• Title/Summary/Keyword: salts concentration

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Effect of Fungal Elicitor and Heavy Metals on the Production of Flavonol Glycosides in Cell Cultures of Ginkgo biloba

  • KIM, MIN SOO;CHUL KIM;DO HYUN JO;YEON WOO RYU
    • Journal of Microbiology and Biotechnology
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    • v.9 no.5
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    • pp.661-667
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    • 1999
  • The effect of fungal elicitor and heavy metal salts on the production of flavonol glycosides in cell cultures of Ginkgo biloba was investigated. Among the fungi tested, Trichoderma longibrachiatum ATCC 52326 was found to be the most efficient in the production of flavonol glycosides. Kaempferol production from the elicited callus increased ten-fold as compared to the unelicited callus, while quercetin concentration of elicited cells was nine-fold higher than that of uneliceited cells in suspension cultures. The maximum quercetin concentration of 0.362㎎/l was obtained in 1.25㎎/l of the homogenate elicitor. Among the heavy metal salts tested, CuSO₄ showed a significant effect on quercetin accumulation, reaching to the concentration of 0.526 ㎎/l. Quercetin concentration increased to a maximum of l2-fold in response to CuSO₄ treatment as compared to that of untreated cells. The phenylalanine ammonia-lyase (PAL) activity and flavonol glycosides production simultaneously increased for 5 days of culture after fungal elicitor feeding, and their contents showed the same proportional patterns during the culture period. In contrast, PAL activity of cell cultures treated with CuSO₄ was almost constant during the culture period, although quercetin production increased remarkably.

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한.러 생명공학 공동 심포지움 참관기

  • 이상기
    • The Microorganisms and Industry
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    • v.20 no.3
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    • pp.70-73
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    • 1994
  • Effects of 13 organic compounds including glucose, fructose, xylose, glutamate, succinate, malate, glycine, lactate, acetate, pyruvate, citrate, formate and cis-aconitate on the oxidation of thiosulfate and the availability of these compounds as the substrate for the respiration by Thiobacillus ocncretivorus, which is known to be an obligated autotroph, were studied. Malate nad glycine at 0.5 per cent concentration nearly doubled the thiosulfate oxidation compared to the control. No other organic substances enhanced the thiosulfate oxidation compared to the control. No other organic substances enhanced the thiosulfate oxidation. Moreover, some 30 to 40 per cent decrease was recorded by fructose, sulfate-salts medium, some 30 to 40 per cent decrease was recorded by fructose, citrate, xylose, malate, flucose, glutamate and succinate. No respiration could occur when formate and pyruvate were supplied as the substrate for respiration. But it was obvious that flucose, fructose, xylose, glutamate, malate, citrate and succinate could be used as the substrate for respiration to some extent, regarding the fact that some increase in respiration rates could be recorded compared to the result from the salts medium, where neither thiosulfate nor orgnic compounds were added. Thus, it was postulated that this organism could possibly be converted into mixotroph or hetrotroph if appropriate conditions could be prepared.

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Preparation of WC Powders by SHS Process in the Presence of Alkali Salts (자전연소합성법에서의 알칼리염을 이용한 WC 분말의 제조)

  • Won, Hyung-Il;Nersisyan, Hayk;Won, Chang-Whan
    • Journal of the Korean Ceramic Society
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    • v.44 no.3 s.298
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    • pp.152-156
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    • 2007
  • Tungsten carbide powder was synthesized by SHS (self-propagating high-temperature synthesis). Except $WO_{3}$, each concentration of raw material ($WO_{3},\;Mg,\;NaCl,\;Na_{2}CO_{3},\;C$) was investigated. Final product was characterized by XRD and SEM. X-ray data demonstrated that the $NaCl+Na_{2}CO_{3}$ combined mixture has superiority in the WC formation process. Single phase and submicrometer WC powder was synthesized at the temperature below $1600^{\circ}C$. The role of sodium salts in combustion process was discussed, and chemical mechanism of WC formation was proposed. WC powder prepared by salt-assisted combustion synthesis has a size $0.2{\sim}3\;{\mu}m$ and low agglomeration degree.

