• Title/Summary/Keyword: secE

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Streptomyces lividans에서 secE 유전자의 클로닝과 염기서열 결정

  • 김순옥;서주원
    • Microbiology and Biotechnology Letters
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    • v.25 no.3
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    • pp.253-257
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    • 1997
  • The secE gene of Streptomyces lividans TK24 was cloned by the polymerase chain reaction method with synthetic oligonucleo- tide primers designed on the basis of the nucleotide sequences of Streptomyces coelicolor secE-nusG-rplK operon. The deduced amino acid sequences of the SecE were highly homologous to those of other known SecE protein, that is 36.8%, 30.4%, 80.0%, and 80.9%, similarity to E. coli, Bacillus subtilis, Streptomyces griseus, Streptomyces virginiae SecE, respectively and exactly same with Streptomyces coelicolor SecE. It means that in spite of evolutionary differences, the genes for protein translocation machinery are highly conserved in eubacteria. The gene organization of secE-nusG-rplK is also similar to that of E. coli, B. subtilis, and streptomycetes.

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Differences of Photosynthetic Ability of Tobacco and Ginseng Leaves in Accordance with Light Intensity (광도에 따른 담배와 인삼엽의 광합성 능력의 차이)

  • Hwang, Jong-Kyu;Hyun, Dong-Yun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.2
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    • pp.211-219
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    • 1989
  • Tobacco and ginseng plants differed in responses to varied light intensities. Tobacco showed high in CO$_2$ uptake and RuBPCase activity at 1900 ${\mu}$ E m/sup-2/ sec$\^$-1/, being high by 3.7 times and 2.7 times than ginseng respectively. Close positive relationships existed between CO$_2$ uptake and RuBPCase activity in tobacco. However, ginseng showed negative correlation. The activity of glycolate oxidase and malate dehydrogenase in tobacco was high at 1900 ${\mu}$ E m/sup-2/ sec$\^$-1/, but those of ginseng was high at 1000 ${\mu}$ E m/sup-2/ sec$\^$-1/. Nitrate reductase activity of tobacco at 1900 ${\mu}$ E m/sup-2/ sec$\^$-1/ was 2 times higher than that at 500 ${\mu}$ E m/sup-2/ sec$\^$-1/, while that of ginseng was no detected in all plots. The content of protein and chlorophyll in tobacco was 2.2 times and 1.5 times higher than in ginseng at the most efficient light intensity. The ratio of chlorophyll a/b in tobacco was low at 500 ${\mu}$ E m/sup-2/ sec$\^$-1/, while that of ginseng was low at 1000 ${\mu}$ E m/sup-2/ sec$\^$-1/. The relationships between protein and chlorophyll was high positive correlation. However, on 5 days after treatment, ginseng showed negative correlation at 500 ${\mu}$ E m/sup-2/ sec$\^$-1/. Tobacco and ginseng showed different leaf soluble protein patterns on SDS-gel electrophoresis. The molecular weights of two major band were 50 KD and 15 KD in both plants. The major bands in tobacco were thinned at 500 ${\mu}$ E m/sup-2/ sec$\^$-1/, while those in ginseng thinned at 1000 ${\mu}$ E m/sup-2/ sec$\^$-1/ from 15days after treatment. Disappeared band was 45 KD at 500 ${\mu}$ E m/sup-2/ sec$\^$-1/ in tobacco, but that of ginseng was 47 KD at 1000 ${\mu}$ E m/sup-2/ sec$\^$-1/.

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A Study of Nerve Conduction Velocity of Normal Adults (정상성인의 신경전도속도에 관한 연구)

