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BOND STRENGTH AND MICROLEAKAGE IN RESIN BONDING TO TOOTH STRUCTURE (치질접착에서 접착강도와 변연누출)

  • Kim, Jin-Hee;Park, Jeong-Won;Park, Jin-Hoon;Kim, Sung-Kyo
    • Restorative Dentistry and Endodontics
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    • v.24 no.4
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    • pp.570-577
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    • 1999
  • Intuitively, higher bond strengths should result in less leakage. However, the relationship between bond strengths and microleakage value is complex and not clearly understood. The purpose of this study was to evaluate the relationship between tensile bond strengths and microleakage values in the same restorations to understand the behavior of resin bonding to tooth structure. One-hundred and twenty enamel or dentin specimens from freshly extracted bovine mandibular incisors were used. The specimen was treated with 32% phosphoric acid for 15 seconds and rinsed for 20 seconds. the teeth were divided into four groups by means of wet bonding technique or dry bonding. One-Step$^{TM}$ adhesive were applied to the specimen. The specimens were immersed in 2% methylene blue solution for 7 days, and tensile bond strength and microleakage were measured. The results were as follows: 1. Significant negative correlation was found between bond strengths and micro leakage values. Hence, higher bond strengths seem to be associated with lower microleakage, and vice versa (r=-0 50, p<0.05). 2. The Enamel/Wet group showed significantly higher bond strength than Enamel/Dry one, and Dentin/Wet group showed higher strength than Dentin/Dry one (p<0.05). 3. Microleakage was significantly less ill wet bonding than in dry one at dentin (p<0.05), however, there was no significant difference between wet and dry bonding at enamel (p>0.05).

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The influence of occlusal loads on stress distribution of cervical composite resin restorations: A three-dimensional finite element study (교합력이 치경부 복합레진 수복물의 응력분포에 미치는 영향에 관한 3차원 유한요소법적 연구)

  • Park, Chan-Seok;Hur, Bock;Kim, Hyeon-Cheol;Kim, Kwang-Hoon;Son, Kwon;Park, Jeong-Kil
    • Restorative Dentistry and Endodontics
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    • v.33 no.3
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    • pp.246-257
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    • 2008
  • The purpose of this study was to investigate the influence of various occlusal loading sites and directions on the stress distribution of the cervical composite resin restorations of maxillary second premolar, using 3 dimensional (3D) finite element (FE) analysis. Extracted maxillary second premolar was scanned serially with Micro-CT (SkyScan1072; SkyScan, Aartselaar, Belgium). The 3D images were processed by 3D-DOCTOR (Able Software Co., Lexington, MA, USA). HyperMesh (Altair Engineering, Inc., Troy, USA) and ANSYS (Swanson Analysis Systems, Inc., Houston, USA) was used to mesh and analyze 3D FE model. Notch shaped cavity was filled with hybrid (Z100, 3M Dental Products, St. Paul, MN, USA) or flowable resin (Tetric Flow, Vivadent Ets., FL-9494-Schaan, Liechtenstein) and each restoration was simulated with adhesive layer thickness ($40{\mu}m$). A static load of 200 N was applied on the three points of the buccal incline of the palatal cusp and oriented in $20^{\circ}$ increments, from vertical (long axis of the tooth) to oblique $40^{\circ}$ direction towards the buccal. The maximum principal stresses in the occlusal and cervical cavosurface margin and vertical section of buccal surfaces of notch-shaped class V cavity were analyzed using ANSYS. As the angle of loading direction increased, tensile stress increased. Loading site had little effect on it. Under same loading condition, Tetric Flow showed relatively lower stress than Z100 overall, except both point angles. Loading direction and the elastic modulus of restorative material seem to be important factor on the cervical restoration.

