• 제목/요약/키워드: seed analysis

검색결과 1,369건 처리시간 0.025초

The Role of Rice Vacuolar Invertase2 in Seed Size Control

  • Lee, Dae-Woo;Lee, Sang-Kyu;Rahman, Md Mizanor;Kim, Yu-Jin;Zhang, Dabing;Jeon, Jong-Seong
    • Molecules and Cells
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    • 제42권10호
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    • pp.711-720
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    • 2019
  • Sink strength optimizes sucrose import, which is fundamental to support developing seed grains and increase crop yields, including those of rice (Oryza sativa). In this regard, little is known about the function of vacuolar invertase (VIN) in controlling sink strength and thereby seed size. Here, in rice we analyzed mutants of two VINs, OsVIN1 and OsVIN2, to examine their role during seed development. In a phenotypic analysis of the T-DNA insertion mutants, only the OsVIN2 mutant osvin2-1 exhibited reduced seed size and grain weight. Scanning electron microscopy analysis revealed that the small seed grains of osvin2-1 can be attributed to a reduction in spikelet size. A significant decrease in VIN activity and hexose level in the osvin2-1 spikelets interfered with spikelet growth. In addition, significant reduction in starch and increase in sucrose, which are characteristic features of reduced turnover and flux of sucrose due to impaired sink strength, were evident in the pre-storage stage of osvin2-1 developing grains. In situ hybridization analysis found that expression of OsVIN2 was predominant in the endocarp of developing grains. A genetically complemented line with a native genomic clone of OsVIN2 rescued reduced VIN activity and seed size. Two additional mutants, osvin2-2 and osvin2-3 generated by the CRISPR/Cas9 method, exhibited phenotypes similar to those of osvin2-1 in spikelet and seed size, VIN activity, and sugar metabolites. These results clearly demonstrate an important role of OsVIN2 as sink strength modulator that is critical for the maintenance of sucrose flux into developing seed grains.

녹차 종자의 일반성분 (Chemical compositions of the seed of Korean green tea plant(Camellia sinecis L.))

  • 나효환;백순옥;한상빈;복진영
    • Applied Biological Chemistry
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    • 제35권4호
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    • pp.272-275
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    • 1992
  • 한국산 녹차 종자(Camellia sinecis L.)에 함유된 단백질, 탄수화물, 조지방, 조회분 함량은 해바라기(Helianthus annuus)씨나 잇꽃종실(Carthamus tinctorius L.) 등과 대등하였으나, 조사포닌은 12.2%로 참깨(0.29%)나 땅콩(0.63%)보다 월등히 높게 함유하였고, oleic acid를 비롯한 불포화 지방산 조성은 82%였다. Tocopherol은 ${\alpha}-form$$22\;{\mu}g/g$ 보였을 뿐 일반종자보다 현저히 낮은 수준으로 함유하였다.

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굴 수하식 양식에 있어 종묘의 선택이 수익성에 미치는 영향 (The Effects of the Oyster Seed Selection on Profitability of the Oyster Aquaculture Business)

  • 박영병
    • 수산경영론집
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    • 제28권2호
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    • pp.87-105
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    • 1997
  • The objective of this study is to analyze the effects of the oyster seed selection on profitability of the oyster aquaculture business The results of the analysis are as follows ; 1) The comparison of profitability among four different oyster seed applied to the four different scale : 1ha, 3ha, 5ha, and 10ha. The results of the comparison show that, for all scale, the artificial oyster seed is more profitable than the natural oyster seed or the natural oyster seed imported from Japan. 2) There are four important determinant variables of profitability to aquaculture business. In the order of their effect, it is oyster price, quantity of production, labor cost, and seed price. 3) If differences of price between the artificial hardening oyster seed and the natural hardening oyster seed are more less 1,430 won, the former is better. 4) The effect of increasing income of fishermen are estimated about 58.5 billion won or 102 billion won from the artificial oyster seed on the oyster aquaculture.

