• Title/Summary/Keyword: salt analysis

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Nutrient Value of Saltwort (Salicornia herbacea L.) as Feed for Ruminants

  • Ishikawa, N.;Shimizu, K.;Koizumi, T.;Shimizu, T.;Enishi, O.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.7
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    • pp.998-1001
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    • 2002
  • Saltwort (Salicornia herbacea L.), a kind of halophytes in Japan, is under investigation as a feed source for livestock as well as potential oilseed crop in salt marshes and salt fields. The present experiment was undertaken to analyze the nutritive value of saltwort as feed for ruminants. To determine the apparent digestibility and nutritive value of saltwort, five Japanese native goats were fed the diets consisting of alfalfa hay cubes with 15 or 20% (n=3 and n=2, respectively) inclusion levels of saltwort in the total diet on a DM basis. All the animals were randomly offered alfalfa hay cubes as a base diet or a mixed diet of alfalfa hay cubes and saltwort at maintenance level, thereafter, alternative feed (a base or mixed diet) was offered to the experimental animals (the incomplete crossover design). Analysis of the chemical composition of saltwort showed that the plant contained high levels of total ash (40.2% DM), sodium (12.7% DM) and chlorine (19.7% DM), and relatively high levels of CP (11.7% DM) and NDF (40.4% DM). Contents of TDN and digestible CP (DCP), DE and ME of saltwort were 33.5% DM, 8.4% DM, 7.4MJ/DM kg and 5.0MJ/DM kg, respectively. These results indicate that saltwort is rich in DCP and minerals (mainly sodium chloride), but poor in energy, suggesting that saltwort could be used as a mineral or CP supplement for ruminants raised around salinized areas in which high quality feed may not be available.

A New Salt-Tolerant Thermostable Cellulase from a Marine Bacillus sp. Strain

  • dos Santos, Yago Queiroz;de Veras, Bruno Oliveira;de Franca, Anderson Felipe Jacome;Gorlach-Lira, Krystyna;Velasques, Jannaina;Migliolo, Ludovico;dos Santos, Elizeu Antunes
    • Journal of Microbiology and Biotechnology
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    • v.28 no.7
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    • pp.1078-1085
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    • 2018
  • A salt-tolerant cellulase secreted by a marine Bacillus sp. SR22 strain with wide resistance to temperature and pH was purified and characterized. Its approximate mass was 37 kDa. The endoglucanase, named as Bc22Cel, was purified by ammonium sulfate precipitation, gel filtration chromatography, and extraction from the gel after non-reducing sodium dodecyl sufate-polyacrylamide gel electrophoresis. The optimal pH value and temperature of Bc22Cel were 6.5 and $60^{\circ}C$, respectively. The purified Bc22Cel showed a considerable halophilic property, being able to maintain more than 70% of residual activity even when pre-incubated with 1.5 M NaCl for 1 h. Kinetic analysis of the purified enzyme showed the $K_m$ and $V_{max}$ to be 0.704 mg/ml and $29.85{\mu}mol{\cdot}ml^{-1}{\cdot}min^{-1}$, respectively. Taken together, the present data indicate Bc22Cel as a potential and useful candidate for industrial applications, such as the bioconversion of sugarcane bagasse to its derivatives.

Isolation and Characterization of Pb-Solubilizing Bacteria and Their Effects on Pb Uptake by Brassica juncea: Implications for Microbe-Assisted Phytoremediation

