• BMB Reports
• /
• 제40권1호
• /
• pp.58-64
• /
• 2007

Similar to the other known structures of Bacillus thuringiensis Cry $\delta$-endotoxins, the crystal structure of the 65-kDa activated Cry4Ba toxin comprises three domains which are, from the N- to C-terminus, a bundle of $\alpha$-helices, a three-$\beta$-sheet domain, and a $\beta$-sandwich. To investigate the properties of the C-terminal domain III in isolation from the rest of the toxin, the cloned Cry4Ba-domain III was over-expressed as a 21-kDa soluble protein in Escherichia coli, which cross-reacted with anti-Cry4Ba domain III monoclonal antibody. A highly-purified domain III was obtained in a monomeric form by ion-exchange and size-exclusion FPLC. Circular dichroism spectroscopy indicated that the isolated domain III fragment distinctly exists as a $\beta$-sheet structure, corresponding to the domain III structure embodied in the Cry4Ba crystal structure. In vitro binding analysis via immuno-histochemical assay revealed that the Cry4Ba-domain III protein was able to bind to the apical microvilli of the susceptible Stegomyia aegypti larval midguts, albeit at lower-binding activity when compared with the full-length active toxin. These results demonstrate for the first time that the C-terminal domain III of the Cry4Ba mosquito-larvicidal protein, which can be isolated as a native folded monomer, conceivably participates in toxin-receptor recognition.

• 한국광학회지
• /
• 제24권2호
• /
• pp.86-91
• /
• 2013

본 논문에서는 근접장-분자반응 간의 중첩을 이용한 표면 플라스몬 공명 (SPR) 바이오센서의 측정감도 평가방법을 연구하였다. 전달행렬 방법을 사용하여 다양한 형태의 중첩적분으로 정의된 광학자취 값을 계산하였고, 샌드위치 및 역샌드위치 면역글로뷸린 (IgG) 어세이에 대해서 실험적으로 측정된 수치와 비교하였다. 이론 및 실험적인 결과와의 비교를 통하여 접선 방향의 전기장을 사용한 광학자취의 경우 그 연속성으로 말미암아 가장 높은 상관계수를 얻을 수 있었으며 이때 광학자취와 측정감도 사이에 97% 이상의 높은 상관계수가 존재함을 보았다. 이러한 상관관계는 SPR 바이오센서의 측정 감도에 관한 메커니즘을 분명하게 설명하며, 분자 스케일 감도를 가지는 SPR 바이오센서 개발에 기여하게 될 것이다.

• Steel and Composite Structures
• /
• 제36권1호
• /
• pp.1-16
• /
• 2020

In steel-concrete composite beams, to improve the cracking resistance of the concrete slab in the hogging moment region, a new type of connector in the interface, named uplift-restricted and slip-permitted screw-type (URSP-S) connector has been proposed. This paper focuses on the behavior of steel-concrete composite beams with URSP-S connectors. A total of three beam specimens including a simply supported beam with URSP-S connectors and two continuous composite beams with different connectors arrangements were designed and tested. More specifically, one continuous composite beam was equipped with URSP-S connectors in negative moment region and traditional shear studs in other regions. For comparison, the other one was designed with only traditional shear studs. The failure modes, crack evolution process, ultimate capacities, strain responses at different locations as well as the interface slip of the three tested specimens were measured and evaluated in-depth. Based on the experimental study, the research findings indicate that the larger slip deformation is allowed while using URSP-S connectors. Meanwhile, the tensile stress reduces and the cracking resistance of the concrete slab improves accordingly. In addition, the overall stiffness and strength of the composite beam become slightly lower than those of the composite beam using traditional shear studs. Moreover, the arrangement suggestion of URSP-S connectors in the composite beam is discussed in this paper for its practical design and application.