  • Choi, Kyoung-Chan;Hah, Jung-Sang;Byun, Yeung-Ju;Park, Choong-Suh;Yang, Chang-Heon
    • Journal of Yeungnam Medical Science
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    • v.6 no.1
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    • pp.151-163
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    • 1989
  • Nerve conduction studies help delineate the extent and distribution of the neural lesion. The nerve conduction was studied on upper(median, ulnar and radial nerves) and lower(personal, posterior tibial and sural nerves) extremities in 83 healthy subjects 23 to 66 years of age. and normal values were established(Table 1). The mean motor terminal latency (TL) were : median. 3.6(${\pm}0.6$)milliseconds ; ulnar. 2.9(${\pm}0.5$) milliseconds ; radial nerve. 2.3(${\pm}0.4$) milliseconds. Mean motor nerve conduction velocity(MNCV) along distal and proximal segments: median. 61.2(${\pm}9.1$) (W-E) and 57.8(${\pm}13.2$) (E-Ax) meters per second ; ulnar. 63.7(${\pm}9.1$) (W-E) and 50.(${\pm}10.0$) meters per second. Mean sensory nerve conduction velocity(SNCV) : median. 34.7(${\pm}6.7$) (F-W), 63.7(${\pm}7.1$) (W-E) and 62.8(${\pm}12.3$) (E-Ax)meters per second ; ulnar. 38.0(${\pm}6.7$)(F-W), 63.4(${\pm}7.5$) (W-E) and 57.0(${\pm}10.1$) (E-Ax)meters per second ; radial, 45.3(${\pm}6.8$) (F-W) and 64.2(${\pm}11.0$) (W-E) meters per second ; sural nerve, 43.4(${\pm}6.1$) meters per second. The amplitudes of action potential and H-reflex were also standardized. Mean H latency was 28.4(${\pm}3.2$) milliseconds. And. the fundamental principles, several factors altering the rate of nerve conduction and clinical application of nerve stimulation techniques were reviewed.

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Effect of Light Conditions on Photoinhibition of Ginseng and Tobacco (광조건이 인삼과 잎담배의 광저해에 미치는 영향)

  • ;Il Hou
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.30 no.2
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    • pp.126-130
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    • 1985
  • Photoinhibition studies were conducted with ginseng (Panax ginseng) grown under shade and tobacco (N. tobaccum cv. Bulgaria) grown under full sunlight. The plants were exposed to light intensity of 580, 1280, 1770, 2580 ${\mu}$E/㎡/sec in normal air for 3.6 9 hours. Light saturation of ginseng was observed at 550 ${\mu}$E/㎡/sec and that of tobacco was at 1600 ${\mu}$E/㎡/sec. Tobacco exposed to high light intensity and long duration of light irradiation didn't show entire reduction in photosynthetic capacity. But in ginseng, 20.3% of photosynthesis was reduced in light intensity of 2,580 ${\mu}$E/㎡/sec during 9 hours. Light response in photosynthesis differed considerably between tobacco and ginseng, and ginseng exposed to high light intensity showed remarkable reduction in photosynthesis. The extent of photoinhibition of ginseng was dependent on the length of exposure to the high light intensity. Stomatal resistance of ginseng seemed not to be changed by photoinhibition but mesophyll resistance was increased.

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The Kinematic Analysis of Handspring Salto Forward Piked (핸드스프링 몸접어 앞공중돌기동작의 운동학적 분석)

  • Kwon, Oh-Seok
    • Korean Journal of Applied Biomechanics
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    • v.17 no.1
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    • pp.145-153
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    • 2007
  • The purpose of this study is to compare and analyze the phase-by-phase elapsed time, the COG, the body joint angle changes and the angular velocities of each phase of Handspring Salto Forward Piked performed by 4 college gymnasts through 3D movement analysis program. 1. The average elapsed time for each phase was .13sec for Phase 1, .18sec for Phase 2, .4sec for Phase 3, and .3sec for Phase 5. The elapsed time for Phase 1 to Phase 3 handspring was .35sec on average and the elapsed time for Phase 4 to Phase 5 handspring salto forward piked was .7sec on average. And so it showed that the whole elapsed time was 1.44sec. 2. The average horizontal changes of COG were 93.2 cm at E1, 138. 5 cm at E2, 215.7 cm at E3, 369.2 cm at E4, 450.7 cm at E5, and 553.1 cm at E6. The average vertical changes of COG were 83.1 cm at E1, 71.3 cm at E2, 78.9 cm at E3, 93.7 cm at E4, 150.8 cm at E5, and 97.2 cm at E6. 3. The average shoulder joint angles at each phase were 131.6 deg at E1, 153.5 deg at E2, 135.4 deg at E3, 113.4 deg at E4, 39.6 deg at E5, and 67.5 deg at E6. And the average hip joint angles at each phase were 82.2 deg at E1, 60 deg at E2, 101.9 deg at E3, 161.2 deg at E4, 97.7 deg at E5, and 167 deg at E6. 4. The average shoulder joint angular velocities at each phase were 130.9deg/s E1, 73.1 deg/s at E2, -133.9 deg/s at E3, -194.4 deg/s at E4, 29.4 deg/s at E5, and -50.1 deg/s at E6. And the average hip joint angular velocities at each phase were -154.7 deg/s E1, -96.5 deg/s at E2, 495.9 deg/s at E3, 281.5 deg/s at E4, 90.3 deg/s at E5, and 181.7 deg/s at E6. The results shows that, as for the performance of handspring salto forward piked, it is important to move in short time and horizontally from the hop step to the point to place the hands on the floor and jump, and to stretch the hip joints as much as possible after the displacement of the hands and to keep the hip joints stretched and high in the vertical position at the takeoff. And it is also important to bend the shoulder joints and the hip joints fast and spin as much as possible after the takeoff, and to decrease the speed of spinning by bending he shoulder joints and the hip joints quickly after the highest point of COG and make a stable landing.