  • Yahaghi, Zahra;Shirvani, Mehran;Nourbakhsh, Farshid;de la Pena, Teodoro Coba;Pueyo, Jose J.;Talebi, Majid
    • Journal of Microbiology and Biotechnology
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    • v.28 no.7
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    • pp.1156-1167
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    • 2018
  • The aim of this study was to isolate and characterize lead (Pb)-solubilizing bacteria from heavy metal-contaminated mine soils and to evaluate their inoculation effects on the growth and Pb absorption of Brassica juncea. The isolates were also evaluated for their plant growth-promoting characteristics as well as heavy metal and salt tolerance. A total of 171 Pb-tolerant isolates were identified, of which only 15 bacterial strains were able to produce clear haloes in solid medium containing PbO or $PbCO_3$, indicating Pb solubilization. All of these 15 strains were also able to dissolve the Pb minerals in a liquid medium, which was accompanied by significant decreases in pH values of the medium. Based on 16S rRNA gene sequence analysis, the Pb-solubilizing strains belonged to genera Bacillus, Paenibacillus, Brevibacterium, and Staphylococcus. A majority of the Pb-solubilizing strains were able to produce indole acetic acid and siderophores to different extents. Two of the Pb-solubilizing isolates were able to solubilize inorganic phosphate as well. Some of the strains displayed tolerance to different heavy metals and to salt stress and were able to grow in a wide pH range. Inoculation with two selected Pb-solubilizing and plant growth-promoting strains, (i.e., Brevibacterium frigoritolerans YSP40 and Bacillus paralicheniformis YSP151) and their consortium enhanced the growth and Pb uptake of B. juncea plants grown in a metal-contaminated soil. The bacterial strains isolated in this study are promising candidates to develop novel microbe-assisted phytoremediation strategies for metal-contaminated soils.

Analysis of Salinity during the Growing Period in the Unripened Tidal Reclaimed Paddy Fields (간척초기답의 벼생육기간중 염분농도 분석)

  • Son, Jae-Gwon;Koo, Ja-Woong;Choi, Jin-Kyu
    • Journal of Korean Society of Rural Planning
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    • v.6 no.2 s.12
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    • pp.3-11
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    • 2000
  • The high salt concentration of reclaimed tidelands in the beginning of reclamation interferes with the growth of most crops. Although the crops are cultivated in the unripened tidal reclaimed paddy fields after desalinization to be arable, they we apt to be injured from salt by the resalinization through accumulated salts in the root zone during the growing period. In oder to make the reasonable irrigation plan in the unripened tidal reclaimed paddy fields, the preventive water requirements of resalinization as well as leaching requirements have to be included in irrigation water requirements. The critical salinity for the normal growth of crops should be determined to estimate the preventive water requirements of resalinization, and the changes of salinity in soil and water should be analyzed during the growing period, In this study, the growth tests of crops were conducted by soil textures and water management methods in the experimental field with lysimeters, using the samples of good drainage soils and poor drainage soils. And the changes of salinity in soil and water during the growing period, were analyzed to obtain the basic data for determining the critical salinity and making the estimation criteria of the preventive water requirements of resalinization. As the results obtained from analyzing the changes of salinity during the growing period in the unripened tidal reclaimed paddy fields, the exchanging interval of water for the prevention of resalinization was estimated to be within two weeks in good drainage soils and a week in poor drainage soils. And the total exchanging requirements of water for the prevention of resalinization during the growing period was estimated to be over 280mm in good drainage soils and 540mm in poor drainage soils.

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Detection of Wildfire-Damaged Areas Using Kompsat-3 Image: A Case of the 2019 Unbong Mountain Fire in Busan, South Korea

  • Lee, Soo-Jin;Lee, Yang-Won
    • Korean Journal of Remote Sensing
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    • v.36 no.1
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    • pp.29-39
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    • 2020
  • Forest fire is a critical disaster that causes massive destruction of forest ecosystem and economic loss. Hence, accurate estimation of the burned area is important for evaluation of the degree of damage and for preparing baseline data for recovery. Since most of the area size damaged by wildfires in Korea is less than 1 ha, it is necessary to use satellite or drone images with a resolution of less than 10m for detecting the damage area. This paper aims to detect wildfire-damaged area from a Kompsat-3 image using the indices such as NDVI (normalized difference vegetation index) and FBI (fire burn index) and to examine the classification characteristics according to the methods such as Otsu thresholding and ISODATA(iterative self-organizing data analysis technique). To mitigate the salt-and-pepper phenomenon of the pixel-based classification, a gaussian filter was applied to the images of NDVI and FBI. Otsu thresholding and ISODATA could distinguish the burned forest from normal forest appropriately, and the salt-and-pepper phenomenon at the boundaries of burned forest was reduced by the gaussian filter. The result from ISODATA with gaussian filter using NDVI was closest to the official record of damage area (56.9 ha) published by the Korea Forest Service. Unlike Otsu thresholding for binary classification,since the ISODATA categorizes the images into multiple classes such as(1)severely burned area, (2) moderately burned area, (3) mixture of burned and unburned areas, and (4) unburned area, the characteristics of the boundaries consisting of burned and normal forests can be better expressed. It is expected that our approach can be utilized for the high-resolution images obtained from other satellites and drones.

