• Journal of Microbiology and Biotechnology
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• v.17 no.4
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• pp.586-593
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• 2007

Seed coating by a phenazine-producing bacterium, Pseudomonas chlororaphis O6, induced dose-dependent inhibition of germination in wheat and barley seeds, but did not inhibit germination of rice or cucumber seeds. In wheat seedlings grown from inoculated seeds, phenazine production levels near the seed were higher than in the roots. Deletion of the gacS gene reduced transcription from the genes required for phenazine synthesis, the regulatory phzI gene and the biosynthetic phzA gene. The inhibition of seed germination and the induction of systemic disease resistance against a bacterial soft-rot pathogen, Erwinia carotovora subsp. carotovora, were impaired in the gacS and phzA mutants of P chlororaphis O6. Culture filtrates of the gacS and phzA mutants of P. chlororaphis O6 did not inhibit seed germination of wheat, whereas that of the wild-type was inhibitory. Our results showed that the production of phenazines by P. chlororaphis O6 was correlated with reduced germination of barley and wheat seeds, and the level of systemic resistance in tobacco against E. carotovora.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.83-83
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• 2017

The National Agrobiodiversity Center (NAS, RDA, Republic of Korea) has continually collected new valuable genetic resources. In this study, we regenerated conserved kidney bean (Phaseolus vulgaris L.) germplasm which couldn't be available because of seed quantity and quality, and we also surveyed their morphological characters for the sustainable utilization. A total of 431 kidney bean accessions were regenerated and 18 morphological traits were surveyed according to the characterization guideline of RDA Genebank. Among the surveyed traits, flowering time ranged from May 23 to September 4 and 73.8% of tested accessions were mainly flowering in June. The maturity time ranged from July 1 to October 15 and main flowering time was July (91.4%). For plant type, 270 accs (62.6%) were climbing type followed by medium type of 86 accs (20.0%) and dwarf type of 65 accs (15.1%). The seed coat colors were various; yellow (34.6%), white (22.3%), brown (17.9%), red (10.7%), black (5.8%), violet (11%), pink (1.4%), navy (0.9%). Principal component analysis indicated that five principal components (PCs) with Eigen values >1 accounted for more than 65.8% variability. The first PC was more related to growth habits such as growth type, flowering time, and plant type. The second and third PCs showed higher values of the pigment characters such as seed coat color, flower color, and pod color. In fourth and fifty PCs, there were the higher positive values of the pod shapes. Our results provided insight into the characteristics kidney beans, thus the utilization basis of kidney beans might be elevated for bio-industry.

• Korean Journal of Breeding Science
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• v.40 no.4
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• pp.361-370
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• 2008

In order to better understand the morphological differentiation of the two cultivated types of Perilla crop and their weedy types in Korea and Japan, we studied the variation of 62 accessions by examining 15 morphological characteristics. By using ANOVA (one-way analysis of variance), we determined that var. frutescens and var. crispa showed significant morphological differences in terms of plant height and seed weight. Furthermore, cultivated var. frutescens and var. crispa could also be clearly discriminated from one another using PCA (principal component analysis). Specifically, quantitative and qualitative characteristics such as plant height, seed weight, degree of pubescence, shape of leaf, color of leaf, fragrance of plant, color of flower, color of stem and seed size greatly contributed to differences seen in the positive and negative direction on the first axis. In our study, most accessions of cultivated var. frutescens and those of its weedy type could be clearly discriminated from one another, however, most accessions of cultivated and weedy types of var. crispa were not clearly discriminated by the ANOVA and PCA analyses. These results indicated that cultivated var. frutescens can be considered to be a domesticated form, while the cultivated var. crispa can not be considered to be a domesticated form in Korea and Japan. It is our belief that our results concerning the morphological variations among cultivated types of Perilla crop and their weedy types in Korea and Japan will help ensure the long-term success of breeding programs and maximize the use of the germplasm resources in Korea.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.2
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• pp.202-213
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• 1997

