• Journal of Korean Clinical Health Science
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• v.2 no.4
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• pp.247-255
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• 2014

Purpose. This study is based on physical and mental health and physical activity differences in Practice rate each physical activity according to the body to target the 319 people who live in rural areas of 65 and older who have chronic diseases Practice rate activities, general characteristics of chronic diseases, elderly differences in physical activity and investigate the factors that influence. Methods. Physical activity Practice rate was used for descriptive statistics. Each physical activity Practice rate in accordance with the general characteristics of the rural elderly chronic disease were independent T test and One-way ANOVA test. To evaluate the factors influencing the physical activity was the multiple logistic regression analysis. Results. Results of physical activity practice was the most flexibility exercise was walking then, Practice rate each physical activity in accordance with the general characteristics of the elderly are tangible and physical activity radish age, housing type, education level, health insurance part statistically significant were different. Physical health status according to the presence or absence of physical activity showed a significant difference in daily life activities and limited mental health differences were not significant. Factors affecting the physical activity of the elderly with chronic diseases appeared to limit the presence and activities of daily living. Conclusions. As well there is no activity restrictions can be more physically active everyday life well. It is suggested that it is important to approach everyday life, I can help eliminate the factors that independently giving limits on the activities of the elderly with chronic diseases.

• Preventive Nutrition and Food Science
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• v.12 no.1
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• pp.1-6
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• 2007

Colostrum basic proteins (CBP) (MW 1$\sim$30 kDa) were isolated from bovine colostrum using a series of ultrafiltration processes and their effects on osteoblast cell proliferation and bone metabolism were investigated in cell line and animal models. Treatments with CBP (1, 10, 100 $\mu$g/mL) dose-dependently increased cell proliferation of osteoblastic MC3T3 cells. Alkaline phosphatase activity, a marker of osteoblastic phenotype, in the cells was also increased after treatments with CBP in a dose-dependent manner. Significant increases in bone density were observed in femur of ovariectomized rats which were fed a diet with 1% and 10% CBP, compared to rats fed a normal diet. These results suggest that CBP may increase bone mass and density and be useful for the prevention of bone-related diseases.

• Molecules and Cells
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• v.40 no.2
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• pp.117-122
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• 2017

Despite the fact that porcine embryonic stem cells (ESCs) are a practical study tool, in vitro long-term maintenance of these cells is difficult in a two-dimensional (2D) microenvironment using cellular niche or extracellular matrix proteins. However, a three-dimensional (3D) microenvironment, similar to that enclosing the inner cell mass of the blastocyst, may improve in vitro maintenance of self-renewal. Accordingly, as a first step toward constructing a 3D microenvironment optimized to maintain porcine ESC self-renewal, we investigated different culture dimensions for porcine ICM-derived cells to enhance the maintenance of self-renewal. Porcine ICM-derived cells were cultured in agarose-based 3D hydrogel with self-renewal-friendly mechanics and in 2D culture plates with or without feeder cells. Subsequently, the effects of the 3D microenvironment on maintenance of self-renewal were identified by analyzing colony formation and morphology, alkaline phosphatase (AP) activity, and transcriptional and translational regulation of self-renewal-related genes. The 3D microenvironment using a 1.5% (w/v) agarose-based 3D hydrogel resulted in significantly more colonies with stereoscopic morphology, significantly improved AP activity, and increased protein expression of self-renewal-related genes compared to those in the 2D microenvironment. These results demonstrate that self-renewal of porcine ICM-derived cells can be maintained more effectively in a 3D microenvironment than in a 2D microenvironment. These results will help develop novel culture systems for ICM-derived cells derived from diverse species, which will contribute to stimulating basic and applicable studies related to ESCs.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.56-56
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• 2023

The cone of Pinus rigida × Pinus taeda (PRT), a plant in the Pinaceae family, has long been used in traditional medicine to treat hemostasis, bruises, and burns. Previous research has shown that regulating oxidation-reduction reactions in reactive oxygen species can help inhibit melanogenesis, the process of melanin synthesis, which is a common target for addressing hyperpigmentation. Inhibiting tyrosinase is also known to be effective in this regard. Based on these findings, we conducted an investigation into the inhibitory effect of the ethyl acetate fraction of PRT (ERT) on melanogenesis in B16 F10 cells. We know that the expression levels of melanin biosynthesis-related proteins, including tyrosinase, TRP-1, and TRP-2, are regulated by MITF (microphthalmia-associated transcription factor) and cAMP, with cAMP affecting the activity of protein kinase A (PKA). PKA can reduce melanogenesis, and CREB reduces the phosphorylation of melanin-producing enzymes. In addition, the MAPK signaling pathway, composed of ERK, JNK, p38, and other factors, is also known to play a role in the inhibition of melanogenesis in melanocytes. Our immunoblotting results showed that ERT inhibited the expression of melanin production-related proteins (tyrosinase, TRP-1, TRP-2, and MITF) that were significantly increased by a-MSH treatment to promote melanin production. Furthermore, the phosphorylation levels of factors related to cAMP/PKA/CREB and MAPK signaling pathways were significantly reduced without affecting the total form. In conclusion, we believe that treatment with ERT can inhibit melanin synthesis by modulating the phosphorylation of cAMP/PKA/CREB and MAPK signaling pathways at the cellular level. These findings suggest the potential of ERT as a raw material for functional cosmetics and pharmaceuticals, thanks to its antioxidant activity and ability to inhibit melanogenesis. We thought that these findings of ERT as a natural plant resource will inspire further research and development in this area.