An Electrochemical Reduction of TiO2 Pellet in Molten Calcium Chloride (CaCl2 용융염에서 TiO2 펠렛의 전기화학적 환원반응 특성)

  • Ji, Hyun-Sub;Ryu, Hyo-Yeol;Jeong, Ha-Myung;Jeong, Kwang-Ho;Jeong, Sang-Mun
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.10 no.2
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    • pp.97-104
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    • 2012
  • A porous $TiO_2$ pellet was electrochemically converted to the metallic titanium by using a $CaCl_2$ molten salt system at $850^{\circ}C$. Ni-$TiO_2$ and graphite electrodes were used as cathode and anode, respectively. The electrochemical behaviour of $TiO_2$ pellet was determined by a constant voltage control electrolysis. Various reaction intermediates such as $CaTiO_3$, $Ti_2O$ and $Ti_6O$ were observed by XRD analysis during electrolysis of the pellet. Once $TiO_2$ pellet was converted to a porous metallic structure, the porous structure disappeared by sintering and shrinking with increasing the reaction time at high temperature.

Overexpression of a Pathogenesis-Related Protein 10 Enhances Biotic and Abiotic Stress Tolerance in Rice

  • Wu, Jingni;Kim, Sang Gon;Kang, Kyu Young;Kim, Ju-Gon;Park, Sang-Ryeol;Gupta, Ravi;Kim, Yong Hwan;Wang, Yiming;Kim, Sun Tae
    • The Plant Pathology Journal
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    • v.32 no.6
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    • pp.552-562
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    • 2016
  • Pathogenesis-related proteins play multiple roles in plant development and biotic and abiotic stress tolerance. Here, we characterize a rice defense related gene named "jasmonic acid inducible pathogenesis-related class 10" (JIOsPR10) to gain an insight into its functional properties. Semi-quantitative RT-PCR analysis showed up-regulation of JIOsPR10 under salt and drought stress conditions. Constitutive over-expression JIOsPR10 in rice promoted shoot and root development in transgenic plants, however, their productivity was unaltered. Further experiments exhibited that the transgenic plants showed reduced susceptibility to rice blast fungus, and enhanced salt and drought stress tolerance as compared to the wild type. A comparative proteomic profiling of wild type and transgenic plants showed that overexpression of JIOsPR10 led to the differential modulation of several proteins mainly related with oxidative stresses, carbohydrate metabolism, and plant defense. Taken together, our findings suggest that JIOsPR10 plays important roles in biotic and abiotic stresses tolerance probably by activation of stress related proteins.

Permeation Property of Ionomer Film with New Multifunctional Ionic Site (다관능기를 도입한 아이오노머 필름의 기체투과 특성)