Preparation of Nano AgBr Particles by Microemulsions (마이크로에멀젼을 이용한 AgBr 나노입자의 제조)

  • Jeong, Noh-Hee;Kim, Hong-Soo
    • Journal of the Korean Applied Science and Technology
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    • v.21 no.1
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    • pp.17-23
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    • 2004
  • Silver bromide particles from 50 to 200${\AA}$ in diameter are prepared by mixing two microemulsions contaning the precursor salts $AgNO_3$ and KBr. The microemulsions are composed of AOT(bis(2-ethylhexyl) sodium sulfosuccinate), n-heptane and water. The particle diameters are measured on photomicrographs obtained by transmission electron microscopy. The size of the particles is generally larger than that of the water cores. The influence of both the concentration of precursor salts in the water cores of the microemulsoin and the size of these water cores on the size of the particles has been studied.

Root Induction and Propagation of Sedum takesimense Nakai Using Leaf Cutting Method

  • Cheong, Eun Ju
    • Journal of Forest and Environmental Science
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    • v.34 no.1
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    • pp.50-52
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    • 2018
  • The effect of basal nutrients concentrations and exogenous auxin for root induction from leaves of Sedum takesimense were investigated for mass-propagation. Root induction rates were significantly different from the concentrations of basal salts but not influenced by supplemented IBA in the medium. The lowest concentration of MS basal salts (1/10) was most effective to induce roots from leaves followed 1/5 MS, and 1/2 and full strength MS medium. Supplement of IBA $10{\mu}M$ in the medium did not improve the root induction that resulted no differences compare to the hormone free media. Rooted leaves were transplanted in soil and survived in greenhouse.

Studies on the Isopolytungstic Acid and Isopolytungstates (이소폴리 텅스텐酸 및 그의 鹽에 關한 硏究)

  • Bong-Kul Kim
    • Journal of the Korean Chemical Society
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    • v.13 no.4
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    • pp.317-324
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    • 1969
  • Isopolytungstic acid and it's salts, which are formed in sodium tungstate solutions, when acidified with perchloric acid, have been investigated by cryoscopic method. The results obtained are summarized as follows; It was not possible to measure the molecular weight of sodium isopolytungstates which are salthydrates, by means of cryoscopic method. Regardless of the concentration of solution, paratungstate and acid hexatungstate are formed at $H^+/WO_4^{2-}$ ratio of 1.15 and 1.50. It has been shown that isopolytungstic acid and it's salts are also formed at $H^+/WO_4^{2-}$ ratio of 1.30 and 1.90 and in highly concentrated solutions at 1.40 and 1.65.

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Biodegration of Formaldehyde-Releasing Preservatives

  • Park, Won-Jae;Yang, Seung-Kak
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.11 no.1
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    • pp.1-12
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    • 1985
  • A strain of Pseudomonas aeruginosa was isolated from the spoiled product and its characteristics on various formaldehyde-releasing preservatives were investigated. This strain, P. aeruginosa FR, could utilize 1.0% of imidazolidinyl urea and 0.2% of DMDM hydantoin as a sole carbon and nitrogen source in the minimal salts medium. With the growth of the strain in minimal salts medium containing imidazolidinyl urea, formic acid was initially accumulated according to the decrease of formaldehyde concentration. It was suggested that formaldehyde dehydrogenase was involved in this oxidation process and could catalyze formaldehyde, imidazolidinyl urea, DMDM hydantoin and quaternium-15, but not bronopol. MICs of this strain to each preservation were 0.03% in formaldehyde, 1.0% in imidazolidinyl urea, 0.2% in DMDM hydantoin, 0.2% in quaternium-15 and 0.1% of EDTA-2Na. But the MICs were diminished about ten times when 0.01% of EDTA-2Na was added to the preservative systems. In actual challenge test, the eyeliner and the pack which contained paraben and imidazolidinyl urea were not able to be protected from this strain, but when 0.05% EDTA-2Na was added the products were sufficiently preserved.

