gathering methods should be studied properly.

• Journal of the Korean Home Economics Association
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• v.24 no.3
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• pp.79-84
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• 1986

the purpose of this paper is to study on the proximate composition, mineral and saponin content in white sesame, black sesame, and perilla seed. For this purpose, we have compared six different materials: white-raw-sesame, white-roast-sesame, black-raw-sesame, black-roast-sesame, raw-perilla seed and roast perilla seed, and have come to the following results. The crude fat content was the highest in white-raw-sesame(55.3%). In all the six samples, the crude fat content in raw seeds was all higher than that in roast seeds. The crude protein content was the highest in the roast perilla seed(24.6%), and in the six samples, the crude protein content in roast seeds all higher than that in roast seeds. The total sugar content was found to be the highest in the roast perilla seed(8.29%). The reducing sugar content was higher in raw perilla seed(1.57%) than in other sample materials. The ash content was the highest in black raw-sesame(5.93%), and that percentage rates was the same as that of FAO and of Japan. Minerals like Cd. Mn. Cu. Na. Mg. Pb. and Ca. were found to be contained more in black sesame than in other sample materials. The minerals contained most in white sesame were Zn.(61.6ppm) and Fe(49.4ppm), and K was contained a little more in perilla seed than in the others. The sample materials which contain saponin most were white-roast-sesame(0.34%) and black-roast-sesame(029%).

• Journal of the Korean Society of Food Science and Nutrition
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• v.16 no.2
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• pp.118-122
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• 1987

In order to find the effect of the storage time on the characteristics and sterol composition of the sesame seed, the characteristics, unsaponifiable matters, fractionation pattern of sterol and sterol compositions of 4-desmethylsterol on the sesame seed oil were analysed at the harvest time and after 56 months storage. The sample seed was kept in cotton bag within the natural dark store room. The oil content was decreased from 50.3g per 100g sesame seed at harvest time to 45.5g at 56 months stored. The acid value was increased sharply from 1.8 to 13.4 during the storage of sesame seed and iodine value was increased small, but saponification value and refractive index were hardly changed. The amount of unsaponifiables from 24.3mg to 20.9mg per gram on basis during storage time, and the 4,4-dimethylsterol and 4-monomethylsterol from unsaponifiables were decreased, otherwise, 4-desmethylsterol was increased. In the changes of sterol composition in the 4-desmethylsterol fraction from unsaponifiables, the compesterol and stigmasterol content were not nearly changed, otherwise, the sitosterol content was decreased from 51.0% to 48.2%, but the content of ${\Delta}^5-avenasterol$ was increased from 15.2 % to 18.4% during the storage of sesame seed within 56 months.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.2
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• pp.182-188
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• 1988

In order to find out the effects of the time and degree of defoliation on the seed yield, oil content and fatty acid composition of perilla seed, two cultivars, Namhae and Geumnung, were grown in two years, 1985 and 1987. Defoliation was initiated after 110 days from sowing with two-week interval. Defoliation degree was manipulated into four levels i.e. 0, 25, 50 and 75 per cent based on the total leaf area per plant. The results are summarized as follows: Flowering date, maturing date, culm length, culm diameter and branch numbers were not significantly influenced by the defoliation time and degrees, but by the cultivars. The higher levels of defoliation was made, the lower seed yield and 1000-seed were obtained, and either the earlier or the later time of defoliation, the less influence on seed yield were observed for both cultivars. Severe defoliation caused a significant decrease in oil content. The composition of unsaturated and saturated fatty acids was significantly decreased by severe defoliation (above 25 per cent). In conclusion, with a view to achieving higher seed yield and oil content with good quality, it is advisable to defoliate perilla leaf less than 25 per cent based on total leaf area at either or later growth stages.