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QTL Analysis of Soybean Seed Weight Using RAPD and SSR Markers

  • Chung, Jong-Il;Ko, Mi-Suk;Kang, Jin-Ho
    • Plant Resources
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    • 제3권3호
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    • pp.184-193
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    • 2000
  • Soybean [Glycine max (L.) Merr.] seed weight is a important trait in cultivar development. Objective of this study was to identify and confirm quantitative trait loci (QTLs) for seed weight variation in the F2 and F2:3 generations. QTLs for seed weight were identified in F2 and F2:3 generations using interval mapping (MapMaker/QTL) and single-factor analysis of variance (ANOVA). In the F2 plant generation (i.e., F3 seed), three markers, OPL9a, OPM7a, and OPAC12 were significantly (P<0.01) associated with seed weight QTLs. In the F2:3 plant row generation (i.e., F4 seed), five markers, OPA9a, OPG19, OPL9b, OPP11, and Sat_085 were significantly (P<0.01) associated with seed weight QTLs. Two markers, OPL9a and OPL9b were significantly (P<0.05) associated with seed weight QTLs in both generations. Two QTLs on USDA soybean linkage group C1 and R were identified in both F2 and F2:3 generations using interval mapping. The linkage group C1 QTL explained 16% of the variation in seed weight in both generations, and the linkage group R QTL explained 39% and 41% of the variation for F2 and F2:3 generation, respectively. The linkage group C2 QTL identified in F2:3 generation explained 14.9% of variation. Linkage groups C1, C2 and R had previously been identified as harbouring seed size QTLs. The consistency of QTLs across generations and populations indicates that marker-assisted selection is possible in a soybean breeding program.

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블록암호 SEED-192에 대한 취약키 분석 (Analysis for Weak Keys of the Block Cipher SEED-192)

  • 김종성;조기조
    • 한국항행학회논문지
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    • 제15권1호
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    • pp.69-75
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    • 2011
  • 본 논문에서는 블록암호 SEED-192 키 스케줄 취약점을 분석한다. 본 연구 결과에 의하면, SEED-192는 전체 20 라운드 중 16 라운드에 대해, 연관키 렉탱글/부메랑 공격에 대한 취약키가 존재한다. 이는 SEED-192 키 스케줄에 대한 최초의 분석 결과이다.

DNA 표지를 이용한 채종원내 소나무의 교배양식 분석 (Mating System of Japanese Red Pines in Seed Orchard Using DNA Markers)

  • 김영미;홍용표;안지영;박재인
    • 한국자원식물학회지
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    • 제25권1호
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    • pp.63-71
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    • 2012
  • To assess parameters of mating system in seed orchard, such as outcrossing rates, number of potential pollen contributors, and degree of pollen contamination, seeds, produced in '77 plot of the Japanese red pine (Pinus densiflora S et Z) seed orchard at Anmyeon island, were collected in 2007 and analysed by nSSR and cpSSR markers. Estimates of outcrossing rates ranged from 91.2 to 100% (mean 97.7%) on the basis of the analysis of cpSSR haplotypes and from 81.6 to 100% (mean 95.3%) on the basis of the analysis of nSSR genotypes. By cross checking of both DNA markers, seeds, presumed to be products of self pollination on the basis of single marker, were confirmed as outcrossed seeds, which resulted in cumulative outcrossing rates of 98.9%. On the basis of pooled cpSSR haplotype of each seed, the number of pollen contributors and paternal contribution rates were estimated as 14.8 and 0.512, respectively. In conclusion, considering pretty high level of outcrossing rates observed in a seed orchard, good genetic potential of the seeds, produced in '77 plot of the seed orchard of Japanese red pines at Anmyeon island, may be guaranteed. Investigated results from the analysis of mating system of Japanese red pines in a '77 plot of the seed orchard may also be expected to provide useful information for the management and establishment of the seed orchard of the progressive generation.

Maturity Grouping of Korean Soybean Cultivars and Character Relationships According to the Planting Date