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Studies on the Shrinkage of Silk Yarn by Neutral Salts (견의 중성염류에 의한 염축에 관한 연구)

  • 이용우;이광길
    • Journal of Sericultural and Entomological Science
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    • v.33 no.2
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    • pp.87-92
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    • 1991
  • The shrinkages and physical properties of silk yarn were studied on the effect of treatment concentration, time and temperature with various neutral salts. The degummed silk yarn was shrunk about 35 percent by highly concentrated solution of Ca(NO3)2 on conditions of S.G.(special gravity) 1.45 at 9$0^{\circ}C$ or S. G. 1.46 at 8$0^{\circ}C$. About 40 percent of silk yarn was contracted in length by the treatment of CaCl2 solution on conditions of S. G. 1.33 at 9$0^{\circ}C$ for 5 mins. or at 85$^{\circ}C$ for 10 mins. By treating the concentrated solution of LiBr the silk yarn showed about 40 percent of shrinkage on a conditions of S. G. 1.38, $25^{\circ}C$ 24hrs. The physical properties of salt-treated silk yarn could be significantly changed with shrinkage variations. There were some differences in shrinkages between different type of salts. However, it was observed that generally the tenacity was decreased and breaking elongation increased as the shrinkage increased. The bulkiness of salt-treated silk yarn was increased by 110 to 120 percent compared with untreated yarn.

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Effect of Combined Use of Sun-dried Salt and Monosodium Glutamate on Sodium Concentration in Vegetable Rice Porridge and Bean-sprout Soup (채소죽과 콩나물국의 천일염과 MSG 사용에 따른 나트륨 함량 변화)

  • Sung, Dongeun;Park, Jae Young;Han, Jiseok;Park, Yooyoung;Cho, Mi Sook;Oh, Sangsuk
    • Journal of the Korean Society of Food Culture
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    • v.32 no.1
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    • pp.52-57
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    • 2017
  • The feasibility of reduction of sodium intake using sun-dried salt and monosodium glutamate (MSG) was studied. Preference test was performed to evaluate the sensory properties of bean-sprout soup and vegetable rice porridge soup. Sun-dried salt and MSG might be a partial substitute for refined salt. There was a significant difference in salt taste strength between sun-dried salt and refined salt. Sun-dried salts 0.45% with MSG 0.07% resulted in the highest taste preference compared to that of sun-dried salts 0.60% without MSG in bean-sprout soup, which resulted in 23.9% reduction of sodium intake. Sun-dried salts 0.38% with MSG 0.04% resulted in the highest taste preference compared to sun-dried salts 0.53% without MSG in vegetable rice porridge soup, which resulted in 25.4% reduction of sodium intake. There seemed to be a synergistic effect on reduced usage of sodium salt when MSG was used in vegetable rice porridge and bean-sprout soup with sun-dried salt.

Inverse HPLC approach for the evaluation of repulsive interaction between ionic solutes and a membrane polymer

  • Kiso, Yoshiaki;Kamimoto, Yuki;Hosogi, Katsuya;Jung, Yong-Jun
    • Membrane and Water Treatment
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    • v.6 no.2
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    • pp.127-139
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    • 2015
  • Rejection of ionic solutes by reverse osmosis (RO) and nanofiltration (NF) membranes is controlled mainly by electrochemical interaction as well as pore size, but it is very difficult to directly evaluate such electrochemical interaction. In this work, we used an inverse HPLC method to investigate the interaction between ionic solutes and poly (m- phenylenediaminetrimesoyl) (PPT), a polymer similar to the skin layer of polyamide RO and NF membranes. Silica gel particles coated with PPT were used as the stationary phase, and aqueous solutions of the ionic solutes were used as the mobile phase. Chromatographs obtained for the ionic solutes showed features typical of exclusion chromatographs: the ionic solutes were eluted faster than water (mobile phase), and the exclusion intensity of the ionic solute decreased with increasing solute concentration, asymptotically approaching a minimum value. The charge density of PPT was estimated to be ca. 0.007 mol/L. On the basis of minimum exclusion intensity, the exclusion distances between a salt and neutralized PPT was examined, and the following average values were obtained: 0.49 nm for 1:1 salts, 0.57 nm for 2:1 salts, 0.60 nm for 1:2 salts, and 0.66 nm for 2:2 salts. However, $NaAsO_2$ and $H_3BO_3$, which are dissolved at neutral pH in their undissociated forms, were not excluded.