• 한국균학회지
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• 제36권1호
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• pp.84-92
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• 2008

장미무당버섯은 담자균문, 주름버섯목, 무당버섯과에 속하며 맛이 좋은 식용 버섯으로 예로부터 암의 치료에 효과가 있다고 알려져 있다. 본 연구에서는 장미무당버섯의 자실체로부터 중성염용액, 열수 및 메탄올을 이용하여 조다당류를 추출하고 ICR mice에 주사하여 항암 및 면역증강 효과를 조사하였다. Sarcoma 180, HepG2, HT-29, NIH3T3 등의 암세포에 대한 독성을 조사한 각각의 암세포는 $10{\sim}2000\;{\mu}g/ml$의 조다당류 농도에서 세포독성을 나타내지 않았다. 각각의 조다당류가 투여된 실험군은 대조군에 비해 수명이 각각 $21.4{\sim}45%$ 연장되었다. 열수로 추출한 조다당류는 B 임파구의 alkaline phosphatase 활성을 $500\;{\mu}g/ml$의 농도에서 대조군에 비해 약 6.8배의 증가시켰다. 중성염추출 조다당류를 50 mg/kg body weight의 농도로 투여한 생쥐의 총 복강 세포 수와 백혈구의 수는 대조군에 비하여 각각 6배 와 2.6배로 증가하였다. 또한 면역에 관련된 장기인 간, 비장 및 흉선의 체중이 대조군에 비하여 소폭 증가된 것으로 조사되었다. 따라서 장미무당버섯의 자실체에서 추출한 조다당류가 Sarcoma 180에 대한 항암작용을 나타내는 것은 이들 조다당류가 Sarcoma 180에 대한 세포독성효과가 아니고 면역을 증강시킨 효과에 의한 것으로 사료된다.

• 한국식품영양과학회지
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• 제26권2호
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• pp.254-260
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• 1997

본 연구에서는 in vitro와 in vivo에서 sarcoma-180 cells과 마우스를 이용하여 김치의 세포독성 효과와 면역계, 특히 대식세포의 탐식능의 활성 증강에 미치는 효과를 중심으로 김치의 항암기작을 연구하였다. 김치가 발효되기 전인 0주(생김치)와 최적의 숙성도를 나타내는 3주 김치$(5^{\circ}C)$를 메탄올, 헥산추출물과 MSF(methanol soluble fraction)로 분리 조제하여 실험에 이용하였다. 김치추출물은 탐식능 증진효과를 나타냈으며, 0주 생김치 보다 3주 발효 김치에서 더욱더 활성이 높았다. 이러한 실험결과를 요약하면 다음과 같다. 1. Sarcoma-180 종양세포에 대한 시료의 직접적인 작용에서는 김치추출물에 의하여 총 세포수가 크게 줄어드는 것을 볼 수 있었다. 3주 발효 김치추출물은 같은 농도에서 0주(생김치) 시료 보다 세포독성작용이 더 높았다. 2. Phagocytic activity는 in vitro와 in vivo에서 대조군에 비해 김치추출물의 모든 시료에서 탐식율이 높게 나타났다. In vitro에서는 대조군이 41.3%에 비해, 3주 김치의 메탄올추출물은 57.3%, 헥산추출물에서는 49.2%를 나타냈고, in vivo에서는 대조군이 24.3%에 비해, 3주 김치의 메탄올추출물은 57.0%, MSF에서는 55.0%였다. 3. In vitro에서는 메탄올추출물에서, in vivo에서는 3주 발효 김치의 메탄올추출물과 MSF에서 대식세포 당 평균 2개 이상의 C. albicans를 탐식하고 있음을 알 수 있었으며 0주(생김치) 보다 3주 김치 추출물에서 더욱더 탐식능 증진효과를 나타냈다.

• 한국식품조리과학회지
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• 제21권5호
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• pp.599-606
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• 2005

The study was carried out to measure the antitumor effect of traditional doenjang (Korean soybean paste) added with Japanese apricot, garlic or ginger. Four kinds of traditional doenjang inhibited significantly the tumor growth in mice injected sarcoma-I80 cells. Especially, traditional doenjang added with ginger (Gi-TD) showed an inhibition of tumor cell activity of 97% by the administration of 1.0 mg/kg methanol extracts. Among Balb/c mouse administered doenjang extracts, the liver weight of mice fed Gi-TD was heavier than that of the group not administered doenjang. However, no difference was found between the control and doenjang administrated groups in weights of body, spleen, kidney and heart. The activity of natural killer (NK) cells was relatively high in mice administrated with the four kinds of doenjang. Particularly, mice administrated with the Gi-TD methanol extracts showed a strong activity of 82.9%. The activity of glutathione S-transferase (GST) in mice administrated with the 4 kinds of doenjang was higher than that of the group not administered with doenjang. In particular, the GST activity was the strongest in the group administrated with Gi-TD. The results suggest that Gi-TD has a strong growth inhibition activity against sarcoma-180 tumor cells.