RNA Helicase activity of SecA protein of Escherichia coli

  • Park, Sukyung;Kim, Hyoungman
    • Proceedings of the Korean Biophysical Society Conference
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    • 1996.07a
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    • pp.24-24
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    • 1996
  • SecA protein of E. coli is essential for the translocation of various precursor proteins across the plasma membrane. Along with it, SecA protein interacts with precursor proteins, SecY/E, SecB and is an ATPase which has multiple ATP binding sites. There is little known about the regulation mechanism of the protein translocation machinery. (omitted)

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Prediction of Autoignition Temperature of n-Decane and sec-Butanol Mixture (n-Decane과 sec-Butanol 혼합물의 최소자연발화온도의 예측)

  • Ha, Dong-Myeong
    • Fire Science and Engineering
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    • v.26 no.3
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    • pp.85-90
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    • 2012
  • The autoignition temperature (AIT) of a material is the lowest temperature at which the material will spontaneously ignite. The AIT is important index for the safe handling of flammable liquids which constitute the solvent mixtures. This study measured the AITs of n-Decane+sec-Butanol system by using ASTM E659 apparatus. The AITs of n-Decane and sec-Butanol which constituted binary system were $212^{\circ}C$ and $447^{\circ}C$, respectively. The experimental AITs of n-Decane+sec-Butanol system were a good agreement with the calculated AITs by the proposed equations with a few A.A.D. (average absolute deviation).

A Kinematic Comparative Analysis of Yoko Ukemi(side breakfall) by Each Stage in Judo[ I ] (유도 단계별 측방낙법의 운동학적 변인 비교분석[ I ])

  • Kim, Eui-Hwan;Kim, Sung-Sup
    • Korean Journal of Applied Biomechanics
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    • v.14 no.3
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    • pp.203-218
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    • 2004
  • The purpose of this study was to analyze the comparisons of the kinematical variables when performing Yoko Ukemi(side breakfall) by three Stage in Judo. The subjects were four male judokas who were trainees Y. I. University Squad members and the Yoko Ukemi were filmed by two S-VHS 16mm video cameras(60fields/sec.). The selected times were subject to KWON 3D analysis program and kinematical analysis to compare variables of three Yoko Ukemi. Temporal variables(total time-required : TK, TR by each phase), the body part touched order on the mat and COG variables were computed through video analysis while performing right Yoko Ukemi by three stage. From the data analysis and discussion, the following conclusions were drawn : 1. Temporal variables : total time-required(TR) when performing Yoko Ukemi(side breakfall) by each stage, the first stage(full squat posture: FP : 1.11sec.) showed the shortest time, the next was 3rd(Shizenhontai, straight natural posture: NP : 1.41sec.), and 2nd(Jigohontai, straight defensive posture, DP : 1.42sec.), respectively- 2. TR when performing Yoko Ukemi(side breakfall) by each stage, and phase : the first phase(take of phase, average 0.68sec.) showed the longest time, next was the third phase(ukemi phase, 0.39sec.), and the second phase(air phase, 0.23sec.), respectively. 3. When performing yore Ukemi the body part touched order and TR on the mat : hip(0.94sec.) showed the shortest time, the next was elbow hand(0.97sec.), back(0.98sec.), and shoulder(1.04sec.) order. The hip part touched on the mat the first, but slap the mat in order to alleviate the shock try hand palm and forearm before receiving impact (difference 0.03sec,) 4. Vertical COG variables in each event by each stage : e1(ready position, average 78.33cm) moved the highest, the next was e2(jumping position, 70.14cm), e3(transition position, average 64.00cm), e4(landing position, average 35.99cm), and e5(ukemi position, average 18.32cm) order, gradual decrease respectively. And the difference of COG were showed in initial by each stage, because position fo Yoko Ukemi was difference by each stage in preparation position, but in accordance with executing of Ukemi phase that difference of COG was by decreasing, almost equal displacement in e4(landing) and e5(Ukemi)position finally.

