• Journal of Nutrition and Health
• /
• v.50 no.1
• /
• pp.32-40
• /
• 2017

Purpose: Obesogenic environments in children, in particular excessive intake of sodium, generate hypertension, which is a major risk factor for chronic diseases. Methods: In all, 725 children, 379 boys and 373 girls, aged 8~9 years were recruited from seven elementary schools in Kuro-ku, Seoul. To evaluate whether or not obesity risk was modulated by salt-sensitive genes, Solute Carrier Familiy 12 member 3 (SLC12A3) was used as the target. After children were assigned into obese (BMI > 85 percentile) or non-obese groups, anthropometry, blood biochemistry, and dietary intakes were measured according to the genotypes GG (wild) or GA+AA (hetero+mutant). Results: Without gender differences, high TG and low HDLc were detected in the obese group compared to the non-obese group. Regardless of obesity, weight gain and blood pressure (BP) increased in the SLC12A3 GA+AA genotype rather than in the GG type. HDLc was associated with obesity risk without genotype difference. Odd ratios for risk of obesity were 15.57 (95% CI 2.192~110.654), 22.84 (95% CI 1.565~333.469), and 9.32 (95%CI 1.262~68.817) in boys and girls with GA+AA genotypes as sodium intake increased above 4,000 mg/day. Dietary calcium, sodium, folate, and vit C were associated with obesity risk according to gender or genotype differences. Since high folate intake reduced obesity risk in only boys with GG type. Risk for overweight and obesity increased in boys with GA+AA genotypes and dietary habits with high sodium and cholesterol and low folate. Conclusion: The A allele of SLC12A3 rs11643718 was sensitive to development of obesity in children as sodium intake increased.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.65 no.4
• /
• pp.406-415
• /
• 2020

Maize (Zea mays L.) is one of the most valuable agricultural crops and is grown under a wide spectrum of environmental conditions. However, maize is moderately sensitive to salt stress, and soil salinity is a serious threat to its production worldwide. In this study, we used ethyl methane sulfonate (EMS) to generate salt-tolerant silage maize mutants. We screened salt-tolerant lines from 203 M₃ mutant populations by evaluating the morphological phenotype after salt stress treatment and selected the 140ES91 line. The 140ES91 mutant showed improved plant growth as well as higher proline content and leaf photosynthetic capacity compared with those of wild-type plants under salt stress conditions. Using whole-genome re-sequencing analysis, 1,103 single nucleotide polymorphisms and 71 insertions or deletions were identified as common variants between KS140 and 140ES91 in comparison with the reference genome B73. Furthermore, the expression patterns of three genes, which are involved in salt stress responses, were increased in the 140ES91 mutant under salt stress. Taken together, the mutant line identified in our study could be used as an improved breeding material for transferring salt tolerance traits in maize varieties.

• Journal of Environmental Health Sciences
• /
• v.21 no.3
• /
• pp.96-101
• /
• 1995

A selective and highly sensitive spectrophotometric measurements have been developed for the determination of trace mercury(II) with thiosulfate and PAR. Hg(II)-thiosulfate complex was extracted into high molecular alkylamine such as quaternary ammonium salt and back extracted into 1 M-HCl solution. The Hg(II)-PAR complex has maximum absorbance at 499 nm and obeys Beer's law in the range of 0.04~1.0 $\mu g/mL$ of mercury(II). The molar absorptivity and Sandell's sensitivity are $6.27\times 10^4 L.mole^{-1} cm^{-1}$ and $3.2\times 10^{-3}g/cm^2$ respectively.

• Macromolecular Research
• /
• v.17 no.4
• /
• pp.227-231
• /
• 2009

New polyelectrolytes derived from poly(amide-sulfone)s and 1,5-dibromopentane were simultaneously fabricated on the electrode by the crosslinking reaction. The substrate was pretreated with a bromoalkyl-containing, silane-coupling agent to anchor the humidity-sensitive membrane to the substrate through the covalent bond. When the resistance dependence on the relative humidity of the crosslinked poly(amide-sulfone)s was measured, the resistance varied by three orders of magnitude between 20%RH and 90%RH, which was the required RH range for a humidity sensor operating at ambient humidity. Their water durability, long-term stabilities under various environments, hysteresis and response and recovery times were measured and evaluated as a humidity-sensing membrane.

• Proceedings of the Korean Society of Plant Biotechnology Conference
• /
• 2005.04a
• /
• pp.58-58
• /
• 2005

• Korean Journal Plant Pathology
• /
• v.14 no.6
• /
• pp.636-641
• /
• 1998

Digoxigenin (DIG) was used to prepare nucleic acid probe for the detection of RNA of potato leafroll virus (PLRV) in the potato leaf extracts. The 0.6 kb coat protein (CP) gene cDNA of PLRV in plasmid pSPT 18 vector was labeled with digoxigenin by in vitro run-off transcription and then used for cRNA probe. In the several buffers tested for increase the total RNA extraction efficiency AMES buffer was the most suitable for this detection method. The RNA extracts from potato leaves shown symptoms of PLRV were dot blotted onto nylon membrane and hybridized with labeled RNA probes. After hybridization, labeled RNA bound to PLRV RNA on membrane was detected with anti-digoxigenin alkaline phosphatase. 5-bromo-4-chloro-3-indolyl-phosphate/nitroblue tetrazolium (NBT) salt and CSPD were used as substrate for colorimetric and film exposure detection, respectively. These detection methods were very sensitive allowing for detection of 1/32 diluted total RNA extract from 100 mg leaf tissue.