• Structural Engineering and Mechanics
• /
• 제50권5호
• /
• pp.635-652
• /
• 2014

Creep and shrinkage behaviour of an ultra lightweight cement composite (ULCC) up to 450 days was evaluated in comparison with those of a normal weight aggregate concrete (NWAC) and a lightweight aggregate concrete (LWAC) with similar 28-day compressive strength. The ULCC is characterized by low density < 1500 $kg/m^3$ and high compressive strength about 60 MPa. Autogenous shrinkage increased rapidly in the ULCC at early-age and almost 95% occurred prior to the start of creep test at 28 days. Hence, majority of shrinkage of the ULCC during creep test was drying shrinkage. Total shrinkage of the ULCC during the 450-day creep test was the lowest compared to the NWAC and LWAC. However, corresponding total creep in the ULCC was the highest with high proportion attributed to basic creep (${\geq}$ ~90%) and limited drying creep. The high creep of the ULCC is likely due to its low elastic modulus. Specific creep of the ULCC was similar to that of the NWAC, but more than 80% higher than the LWAC. Creep coefficient of the ULCC was about 47% lower than that of the NWAC but about 18% higher than that of the LWAC. Among five creep models evaluated which tend to over-estimate the creep coefficient of the ULCC, EC2 model gives acceptable prediction within +25% deviations. The EC2 model may be used as a first approximate for the creep of ULCC in the designs of steel-concrete composites or sandwich structures in the absence of other relevant creep data.

• Steel and Composite Structures
• /
• 제42권4호
• /
• pp.501-511
• /
• 2022

Earthquake Resistant Design Philosophy seeks (a) no damage, (b) no significant structural damage, and (c) significant structural damage but no collapse of normal buildings, under minor, moderate and severe levels of earthquake shaking, respectively. A procedure is proposed for seismic design of low-rise reinforced concrete special moment frame buildings, which is consistent with this philosophy; buildings are designed to be ductile through appropriate sizing and reinforcement detailing, such that they resist severe level of earthquake shaking without collapse. Nonlinear analyses of study buildings are used to determine quantitatively (a) ranges of design parameters required to assure the required deformability in normal buildings to resist the severe level of earthquake shaking, (b) four specific limit states that represent the start of different structural damage states, and (c) levels of minor and moderate earthquake shakings stated in the philosophy along with an extreme level of earthquake shaking associated with the structural damage state of no collapse. The four limits of structural damage states and the three levels of earthquake shaking identified are shown to be consistent with the performance-based design guidelines available in literature. Finally, nonlinear analyses results are used to confirm the efficacy of the proposed procedure.

• Steel and Composite Structures
• /
• 제43권2호
• /
• pp.165-183
• /
• 2022

Most transportation departments have recognized and developed procedures to address the ever-increasing weights of trucks traveling on bridges in a service today. Transportation agencies also recognize the issues with overheight vehicles' collisions with bridges, but few stakeholders have definitive countermeasures. Bridges are becoming more vulnerable to collisions from overheight vehicles. The exact response under lateral impact force is difficult to predict. In this paper, nonlinear impact analysis shows that the degree of deformation recorded through the modeling of the unprotected vehicle-girder model provides realistic results compared to the observation from the US-61 bridge overheight vehicle impact. The predicted displacements are 0.229 m, 0.161 m, and 0.271 m in the girder bottom flange (lateral), bottom flange (vertical), and web (lateral) deformations, respectively, due to a truck traveling at 112.65 km/h. With such large deformations, the integrity of an impacted bridge becomes jeopardized, which in most cases requires closing the bridge for safety reasons and a need for rehabilitation. We proposed different sacrificial cushion systems to dissipate the energy of an overheight vehicle impact. The goal was to design and tune a suitable energy absorbing system that can protect the bridge and possibly reduce stresses in the overheight vehicle, minimizing the consequences of an impact. A material representing a Sorbothane high impact rubber was chosen and modeled in ANSYS. Out of three sacrificial schemes, a sandwich system is the best in protecting both the bridge and the overheight vehicle. The mitigation system reduced the lateral deflection in the bottom flange by 89%. The system decreased the stresses in the bridge girder and the top portion of the vehicle by 82% and 25%, respectively. The results reveal the capability of the proposed sacrificial system as an effective mitigation system.

• 한국재료학회지
• /
• 제33권5호
• /
• pp.214-221
• /
• 2023

As a filler metal for lowering the melting point of Ag, many alloy metal candidates have emerged, such as cadmium, with zinc, manganese, nickel, and titanium as active metals. However, since cadmium is known to be harmful to the human body, Cd-free filler metals are now mainly used. Still, no study has been conducted comparing the characteristics of joints prepared with and without cadmium. In addition, studies have yet to be conducted comparing the typical characteristics of brazing filler metals with special structures, and the joint characteristics of brazing filler metals with available frames. In this study, the characteristics of junctions of silver-based intercalation metals were compared based on the type of filler metal additives, using a special structure, a filler metal sandwich structure, to protect the internal base metal. The general filler metal was compared using the structure, and the thickness of the filler metal according to the thickness was reached. A comparison of the characteristics of the junction was conducted to identify the characteristics of an intersection of silver-based brazing filler metal and the effect on joint strength. Each filler metal's collective tensile strength was measured, and the relationship between joint characteristics and tensile joint strength was explored. The junction was estimated through micro strength measurement, contact angle measurement with the base metal when the filler metal was melted, XRD image observation, composition analysis for each phase through SEM-EDS, and microstructure phase acquisition.