Measurement and Prediction of Autoignition Temperature of n-Butanol and sec-Butanol System (노말부탄올과 2차부탄올 계의 최소자연발화온도의 측정 및 예측)

  • Ha, Dong-Myeong
    • Fire Science and Engineering
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    • v.26 no.5
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    • pp.48-53
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    • 2012
  • The autoignition temperature (AIT) is important index for the safe handling of flammable liquids which constitute the solvent mixtures. This study measured the AITs and ignition delay time for n-Butanol+sec-Butanol system by using ASTM E659 apparatus. The AITs of n-Butanol and sec-Butanol which constituted binary system were $340^{\circ}C$ and $447^{\circ}C$, respectively. The experimental AITs of n-Butanol+sec-Butanol system were a good agreement with the calculated AITs by the proposed equations with a few A.A.D. (average absolute deviation).

A Study on Antiulcer Effects of Opuntia dillenii Haw. on Stomach Ulcer Induced by Water-immersion Stress in Rats (랫드의 스트레스성 위궤양에 대한 Opuntia dillenii Haw.(선인장)의 항궤양작용에 관한 연구)

  • 이후장;이용욱;김정현
    • Journal of Food Hygiene and Safety
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    • v.13 no.1
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    • pp.53-61
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    • 1998
  • This study was performed to investigate the antiulcer effects of Opuntia dillenii Haw. on the stomach ulcer induced by restraint and water-immersion stress in rats. For this experiment, 48 male Sprague-Dawley strain were used. The experimental groups were divided into four: a control (C) and 3 Opuntia dillenii Haw. treatment groups (E-1, E-2, E-3). Each dose of Opuntia dillenii Haw. was 30 mg/kg bw (E-1), 60 mgfKg bw (E-2) and 120 mg/kg bw (E-3). The rats were allocated to each group by 12 and observed for 4 weeks. The results were as following: 1. The stomach surface pH in each group showed no significant difference, but the values of Opuntia dillenii Haw. treatment groups were higher than the value of the control group. 2. The gastric wall mucus was increased in all Opuntia dillenii Haw. treatment groups compared with the control group. Especially in E-1 difference was higher (p<0.05) and in E-2 difference was significantly higher (p<0.01). 3. At shear rate 11.25, 45.0, 90.0, $225\;sec^{-1}$, whole blood viscosity and plasma viscosity were measured. Most of the values of Opuntia dillenii Haw. treatment groups were low compared with that of the control group. At shear rate 90.0, $225\;sec^{-1}$ the values of whole blood viscosity in E-1 were significantly low (p<0.05) and at shear rate 11.25, $45.0\;sec^{-1}$, more significant (p<0.01). At shear rate 11.25, 45.0, 90.0, $225\;sec^{-1}$ the values of whole blood viscosity in E-2 were significantly low (p<0.01). At shear rate $90.0\;sec^{-1}$ the value of plasma viscosity in E-1 was significantly low (p<0.05) and at shear rate 90.0, $225\;sec^{-1}$ the values of plasma viscosity in E-2 we resignificantly low (p<0.01). 4. Less severe ulcers were obsered in Opuntia dillenii Haw. treatment groups than in the control group. Especially E-1 groups tissues had only slight ulcers and necrosis of tissue was not observed in this group. From the results of this study, it can be concluded that the oral administratio-n of Opuntia dillenii Haw. results in protection of stomach ulcer by stimulating the secretion of gastric mucus and improving the gastric mucosal microcirculation.

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