• Toxicological Research
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• 제27권2호
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• pp.77-83
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• 2011

The objective of this study was to evaluate and compare the antitumor activity of different solvent fractions (ethanol, dichloromethane, ethyl acetate, butanol and water) of the Auricularia auricula-judae 70% ethanol extract on the P388D1 macrophage and sarcoma 180 cells. A dose-dependent antitumor activity of each solvent fraction (from 0.01 mg/ml to 0.3 mg/ml) was shown against both cell types. These cytotoxic effects of all the tested fractions were confirmed on the MTT and SRB assays, without statistical differences each other. $IC_{50}$ value of dichloromethane fraction was 94.2 ${\mu}g/ml$ against sarcoma 180 cells lower than any other solvent fractions. The potent antitumor effect of the dichloromethane (DCM) fraction was also found against solid tumor in BALB/c mice. The splenomegaly and higher splenic index were found in tumor-bearing mice, with the DCM fraction returning to the negative control values. Thus, the results indicated the dichloromethane fraction may have potential ingredients as antitumor candidates.

• 생약학회지
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• 제16권2호
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• pp.99-104
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• 1985

To investigate a possible biological activity of iridoid glucosides, six compounds, aucubin, catalpol, gardenoside, geniposide, rehmannioside and swertiamarin, were studied in relation with their potential influences in RNA and protein biosyntheses in murine tumor cell, sarcoma 180, in vitro. Protein biosynthesis was slightly inhibited by aucubin, gardenoside and swertiamarin. Degree of inhibition of RNA biosynthesis by those iridoid appeared to be more sensitive than that of protein biosynthesis. When aucubin was pretreated with ${\beta}-glucosidase$ to produce its genin form and the sarcoma 180 cells were exposed to this aucubigenin, the protein and RNA biosyntheses in the cells were profoundly inhibited. The results indicate that a biologically active from of iridoid compounds is the hydrolytic products of glycoside, i.e. genin form. It is also suggested that sarcoma 180 cells used in the experiments appear to lack of ${\beta}-glucosidase$, since the inhibitory actions of iridoid glucosides were so slight that those glucosides were not hydrolysed by the enzyme to their genin forms.

• 동아시아식생활학회지
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• 제17권1호
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• pp.43-50
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• 2007

This study investigated the effects of mycelia of Lentinus edodes mushroom-cultured Glycyrrihiza radix(LMG) on cancer cell lines and sarcoma 180(S-180), as well as on human mast cells. In an anti-cancer tests using Hep3B(hepatic cancer cell), MCF-7(breast cancer), and HeLa(uterine cancer) cells, LMG extract exhibited greater anti-proliferation effects than Glycyrrihiza glabra(GG) extract. LMG extract multiplication restraining effects were 60% that of ethanol at 3 mg/mL extract also displayed tumor suppressive effects in mice injected with S-180 cells. The growth-inhibition rates against tumor cells were 56% for LMG and 37% for GG. When LMG was added to human mast cells, the Intensity of RT-PCR products using primers($FC{\varepsilon}RI\;c-kit$) decreased. significantly compared with that of control. These results suggest that Lentinus edodes Mushroom-Cultured Glycyrrhiza glabra has an anti-proliferation effects against cancer cell lines(Hep3B, MCF-7 and HeLa) and S-180 tumors and will be also beneficial in treating allergic reactions.

• 한국균학회지
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• 제34권2호
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• pp.98-104
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• 2006

뽕나무버섯은 담자균문 송이과에 속하며 예로부터 항암 효과를 나타내는 식용버섯으로 알려져 있다. 뽕나무버섯 자실체로부터 중성염용액, 열수 및 메탄올 추출물을 분리하여 생쥐에 대한 항암 및 면역증강효과를 조사하였다. Sarcoma 180 복수암 세포를 주사하고 뽕나무버섯 조다당류를 투여한 실험군의 생쥐가 조다당류를 투여하지 않은 대조군의 생쥐에 비해 생명연장효과가 67.5%로 매우 높게 나타났다. 세포독성 실험결과, NIH3T3와 Sarcoma 180 세포주는 중성염용액, 열수 및 메탄올 추출물 $1000{\mu}g/ml$의 농도에서 배양하였을때 70% 내외의 생존률을 보였으며, 각 농도 구간에서도 세포독성을 나타내지 않았다. 중성염용액 추출물을 투여한 생쥐의 B 임파구 alkaline phosphatase 활성은 대조군에 비하여 약 3.0배 내외의 증가율을 보였으며, 총 복강 세포수도 대조군에 비해 최고 3.5배 가량 증가하였으며, 혈액 중 백혈구의 수도 대조군에 비하여 약 2.5배 증가하였다. 그리고 면역에 관련된 장기인 간, 비장 및 흉선의 체중도 대조군에 비하여 증가한 것을 확인하였다. 따라서 뽕나무버섯의 조다당류 추출물을 투여한 생쥐는 Sarcoma 180에 대한 항암 효과는 면역 활성의 증가에 의한 것으로 사료된다.