In order to find out a high potential black soybean lines, of which demand has been increasing in Korea, twenty eight local cultivars were evaluated on agronomic characteristics and the classification of the cultivars was made by the cluster analysis. Days to maturity and days to growing of black soybeans were widely ranged from 58 to 82 days, and 117 to 148 days, respectively. Late maturing group showed over 140 maturity days, and heavy 100 seed weight. There was shown low yield potential in late maturing group, and growing days was positively correlated with flowering days, days to maturity, 100-seed weight and stem length, respectively. From the principal component analysis upper two components composed 76.5％ cumulative eigen value to total. Nine varietal groups were identified in relations to their affinity of the black soybeans. Selected black soybeans, Namhae-2 and Hamyang-l were field-tested and those characteristics of many pod, small seed and high yield were found out to be suitable for sprouting.

• Korean Journal of Plant Resources
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• v.33 no.4
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• pp.293-302
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• 2020

Morphological characteristics of Ziziphus jujuba accessions, including 'Ilbon', were investigated to verify the possibility of 'Ilbon' as a breeding material. 'Ilbon' has smaller leaves and fruits and a lower total soluble solids content compared to the major Z. jujuba accessions. However, total polyphenol content and antioxidant capacity were highest in 'Ilbon' among the Z. jujuba accessions. In addition, 'Ilbon' could produce intact seeds and seed productivity of 'Ilbon' was highest compared with major Z. jujuba accessions. Principal component analysis and clustering analysis using the morphological characteristics of Z. jujuba accessions have been classified them to geographical origin. In particular, 'Ilbon' was clearly distinguished from the major Z. jujuba accessions. Therefore, 'Ilbon', which has the highest seed productivity and functional component, will be used as a material for cross breeding and will promote genetic study of Z. jujuba.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.3
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• pp.317-322
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• 1997

In order to investigate vigor of rice seed pregerminated, some organic and inorganic component content, response of ecotypes, and effect of gibberellin treatment, under different levels of hastening of germination, were examined. Protein content was high in dried seed, the larger size in length of pregerminated seed, the less protein content, but lipid content was a reverse tendency to protein one. Potassium content was low in dried seed, the larger size in length of pregerminated seed, the more potassium content, but increase dramatically at above 10 mm in length of sprout. Magnesium content showed a same tendency to potassium one. The ability of regermination was high in early maturing cultivar, but almost lost in the pregerminated seeds with above 5 mm in length of sprout. The regermination percentage of dried seed was lower than that of pregerminated seed with 0.1∼0.5 mm in length, but was similar to that of pregerminated one with 1∼3 mm in length of sprout. The effect of gibberellin on regermination percentage was highest at 10 ppm and showed high with small sized one of pregerminated seed. The effect of gibberellin on plant height, fresh weight, and dry weight showed a same tendency to regermination percentage.

• Korean Journal of Medicinal Crop Science
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• v.8 no.2
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• pp.94-101
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• 2000

On the purpose of development of antioxidative compounds from natural sources, 38 plants known to have antioxidant activity have been examined concerning DPPH radical scavenging activity. Among 13 plants exhibiting the activity, the seed of Carthamus tinctorius L, was selected as resources to search for active compounds due to rareness of study. The seed of the plant has been used as edible oil or preventive and remedial drugs for osteoporosis, arthritis. To reveal the principal component manifesting the antioxidant activity, the MeOH extracts was successively solvent-fractionated with EtOAc, n-BuOH and water. In order to isolate active component from the EtOAc fractions, application of silica gel column chromatographies and activity tests were repeated for a active component to be isolated. Its chmical structure was determined to be N-feruloylserotonin, a conjugated serotonin compound, by the interpretation of spectral data, NMR. MS and the adaptation of chemical reactions.