Package Atmosphere and Quality as Affected by Modified Atmosphere Conditions of Persimmon (Diospyros kaki. cv. Fuyu) Fruits ('부유' 단감의 MA포장 조건에 따른 포장기체조성 및 과실의 품질 변화)

  • Ahn, Gwang-Hwan;Song, Won-Doo;Park, Doo-Sang;Lee, Yeon;Lee, Dong-Sun;Choi, Seong-Jin
    • Korean Journal of Food Science and Technology
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    • v.33 no.2
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    • pp.200-204
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    • 2001
  • Persimmon (Diospyros kaki. cv. Fuyu) fruits were packaged under different conditions, and then stored at $0^{\circ}C$ for 21 weeks. The tried packages were heat-sealed bag of one fruit, heat-sealed bag of five fruits, and tie-sealed bag of five fruits, which used films of three different thickness (0.03 ㎜, 0.05 ㎜ and 0.06 ㎜). Throughout the storage, package free volume, package atmosphere and quality were measured. Package free volume decreased with time with higher rate for heat-sealed bags, in which close contact between fruit and the film was eventually reached in longer storage. However, tie-sealed bags maintained the levels of stabilized free volume. The rate of free volume decrease was faster with thinner film and larger bag size for the packs sealed by the same method. Package atmosphere covered $O_2$ concentration of $1.1{\sim}17.1%$ and $CO_2$ concentration of $1.1{\sim}8.3%$, $O_2$ concentration decreased during storage, whereas $CO_2$ increased. Thinner film package created the internal atmosphere of higher $O_2$ and lower $CO_2$ concentrations. Tie-sealed bags of 5 fruits in the films of 0.05 mm and 0.06 mm thickness maintained the equilibrated package atmosphere of $1.1{\sim}3.0%\;O_2$ and $4.0{\sim}5.5%\;CO_2$, which preserved the fruits best in terms of firmness and less physiological changes of flesh browning and peel browning. At five fruit heat-sealed bag in the films of 0.06 mm thickness with $CO_2\;8.3%$, flesh browning occurred at a high rate, whereas all treatments in the film of 0.03 mm thickness with high $O_2$ and relatively low $CO_2$ contents, flesh and peel browning rates were high.

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Anti-carcinogenic Effects of Korean Mistletoe Extract and Lectin in Experimental Hepatocarcinogenesis (실험적 간암모델에서 한국산 겨우살이(Mistletoe)추출물 및 렉틴의 발암 억제효과 탐색)

  • 김미정;김정희
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.4
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    • pp.697-702
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    • 2001
  • This study was done to investigate effects of Korean mistletoe extract and lectin on serum GOT, GPT and $\alpha$-L-fucosidase activities and the preneoplastic lesion in chemically induced rat hepatocarcinogenesis. To attain the above objectives weanling Sprangue-Dawley male rats were fed modified AIN-76 diets containing 10% corn oil for 9 weeks. One week after feeding rats were intraperitonealy injected twice with a dose of diethylnitrosamine (DEN, 50 mg/kg body weight(BW)) and were provided 0.05% phenobarbita (PB) with drinking water from one week after DEN treatment until the end of experiment. For the same period as PB treatment, rats were injected mistletoe extract (10 $\mu\textrm{g}$/kg BW European mistletoe, 10 $\mu\textrm{g}$/kg BW and 100 $\mu\textrm{g}$/kg BW Korean mistletoe) and lectin(1 ng/kg BW, 10 ng/kg BW) twice a week. At the end of 9th week rats were sacrificed and the formation of hepatic glutthione S-transferase placental form positive (GST-P+) foci serum GOT, GPT and $\alpha$-L-fucosidase activities were determined. By treatment of mistletoe extract or lectin there were no significant effects on serum GOP, GPT and $\alpha$-L-fucosidase activities whereas those activities showed a tendency to increase by DEN treatment. The formation of GST-P+ foci was significantly decreased by mistletoe extract or lectin treatment especially in group of 100$\mu\textrm{g}$/kg BW Korean mistletoe. These results suggest that Korean mistletoe extract and lectin have a possibility to inhibit hepatocarcinogenesis of animals.