  • Lee, Bo-Mi;Jeong, Sam-Bong;Nam, Sang-Yong
    • Korean Journal of Materials Research
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    • v.22 no.5
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    • pp.227-236
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    • 2012
  • Ionomer is a thermoplastic that is composed of covalent bonds and ionic bonds. It is possible to use this material in processes such as injection molding or extrusion molding due to the material's high oil resistance, weatherproof characteristics, and shock resistance. In this study, a new ionomer having a multifunctional group was prepared by a stepwise neutralization system with the addition of acidic and salt additives. In step I, to increase the contents of the multifunctional group and the acid degree in ethylene acrylic acid (EAA), MGA was added to the ionomer resin (EAA). A new ionomer was prepared via the traditional preparation method of the ionic cross-linking process. In step II, metal salt was added to the mixture of EAA and MGA. The extrusion process was performed using a twin extruder (L/D = 40, size : ${\varphi}30$). Ionomer film was prepared for evaluation of gas permeability by using the compression molding process. The degree of neutralized and ionic cross-linked new ionomer was confirmed by FT-IR and XRD analysis. In order to estimate the neutralization of the new ionomer film, various properties such as gas permeation and mechanical properties were measured. The physical strength and anti-scratch property of the new ionomer were improved with increase of the neutralization degree. The gas barrier property of the new ionomer was improved through the introduction of an ionic site. Also, the ionic degree of cross-linking and gas barrier property of the ionomer membrane prepared by stepwise neutralization were increased.

Formulation Of Some Mathematical Models For The Estimations Of The Most Probable Salts Derived From The Major Mineral Constituents In Natural Water

  • Miah, Raisuddin
    • Analytical Science and Technology
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    • v.8 no.4
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    • pp.759-770
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    • 1995
  • By extensive studies with the quantitative status of the mineral constituents of thousands of water samples, it was found that almost in all natural waters irrespective of the surface or sub-surface sources, minimum 99.5% of the total amount of the cationic constituents are generally the contributions of 3 commonly present parameters viz. Ca, Mg and Na and that of the anionic species are same and contributed by $HCO_3$, Cl and $SO_4$ only. In the field of water works, all these major mineral substances are conventionally measured as their individual ions. But till now, no reliable and generalised methods or rules have been developed for the determination of the exact kinds of the individual salt components and their amounts from these ionic constituents normally present in water. As salt content, only the TDS (Total Dissolved Solids) parameter is frequently measured by evaporation of the water sample. But TDS can tell nothing about the kinds and amounts of the individual salts present in it. Considering the analytical importance of the estimation of the mineral substances as their individual salts, some generalised mathematical models have been developed by this research which are based on the 'hypothetical order of chemical combinations' as may occur among the ionic constituents. With the help of these models, one can easily assume the most probable salts with approximate quantities derived from the ionic constituents. In addition, approximate amount of Na content can also be estimated mathematically with simultaneous verification of the correctness of the water analysis results. The models are stated in this paper with practical illustrations and descriptions of the method of applications.

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Physicochemical Properties of Depth-based Soil on the Reclaimed Dredging Area (임해준설매립지 토양의 깊이별 이화학적 특성)

  • Nam, Woong;Kwak, Young-Se;Jeong, In-Ho;Lee, Deok-Beom;Lee, Sang-Suk
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.11 no.5
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    • pp.60-71
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    • 2008
  • Through analyzing the specific physicochemical alterations in depth-based soil at reclaimed dredging area, the baseline data were provided for developing the reclaimed dredging area as natural landscape planting sites and ecological-landscape sites based on the soil improvement technology. There was no physical disturbance for 15 to 20 years in Gwangyang Bay reclaimed dredging area after reclamation. Physicochemical examinations of the soil were performed based on the vertical depth. Results of physicochemical analysis such as pH, electric conductivity, total salt contents, silt, clay contents, available phosphorus, calcium, magnesium, sodium, chlorine, and sodium-adsorption ratio showed increasing patterns with the depth while total organic contents, total nitrogen, and sand showed decreasing patterns. Potassium as an exchangeable cation, showed similar distribution patterns between the shallow and deep soil. This result strongly implied that long-term exposure to natural rainfall in reclaimed dredging area altered soil characteristics related to salinity. This research demonstrated that there were no remarkable differences in physicochemical characteristics at soil depth and groundwater table height, suggesting a baseline data for developing reclaimed dredging area. Additional investigation is required for different reclaimed dredging areas. Also, additional monitoring and examination are need on plant communities and time variable alteration in the soil to test the feasibility of reclaimed dredging areas as natural landscape planting sites and ecological-landscape sites.