• Journal of the Korean Society of Clothing and Textiles
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• v.18 no.5
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• pp.661-673
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• 1994

We have gathered a fatty acid to recycle the waste oil of food manufacturing process, and then made a soap from the waste oil by alkali saponification. Effects of the washing elements such as the concentration of the soap, temperature and time were evaluated to find out the optimum washing conditions, and results are as follows. 1. We could find soaps made from the soybean oil (A), corn oil (B), rape seed salad oil (C), cotton seed oil (D) and a soap consisting of the each oil 25% respectively (I) had the lowest surface tension at the concentration of 0.225% -0.25%. Compared with the single fatty-acid soap, the multi-component soap I showed the lower surface tension at the cmc. 2. All the samples of A-I showed the lowest contact angle for the solid material at the concentration of 0.25%. The multi-component soap of I showed higher contact angle than the single.component soaps of A, B, C and D. 3. The soap G, made from the waste oil, show lower surface tension than 5, made from the complex raw fats of the eatable fatty oil acid and H, commercial soap. 4. The washing efficiency depends on the washing time. Especially the 25-minute was found to be the optimum washing time. 5. The highest washing efficiency was found at the 0.25% cont. reagardless of the washing temperature. At 0.15% concentration level the washing efficiency reduces as washing tem- perature increases. At 0.3% concentration level the highest washing efficiency was found between $50^{\circ}$-$60^{\circ}$. 6. The soap made from the waste oil showed the highest washing efficiency in terms of concentration, temperature, and time. 7. The soap made from the waste oil showed the similiar concentration of hydrogen ion to the synthetic detergent. 8. The hand value of the fabric washed by the soap made from the waste oil was a little lower value than those washed by the synthetic detergent.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.11
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• pp.188-194
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• 2013

In this paper, we proposed a new method for pulmonary vessels image segmentation and refinement from pulmonary image. Proposed method consist of following five steps. First, threshold estimation is performed by polynomial regression analysis of histogram variation rate of the pulmonary image. Second, segmentation of pulmonary vessels object is performed by density-based segmentation method based on estimated threshold in first step. Third, 2D connected component labeling method is applied to segmented pulmonary vessels. The seed point of both side diaphragms is determined by eccentricity and size of component. Fourth step is diaphragm extraction by 3D region growing method at the determined seed point. Finally, noise cancelation of pulmonary vessels image is performed by 3D connected component labeling method. The experimental result is showed accurately pulmonary vessels image segmentation, the diaphragm extraction and the noise cancelation of the pulmonary vessels image.

• Journal of Microbiology and Biotechnology
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• v.17 no.12
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• pp.2027-2032
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• 2007

[ ${\alpha}$ ]-Synuclein is the major component of Lewy bodies and responsible for the amyloid deposits observed in Parkinson's disease. Ordered filamentous aggregate formation of the natively unfolded ${\alpha}$-synuclein was investigated in vitro with the periodic ultrasonication. The ultrasonication induced the fibrillation of ${\alpha}$-synuclein, as the random structure gradually converted into a ${\beta}$-sheet structure. The resulting fibrils obtained at the stationary phase appeared heterogeneous in their size distribution, with the average length and height of $0.28\;{\mu}m{\pm}0.21\;{\mu}m$ and $5.6\;nm{\pm}1.9\;nm$, respectively. After additional extensive ultrasonication in the absence of monomeric ${\alpha}$-synuclein, the equilibrium between the fibril formation and its breakdown shifted to the disintegration of the preexisting fibrils. The resulting fragments served as nucleation centers for the subsequent seed-dependent accelerated fibrillation under a quiescent incubation condition. This self-seeding amplification process depended on the seed formation and subsequent alterations in their properties by the ultrasonication to a state that accretes the monomeric soluble protein more effectively than their reassociation of the seeds back to the original fibrils. Since many neurodegenerative disorders have been considered to be propagated via the seed-dependent amyloidosis, this study would provide a novel aspect of the significance of the seed structure and its properties leading to the acce]erated amyloid formation.