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Cytotoxicity and Multidrug -Resistance Reversing Activity of Extracts from Gamma-Irradiated Coix Zachryma-jobi L. var. ma-yuen Stapf Seed (감마선 조사된 율무종자의 세포독성 및 다제내성 극복활성)

  • Cha, Young-Ju;Lee, Sook-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.5
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    • pp.613-618
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    • 2005
  • This study was carried out to examine the effects of gamma irradiation on the cytotoxicity and multidrug-resistance reversing activity of methanol extracts from Coix lachryma-jobi L. var. me-yuen Stapf seed. The seed was irradiated with doses of 1, 4, 8, 16, 32 and 64 Gy of the gamma radiation, and then extracted by methanol. The extracts were examined for cytotoxicity on the human cancer cell lines, MCF-7 (human breast adenocarcinoma pleural effusion), Calu-6 (human pulmonary carcinoma) and SNU-601 (human gastric carcinoma) cells, and investigated for multidrug-resistance reversing activity using drug sensitive AML-2/WT and multidrug-resistant AML-2/D100 cells. The growth inhibitory activity of irradiated seed extracts on human cancer cell lines was higher than that of the control. In the case of Calu-6 cell line, the effect of cytotoxicity was observed in the extracts of 4, 8 and 16 Gy. $IC_{50}$ value in the MCF-7 cell line was measured in the only 8 Gy extract. And in the SNU-601 cell line as Calu-6, the effect of cytotoxicity was observed in the extracts of 4, 8 and 16 Gy. But the extracts of gamma-irradiated seed over 32 Gy showed little growth inhibitory effect against human cancer cell lines. In this result, 8 Gy extract had significant growth inhibitory in all human cancer cell lines $(Calu-6:\;633\;{\mu}g/mL,\;MCF-7:\;653\;{\mu}g/mL\;and\;SNU-601:\;683\;{\mu}g/mL)$. The extracts of 4, 8 and 16 Gy strongly potentiated vincristine cytotoxicity in AML-2/D100 cells. The reversal fold (RF) of 4, 8 and 16 Gy extracts was 1.7, 1.8 and 1.6, respectively. But their cytotoxicities to both sensitive AML-2/WT and resistant AML-2/D100 cells were in the same order of magnitude. These results indicate that the above samples would contain some principles which have cytotoxicity and multidrug-resistance reversing activity. Irradiation technology can be applied to promote physiological activities of medicinal plant seeds.

Effects of Pearl Barley on the Growth and Development of Albino Rats (율무쌀의 경구투여(經口投與)가 백서(白鼠)의 성장(成長)에 미치는 영향(影響))

  • Cho, Soo-Yeul;Park, Myeung-Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.6 no.1
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    • pp.41-47
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    • 1977
  • This study was designed to evaluate the nutritive value of pearl barley. Forty males and the same number of females of albino rats, the Leuise strain, weighing from 52.1g to 59.1g were divided into eight experimental groups, five males and females were composed of one group. A group: casein 15%+starch 75%+oil 8%+vitamin mixture 1%+salt mixture 1% B group: pearl barley 98%+vitamin mixture 1%+salt mixture 1% C group: rice 98%+vitamin mixture 1%+salt mixture 1% D group: barley 98%+vitamin mixture 1%+salt mixture 1% E group: rice 49%+pearl barley 49%+vitamin mixture 1%+salt mixture 1% F group: rice 73.5%+pearl barley 24.5%+vitamin mixture 1% +salt mixture 1% G group: barley 49%+pearl barley 49%+vitamin mixture 1% +salt mixture 1% H group: barley 73.5%+Pearl barley 24.5%+vitamin mixture 1%+salt mixture 1% The rats were kept in an individual cage and were fed 8 different diets for 7 weeks and fed by ad-libitum feeding method. The results of this study were eluciated as followings. 1. B group was significantly lower in weight gain than the other groups. 2. Polished pearl barley showed higher digestion rate in protein and carbohydrate than brown pearl barley but lower in fat. 3. Liver weight was obserbed significant differences between B group and C group. but no significant differences between the others. 4. B group was significantly lower in the total protein content of liver and A/G ratio in plasma than other groups. 5. B group was also the lowest in the hematocrit and hemoglobin contents among the experimental groups. 6. Free amino acid composition in plasma was similar to those in diet.