• Composites Research
• /
• 제36권3호
• /
• pp.230-240
• /
• 2023

전개형 반사판 안테나는 단위 구조물 형태의 반사판이 접힌 상태로 수납되어 발사체에 탑재된 후, 운용궤도에 도달 및 전개되어 임무를 수행하는 위성체이다. 전개형 반사판 안테나는 수납 부피를 줄일 수 있어 발사체의 제한적 수납공간에 대형 우주 구조물을 탑재시킬 수 있으며, 경량소재를 적용할 경우 발사 및 운용 성능 향상에 용이한 장점이 있다. 본 논문에서는 전개형 반사판 안테나를 구성하는 주반사판에 대해 강성 및 강도 등의 구조적 분석을 통해 초기 개념설계를 수행하였다. 탄소섬유 복합재 및 허니콤 코어를 적용하여 경량 복합재 주반사판을 설계하였으며, 적층 패턴 및 형상을 설계 변수로 운용조건에 적합한 주반사판 설계안을 도출하였다. 이후 모드(Modal analysis), 준정적(Quasi-static), 열 구배(Thermal gradient) 및 동적(Dynamic) 거동에 대한 상세 구조해석을 수행하여 경량 복합재 반사판 안테나의 성능을 분석하였다

• Structural Engineering and Mechanics
• /
• 제87권4호
• /
• pp.297-304
• /
• 2023

Targeted introduction of explosion-resisting and energy-absorbing materials and optimization of explosion-resisting composite structural styles in underground engineering are the most important measures for modern engineering protection. They could also improve the survivability of underground engineering in wartime. In order to test explosion-resisting and energy-absorbing effects of high-performance equal-sized-aggregate (HPESA) concrete, the explosive loading tests were conducted on HPESA concrete composite plates by field simple explosion craters. Time-history curves of the explosion pressure at the interfaces were obtained under six conditions with different explosion ranges and different thicknesses of the HPESA concrete plate. Test results show that under the same explosion range, composite plate structures with different thicknesses of the HPESA concrete plate differ significantly in terms of the wave-absorbing ability. Under the three thicknesses in the tests, the wave-absorbing ability is enhanced with the growing thickness and the maximum pressure attenuation index reaches 83.4%. The energy attenuation coefficient of the HPESA concrete plate under different conditions was regressively fitted. The natural logarithm relations between the interlayer plate thickness and the energy attenuation coefficient under the two explosion ranges were attained.

• Composites Research
• /
• 제30권6호
• /
• pp.356-364
• /
• 2017

복합재 격자 구조는 동일한 무게를 갖는 다른 구조에 비해 더 큰 하중을 견딜 수 있다는 장점으로 인해 다양한 분야에 적용이 시도되고 있다. 최근, 국내에서도 복합재 격자 구조 제작을 위한 기술 개발이 이루어지고 있으며 이에 복합재 격자 구조를 빠르고 정밀하게 검사할 수 있는 비파괴검사 기술의 개발 역시 필요하게 되었다. 본 논문에서는 초음파전파 영상화 시스템들을 활용하여 복합재 격자 구조에 빠르고 정밀한 비파괴검사를 하기 위한 연구를 수행하였다. 레이저 펄스에코 초음파전파 영상화 시스템을 통해 스킨에 쌓여 있는 복합재 격자 구조의 내부 리브 구조를 관찰할 수 있었고 접착분리를 검출할 수 있는 가능성을 확인하였다. 또한 검사시간을 줄이기 위해 주파수 영역을 최적화 하기 위한 밴드 디바이더를 개발 적용하였으며, 검사 결과의 질을 향상시키기 위해 곡률 보상 알고리즘을 개발하였다. 유도파 초음파전파 영상화 시스템으로는 리브 구조에 있는 층간분리 결함을 확인할 수 있었으며, 다중 소스 초음파전파영상을 통해 검사 영역을 확대시켰고 가변시간창 진폭 이미지 알고리즘을 통해 결함을 강조시킬 수 있도록 했다. 이와 같은 결과들을 통해 격자구조에 최적화 된 초음파전파 영상화 시스템의 지속적인 개발이 이뤄지면 복합재 격자 구조의 대량생산에 이은 고속 정밀 비파괴검사가 이뤄질 수 있을 것으로 판단된다.