• Nuclear Engineering and Technology
• /
• v.54 no.6
• /
• pp.2004-2010
• /
• 2022

Quantification of sensitive material is of vital importance when it comes to the movement of nuclear fuel throughout its life cycle. Within the electrorefiner vessel of electrochemical separation facilities, the task of quantifying plutonium by neutron analysis is especially challenging due to it being in a constant mixture with curium. It is for this reason that current neutron multiplicity methods would prove ineffective as a safeguards measure. An alternative means of plutonium verification is investigated that utilizes the (𝛼,n) signature that comes as a result of the eutectic salt within the electrorefiner. This is done by utilizing the multiplicity variable a and breaking it down into its constituent components: spontaneous fission neutrons and (𝛼,n) yield. From there, the (𝛼,n) signature is related to the plutonium content of the fuel.

• Journal of Life Science
• /
• v.18 no.2
• /
• pp.154-161
• /
• 2008

The physiological and photochemical responses of rice seedling to NaCl stress were investigated through measuring leaf relative water content (RWC), root water uptake and chlorophyll fluorescence. When plants were exposed to increased salinity stress, the visual symptoms of injury were significant at ${\geq}$500 mM NaCl concentration for 4 and 5 day stress periods. The differences in Fv/Fm between control treatment and plants treated with 500 mM and 1,000 mM NaCl were evident after 5 day and 4 day, respectively, whereas in root water uptake its effect was observed at 500 mM and 1,000 mM NaCl at 2 day of salt-stressed periods. Leaf RWC in salt-stressed plants decreased gradually with increasing salinity in exogenous solution and duration of salt stress, and these decrease showed leaf RWC of 58-68% atduration over 2 day stress of 1,000 mM NaCl treatment and 88% at 1 day stress. NaCl stress led to a significant inhibition of the light-induced greening in etiolated rice plants, especially in 4 and 5 day salt-stressed plants, which linearly decreased with NaCl concentration ($R^2$=0.812 and 0.918, respectively). The effects of NaCl stress in rice seedlings indicate that water uptake in root is more sensitive to increasing NaCl concentration and stress duration than Fv /Fm in leaves compared with the same NaCl concentration.

• Korean Journal of Microbiology
• /
• v.47 no.3
• /
• pp.231-240
• /
• 2011

The objective of this study was to evaluate the acid and bile tolerance, bile salt hydrolase (BSH) activity, and cholesterol assimilation ability of lactic acid bacteria isolated from mustard leaf kimchi. MLK11, MLK22, MLK27, MLK41, and MLK67 were relatively acid- and bile-tolerant strains, with more than $10^5$ CFU/ml after incubation in simulated gastric juice and intestinal fluid, while MLK53 was the most sensitive strain to acid and bile. Strains MLK22 and MLK67 deconjugated the highest level of sodium glycocholate with more than 3.5 mM of cholic acid released, while deconjugation was lowest by strains MLK13 and MLK41 which released only 1.35 mM and 1.16 mM, respectively. Specially, strains MLK22 and MLK67 showed higher deconjugation of sodium glycocholate compared to sodium taurocholate and conjugated bile mixture. Although strains MLK22 and MLK67 exhibited maximal BSH activity at the stationary phase, MLK22 had somewhat higher total BSH activity compared to MLK67 towards both sodium glycocholate and sodium taurocholate. Meanwhile, cholesterol removal varied among tested strains (p<0.05) and ranged from 5.22 to 39.16 ${\mu}g$/ml. Especially, MLK67 strain assimilated the highest level of cholesterol in media supplemented with 0.3% oxgall, cholic acid, and taurocholic acid (p<0.05). According to physiological and biological characteristics, pattern of carbohydrate fermentation, and 16S rDNA sequence, strain MLK67 that may be considered as probiotic strain due to acid and bile tolerance and cholesterol-lowering effects was identified as Pediococcus pentosaceus MLK67.

• Journal of Korean Society of Environmental Engineers
• /
• v.32 no.3
• /
• pp.256-263
• /
• 2010

The study was performed to evaluate the effects of chloride concentrations on the ultimate aerobic biodegradability and to use the result as the fundamental data for sea food wastewater treatment. When the organic removal efficiency by chloride concentrations (1,400~18,000 mg/L) was evaluated, microbes adapted to the saline at ${\leq}$ 6,000 mg/L of chloride but treatment efficiency was not improved at ${\geq}$ 12,000 mg/L of chloride because of delayed reaction time. Functional coefficient $Y_I$ of non-biodegradable soluble organic and inert material production coefficient Yp by microbe metabolism increased as chloride concentrations increased. Soluble organic matter ratio by chloride concentration (0~18,000 mg/L) was 10.8~13.1%, inert material production efficiency by microbes metabolism was evaluated as 7.0~24.6%. $NH_3$-N removal efficiencies were 96.2, 96.5, 90.2 and 90.3% using original wastewater HRT 18 hr, 6,000 mg/L chloride concentration HRT 22 hr, 12,000 mg/L chloride concentration HRT 30 hr, and 18,000 mg/L chloride concentration HRT 45 hr, respectively. Nitrification process was more sensitive to salt concentration than organic matter removal to salt concentration. Under ${\geq}$ 6,000 mg/L chloride concentration, conversion rate from $NO_s$-N to $NO_2$-N was low.