• Journal of the Korean Society of Earth Science Education
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• v.8 no.3
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• pp.399-408
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• 2015

This study is to find out that the effects of a creative experience activity program to scientific concepts and self-directed learning skills. This study has been aimed at 2 class 40 students of 4th grade in D metropolitan city A elementary school in 2015, one class 20 students are the research group to apply Scientific research program using creative experience activity, another class 20 students were comparison groups to apply general science classes. The related class section of this study is 4th grade 2 semester of science 4 chapters, 'The Earth and the moon' This section is in fourth grade elementary science curriculum revision in 2009 is a Sections to learn for the first time about astronomical area. Target research group in club activities as part of the creative activities implemented using scientific inquiry and analyzed the results. In addition, in order to better research based on the results of this study as follows. First, the science curriculum in elementary schools, as well as applied research about the creative experience activity classes in other subjects is required. The ongoing research is needed to classes utilizing the characteristics of creative experience activities in several subjects of the elementary school curriculum. Second, Creative experiential learning is only effective when it is done consistently, it is worth studying for long periods of time.

• Journal of The Korean Association For Science Education
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• v.4 no.1
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• pp.44-56
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• 1984

There haw been some courses related to science education for the graduate students, but almost the study for no instructional model and reading material development haw been pursunded in Korea. This study intended to discuss the discipline character of science education research for the systematic survey of concepts, principles and theories related to science education as the first put task, and designed an instructional model of the introductory survey course of science education at graduate level, and then proposed a developmental plan of a reading material for the course teaching aid. The main work of the study was to survey the foreign literature, but the subjective analysis of researcher's courses work during graduate study in abroard and some courses offered to undergraduate and graduate students in Korea by this researcher was quite helpful, even though which was not a strictly designed experimental research. Later it was found that this kind of study was not fitted to the traditional research style but worth while to try for the research in and teaching of "science education" as an area of discipline and professional activity.

• Journal of Korean Elementary Science Education
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• v.34 no.3
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• pp.288-296
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• 2015

This study aimed to analysis of concepts and inquiry activities related to the 'Earth Science' area in the South Korean elementary school textbooks to the current and to seek the improvement of future textbook. For the study, we were collected South Korean elementary school textbooks to the current. After the collection had been made, we were analyzed related to the 'Earth' area in the South Korean elementary school textbooks and were extracted central concepts and inquiry activities. The result of this study: First, there were 'A change in the land', 'Strata and Fossil', 'Volcanoes and Earthquakes', 'Earth and Moon', 'The weather', 'The Solar system and the Star', and 'Seasonal Change' in the central concepts related to the 'Earth' area in the South Korean elementary school textbooks to the current. Second, central concepts were almost the same but the curriculum was changed. Third, inquiry activities also were confirmed to be maintained with little change. This result was believed that it can provide a variety of suggestions at this point in changing the curriculum.

• Preventive Nutrition and Food Science
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• v.14 no.3
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• pp.188-194
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• 2009

In this study, we evaluated the phytochemical compositions of the fruits of the pear cactus, mulberry, and Korean black raspberry to determine how they related to antioxidant capacity. Specifically, the total soluble polyphenol, flavonoid and anthocyanin contents were determined, and the phenolic compounds were evaluated by HPLC. In addition, the antioxidant capacity was evaluated by electron paramagnetic resonance (EPR), SOD-like activity and nitrate scavenging activity. Korean black raspberry was found to have the highest level of phytochemicals, followed by mulberry and pear cactus (p<0.05). Furthermore, EPR revealed that Korean black raspberry extract (100 ${\mu}g$/mL) removed 78.6% of the superoxide radicals in a xanthin-xanthin oxidase. Additionally, when the removal of hydroxyl radicals was evaluated, the extract of pear cactus showed 50% scavenging activity at 1000 ${\mu}g$/mL. Korean black raspberry extract showed the highest SOD-like activity, which was 44% (p<0.05). Finally, the nitrate scavenging activity of both extracts of mulberry and Korean black raspberry were found to be 93%, while that of the pear cactus was 63% at pH 1.2 (p<0.05).

• Journal of Aquaculture
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• v.9 no.2
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• pp.167-168
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• 1996

Experiments were conducted to determine the activities of protease and carbohydrase in growing lensky sturgeon fed with three different diets containing various concentrations of protein and carbohydrate. Neutral pretense activity from growing lensky sturgeon the protein diet (predo-minary, almost $100\%$ protein) was lower then those from fish fed the other diets during the experimental period. The results may indicate that the level of pretense activity is inversely related to the level of protein in the diets.

• Journal of Korean Biological Nursing Science
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• v.18 no.2
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• pp.102-109
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• 2016

Purpose: The purpose of this study was to describe sports injuries among elderly people and to compare physical activity, perceived health status and exercise self-efficacy between elderly people who had experienced a sports injury and elderly people who hadn't. Methods: The sample of this study were 100 elderly in Korea, 51 of whom had had a sports injury. The study used questionnaires to gather data. There were 8 demographic factors, 4 factors related to physical activity, perceived health status and exercise self-efficacy of the subjects. Data were analyzed by frequencies, chi-square test, and t-test. Result: Among the sports injured elders, one occurrence of a sport injury was 86.3%. There were more outdoor than indoor sports injuries of elders (66.7%), and sports injuries of elders occurred more in the winter (82.7%). The major cause of the sports injuries was loss of balance. Ankles were frequently damaged from these injuries. The most significant change after the sports injury was reduction of physical activity (60.8%). There were significant differences between sports injured elderly and non-injured elderly for age (${\chi}^2=2.58$, p=.011) and the amount of physical activity (t=1.96, p=.050), but there was no significant difference in perceived health status and exercise self-efficacy. Conclusion: To prevent sports injuries in the elderly means to maintain a medium level of physical activity and to improve their balance.