  • Ha, Tae-Jeong;Lim, Sea-Gyu;Shin, Seong-Hyu;Choi, Kyung-Jin;Baek, In-Youl;Lee, Sang-Chul;Park, Keum-Yong;Shin, Sang-Ouk
    • 한국작물학회지
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    • 제54권1호
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    • pp.104-118
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    • 2009
  • This study was carried out to classify Korean soybean varieties base on maturity group (MG) and to find character relationships according to planting date for high quality soybean seed production adapted to early season cultivation environment of Miryang. Results of maturity grouping of Korean soybean varieties showed that Keunol (3 cultivars), belonged to Group 0; Seonnok and Danmi in Group II, Shinrok in Group III, Seonyu (17 cultivars), in Group IV, Taekwang (44 cultivars) in Group V, Daewon (25 cultivars) in Group VI, and Kwangdu and Keumdu in Group VII. Agronomic characteristics of 100 soybean varieties were compared based on MG, cultivation year and seeding date. Soybean varieties belonging to the MG $VI{\sim}VII$ showed longer days to flowering and growth period, high lodging density and higher yield. Seed quality analysis revealed that as maturity was delayed, seed weight becomes heavier while seed cracks become abundant. In addition, occurrence of purple seed and phomopsis were higher in MG $0{\sim}III$. Protein content was higher in MG $0{\sim}III$, and isoflavone content was higher as maturity was delayed. On the other hand, lipid content was generally similar across MGs. Correlation analysis of major agronomic characters showed positive relationships between days to flowering and growth days, seed weight and lodging in MG $IV{\sim}V$, seed crack and growth days in MG $0{\sim}III$, seed crack and days to flowering in MG $IV{\sim}V$ and MG $VI{\sim}VII$, seed crack and lodging in MG $IV{\sim}V$ and MG $VI{\sim}VII$, seed crack and seed weight in MG $IV{\sim}V$ and MG $VI{\sim}VII$, purple seed and growth days in MG $IV{\sim}V$, purple seed and seed weight in MG $VI{\sim}VII$, phomopsis and growth days in MG $IV{\sim}V$ and MG $VI{\sim}VII$, and phomopsis and purple seed in MG $IV{\sim}V$ and MG $VI{\sim}VII$. In contrast, a negative relationship was observed between seed weight and lodging in MG $0{\sim}III$. Correlating yield and major characters revealed negative relationships between days to flowering and growth days in MG $0{\sim}III$ and MG $IV{\sim}V$, whereas positive relationships were obtained on MG $VI{\sim}VII$ seeded on April 30. Lodging, seed weight and seed crack were all negatively correlated with yield in the MG $IV{\sim}V$ and MG $VI{\sim}VII$. Soybean cultivars identified as adaptable to early season planting for production of high quality soy curd and fermented soybean paste were Seonyu, Kwangdu, and Soho while those suited for the manufacture of soybean sprouts were Sobaeknanul, Kwangan, Sowon, and Bosuk. Geomjeong 2 chosen as best for mixing with rice.

SEED의 차분전력공격에 대한 강화 방안 (Enhancing the Security of SEED against Differential Power Analysis)

  • 장화선;박상배;김광조
    • 한국정보보호학회:학술대회논문집
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    • 한국정보보호학회 2003년도 동계학술대회
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    • pp.274-280
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    • 2003
  • 최근 부채널 공격으로 스마트카드 같은 장치의 비밀키를 알아낼 수 있음이 알려지면서 많은 알고리즘에 대한 부채널 공격과 대응 방안이 연구되고 있다. DPA는 부채널 공격의 일종으로 암호화 연산 중 발생하는 전력 소모 곡선을 통계적으로 분석하여 키를 알아내는 공격이다. 한편 SEED가 국내 표준 블록 암호 알고리즘으로 널리 사용되고 있으나 SEED에 대한 DPA 연구 결과는 발표된 바가 없는 것 같다. 본 논문에서는 SEED에 DPA를 적용할 수 있음을 보이고 대응방안 예를 제시한다.

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Analysis of Ginsenoside Composition of Ginseng Berry and Seed

  • Ko, Sung-Kwon;Bae, Hye-Min;Cho, Ok-Sun;Im, Byung-Ok;Chung, Sung-Hyun;Lee, Boo-Yong
    • Food Science and Biotechnology
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    • 제17권6호
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    • pp.1379-1382
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    • 2008
  • This study was performed to provide basic information that can be used to differentiate Korean ginseng (Panax ginseng CA. Meyer) berry and seed from American ginseng (Panax quinquefolium L.) seed. Total ginsenoside contents of Korean ginseng berry, Korean ginseng seed, and American ginseng seed were 9.09, 3.30, and 4.06%, respectively. Total ginsenoside content of Korean ginseng berry was about 2.2 to 2.7 times higher than those of Korean ginseng seed and American ginseng seed. Particularly ginsenoside Re content of 4-year cultivated Korean ginseng berry (5.99%) was about 3.6 to 5.4 times higher than that of 4-year cultivated Korean ginseng seed (1.65%) and 4-year cultivated American ginseng seed (1.10%). The contents of total ginsenoside and ginsenoside Re of Korean ginseng berry were about 4.8 and 28 times higher, respectively, than those of 4-year cultivated Korean ginseng root. In general the contents of total ginsenoside and ginsenoside Re of Korean ginseng berry were significantly higher than those of Korean ginseng seed and American ginseng seed.