• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.277.1-277.1
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• 2013

Molecular diagnostics consists of three processes, which are a sample pretreatment, a nucleic acid amplification, and an amplicon detection. Among three components, sample pretreatment is an important process in that it can increase the limit of detection by purifying nucleic acid in biological sample from contaminants that may interfere with the downstream genetic analysis such as nucleic acid amplification and detection. To achieve point-of-care virus detection system, the sample pretreatment process needs to be simple, rapid, and automatic. However, the commercial RNA extraction kits such as Rneasy (Qiagen) or MagnaPure (Roche) kit are highly labor-intensive and time-consuming due to numerous manual steps, and so it is not adequate for the on-site sample preparation. Herein, we have developed a rotary microfluidic system to extract and purify the RNA without necessity of external mechanical syringe pumps to allow flow control using microfluidic technology. We designed three reservoirs for sample, washing buffer, and elution buffer which were connected with different dimensional microfluidic channels. By controlling RPM, we could dispense a RNA sample solution, a washing buffer, and an elution buffer successively, so that the RNA was captured in the sol-gel solid phase, purified, and eluted in the downstream. Such a novel rotary sample preparation system eliminates some complicated hardwares and human intervention providing the opportunity to construct a fully integrated genetic analysis microsystem.

• Geomechanics and Engineering
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• v.14 no.2
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• pp.131-139
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• 2018

The preparation of repeatable and uniformly reconstituted soil specimens up to the specified conditions is an essential requirement for the laboratory tests. In this study for large samples replication, the simultaneous usage of the traveling pluviation and curtain raining technique is used to develop a new method, called the curtain travelling pluviator (CTP). This simple and cost effective system is based on the air pluviation approach, whilst reducing the sample production time, can reproduce uniform samples with relative densities ranging from 25% to 96%. In order to investigate the resulting suitability and uniformity from the proposed method, a series of tests is performed. The effect of curtain traveling velocity, curtain width, drop height, and flow rate on the parameters of the sample is thoroughly investigated. Increase in the curtain velocity and drop height leads to the increase in relative density for the sand specimen. Increase in curtain width typically resulted in the reduction of relative density. Test results reveal that the terminal drop height for the sand specimen in this study is more than 500 mm. Relative density contour lines are presented that can be utilized in optimizing the drop height and curtain width parameters. Sample uniformity in the vertical and horizontal orientation is investigated through the sampling containers. Increasing relative density tends to result in the higher sample repeatability and uniformity.

• 한국전자현미경학회:학술대회논문집
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• 1999.05a
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• pp.53-56
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• 1999

• 한국가시화정보학회:학술대회논문집
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• 2002.04a
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• pp.85-99
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• 2002

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.3
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• pp.955-960
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• 2012

In this study, pure Polyurethane and commonly used flame retardants $Al(OH)_3$ and $Mg(OH)_2$, the add-in sample and Cloisite Na+, Cloisite 15A added sample of flammability and mechanical properties are compared. Sodium silicate and a mixture of polyurethane that the lightweight filler Vermiculite, Perlite addition of flame retardant and mechanical properties of the sample was confirmed. As a result, the flame retardant additive in the Vermiculite and Perlite in a flame-retardant grade sample except sample were identified. Tensile strength is degrade accord to the filler amount. But, Sodium silicate in the case of the sample add-in, other than confirmed that the sample strength increases.

• Fibers and Polymers
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• v.3 no.3
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• pp.97-102
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• 2002

A series of waterbome poly(urethane-urea) anionomers were prepared from isophorone diisocyanate (IPDI), polycaprolactone diol (PCL), dimethylol propionic acid (DMPA), ethylene diamine (EDA), and triethylamine (TEA), NaOH, or Cu($(COOCH_3)_2$) as neutralizing agent. This study was performed to decide the effect of neutralizing agent type on the particle size viscosity, hydrogen bonding index, adhesive strength, antistaticity, antibacterial and mechanical properties. The particle size of the dispersions decreased in the following order: TEA based samples (T-sample), NaOH based samples (N-sample), and Cu($(COOCH_3)_2$) based sample (C-sample). The viscosity of the dispersions increased in the order of C-sample, N-sample, and T-sample. Metal salt based film samples Of and C-sample) had much higher antistaticity than TEA based sample. By infrared spectroscopy, it was found that the hydrogen bonding index (or fraction) of samples decreased in the order of T-sam-pie, N-sample, and C-sample. The adhesive strength and tensile modulus/strength decreased in the order of T-sample, N-sam-pie, and C-sample. The C-sample had strong antibacterial halo, however, T- and N-samples did not

• Fisheries and Aquatic Sciences
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• v.1 no.2
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• pp.187-191
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• 1998

Chemotherapy can not be applied for the control of fish viral diseases because viruses depend on host machinery for their replication. Although new control strategies including vaccination are under development, avoidance of virus introduction by rapid and correct diagnosis is the best way of fish viral disease control. Although observation of virus particles with an electron microscope is an easy method for virus detection, it take a few days for the sample preparation. In order to shorten the sample preparation time, microwave radiation was applied in the procedure. With this method, 15 seconds was enough for fixation of virus infected fish samples or cultured cells inoculated with infectious hematopoietic necrosis virus, which takes 2-4 hours with routine methods. Also four minutes was enough for polymerization of embedding resin which takes 24-48 hours with routine methods. Samples prepared with microwave were good enough for direct electron microscopic observation and immunogold labeling assay.

• The Journal of the Petrological Society of Korea
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• v.13 no.3
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• pp.126-132
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• 2004

This study provides detailed sample preparation methods for subgrain zircon analyses, and a simple mineral separation technique which overflows light mineral grains out of beaker using the running water from faucet. Excluding separation steps using of the Wilfley table and heavy liquid, this technique is faster and more efficient than conventional one, and remarkably suitable for collecting small amount of zircon for subgrain analyses.

• Journal of Magnetics
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• v.19 no.2
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• pp.101-105
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• 2014

In this work, Zinc ferrite nanoparticles have been prepared by various methods, conventional (ZC), mechanochemical processing (ZM) and Sol-Gel (ZS) method, to compare their structural and magnetic properties. The cation distribution obtained from XRD shows the degrees of inversions are 4%, 14.8%, and 16.4% from the normal $ZnFe_2O_4$ structure. Fourier transform infrared spectroscopy (FT-IR) confirms changes in cation distribution of $ZnFe_2O_4$ fabricated by sol-gel and mechanochemical processing. The $^{57}Fe$ M$\ddot{o}$ssbauer spectra of the samples were recorded at room temperature. The spectra exhibit a line broadening. The magnetic properties of the samples were studied by vibration sample magnetometer (VSM) at room temperature and the results show that the sample ZM has ferrimagnetic behaviour.

• Bulletin of the Korean Chemical Society
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• v.35 no.1
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• pp.69-75
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• 2014

Accurate analysis of fly ash particles is not trivial because of complex nature in physical and chemical properties. SPLITT fractionation (SF) was employed to fractionate the fly ash particles into subpopulations in large quantities. Then the SF-fractions were analyzed by the steric mode of sedimentation field-flow fractionation (Sd/StFFF) for size analysis. The SF-fractions were also analyzed by ICP-OES. The results showed that the fly ash is mainly composed of Fe, Ca, Mg and Mn. No particular trends were observed between the particle size and the concentrations of Fe, Ca, Mg, while Mn, Cu and Zn were in higher concentrations in smaller particles. Sample preparation procedures were established, where the fly ash particles were sieved to remove large contaminants, and then washed with acetone to remove organics on the surface of particles. The sample preparation and analysis methods developed in this study could be applied to other environmental particles.