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Carrageenan Content and its Chemical Composition of Eucheuma cottonii from the Coast of Philippine (Philippine산(産) Eucheuma cottonii의 carrageenan 함량(含星)과 그 성상(性狀)에 대하여)

  • Kim, Soon-Seon;Kim, Seun-Bong;Kim, In-Su;Jeung, Mi-Hee;Park, Yeung-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.8 no.1
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    • pp.63-70
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    • 1979
  • One species of Rhodophyceae namely Eucheuma cottonii from the coast of Philippine was analyzed with respect to the content of carrageenan and such chemical characteristics as the content of sulphate and 3,6-anhydro-D-galactose and its solubility in potassium chloride solution. In addition, the same chemical properties were tested in the fractions separated by the different concentrations of potassium chloride. In comparison of the results of carrageenan in Eucheuma cottonii samples from Philippine and Chondrus ocellatus samples from Korea, carrageenan content in Eucheuma cottonii was higher than that of Chondrus ocellatus. Both samples showed more than forty-five percent carrageenan content. The Eucheuma cottonii carrageenan showed a higher 3,6-anhydro-D-galactose than the Chondrus ocellatus carrageenan. The sulphate content was higher in Chondrus ocellatus than Eucheuma cottonii. In fractionation of carrageenan by the solubility methods using potassium chloride solution, the yield of Eucheuma cottonii was highest in fraction I, fraction III was next and fraction II was the lowest.

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Factors affecting the survival of out planted Cryptomeria and Japanese cypress Seedlings (I) -Especially on the drying and water soaking duration- (삼나무(杉), 편백나무 산출묘(山出苗)의 활착(活着) 영향인자(影響因子(I) -건조(乾燥)(실내(室內), 실외(室外), OED green) 및 침지(浸漬)-)

  • Lee, Jyung Seuk;Oh, Kwang In
    • Journal of Korean Society of Forest Science
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    • v.44 no.1
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    • pp.26-35
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    • 1979
  • Factors affecting the survival of out planted Cryptomena and Japaneses Cypress seedlings. (I) - Especially on the drying and water soaking duration ­ There are often the cases of poor survival percentage for the economic forest establishments of Cryplomeria japonica D. Don. and Chamaecyparis obtusa End. This study was carried out to investigate the survival factors of both 1-1 seedlings and Cryptomena cutting Seedlings by room drying conditions, day drying conditions, OED green water soaking treatment and drying duration at the forest nursery of Chonnam National University from February to September. 1979. The results are as follows: 1. From 11 a.m. to 3 p.m. fluctuation in then moisture contents(MC) rapidly decreased and M.C. fluctuation in indoor until 5 p.m. showed the same results with the amount of an hour fluctuation in outdoor. 2. M.C. fluctuations between OED green and non-treatment of these seedlings were silghtly different. 3. The survival percentage of OED green and non-treatment of seedlings were highly significant in the room drying conditions and the day drying conditions respectively The effects of water soaking treatment on rooting was negligible. The survival percentage of seedlings of OED green was higher than that of non-treatment. 4. Although survival percentage of Japanese Cypress was almost in accord with Cryptomena, the power of resistance to drying was lower m Japanese Cypress than m Cryptomeria. 5. Compared with Cryptomena seedlings the survival percentage of Cryptomeria cutting seedlings was higher and not influenced at all in case of being drying for two hours; but it was highly significant in interactions between the drying time and the water soaking time. 6. When Cryptomena and Japanese Cypress seedling become drying in indoor for four days, the survival percentage remarkably reduced after two days and also was highly significant among numbers of drying days.