• 한국식품영양과학회지
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• 제22권4호
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• pp.405-411
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• 1993

본 연구는 녹황색채소류에서 추출되어 항암효과를 나타내는 활성 물질로 보고되어 있는 phytol과 들미나리 추출물을 sarcoma 180마우스에 주사한 후 적출한 비장세포내 T임프구와 T subset, 그리고 asialo GM1$^{＋}$세포를 정량하여 다음과 같은 결과를 얻었다. 1) 종양 마우스에 phytol 을 투여하였을 때 비장세포내의 T cell과 T-subset은 종양세포이식에 의해서 상승된 치를 더욱 증가시켰다. 그러나 들미나리추출물의 경우는 대동소이하였다. 2) Asialo GM1$^{＋}$세포는 종양마우스에 phytol이나 들미나리추출물을 주사하였을 때 모두 상승하였으며 정상마우스에 phytol 을 투여하였을 때도 대조군에 비하여 상승했지만 들미나리추출물을 작용시켰을 때는 저하되었다. 3) L3T4$^{＋}$/Lyt-2$^{＋}$세포비는 종양마우스에 phytol을 주사하였을 때 감소를 보였지만 정상 마우스에 투여하였을 때는 더욱 크게 낮아졌다. 그러나 들미나리추출물을 정상마우스에 투여 하였을때는 크게 감소하던 것이 종양마우스에 적요 시켰을 때는 증가현상을 보였다. 이상의 결과로 미루어 볼 때 phytol이나 들미나리추출물은 종양마우스에서 작용자 세포인 자연살해세포(natural killer cell)의 활성 인자로서 작용할 가능성이 높을 것으로 사료된다.

• 대한약침학회지
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• 제7권3호
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• pp.77-88
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• 2004

Objectives ; This study was to investigate the anti-cancer effects of herbal acupuncture with distilled fresh ginseng. The herbal acupuncture was injected to Chung-wan($C.V_{12}$) and Wisu($BL_{21}$) of mice that were subjected to Sarcoma-180 adbominal cancer cell and A549 human epithelial lung cancer cells in vitro. Methods : Anti-cancer effects of distilled fresh ginseng herbal acupuncture were tested by measruing Cox, Bcl-2, and Bax by using RT-PCR in A549 human epithelial lung cancer cells in vitro. And four weeks old Balb/c line male mice weighing around $20\;{\pm}\;3g$ were used to measure survival rate and anti-cancer effect to outputs of interleukin-2 and interleukin-4 using flow cytometry, possibility of mRNA menifestation using RT-PCR, and Cox mRNA. The results are as follows. Results : 1. In measuring mRNA menifestation in Cox, Bcl-2, and Bax by using RT-PCR in A549 human epithelial lung cancer cells in vitro, the result showed that fresh ginseng decreased Cox-2 which is directly involved in Inflammation process. 2. Survival rate was measured in an anti-cancer effect experiment against Sarcoma-180 abdorminal cancer. Median survival time of controlled group was 27 days, of experiment group I was 21 days, and of experiment group II was 27 days. Therefore, experiment group I showed -22.2% increase in survival rate and experiment group II showed no difference compare to controlled group. 3. There was no difference between condition group and controlled and experiment group in measuring outputs of interleukin-2 and interleukin 4 by using flow cytometry 4. In measuring outputs of interleukin-2 by using ELISA, there was no significant difference between condition group and controlled group and there was decrease in experiment group II compared to conditioned and controlled group. 5. In measuring cytokine mRNA menifestation by using RT-PCR, experiment group I showed increase of mRNA menifestation in interleukin-2,4 and $interferon-{\gamma}$ and experiment group II showed no significant difference in $interferon-{\gamma}$. Conclusion : According to the results, fresh ginseng herbal-acupuncture took a little effects in cancer. In using distilled fresh ginseng herbal acupuncture has effect on Cox-2 decrease. However, the difference in concentration of fresh ginseng showed no effect on killing cancer cell. It is assumed that inaccurate concentration of herbal acupuncture and fresh ginseng component could be the reason for this result. Therefore, future consideration will be studies on herbal acupuncture concentration.

• Animal cells and systems
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• 제4권3호
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• pp.293-297
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• 2000

The present investigation was performed to elucidate the effect of pretreatment with low dose ultraviolet radiation (UV) and ethyl methanesulfonate (EMS) on cell survival by trypan blue dye exclusion method and plasma membrane glycoconjugates by lectin-cytochemistry in sarcoma 180 (S180) cells. Pretreatment with 2 J/$m^2$ UV or 2 mM EMS increased the percentage of survival of cells subsequently treated with high dose UV (10 or 20 J/$m^2$) or EMS (10 or 20 mM), respectively. Staining intensity of concanavalin A (Con A) of the cells pretreated with 2J/$m^2$ UV or 2 mM EMS and subsequently treated with 10 or 20 mM EMS was stronger than that of the cellstreated with 10 or 20 mM EMS. These results suggest that there is an adaptive response on cell survival to EMS or UV in S180 cells. And the results show a change in mannose-containing glycoconjugates of plasma membrane in S180 cells pretreated with EMS or UV and subsequently treated with EMS.