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Appropriateness Evaluation of Plug Seedling Cultivation of Rehmannia glutinosa (지황의 플러그 육묘재배 적정성 평가)

  • Lee, Sang-Hoon;Lee, Yoon-Jeong;Oh, Myeong-Won;Lee, So-Hee;Koo, Sung-Cheol;Hur, Mok;Lee, Woo-Moon;Chang, Jae-Ki;Kim, Ei-Hyun;Han, Jong-Won
    • Korean Journal of Plant Resources
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    • v.33 no.2
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    • pp.73-79
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    • 2020
  • This study was conducted to evaluate the appropriateness cultivation of R. glutinosa by plug seedling. Cultivation by direct seeding (DS) and plug seedling (PS) of R. glutinosa was conducted in each of the Pyeongchang and Eumseong areas. As a result, locally, for the Eumseong area, aerial parts in leaf length, leaf width and number of leaves per plant were better than for the Pyeongchang area. In the cultivation method, leaf width and number of leaves per plant were better with PS cultivation than with DS cultivation. Locally, rhizome yield of underground parts in the Eumseong area was better than that in the Pyeongchang area, but the rhizome yield with DS cultivation in the Eumseong area and PS cultivation in the Pyeongchang area were statistically the same. The fresh rhizome yield for DS in Eumseong, PS in Eumseong, DS in Pyeongchang and PS in Pyeongchang was 1,253 kg/10a, 1,376 kg/10a, 923 kg/10a and 1,256 kg/10a, respectively. Finally, the catalpol content for DS in Eumseong, PS in Eumseong, DS in Pyeongchang and PS in Pyeongchang was 3.67%, 2.03%, 2.96% and 1.60%, respectively. From these results, it can be seen that the R. glutinosa PS cultivation method can increase the rhizome yield.

Phytophagous Insect Fauna of Dicotyledoneae(Tracheophyta : Angiospermae) Weeds (쌍자엽 식물아강(관속식물문 : 피자식물강)의 잡초가해 곤충상)

  • ;;Patrick J.Shea
    • Korean journal of applied entomology
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    • v.31 no.4
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    • pp.496-508
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    • 1992
  • Phytophagous insects associated with Dicotyledoneae weeds and host specificities in the field populations were investigated for the survey of biological control agents of weeds in Korea. Fifty four weed species in 39 genera were collected during the survey. The most insects were collected from Polygonales by 24 species in 22 genera and followed by Urticales and Centrospermales by 17 species of 17 genera. The insects collected in the other weed orders were ranged from 1 to 12 species. Out of 17 insect species collected in Urticales, Baris sp. damaged the leaves of Hamulus japonicus in Cannabinaceae as scattered holeshape and showed host specificity. In Polygonaceae, Rumex japonicus and R. crispus were severely damaged by Aphis rumicis and Gastrophysa atrocyanea. G. atrocyanea leaf beetle had host specificity on R. japonicus and ate all the leaves except veins. The leaf beetle, Lypesthes japonicus was a potential biological control agent by feeding leaves of Persicaria spp .. And Lixus spp. were also often collected from Persicaria spp .. Liothrips vaneeckei was first collected from weed, P. modosa. P. senticosa was damaged by unidentified geometrid moth larvae and P. perfoiliata by Miarus atricolor snout beetle. Cassida piperata damaged leaves of Chenopodium album of Centrospermales and showed host specificity. In a soybean field, C. album and Amaranthus mangostanus were severely damaged by Spodoptera litura larvae which were eating soybean leaves. This phenomenon indicates that the presence of weed in cultivated land influences the outbreak of insect pests. Altica oleracea leaf beetle was frequently collected from Oenothera spp. of Onagraceae in Myrtales. Aphis gossyphi was outbroken on Solanum nigrum and Phylliodes brettinghami leaf beetle was first recorded on the same plant. Leaf beetles, Longitarsus scutellais and Hemipyxis plagioderoides were first collected from Plantago asiatica of Plantaginaceae in Plantaginales. They showed host specificities in the fields. The hemipterans were collected from many weeds during the survey and their roles on weeds should be investigated. A tractomorpha bedeli was also collected from many kinds of weeds in forest areas.

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